Marine Drugs
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2021
·Vol. 19
·期 12
·676
·
IF 5.118
Jun-Hui Cheng,Xiao-Yu Zhang,Zhen Wang,Xia Zhang,Shi-Cheng Liu,Xiao-Yan Song,Yu-Zhong Zhang,Jun-Mei Ding,Xiu-Lan Chen,Fei Xu
Bovine bone is rich in collagen and is a good material for collagen peptide preparation. Although thermolysin-like proteases (TLPs) have been applied in different fields, the potential of TLPs in preparing bioactive collagen peptides has rarely been evaluated. Here, we characterized a thermophilic TLP, A69, from a hydrothermal bacteriumAnoxybacillus caldiproteolyticus1A02591, and evaluated its potential in preparing bioactive collagen peptides. A69 showed the highest activity at 60 °C and pH 7.0. We optimized the conditions for bovine bone collagen hydrolysis and set up a process with high hydrolysis efficiency (99.4%) to prepare bovine bone collagen peptides, in which bovine bone collagen was hydrolyzed at 60 °C for 2 h with an enzyme–substrate ratio of 25 U/g. The hydrolysate contained 96.5% peptides that have a broad molecular weight distribution below 10000 Da. The hydrolysate showed good moisture-retention ability and a high hydroxyl radical (•OH) scavenging ratio of 73.2%, suggesting that the prepared collagen peptides have good antioxidative activity. Altogether, these results indicate that the thermophilic TLP A69 has promising potential in the preparation of bioactive collagen peptides, which may have potentials in cosmetics, food and pharmaceutical industries. This study lays a foundation for the high-valued utilization of bovine bone collagen.Keywords:thermolysin-like protease;bovine bone collagen;collagen peptides;collagen peptide preparation;bioactivity
研究领域:
生物化学
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食品科学
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药学
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化妆品
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产品关联
DOI
PubMed
高影响因子
Marine Drugs
·
2023
·Vol. 21
·期 10
·518
·
IF 5.4
Qianxia Lin,Yueping Guo,Jie Li,Shuqi He,Yan Chen,Huoxi Jin
Oxidative stress and abnormal glucose metabolism are the important physiological mechanisms in the occurrence and development of diabetes. Antioxidant peptides have been reported to attenuate diabetes complications by regulating levels of oxidative stress, but few studies have focused on peptides from marine bone collagen. In this study, we prepared the peptides with a molecular weight of less than 1 kD (HNCP) by enzymolysis and ultrafiltration derived fromHarpadon nehereusbone collagen. Furthermore, the effects of HNCP on blood glucose, blood lipid, liver structure and function, oxidative stress, and glucose metabolism were studied using HE staining, kit detection, and Western blotting experiment in streptozocin-induced type 1 diabetes mice. After the 240 mg/kg HNCP treatment, the levels of blood glucose, triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) in streptozotocin-induced diabetes mice decreased by 32.8%, 42.2%, and 43.2%, respectively, while the levels of serum insulin and hepatic glycogen increased by 142.0% and 96.4%, respectively. The antioxidant enzymes levels and liver function in the diabetic mice were markedly improved after HNCP intervention. In addition, the levels of nuclear factor E2-related factor 2 (Nrf2), glucokinase (GK), and phosphorylation of glycogen synthase kinase-3 (p-GSK3β) in the liver were markedly up-regulated after HNCP treatment, but the glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase1 (PEPCK1) were down-regulated. In conclusion, HNCP could attenuate oxidative stress, reduce blood glucose, and improve glycolipid metabolism in streptozocin-induced type 1 diabetes mice.Keywords:diabetes;collagen peptides;Nrf2;glucose metabolism;oxidative stress
研究领域:
药理学
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生物化学
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糖尿病研究
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DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2015
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IF 4.383
Xiaoxiao Wen,Yudong Zheng,Jian Wu,Lu-Ning Wang,Zhenya Yuan,Jiang Peng,Haoye Meng
Bacterial cellulose (BC) is an alternative nanostructured biomaterial to be utilized for a wide range of biomedical applications. Because of its low bioactivity, which restricted its practical application, collagen and collagen hydrolysate were usually composited into BC. It is necessary to develop a new method to generate covalent bonds between collagen and cellulose to improve the immobilization of collagen on BC. This study describes a facile dialdehyde BC/collagen peptide nanocomposite. BC was oxidized into dialdehyde bacterial cellulose (DBC) by regioselective oxidation, and then composited with collagen peptide (Col-p) via covalent bonds to form Schiff’s base type compounds, which was demonstrated by the results of microstructures, contact angle, Col-p content, and peptide-binding ratio. The peptide-binding ratio was further affected by the degree of oxidation, pH value, and zeta potential. In vitro desorption measurement of Col-p suggested a controlled release mechanism of the nanocomposite. Cell tests indicated that the prepared DBC/Col-p composite was bioactive and suitable for cell adhesion and attachment. This work demonstrates that the DBC/Col-p composite is a promising material for tissue engineering and regeneration.
