RSC Advances
·
2020
·Vol. 10
·期 47
·28041-28048
·
IF 3.119
Caixia Liu,Baiping Mao,Vanessa Martinez,Xiaojian Chen,Yanan Li,Lingyun He,Sian Chen,Xiaoling Guo,Xian Shen,Xiandan Bao,Haifa Shen,Stefania Lenna,Pinyi Qian,Lingzhi Wu,Chao Li
The outbreak of new coronavirus disease (COVID-19) has quickly spread all over the world. Real time reverse transcriptase polymerase chain reaction (rRT-PCR) for nucleic acid detection has become the standard method for clinical diagnosis of COVID-19 infection. But these rRT-PCR tests have many inherent limitations, and carry a high false negative rate. It is an urgent to develop a method to accurately identify the vast infected patients and asymptomatic viral carriers from the population. In this article, we present the principle and procedure of developing a colloidal gold immunochromatographic assay (GICA) for rapid detection of COVID-19-specific antibodies. The detection kit can be used to detect immunoglobulin M (IgM) and IgG of COVID-19 in human blood samples within 15 minutes, and to identify different stages of viral infection. Test results can be digitalized using an office scanner and a FiJi software with appropriate confidence interval (CI) setting. Based on analysis from 375 samples, we calculated that overall sensitivity and specificity of the assay were 95.85% and 97.47%, respectively. Compared with rRT-PCR, this assay has many advantages including convenience and rapid detection. The detection kit can be widely used in hospitals, clinics and laboratories for rapid screening of both symptomatic and asymptomatic COVID-19 carriers in large scale.
研究领域:
医学
·
病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Jialin Hu,Xinxin Xu,Liguang Xu,Hua Kuang,Chuanlai Xu,Lingling Guo
Wheat gliadin is one of the most important causes of gluten sensitivity. This study aimed to develop a sandwich enzyme-linked immunosorbent assay (ELISA) and gold nanoparticle-based lateral flow (LFIA) strips to detect wheat allergen in food. Through cell screening, ten monoclonal antibodies (mAbs) against gliadin and after optimization were obtained, a pair of mAbs suitable for its detection (capture antibody: mAb 7; detection antibody : HRP-labeled mAb 6) were identified. Based on two of these antibodies, a sandwich ELISA with limit of detection (LOD) of 60 ng/mL in negative milk, and more importantly, negligible cross-reactivity to other allergens was developed. The average recoveries for gliadin in negative milk were 99.16%–100.07% using the sandwich ELISA. We also developed LFIA strips for the rapid detection of gliadin with visual limit of detection (vLOD) of 25 ng/mL and calculated LOD value of 6.56 ng/mL in negative milk. The test results of positive samples obtained from the LFIA strips were highly consistent with those of the sandwich ELISA. Thus, the developed LFIA strip is an effective and reliable tool for the rapid and on-site detection of wheat allergen in milk.
研究领域:
食品科学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2025
·
IF 5.7
Jingqi Dong,Jialin Hu,Liqiang Liu,Aihua Qu,Hua Kuang,Chuanlai Xu
Kynurenine (KYN), a metabolite of tryptophan, is currently receiving increasing attention as one of the biomarkers for clinical disease monitoring. To realize the determination of KYN in human plasma samples in the clinic, a specific monoclonal antibody (mAb) against KYN was prepared based on the structural characteristics of KYN. By optimizing the detection conditions, we obtained anti-KYN mAb 1G10 with a sensitivity of 143.66 ng/mL, and established a colloidal gold immunochromatographic assay (CGCA) platform for the qualitative and quantitative analysis of KYN in human plasma samples, using CGCA strips to meet the needs of clinical testing. The recoveries of the strips for the determination of KYN in plasma samples were in the range of 85.22%–99.72%, which were validated by liquid chromatography–tandem mass spectrometry (LC-MS/MS), and results were in good agreement with the LC-MS/MS method, indicating that the accuracy of the prepared CGCA strips was high. Based on the characteristics of low cost, high throughput and good specificity, the test strips will be widely applicable for clinical determination.
