FOOD CHEMISTRY
·
2013
·
IF 3.334
Juan Zhang,Lin Xiao,Yucai Yang,Zhaoxia Wang,Genxi Li
In this paper, we find that the effect of lignin on pancreatic lipase (PL) is dependent on reaction medium and substrate used. Experimental results reveal that lignin can gradually bind to PL to form a PL–lignin complex, resulting in an increased activity of the enzyme. The binding process is spontaneous and the PL–lignin complex formation is an endothermic reaction induced by hydrophobic and electrostatic interaction. There is a non-radiation energy transfer from PL to lignin during the binding process, and the binding of lignin to PL conforms to a secondary exponential decay function. Moreover, the α-helix content of the enzyme will be changed and the rigidity of its side chain will be enhanced due to the formation of lignin–PL complex. This study has not only provided the activation effect of lignin on PL, but also given an insight into the interaction between lignin and the enzyme, which would benefit the application of lignin in the pharmacy and food industry, as well as other fields.
研究领域:
生物化学
·
酶学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Xinlin Chen,Shang Xue,Yaolan Lin,Jianguang Luo,Lingyi Kong
In terms of ligand fishing, the amount and the relative activity recovery of enzymes immobilized on magnetic particles and nanoparticles are not preeminent. Therefore, the metal-organic framework (MOF) UiO-66-NH 2 was synthesized to immobilize the porcine pancreatic lipase (PPL) via precipitation-cross-linking, and the resulting novel biological matrices named PPL@MOF manifested high PPL loading capacity (98.31 mg/g) and relative activity recovery (104.4%). Moreover, the novel enzyme-MOF composite was applied to screen lipase inhibitors from Prunella vulgaris L. to enrich and improve the techniques of ligand fishing. As a result, 13 lipase ligands were obtained, and 12 compounds were determined by HPLC-Q-TOF-MS/MS. All of these ligands were further confirmed to be potential inhibitors through the verification of the activity assay and molecular docking . The proposed approach based on PPL@MOF was superior in terms of abundant protein loading capacity, high enzyme catalytic activity and easy preparation process. Taken together, our newly developed method provided a new platform for efficient discovering bioactive molecules from natural herbs.
研究领域:
生物化学
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药物发现
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yi-Ting Chen,Peng-Tai Long,Hai-Xia Xu,Wen-Jun Wang,Qing-Feng Zhang
Inhibition of pancreatic lipase (PL) is a strategy to prevent obesity. The inhibitory effects of Flos Sophorae Immaturus (FSI) extract and its main flavonoid components, rutin and quercetin, on PL were investigated. The contents of rutin and quercetin in FSI extract were 44.10 ± 1.33 % and 6.07 ± 1.62 %, respectively. The IC 50 values of FSI extract, rutin and quercetin on PL were 322, 258 and 71 μg/mL, respectively. Rutin and quercetin inhibited PL in a reversible and noncompetitive manner. The combination of rutin and quercetin exhibited synergistic inhibitory effects at low concentration. The binding of rutin/quercetin with PL caused the fluorescence quenching of protein. Fluorescence titration showed the binding affinity of quercetin with PL protein was stronger than that of rutin. Circular dichroism analysis showed the binding changed the secondary structure of PL with an increase in random coil and a decrease in α-Helix and β-Sheet. Molecular docking revealed that rutin and quercetin could interact with the amino acid residues around the catalytic site through multiple secondary interactions. In vivo studies showed that FSI extract can reduce fat absorption and promote fecal fat excretion through inhibition of PL activity, and the effects were mainly due to rutin and quercetin.
研究领域:
药理学
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生物化学
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营养学
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2017
·Vol. 185
·期 3
·606-618
·
IF 1.751
Zou Bin,Chu Yanhong,Xia Jiaojiao,Chen Xueshan,Huo Shuhao
Porcine pancreatic lipase (PPL) was immobilized onto functionalized ionic liquid-modified silica carrier using gelatinization and physical adsorption. The immobilized lipase was characterized with N 2 adsorption–desorption, X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FT-IR) before and after modification and immobilization. The results showed that the modification of the ionic liquid and the introduction of lipase had been successfully approved. The rate of enzymatic reaction and its influencing factors was primarily studied by enzymatic reaction kinetics. K
m values of PPL-SiO 2 @CA and PPL-IM/BF 4 -SiO 2 @CA were 4.9 and 3.7 mg/ml, respectively. It indicated that the modification of the functionalized ionic liquid enhanced the affinity between the immobilized enzyme and the substrate. The immobilization efficiency, specific activity, optimum temperature, optimum pH, thermal stability, reusability, and storage stability of the immobilized enzyme were investigated. We found that the stability of the immobilized enzyme was significantly higher than that of the unmodified immobilized enzyme. Specially, PPL-IM/BF 4 -SiO 2 @CA maintained good thermal stability and retained more than 92% of its activity at 65 °C after preheating 3 h.