研究领域:
生物材料学
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医学工程
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细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
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2021
·
IF 1.756
Xuqin Li,Zhou Lei,Jie Sheng,Yishan Song
In this study, a novel peptide grafted chitosan (CACS-FBP) with high peptide content, excellent moisture-absorption and moisture-retention abilities was prepared. Caffeic acid (CA) was used to modify chitosan, the highly water-soluble intermediate further reacted with fish bone collagen peptide to obtain the final product, and the synthesis of CACS-FBP was confirmed by the Fourier transform infrared spectroscopy (FT-IR), NMR, and UV-vis. The single-factor experiments indicated that the degree of substitution (DS) of CACS-FBP depended on the reaction temperature, reaction time, the mass ratio of fish bone collagen peptide to CACS (mFBP/mCACS) and the mass ratio of MTGase to CACS (mMTGase/mCACS). In addition, the antioxidant assay indicated that CACS-FBP had an excellent antioxidant capacity, and the CACS-FBP showed no cytotoxicity toward L929 mouse fibroblasts, all the results mean that the prepared peptide-containing chitosan derivative has potential application in pharmaceutical and biomedical fields.
研究领域:
生物化学
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药学
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材料科学
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产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 7
·1216
·
IF 5.1
Suxin Li,Jinhui Gu,Yiyi Liu,Weiqiang Qiu,Wenzheng Shi
Grass carp swim bladder collagen peptides (GCPs) were purified by ultrafiltration and Sephadex G-15 chromatography to obtain GCP-II, which exhibited superior antioxidant activity. GCP-II exhibited 78.22% ABTS+scavenging activity and 72.91% Fe2⁺ chelating activity, along with around 90% thermal stability between 4 °C and 100 °C. Environmental factors such as 4% NaCl reduced superoxide scavenging to 59.16% of the original and 0.2% citric acid reduced it to 71.57% of the original, while glucose showed minimal impact on the antioxidant activity of GCP-II. From 464 GCP-II sequences analyzed via LC-MS/MS, 7 bioactive peptides were selected based on antioxidant activity and functional sequence motifs, and were named peptides 1 to 7, respectively. These peptides were synthesized through solid-phase methods, validated for purity exceeding 95% using HPLC and mass spectrometry, and tested for antioxidant performance. Peptides 1, 3, 6, and 7 demonstrated notable antioxidant efficacy: peptide 6 showed 63.31% ABTS+scavenging activity at 1 mg/mL, while peptides 3 and 6 exhibited synergistic effects in DPPH and hydroxyl radical scavenging experiments, surpassing theoretical values by 0.88% and 2.16%, respectively. This study underscores the potential of synthetic GCPs and GCP-II-derived peptides as functional antioxidants, particularly peptide 3 and peptide 6.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
POLYMER TESTING
·
2022
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IF 4.931
Chao Zhang,Bowei Du,Zihao Song,Guiya Deng,Yan Shi,Tianyu Li,Yaqin Huang
Antioxidant peptides from natural polymer collagen are highly desirable and their ability to coordinate with minerals will provide new leads for the prevention and treatment of oxidative stress-related diseases. Here, we tested the changes of structures and functional properties of bovine bone collagen peptides (BBCP) after magnesium chelation. The Fourier transform infrared (FTIR) results revealed that Mg(II) chelated with BBCP mainly via amino nitrogen and carboxyl oxygen atoms. The fluorescence and circular dichroism (CD) results indicated that Mg(II) could induce and stabilize the conformational structures, and change the amino acid distribution on the surfaces of BBCP. The chelation of BBCP and Mg(II) led to the improvement of radical scavenging activities, and enhanced the intracellular antioxidant enzyme activities and GSH levels, thus demonstrating higher protective and reparative effects against H 2 O 2 -induced oxidative damage. This work provides mechanistic insights for further development of peptide-magnesium chelates for oxidative stress mitigation.