研究领域:
医学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 6
·期 2
·1279-1287
·
IF 3.84
Xuan Zhang,Jun Zhou,Chundan Zhang,Dijun Zhang,Xiurong Su
To establish the rapid detection of Enterobacter cloacae in food, immunomagnetic beads were prepared by coupling an anti-E. cloacae polyclonal antibody with magnetic beads. An immunochromatographic test strip was composited with an anti-E. cloacae monoclonal antibody marked by colloidal gold as the detection antibody, anti-E. cloacae polyclonal antibody as the test line, and donkey anti-mouse IgG secondary antibody as the control line. Immunomagnetic separation was combined with a colloidal gold-based immunochromatographic assay for the rapid detection of E. cloacae. The results showed that 102 CFU mL−1 E. cloacae could be detected using the immunochromatographic test strip after immunomagnetic separation. The sensitivity was 10 times higher than direct detection with an immunochromatographic test strip. With the immunochromatographic test strip, water samples spiked with E. cloacae yielded a negative result, whereas E. cloacae yielded a positive result with immunomagnetic separation. The proposed method enhances the rapid detection of E. cloacae in food.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Yuxi Lu,Xuelu Gao,Jinming Song,Chen-Tung Arthur Chen,Jinling Chu
The environmental characteristics of colloidal toxic trace metals Cd, Cu and Pb in riverine and estuarine waters collected from two urban rivers of Yantai City in eastern China, the Guangdang and Xin'an Rivers, were investigated using a modified centrifugal ultrafiltration (CUF) method in conjunction with acid extraction and inductively coupled plasma mass spectrometry. The target metals in dissolved pool were divided into four CUF fractions, i.e. <1 kDa, 1–3 kDa, 3–10 kDa and 10 kDa–0.2 μm, and the results showed that colloidal Cd, Cu and Pb were dominated by 1–10 kDa (1–3 and 3–10 kDa), 1–3 kDa and 10 kDa–0.2 μm fractions, respectively. The coagulation/flocculation of low-molecular-weight (1–10 kDa) colloidal Cd and Cu in the estuaries was obvious and strong, while the enrichment of dissolved Pb in the 10 kDa–0.2 μm fraction may be mainly related to its biogeochemical interactions with Fe-oxides, which is easy to occur in macromolecular colloids. In addition, the actual molecular weight cutoffs (MWCOs) of the three used CUF units with nominal MWCOs of 1, 3 and 10 kDa were determined to be 4.9, 8.5 and 33.9 kDa, respectively, indicating that membrane calibration is essential for explaining the actual fraction of dissolved trace metals and verifying the integrity of ultrafiltration membrane. Overall, the results in this study provide a further understanding of the heterogeneity in biogeochemical features, migration and fate of toxic trace metals in aquatic ecosystems, especially that of the river-sea mixing zone.
研究领域:
环境科学
·
海洋学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2022
·
IF 1.193
Xiaoqian JIANG,Xinxin XU,Shanshan SONG,Hua KUANG,Liqiang LIU,Liguang XU,Chuanlai XU
Digitoxin is a cardiac glycoside used in the therapy of heart failure and arrhythmias, and has potential as an anti-cancer and anti-influenza treatment. However, digitoxin has the disadvantage of a narrow therapeutic window meaning that it is easy to cause toxicity. Consequently, there is an urgent need to accurately monitor the plasma concentration of patients. In this study, an anti-digitoxin monoclonal antibody (mAb) 5D2 was developed and purified, and found to have outstanding specificity, sensitivity, and affinity. The half inhibitory concentrations (IC 50 ) and the limit of detection (LOD) values of mAb 5D2 in human plasma determined by ic-ELISA were 0.205 ng/mL and 0.010 ng/mL, while the cross-reactivity with other analogs was negligible. Based on mAb 5D2, a colloidal gold-based immunochromatographic assay was developed for the purpose of quickly monitoring the concentration of digitoxin in human plasma. The detection results could be obtained within 10 min, and were in agreement with those of liquid chromatography-tandem mass spectrometry (LC-MS/MS). The strip could be evaluated semi-quantitatively by the naked eye, with a visual LOD (vLOD) of 0.5 ng/mL and a cut-off value of 10 ng/mL. Meanwhile, the linear range was between 0.319 ng/mL and 2.406 ng/mL, which was obtained quantitatively by the strip reader. Therefore, the immunochromatographic strip could offer a new tool for rapid, accurate, and portable detection of digitoxin.