Above, the immobilized lipase maintained more than 92% of its initial activity after incubating at 65 °C for 3 h.
研究领域:
生物化学
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酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Chunhua Zhu,Tao Hong,Mingjing Mou,Yanhong Chen,Zhipeng Li,Mingjing Zheng,Zedong Jiang,Hui Ni,Yanbing Zhu
The immobilized κ-carrageenase MCNF was developed by synergistic integration of κ-carrageenase, calcium phosphate crystals, and magnetic nanoparticles. MCNF outperformed free κ-carrageenase in terms of optimal temperature, thermostability (45–55 °C), stability (pH 6.0 and 12.0), and storage stability. Furthermore, MCNF was extremely reusable and easy to separate from the reaction system. MCNF’s enzymatic breakdown of κ-carrageenan resulted in a tetrasaccharide that competitively inhibited pancreatic lipase (PL). Fluorescence titration experiments showed that κ-carrageenan tetrasaccharide altered the microenvironment of PL by causing static bursting of its intrinsic fluorescence. The circular dichroism experiment demonstrated that adding κ-carrageenan tetrasaccharide reduced α-helix content and increased β-sheet content in PL’s secondary structure. Molecular docking and dynamics simulation research suggested that the κ-carrageenan tetrasaccharide could form a stable complex with PL, entering its hydrophobic cavity and occupying its active site, reducing PL’s catalytic activity. κ-Carrageenan tetrasaccharide could inhibit PL, making it a promising therapeutic agent for obesity prevention and treatment.
研究领域:
食品科学
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生物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2021
·
IF 4.24
Jian-Feng Zhou,Wen-Jun Wang,Zhong-Ping Yin,Guo-Dong Zheng,Ji-Guang Chen,Jing-En Li,Ling-Li Chen,Qing-Feng Zhang
The inhibitory activity of quercetin on pancreatic lipase was investigated in both vitro and vivo. Quercetin exhibited inhibitory effect on pancreatic lipase with IC 50 value of 70 μg/mL. Inhibitory kinetics indicated that quercetin was more like a mixed-type (non-competitive) inhibitor of pancreatic lipase. Quercetin induced the endogenous fluorescence quenching of lipase, which suggested the interaction between them. The binding constant and the number of the binding sites were further calculated. Molecular docking results showed that quercetin had a binding site on lipase near the active pocket. The binding of quercetin on lipase would affect its conformation, thereby decreasing the affinity between the substrate and the enzyme . In vivo studies showed that pre-administration with 5 and 10 mg/kg bwt quercetin could significantly reduce fat absorption in rat. The inhibition of quercetin on lipase can last at least 2 h in vivo. Correspondingly, quercetin significantly increased fat excretion in rat feces. The change trends of fat and quercetin content in rat feces were similar.
研究领域:
药理学
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生物化学
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营养学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yujie Wang,Juan Cheng,David Julian McClements,Jing Chen,Da Ma
This study explores the inhibitory effects of two migrants—2-mercaptobenzothiazole (MBT) and stearic acid—from dairy rubber contact materials on pancreatic lipase (PL). Enzyme assays showed significant, dose-dependent inhibition, with stearic acid being more potent. MBT and stearic acid exhibited reversible inhibition, with MBT acting competitively and stearic acid non-competitively. Fluorescence spectroscopy and circular dichroism indicated conformational changes in PL upon binding, while molecular docking confirmed specific interactions at the active site (MBT) and near it (stearic acid). Binding affinities for MBT-PL and stearic acid-PL complexes were − 24.90 and − 20.23 kJ/mol, respectively. High concentrations of both compounds exhibited synergistic inhibition, potentially affecting lipid digestion in dairy products. This study highlights the influence of rubber-derived migrants on PL activity and suggests limiting their use in food contact materials to safeguard dairy nutritional quality.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CATALYSIS LETTERS
·
2019
·Vol. 150
·期 5
·1268-1276
·
IF 2.372
Yang Zeng-Jie,Wang Na,He Wei-Xun,Yu Yuan,Gong Qing-Tian,Yu Xiao-Qi
Lipase from porcine pancreas (PPL) is first reported to catalyze direct 1,6-conjugated addition for synthesis of triarylmethanes using p -quinonemethides ( p -QMs) and 2-naphthols. The catalytic activity of PPL was evaluated through investigating the solvent, the ratio of substrates, the enzyme loading and the temperature of the enzyme-catalyzed reactions. The present method proves to be environmentally friendly and efficient in terms of high yield, green catalyst and simple synthesis method. GraphicAbstract