研究领域:
生物化学
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药学
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营养学
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产品关联
DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 46
·27032-27041
·
IF 3.049
Ding Ding,Bowei Du,Chao Zhang,Fakhar Zaman,Yaqin Huang
To date, many researchers have developed active components that are derived from seafood processing for the purposes of healthcare. Here, an antioxidant collagen peptide was obtained from skipjack tuna (Katsuwonus pelamis) bone by using a combination of trypsin and chymotrypsin as the catalyst. The amino acid sequence of the peptide was identified as Ser–Ser–Gly–Pro–Pro–Val–Pro–Gly–Pro–Met–Gly–Pro–Met–Gly–Pro–Arg (SSGPPVPGPMGPMGPR) by liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS) analysis. We found that the as-prepared collagen peptide can efficiently scavenge DPPH radical (IC50 3.149 mM), superoxide anion radical (IC50 3.803 mM) and ABTS radical (IC50 9.489 mM). In addition, it has been found that the methionine (Met) residue in the collagen peptide could provide a precise active site during the scavenging of DPPH radicals by Fourier transform infrared spectroscopy (FTIR) analysis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis. These results suggest that the peptide can find wide uses in the food, cosmetic and pharmaceutical industries.
研究领域:
食品科学
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生物化学
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海洋生物资源利用
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产品关联
DOI
PubMed
高影响因子
Polymers
·
2024
·Vol. 16
·期 11
·1602
·
IF 4.7
Jingyi Hu,Xiaojie Chen,Jianmin Jiang,Renbiao Mai,Han Wang,Qiming Xu,Ting Zhang
As the global facial mask market continues to grow, consumers have put forward higher requirements for the functionality and ingredients of mask products. Ordinary facial masks mostly use ordinary non-woven fabrics as the mask base fabric and are used with essence. Preservatives are generally added. At the same time, they are susceptible to the influence of the external environment and are easily oxidized, causing the mask to deteriorate and cause skin allergic reactions. In addition, traditional facial masks have problems such as poor fit with the skin, poor breathability, insufficient absorption of nutrient solutions, and easy dripping. The high specific surface area and high porosity of a nanofiber mask prepared by electrospinning technology are beneficial to the skin’s absorption of nutrients, and it has good fit with the skin and strong breathability. A unique advantage of this nanofiber mask is that it uses spray. After the mask is sprayed with water or essence, the water-soluble polymer within it can be quickly dissolved, saving a lot of time. Nanofiber facial mask products can effectively solve consumer pain points and are conducive to the high-end development of facial masks. Therefore, this article combines needleless electrospinning technology to develop a new solid-state, preservative-free, quick-dissolving nanofiber facial mask that can be prepared on a large scale. Based on needleless electrospinning technology, this article deeply explores the process parameters and their influencing mechanisms for preparing nanofiber, quick-dissolving facial masks to achieve the stable preparation of nanofiber facial masks with the best morphology; a comprehensive analysis of the structure and influence of nanofiber facial masks from micro and macro perspectives demonstrates their performance and allows evaluation of them. The experimental results show that the mask morphology is optimal under the process conditions of using a spinning liquid of 20% collagen peptide solution, a spinning voltage of 30 kV, a collection distance of 19 cm, and a liquid supply speed of 130 mL/h.
研究领域:
材料科学
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生物医学工程
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化妆品科学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
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2020
·
IF 3.701
Fengfeng Mei,Zhouwei Duan,Muxue Chen,Jinfeng Lu,Meihui Zhao,Laihao Li,Xuanri Shen,Guanghua Xia,Shengjun Chen
In this study, a total of 24 male Sprague Dawley rats were randomly divided into 3 group (Collagen peptide of Salmon salar skin group, Ss- SCP ; Collagen peptide of Tilapia nilotica skin group, Tn -SCP and Model control group, MC) to investigate the impact of a high-collagen peptide diet on the gut microbiota and host health. After 16 days intervention, the body weights of the Ss -SCP and Tn -SCP intervention groups were significantly increased and the liver index was also remarkably higher than that of the MC group. The acetic acid and propionic acid levels in feces were both significantly increased in the diet high-collagen peptide groups and valerate acid level was lower than that in the MC group. With the intervention of a high-dose collagen peptide diet, the gut microbiota of the groups was shifted with increased abundance of Lactobacillus , Unidentified-Prevotellaceae , Allobaculum, and Parasutterella , whereas the Tn -SCP administration have caused low abundance of Anaerostipes, Blautia, and Fusicatenibacter . The relative abundance of Allobaculum as well as Parasutterella was positively correlated with propionic acid and acetic acid levels, respectively. In addition, Allobaculum abundance was negatively correlated with valerate acid level. The serum valerate acid content was potentially harmful to rat health and significantly increased in the groups intervened with collagen peptide. All together, these results showed that administration of diet high-collagen peptide shifts the gut microbiota in rats and induced a disturbance in short-chain fatty acid metabolism which is potentially harmful to health.
