ANALYTICA CHIMICA ACTA
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2026
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IF 6.1
Yali Yang,Hui Ding,Kaijun Quan,Hongwei Zhao,Mingxia Sun,Zhimin Yang,Xiuhui Liu,Hongdeng Qiu,Jia Chen
Background To address the limitations of traditional single-mode stationary phases in separating complex samples, mixed-mode or multi-mode chromatographic stationary phases, which can provide two or more interaction mechanisms, have attracted extensive research interest. Among them, sulfonated pyrene derivatives present a promising candidate ligand, as they synergistically retain the hydrophobicity and π-π interaction capability of the pyrene moiety along with the hydrophilicity and strong cation-exchange capacity provided by the sulfonic acid groups. This synergistic multi-mechanism design significantly enhances separation selectivity for diverse and complex components. Results A pyrene trisulfonic acid-functionalized silica stationary phase (Sil-PyTSa) was successfully prepared and evaluated. In hydrophilic interaction liquid chromatography (HILIC) mode, Sil-PyTSa could efficiently separate nucleobases and nucleosides, ginsenosides, and alkaloids. For nucleobases and nucleosides, it utilized hydrophilic, electrostatic, and π-π interactions, showing superior retention and selectivity over a control HILIC column. In reversed-phase liquid chromatography (RPLC) mode, favorable separation of PAHs was achieved via hydrophobic and π-π interactions, enabling faster analysis and lower solvent consumption than a C18 column. In ion-exchange chromatography (IEC) mode, baseline separation of 14 rare earth elements (REEs) and removal of Fe/U impurities were accomplished. The column efficiency reached 95,920 N/m for naphthalene. Additionally, Sil-PyTSa achieved effective separation for REEs and iron (Fe) in a simulated NdFeB leaching solution and detected nucleobases and nucleosides in Angelica, indicting promising potential for analyzing complex matrices. Significance and novelty This work developed a novel multi-mode chromatographic stationary phase with multiple interactions mechanisms to enable high-performance separations in HILIC, RPLC, and IEC modes. It demonstrates good separation efficiency and selectivity, low solvent consumption, and high stability. This offers a versatile and green solution for the separation of complex samples with diverse analytes.
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PROGRESS IN ORGANIC COATINGS
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2026
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IF 7.9
Peiyu Su,Dingjia Liu,Licheng Liu,Juanjuan Wang,Chuanhui Yang,Shoubiao Ma,Yingfeng Yu
Temporary bonding adhesives that enable damage-free fixation and on-demand debonding are critical for precision manufacturing processes such as semiconductor, OLED, and PCB/FPC fabrication. This study developed a 254 nm UV-responsive pressure-sensitive adhesive (PSA) based on thymine photodimerization for efficient and residue-free debonding. By integrating the thymine moiety into an acrylic PSA system with butyl acrylate (BA), methyl methacrylate (MMA), and acrylic acid copolymers, 254 nm UV light exposure triggers adhesion loss through photodimerization-induced crosslinking. As a result, the peel strength decreases significantly from 11.22 to 3.16 N/25 mm. Rheological analysis reveals a distinct debonding mechanism, where the reduction in adhesion is predominantly governed by a decrease in high-frequency loss modulus (G"), associated with the disruption of hydrogen-bonded networks, rather than conventional extensive crosslinking-induced dewetting. In addition, spatially controlled debonding is demonstrated via patterned UV irradiation. This work provides new insights into the design of photo-switchable PSAs, offering a promising strategy for high-precision temporary bonding applications in advanced manufacturing.