研究领域:
生物化学
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有机合成
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酶催化
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Wen-Yu Liu,Xin-Yue Jia,Hao-Yue Zheng,Hui-Hui Zhang,Jun-Xia Xiao,Guo-Qing Huang
The Pickering emulsion stabilized by ovalbumin/pectin polyelectrolyte complex with porcine pancreatic lipase (PPL) dissolved in the aqueous phase was investigated for its feasibility in biphasic catalysis. CLSM and QCM-D analysis revealed that PPL discretely adsorbed to emulsion interface, though they were both negatively charged. The hydrolysis of p-NPP was favored in low stabilizer concentrations and could be saturated by PPL, and the optimum conditions were pH 6.5, ovalbumin to pectin mass ratio 1:3, complex concentration 0.25 %, and PPL dosage 0.6 %. Under these conditions, the Pickering emulsion conferred a conversion rate of 0.87 μmol/h and product yield of 46.8 % after reaction for 5 h, which were significantly lower than 1.37 μmol/h and 73.6 % of the conventional oil/water system. Hence, Pickering emulsions stabilized by protein/polysaccharide polyelectrolyte complexes might not be ideal media for biphasic catalysis, possibly due to the viscoelastic property of the complexes that could better cover emulsion interfaces.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2019
·Vol. 54
·期 6
·2084-2093
·
IF 2.281
Mohamed Abdin,Yahya Saud Hamed,Hafiz Muhammad Saleem Akhtar,Dan Chen,Shanza Mukhtar,Peng Wan,Asad Riaz,Xiaoxiong Zeng
Summary The objectives of this study were to determine the optimal extraction conditions of polyphenols from Syzygium cumini seeds by response surface methodology and investigate their antioxidant activity and inhibition on α-amylase and pancreatic lipase. As results, the optimal extraction conditions in the ultrasonic extraction process which maximised total polyphenols content, minimised the IC 50 values of α-amylase and pancreatic lipase were determined as follows: extraction time 60 min, ethanol concentration 63% and solvent/solid ratio 44 mL g −1 . The main phenolic compounds in partially purified fraction of Syzygium cumini seeds were catechin, epicatechin, kaempferol, gallic, 5-caffeoylquinic, caffeic and ferulic acids. In addition, the partially purified fraction inhibited 87.66 ± 5.55 and 86.61 ± 3.15% of α-amylase and pancreatic lipase, respectively. The results suggested that Syzygium cumini seeds could be explored as a natural antioxidant and could be used as a source of highly antidiabetic and anti-obesity bioactive compounds.
研究领域:
食品科学
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药理学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Rui Huang,Yu Zhang,Shuyu Shen,Zijian Zhi,Huan Cheng,Shiguo Chen,Xingqian Ye
Obesity and oxidative damage are two important risk factors associated closely with metabolic syndrome. Utilization of functional food ingredients is considered as a feasible way to tackle these challenges. In the present study, eight representative species of citrus peel extracts (CPEs) were evaluated and compared for their flavonoid profiles, antioxidant activities, and pancreatic lipase (PL) inhibitory capacities and mechanisms. Results indicated that hesperidin, naringin, neohesperidin, narirutin and eriocitrin were the five major flavonoids in CPEs, among which hesperidin was the main active PL inhibitor. Moreover, hesperidin could interact with PL by hydrogen bonds and van der Waals forces , and the interaction would not obviously change the secondary structure of PL. Overall, ponkan peel extract, having the strongest overall antioxidant activity, the highest content of hesperidin and total phenolic compounds among all tested CPEs, is a promising natural ingredient to scavenge free radicals and manage obesity.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2019
·Vol. 35
·期 2
·235-238
·
IF 1.071
Jiang Ling,Ye Wenting,Su Weike
A series of highly functionalized 1,4-dihydropyridines was synthesized via one-pot multicomponent reactions of aromatic aldehyde, malononitrile and N -methyl-1-(methylthio)-2-nitroethenamine using Porcine pancreatic lipase(PPL) as catalyst in DMSO. This protocol is featured by mild reaction conditions, simple operation and environmental acceptability.
研究领域:
有机化学
·
催化化学
·
绿色化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）