研究领域:
医学
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药理学
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免疫学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Lu Zeng,Huan Lv,Xiangrui Li,Wenjia Yang,Wencan Luo,Linghong Kong,Qiujin Zhu
Cyclosulfamuron (CS) is a widely used sulfonylurea herbicide in plant cultivation and food production, and poses significant threats to ecological balance and food safety. To address this concern, we designed a novel CS hapten using computer-simulated semi-antigens. A monoclonal antibody (mAb) exhibiting high specificity and affinity was generated, with an affinity constant of 1.049×10 9 L/mol and a semi-inhibitory concentration (IC 50 ) of 4.94 ng/mL. Leveraging this anti-CS mAb, we constructed a rapid, visually detectable, quantitative lateral flow immunoassay (LFIA) to detect CS in cereals. Under the optimal conditions, the cut-off values were determined to be 100 μg/kg for brown rice and corn samples and 50 μg/kg for rice samples. The corresponding limits of detection values (LODs) were found to be 1.93 μg/kg in brown rice, 2.18 μg/kg in corn and 1.93 μg/kg in rice, respectively. The recovery rates ranged from 96.2% to 104.8% with coefficients of variation ranging from 3.5% to 6.4%, showing good agreement with the results obtained by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The proposed assay demonstrated robust analytical performance, with significant application potential for rapid, on-site detection.
研究领域:
食品安全
·
分析化学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 96
·78833-78840
·
IF 3.84
Lin Luo,Jin-Yi Yang,Zhi-Li Xiao,Dao-Ping Zeng,Yong-Jun Li,Yu-Dong Shen,Yuan-Ming Sun,Hong-Tao Lei,Hong Wang,Zhen-Lin Xu
Histamine (HA) is an essential test item for fishery samples. However, the fast and effective determination of HA is difficult due to its simple structure and small molecular size. In this study, a sensitive and specific monoclonal antibody against p-nitrobenzoylated histamine (NPHA), which can be easily obtained from the reaction of HA and p-nitrobenzoic acid N-hydroxysuccinimide ester (PNBA-OSu) under mild conditions, was generated for the first time. Based on this mAb, an immunochromato-graphic assay strip (ICA strip) using a colloidal gold nano particles-antibody (GNPs) probe for rapid detection of HA in saury samples was established. After screening the coating antigens and optimization of analytical parameters, a heterologous coating based ICA strip exhibited the most excellent detection ability with a visual detection limit (VDL) of 6.0 mg kg−1 in qualitative experiments and a detection limit (by a strip reader) of 1.0 mg kg−1 in semi-quantitative experiments for saury samples, with no cross-reactivity with HA analogs. Good correlation between the ICA strip with liquid chromatography-tandem mass spectrometry was achieved for spiked and naturally contaminated saury samples. Overall, this method is suitable for screening of HA residue for large scale fish samples in a quick, simple and low-cost manner.
研究领域:
分析化学
·
食品安全
·
免疫学
·
分子诊断
·
兽药残留检测
✓ 已验证
DOI
高影响因子
ARCHIVES OF VIROLOGY
·
2021
·Vol. 166
·期 3
·871-879
·
IF 2.574
Zhang Xinyu,Liu Xiaoyu,Wu Xiaodong,Ren Weijie,Zou Yanli,Xia Xiaoli,Sun Huaichang
African swine fever (ASF), caused by African swine fever virus (ASFV), was first reported in Kenya in 1921, but an effective vaccine or antiviral drug is still not available for ASFV control. Rapid and effective diagnostics are key steps in managing ASF. We generated two monoclonal antibodies (MAbs) against the ASFV phosphoprotein P30 and designated these as 3H7A7 and 6H9A10. Epitope mapping revealed that MAb 3H7A7 and 6H9A10 recognized aa 144-154 and aa 12-18 of P30, respectively. A signal-amplified sandwich colloidal gold test strip for rapid detection of ASFV was developed based using these MAbs. Sensitivity and specificity analysis showed that the detection limit of the strip was 2.16 ng of P30. The strip only reacted with ASFV and did not react with other common porcine viruses. In detection tests using 153 clinical field samples including sera, plasma, anticoagulant-treated blood, and tissue, the strip had 95.42% concordance with real-time PCR. The new MAbs specific for P30 and the rapid colloidal gold test strip helped to reveal novel B cell epitopes in P30 and provide an efficient diagnostic test for on-site clinical detection of ASF.