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INORGANIC CHEMISTRY
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2025
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IF 4.7
Tao Cheng,Lu Liu,Jia Hui Chen,Pan Wang,Jian Ming Pan
Metal–organic frameworks (MOFs) exhibit a diverse array of applications in the fields of adsorption, catalysis, sensing, and separation. However, the limited water stability of MOFs poses challenges in the separation domain, particularly due to the structural similarities among water-soluble nucleoside compounds, which complicate the selective adsorptive separation of these compounds from complex mixtures. In this study, hydrophilic flower-like zirconium-based MOF nanosheets (Zr-FNSs) were prepared by a one-step hydrothermal method. The investigation focused on their adsorption capacity and the mechanism of action, with respect to various nucleosides. Adsorption experiments were conducted under varying pH conditions to identify the optimal pH and to assess the influence of phosphate number on the adsorption of adenosine compounds. The kinetic adsorption capacities of Zr-FNSs for adenosine monophosphate (AMP), adenosine diphosphate (ADP), and adenosine triphosphate (ATP) were determined to be 254.81, 211.35, and 182.97 μmol g–1, respectively, demonstrating a gradient decrease that may be attributed to increased spatial resistance or a limited number of available adsorption sites. Zr-FNSs have excellent regenerative properties. This work underscores the significance of functional group interactions in the selective adsorption of nucleoside and provides valuable insights for the design of MOF-based adsorbents for the selective separation and purification of nucleoside.
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JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Minyi Yuan,Zheng Zhao,Ji Wu,Sumin Bian,Yuling Liao,Ying Zhou,Huikai Shao,Zhengjin Jiang,Qiqin Wang
To meet the increasing demand for the separation of complex samples, a novel monolithic stationary phase with hydrophobic and hydrophilic functionalities was developed by using 3-(1-(4-vinylbenzyl)-1H-imidazol-3-ium) propane-1-sulfonate (VBIPS) as monomer, pentaerythritol triacrylate (PETA) as crosslinker. The optimized mixed-mode poly(VBIPS- co -PETA) monolith exhibited good mechanical strength, high permeability (K = 0.23 × 10 -14 m 2 in 100% ACN, 0.23 × 10 -14 m 2 in 100% MeOH, and 0.11 × 10 -14 m 2 in 100% H 2 O), and multiple interaction modes, including hydrophilic, hydrophobic, hydrogen, and electrostatic interactions. The highest column efficiency of 83,330 plates/m was achieved for thiourea at a linear velocity of 0.23 mm/s. Systematic evaluations confirmed strong retention and separation of polar substances (e.g., benzoic acids, nucleoside bases, phenols), primarily due to the sulfobetaine-derived hydrophilicity as well as effective separation of nonpolar substances (e.g., aniline, aryl ketones, polyaromatic hydroxy compounds), facilitated by π-π stacking interactions between analytes and benzene rings within the stationary phase. Overall, this zwitterionic imidazolium sulfonate-functionalized monolith successfully combines hydrophobic and hydrophilic groups in a single continuous bed, enabling efficient separation of both polar and nonpolar analytes under low backpressure. This work enriches the library of mixed-mode stationary phases, and broadens the detection scope for complex sample analysis.
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JOURNAL OF HAZARDOUS MATERIALS
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2026
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IF 10.6
Xinyi Tang,Yan Wang,Xiumei Chen,Wei Kong,Xue He,Yi Yang
Waterborne pathogenic microorganisms pose serious public health threats by causing gastrointestinal infections and disease outbreaks. However, conventional disinfection technologies suffer from limitations such as high equipment costs, the formation of chlorinated byproducts, and limited scalability. To address these challenges, we developed a heterogeneous oxidative system based on nitrogen-doped carbon materials (N-Cs) for peroxymonosulfate (PMS) activation and efficient microbial inactivation. Using Escherichia coli ( E. coli) as a model pathogen, we demonstrate that the N-Cs /PMS system achieves rapid and effective disinfection via a non-radical, synergistic mechanism. Singlet oxygen ( 1 O 2 ) is identified as the dominant reactive species, initiating a cascade of cellular damage including membrane dysfunction, genomic DNA degradation, and the induction of antioxidant enzymes (e.g., SOD, CAT). In addition, transcriptomic analysis reveals that 1 O 2 generated in the system perturb gene expression related to metabolic regulation and catalytic activity, thereby disrupting bacterial physiology at a systemic level. This work highlights the critical role of 1 O 2 in non-radical disinfection and provides mechanistic insights into PMS-based advanced oxidation processes for microbial control in water treatment applications.
