Food Bioscience
·
2025
·
IF 5.9
Yujing Zhou,Jue Xu,Xiankang Fan,Zihang Shi,Wei Wang,Ming Huang,Kaiyong Yao,Hongbing Yan,Changyu Zhou,Yangyang Hu,Daodong Pan
This research sought to find a novel way to control the biogenic amines (BAs) in air-dried goose through pulsed electric field (PEF) combined with mixed bacteria fermentation. The findings indicated that Lactiplantibacillus plantarum ( L . plantarum ) PDD-1 and Lactococcus lactis subsp. lactis ( L . lactis ) JCM5805 exhibited superior monoamine oxidase (MAO) and diamine oxidase (DAO) activities and robust degradation abilities for the four BAs (putrescine, cadaverine, histamine, tyramine) across various environmental conditions (temperature, NaCl concentration, and initial pH). Subsequent investigations revealed that the mixed bacteria ( L . plantarum PDD-1: L . lactis JCM5805 at a 1:1 ratio) demonstrated a more pronounced effect on BAs reduction compared to individual starter culture, particularly for putrescine and tyramine. The study results indicated that the optimal conditions for reducing BAs in air-dried goose was an electric field strength of 0.7 kV/cm applied for 150 s, followed by air-drying for 4 d. Consequently, the total BA content in the air-dried goose decreased from 15.98 ± 1.27 mg/100 g to 7.54 ± 0.04 mg/100 g. Targeted amino acid metabolomics further defined the degradation mechanism. This study is instructive for low BAs meat processing methods.
研究领域:
食品科学
·
微生物学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Huanhuan Jin,Ziyuan Wang,Zhongji Pu,Haoran Yu,Yuhuan Chen,Xiaoshu Zhou,Lirong Yang,Shuyun Ju,Jianping Wu
Optically pure 1,2,3,4-tetrahydroquinolines (THQs) represent a class of important motifs in many natural products and pharmaceutical agents. While recent advances on redox biocatalysis have demonstrated the great potential of amine oxidases , all the transformations focused on 2-substituted THQs. The corresponding biocatalytic method for the preparation of chiral 4-substituted THQs is still challenging due to the poor activity and stereoselectivity of the available enzyme . Herein, we developed a biocatalytic kinetic resolution approach for enantiodivergent synthesis of 4-phenyl- or alkyl-substituted THQs. Through structure-guided protein engineering of cyclohexylamine oxidase derived from Brevibacterium oxidans IH-35 A (CHAO), the variant of CHAO (Y215H/Y214S) displayed improved specific activity toward model substrate 4-phenyl substituted THQ (0.14 U/mg, 13-fold higher than wild-type CHAO) with superior ( R )-stereoselectivity ( E > 200). Molecular dynamics simulations show that CHAO Y215H/Y214S allows a suitable substrate positioning in the expanded binding pocket to be facilely accessed, enabling enhanced activity and stereoselectivity . Furthermore, a series of 4-alkyl-substituted THQs can be transformed by CHAO Y215H/Y214S, affording R -isomers with good yields (up to 50 %) and excellent enantioselectivity (up to ee > 99 %). Interestingly, the monoamine oxidase from Pseudomonas fluorescens Pf0–1 ( Pf MAO1) with opposite enantioselectivity was also mined. Together, this system enriches the kinetic resolution methods for the synthesis of chiral THQs.
研究领域:
生物催化
·
有机化学
·
蛋白质工程
·
药物科学
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DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2017
·
IF 9.446
Zhan Wang,Xianjun Lang
Herein, we report an efficient protocol for the selective oxidation of amines with the sole involvement of conduction band electrons (e cb − ) of TiO 2 for both the activation of O 2 and the ensuing oxidation reaction. Visible light was harvested by dye molecules on the surface of TiO 2 , which injects electrons into its conduction band, generating dye radical cations. Charge transfers from dye radical cations to amines are relayed by the catalytic cycle of (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO) to TEMPO + , which is an oxidant that can conduct the direct two-electron oxidation of amines to imines. TEMPO was regenerated by the oxidation of TEMPOH with superoxide generated by the interaction of e cb − and O 2 . This work suggests that one can exploit the strategy of cooperative photocatalysis with each component performing in its optimal role: TiO 2 for O 2 activation, dye for visible light harvesting, and TEMPO for oxidative transformations.