研究领域:
兽医学
·
分子生物学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaofang Li,Liqiang Liu,Shanshan Song,Maozhong Sun,Hua Kuang,Chuanlai Xu,Lingling Guo
Aflatoxins (AFTs) are highly carcinogenic and mainly contaminate cereals. In this study, we designed and screened a hapten by analyzing the same characteristic groups of AFTs (AFB 1 , AFB 2 , AFG 1 and AFG 2 ), prepared a monoclonal antibody (mAb) 4C11 with a high sensitivity and specificity that can simultaneously detect all the main AFTs, and established a colloidal gold immunochromatographic assay (CG-ICA) for the detection of AFTs in cereals. The limits of detection (LODs) were 0.77 μg/kg, 1.40 μg/kg and 0.71 μg/kg for wheat, rice and maize, respectively, with linear ranges of 1.17–5.52 μg/kg, 1.84–5.20 μg/kg and 1.26–8.95 μg/kg, respectively. The spiked recoveries of AFTs samples ranged from 95.6 % to 104.2 %, and the positive samples further validated the reliability of the method. The results demonstrated that the method can be used for rapid, sensitive and efficient on-site detection of AFTs in cereals.
研究领域:
食品安全
·
免疫学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC MICROBIOLOGY
·
2021
·Vol. 21
·期 1
·1-10
·
IF 3.605
Xu Meng,Lu Feifeng,Lyu Chenang,Wu Qingping,Zhang Jumei,Tian Peng,Xue Liang,Xu Ting,Wang Dapeng
Background Human noroviruses (HuNoVs) are a major cause of nonbacterial gastroenteritis in all age groups worldwide. HuNoVs can be detected in vitro using molecular assays such as RT-PCR and RT-qPCR. However, these molecular-based techniques require special equipment, unique reagents, experienced personnel, and extended time to obtain results. Besides, the diversity of viral genotypes is high. Therefore, methods that are rapid, broad-range and effective in the detection of HuNoVs are desiderated for screening the feces or vomit of infected people during outbreaks. Results In this study, a colloidal-gold-based immunochromatographic assay (ICA) was developed for effective detection of HuNoVs in clinical samples. Monoclonal antibodies (MAbs) against the shell (S) domain in the major capsid protein of HuNoVs were used in the ICA. The limitations of detection for HuNoVs in clinical samples were 1.2 × 10 6 genomic copies per gram of stool sample (gc/g) and 4.4 × 10 5 gc/g for genogroup I and II (GI and GII) HuNoVs, respectively. A total of 122 clinical samples were tested for HuNoVs by ICA and compared against RT-qPCR. The relative sensitivity, specificity and agreement of ICA was 84.2% (95% CI: 83.6–84.8%), 100.0% (95% CI: 98.5–100.0%) and 87.7% (95% CI: 85.6–89.8%), respectively. No cross-reaction with other common enteric viruses or bacteria was observed. The ICA detected a broad range of genotypes, including GI.1, GI.3, GI.4, GI.6, GI.14, GII.2, GII.3, GII.4, GII.6, GII.13, and GII.17 HuNoVs. Conclusions This study demonstrates that ICA targeting the S domain of VP1 is a promising candidate for effectively identifying the different genotypes of HuNoVs in clinical samples with high sensitivity and specificity.
研究领域:
医学诊断
·
病毒学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2015
·
IF 0.986
Naifeng Xu,Liguang Xu,Wei Ma,Liqiang Liu,Hua Kuang,Chuanlai Xu
A non-pretreatment monitoring method based on immunochromatographic test (ICT) strips was developed to test for chloramphenicol (CAP) in raw milk. This assay was designed to measure competitive binding affinities for CAP molecules in raw milk and CAP antigen immobilized on strips with colloidal gold nanoparticles (GNPs, average diameter of 30 nm) labeled with anti-CAP monoclonal antibody (CAP mAb). Several working conditions, including the CAP solvent, different types and concentrations of CAP mAb, coating antigen, and GNP, were optimized for performance. After optimization, the detection process was carried out in 8 min with a visual limit of detection (LOD) of 0.3 ng/mL for qualitative detection and a LOD of 0.064 ng/mL for semi-quantitative detection. No cross-reactivity was detected toward CAP analogs. Based on these results, this simple and ultrasensitive assay could be thoroughly applied in the on-site testing of CAP in raw milk.
研究领域:
食品安全检测
·
分析化学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）