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MICROCHEMICAL JOURNAL
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2026
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IF 5.2
Tao Liu,Dan Li,Wenying Hu,Amirah F. Kamaruddin,Zhisong Lu,Wei Sun
Reliable electrochemical determination of adenine (Ade) in biological samples remains challenging due to interference from structurally similar nucleobases, nonspecific adsorption, and instability of conventional sensing interfaces, which compromise selectivity and signal reproducibility. Here, we report an interface engineered electrochemical molecularly imprinted sensor based on disposable screen-printed electrodes (SPEs). The sensing platform integrates a poly( o -phenylenediamine) molecularly imprinted recognition layer with Au nanoparticles/MXene quantum dots/nitrogen-doped graphene (AuNPs/MQDs/NGR) nanocomposite transduction interface. MXene quantum dots (MQDs) provide abundant edge and terminal sites that promote uniform AuNPs nucleation and anchoring on NGR, forming a conductive and structurally stable sensing layer with reduced charge-transfer resistance. This engineered interface enables stable electropolymerization of the imprinted film and improves signal stability and electrode-to-electrode reproducibility. The sensor exhibited a linear response for Ade from 0.25 to 80.0 μM (R 2 = 0.992) with a detection limit of 0.094 μM and recoveries of 96.40–103.84% in human serum sample. This strategy provides a reproducible and selective electrochemical platform that addresses key limitations of current adenine sensors for analysis in complex biological matrices.
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ANALYTICA CHIMICA ACTA
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2026
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IF 6.1
Haiyan Cao,Bin Yang,Wenfei Dong,Wenbing Shi
Background The accurate detection of 2,3,5,6-tetramethylpyrazine (TMP) in complex matrices is significant for food quality control and biomedical analysis. Despite this need, a dedicated optical sensing platform for TMP has not been reported to date. Results Here, we present a novel ratiometric fluorescent probe based on a Tb–EDTA complex, which introduces a sensing strategy founded on coordination-induced modulation of the ligand-to-metal charge transfer (LMCT) process. Specific coordination of TMP's pyrazine nitrogen atoms to the Tb 3+ center results in a characteristic ratiometric response: quenching of the emission at 548 nm and concomitant enhancement at 650 nm. The probe exhibits excellent analytical performance with a linear range of 0.1―40 μM, a detection limit of 0.038 μM, and high selectivity over structurally similar interferents. Its practical applicability is demonstrated by reliable quantification of TMP in real samples, including sauce-flavor Baijiu, sake, and human urine, with recoveries of 94.54–104.82% and relative standard deviations below 10.57%. Significance This work establishes a pioneering optical sensing approach for TMP detection, providing a valuable analytical tool for flavor assessment in alcoholic beverages and pharmacological monitoring in biological systems.
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JOURNAL OF CHROMATOGRAPHY A
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2023
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IF 4.1
Li Gao,Yuan Wang,Yaqiong Qin,Yaming Sun,Lijun He,Shusheng Zhang,Wenjie Zhao
Carbohydrate is a renewable, sustainable, hydrophilic, and biodegradable natural product, which is widely used in the field of adsorption. In this study, a glucose-based covalent organic polymer (COP) coated silica was fabricated by facile solvent knitting reaction between tetrabenzylglucose and silica-phenyl with anhydrous aluminum trichloride as catalyst, forming a core-shell stationary phase (donated as SiO 2 @COP Bn-glu ) for high performance liquid chromatography. The prepared SiO 2 @COP Bn-glu was characterized by scanning electron microscopy, transmission electron microscopy, Fourier-transform infrared spectrometry, solid-state 13 C nuclear magnetic resonance spectrometry, X-ray photoelectron spectroscopy, and N 2 adsorption-desorption experiments. Owing to the coexistence of benzene units and alkyl, hydroxyl and ether groups in the skeleton of COP Bn-glu shell, the developed chromatographic packing exhibited reversed-phase/hydrophilic interaction mixed-mode with multiple retention mechanisms, such as hydrophobic, π-π, hydrogen bonding, and electron donor-acceptor interactions. The results revealed that the SiO 2 @COP Bn-glu column demonstrated excellent selectivity and retention behavior for both hydrophilic and hydrophobic compounds with good repeatability and stability. Meanwhile, the chromatographic performance of the prepared SiO 2 @COP Bn-glu column was compared with a C 18 column to assess the role of the coating COP Bn-glu shell. Therefore, the development of the SiO 2 @COP Bn-glu stationary phase expands the potential application of COPs in separation field.