研究领域:
营养学
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微生物学
·
胃肠病学
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·
IF 4.1
Bowei Du,Chao Zhang,Guiya Deng,Shuai Zhang,Shiqi Wang,Yuepeng Guan,Yaqin Huang
Background Nowadays, the prevalence of oxidative stress-related chronic diseases is increasing. The identification of novel antioxidant collagen-peptides to counteract oxidative stress for individuals’ health have gained significant intention. Results In this study, the collagen-peptides with antioxidant activities were separated and identified by ion chromatography (IEC), reversed-phase high-performance liquid chromatography (RP-HPLC), and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The identified antioxidant collagen-peptides were further screened by molecular docking for Keap1-targeted peptide inhibitors and their theoretical interaction mechanisms were investigated. Four novel antioxidant collagen-peptides, GPAGPIGPVG, GPAGPpGPIG, ISGPpGPpGPA and IDGRPGPIGPA with high binding affinity to Keap1 were selected. Molecular docking results demonstrated that the putative antioxidant mechanism of four antioxidant collagen-peptides contributed to their blockage of Keap1-Nrf2 interactions. The results of antioxidant activity of four antioxidant collagen-peptides proved that IDGRPGPIGPA exerted a high scavenging capacity to DPPH and ABTS free radicals, while GPAGPpGPIG improved the resistance of cells to hydrogen peroxide (H 2 O 2 ) induced oxidative damage by promoting the activation of intracellular antioxidant enzymes and the production of reduced glutathione in human hepatoma (HepG2) cells. Conclusion The antioxidant collagen-peptides (GPAGPIGPVG, GPAGPpGPIG, ISGPpGPpGPA and IDGRPGPIGPA) will be developed as the novel functional food for human health in the near future. This article is protected by copyright. All rights reserved.
研究领域:
药理学
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生物化学
·
分子生物学
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细胞生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Qianqian Ye,Kai Chen,Xiaocui Yang,Kaijun Xiao,Yi Shen
Although collagen peptides have been proved to possess wide applications in functional foods, cosmetics, medical materials and pharmaceuticals, the production of collagen peptides are deeply affected by proteases and substrate. In this study, the scalable-synthesis sulfonated polystyrene (SPS) nanospheres were utilized as accessible supports for efficient subtilisin immobilization. Detailed characterizations through SEM-EDS, TEM, TGA and FT-IR confirmed the undamaged formation of the SPS-subtilisin. Owing to the moderate hydrophobic effect and electrostatic interaction, the SPS-subtilisin could achieve 397.15 mg/g enzyme loading and 77.3% activity recovery. The tilapia skin collagen, as a resource-rich raw material, was hydrolyzed by the prepared immobilized subtilisin. The antioxidant activity of the attained peptides was verified. With the mass spectrometry and molecular docking analysis of product peptides sequences, representative peptides were synthesized and their anti-oxidation capacity and mechanism were affirmed, which further verified the undiminished catalytic ability of immobilized subtilisin.
研究领域:
生物化学
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食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Xia Li,Nengkai Cen,Lu Liu,Yongle Chen,Xi Yang,Kang Yu,Junling Guo,Xuepin Liao,Bi Shi
Efficient production of bioethanol is desirable for bioenergy large-scale applications, but it is severely challenged by ethanol and sugar stresses. Here, collagen peptide (CP), as a renewable nitrogen-containing biomass, remarkably enhanced the stress resistance of Saccharomyces cerevisiae SLL-510 against ethanol challenge, based on its unique amino acid composition. Transcriptome analysis showed that the energy, lipid, cofactor, and vitamin metabolism may involve in stress tolerance provided by CP. When CP was added into the media containing 249.99 mg/mL glucose, the bioethanol yield increased from 8.03 to 12.25% (v/v) and 11.35 to 12.29% (v/v) at 43 and 120 h, respectively. Moreover, at 286.79 mg/mL glucose, the highest yield reached 14.48% (v/v), with 99.58% glucose utilization rate. The protection and promotion effects of CP were also shown by four other industrial S. cerevisiae strains. These results coupled with the advantages of abundant reserves, cleanliness, and renewability revealed that CP is a promising economically viable and industrially scalable enhancer for bioethanol fermentation.
研究领域:
生物技术
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微生物学
·
可再生能源
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）