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AIP Advances
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2023
·Vol. 13
·期 3
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IF 1.6
Wang Qiaolin,Xu Lihe,Qin Zhengbo,Yang Xinyan,Zheng Xianfeng
Deoxyribonucleic acid (DNA) carries the genetic information necessary for the synthesis of RNA and proteins; it is a biological macromolecule essential for the development and proper functioning of living organisms and is composed of nucleobases, deoxyribose, and phosphate. The four nucleobases in DNA are adenine (AD), guanine (GU), thymine (TY), and cytosine (CY). Abnormal concentrations of these four nucleobases in an organism have a significant impact on disease diagnosis. Therefore, the qualitative and quantitative detection of these DNA nucleobases in organisms is helpful to diagnose certain diseases. In this work, we report the simultaneous determination of purine (AD, GU) and pyrimidine (TY, CY) nucleobases in DNA using laser-induced acoustic desorption (LIAD) with electron ionization (EI)/time-of-flight mass spectrometry (TOFMS). The purine (MW 120 Da) samples were used as model compounds to assess the sensitivity and quantitative performance of the instrument. Its limits of detection assessed using the LIAD/EI/MS method were ∼0.5–1.2 pg under optimal conditions, and their calibration curves exhibited good linearity (R 2 = 0.98). The LIAD/TOFMS was successfully applied in the simultaneous detection of AD, GU, TY, and CY in real DNA samples. The advantage of this technique is simple, fast, and without complex pre-treatment processes. In addition, a quartz-enhanced LIAD (QE-LIAD) source was used to improve the signal strength. The desorption for complex biomolecules shows that the QE-LIAD is still a “gentle” desorption source.
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Materials Today Communications
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2023
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IF 3.8
Ran Feng,Jiazhi Meng,Hualei Yuan,Xu Zhang,Chunlang Gao,Chaogang Ban,Yizhong Guo,Kaiwen Wang
Cancers, Down’s syndrome, Alzheimer’s and other diseases of gene mutation always occur with anomalous changes of DNA nucleic acid base. Therefore, the development of highly sensitive and reproducible nucleic acid sensors that based upon surface enhanced Raman scattering (SERS) is of immense interest for clinical medical analysis and diagnosis. Herein, a rationally designed core-shell assembly of Au nanoparticle wrapped with Au doped Bi 5 O 7 Br nanotube with eliminated fluorescent background and notable chemical enhancement effect was exploited and served as a SERS sensor for detection of adenine. Especially, we found the fluorescent background was remarkably suppressed in Raman spectra by introducing Au element into Bi 5 O 7 Br. The sensor exhibited a maximum SERS enhancement factor of up to 5.45 × 10 7 and a detection limit as low as 10 −11 M after constructing Au nanoparticle/Au atom doped Bi 5 O 7 Br heterojunction . More importantly, good reproducibility and long term stability under work condition were achieved. To gain in-depth understanding into chemical mechanism (CM) of the Raman signal enhancement, density functional calculations (DFTs) were combined with atom-specific model derived via aberration corrected transmission electron microscope (AC-TEM) to demonstrate the enhanced charge transfer (CT) and substrate-adsorbate adsorption. The doping and coupling strategy holds the potential to fabricate high signal-to-noise ratio SERS sensors for other molecule detection.
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Frontiers in Pharmacology
·
2023
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IF 5.6
Lvyuan Fan,Zhiyu Pan,Xu Liao,Yilin Zhong,Juan Guo,Rui Pang,Xinhai Chen,Guozhu Ye,Yubin Su
Background: Methicillin-resistant Staphylococcus aureus (MRSA) has now become a major nosocomial pathogen bacteria and resistant to many antibiotics. Therefore, Development of novel approaches to combat the disease is especially important. The present study aimed to provide a novel approach involving the use of nucleotide-mediated metabolic reprogramming to tackle intractable methicillin-resistant S. aureus (MRSA) infections. Objective: This study aims to explore the bacterial effects and mechanism of uracil and gentamicin in S. aureus. Methods: Antibiotic bactericidal assays was used to determine the synergistic bactericidal effect of uracil and gentamicin. How did uracil regulate bacterial metabolism including the tricarboxylic acid (TCA) cycle by GC-MS-based metabolomics. Next, genes and activity of key enzymes in the TCA cycle, PMF, and intracellular aminoglycosides were measured. Finally, bacterial respiration, reactive oxygen species (ROS), and ATP levels were also assayed in this study. Results: In the present study, we found that uracil could synergize with aminoglycosides to kill MRSA (USA300) by 400-fold. Reprogramming metabolomics displayed uracil reprogrammed bacterial metabolism, especially enhanced the TCA cycle to elevate NADH production and proton motive force, thereby promoting the uptake of antibiotics. Furthermore, uracil increased cellular respiration and ATP production, resulting the generation of ROS. Thus, the combined activity of uracil and antibiotics induced bacterial death. Inhibition of the TCA cycle or ROS production could attenuate bactericidal efficiency. Moreover, uracil exhibited bactericidal activity in cooperation with aminoglycosides against other pathogenic bacteria. In a mouse mode of MRSA infection, the combination of gentamicin and uracil increased the survival rate of infected mice. Conclusion: Our results suggest that uracil enhances the activity of bactericidal antibiotics to kill Gram-positive bacteria by modulating bacterial metabolism.
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JOURNAL OF CHROMATOGRAPHY A
·
2023
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IF 4.1
Tong Chen,Shuya Wang,Xufang Zong,Bingxiang Li,Ye Shu,Xinyuan Di,Wanning Zhu,Guangsan Song,Jun Jiang
Periodic mesoporous organosilicas (PMO) hydrophilic microspheres were synthesized by co-condensation of sulfated polysaccharide from Lilum lancifolium Thunb. bridged silane (SLLTPBS) and polyhedral oligomeric silsesquioxane (POSS) as stationary phase (PMO(SLLTP-POSS)) for per aqueous liquid chromatography (PALC), which would overcome the disadvantages of using a large amount of acetonitrile on the hydrophilic interaction liquid chromatography (HILIC) columns. Average particle size of PMO (SLLTP-POSS) microspheres was 4.9 μm, which was suitable for stationary phase. The retention mechanism of the stationary phase in PALC was mainly hydrophobic interactions and also included some ion-exchange interactions and electrostatic interactions. The acid-base resistance was greatly improved compared to the C18 column. The PMO(SLLTP-POSS) column under PALC mode had increased the resolution when separating some hydrophilic compounds such as eight organic acids and eleven sweeteners compared with the C18 column and HILIC column. The new column was more efficient than the HILIC columns. Additionally, a PALC-triple quadrupole mass spectrometry approach for the simultaneous identification of the eleven sweeteners was developed. The averagere coveries of the eleven compounds were 70.20%–91.33% with the relative standard deviation (RSD) range of 1.74% to 4.27%. The results showed good precision and accuracy of the method.
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危险品化学品经营许可证（带存储）