研究领域:
化学
·
材料科学
·
催化反应
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产品关联
DOI
高影响因子
Catalysis Science & Technology
·
2019
·Vol. 10
·期 2
·440-449
·
IF 5.726
Pengqi Zhu,Jin Zhang,Jie Wang,Peng Kong,Yunwei Wang,Zhanfeng Zheng
Hydroxyl coordinated ruthenium dispersed on the surface of γ-Al2O3 can be applied to the selective oxidation of amines with light irradiation and an atmospheric pressure of O2 at room temperature. Sunlight is also an effective light source for the selective aerobic oxidation of primary amines to corresponding nitriles. The high photocatalytic activity and selectivity over Ru/γ-Al2O3 originate from the adsorption of amines and imine intermediates on the abundant surface OH groups of the photocatalyst and further formation of Ru–amide species by ligand exchange of adsorbed amines and imine intermediates with adjacent exposed active Ru sites. Light is introduced to the system successfully via the formation of Ru–amide species, which are used as the light absorption sites of the photocatalytic selective oxidation of amines. Primary amines are directly converted to corresponding nitriles via a two-step oxidative dehydrogenation process.
研究领域:
化学工程
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材料科学
·
环境科学
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产品关联
DOI
高影响因子
JOURNAL OF CATALYSIS
·
2025
·
IF 6.5
Huacheng Ying,Zeyu Wen,Jia Yao,Mowei Zhou,Yongtao Wang,Haoran Li
Constructing N−O bond in amines via aerobic oxidation is of high interest for producing cyclohexanone oxime; however, the mechanism remains disputed. Concerning TiO 2 /NHPI co-catalyzed aerobic oxidation of cyclohexylamine, here a convincing mechanism involving α-C radical and oxaziridine as key intermediates is demonstrated. All the evidences from electron paramagnetic resonance, mass spectrometry, and product analysis with methylated substrates lead to the same story about the mechanism, where both radical translocation of cyclohexylamino radical and epoxidation of 1-hydroperoxycyclohexylamine are mediated by double proton transfer and are the key to convert amine into oxime. Near IR analysis indicate that surface hydroxyl groups on TiO 2 facilitate the double proton transfer process. Here the unveiled role of surface hydroxyl on TiO 2 highlights its potential for broader applications in amine activation. Additionally, the well-established radical mechanism provides valuable insight into the construction of N−O bonds in amines.
研究领域:
化学
·
催化反应
·
材料科学
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产品关联
DOI
高影响因子
INORGANIC CHEMISTRY
·
2022
·
IF 5.436
Zhi Hua Zhang,Li Xia Chen,Yun Qian Zhang,Qian Jiang Zhu,Kai Chen,Zhu Tao
The anion-induced outer surface interaction of Q[n]s is an important driving force in the construction of Q[n]-based supramolecular frameworks. In this work, a symmetric tetramethyl-substituted cucurbit[6]uril (TMeQ[6]) is selected as the basic structural block. Using the anion-induced outer surface interaction of Q[n]s derived from [CdxCly]n− anions formed by Cd2+ cations in a HCl medium, four different TMeQ[6]-[CdxCly]n−-based supramolecular frameworks are constructed. Three of the most common TMeQ[6]-[CdxCly]n−-based supramolecular frameworks are selected for further vulcanization, and three different CdS/TMeQ[6]-based framework catalysts with different structures and properties are obtained. The catalytic activities of these three CdS/TMeQ[6]-based framework catalysts are investigated by the coupling photocatalytic reaction of aminobenzyl, and the results showed that the catalytic activities of the three catalysts are all higher than that of pure CdS. Therefore, this work establishes that it is possible to establish a method for synthesizing the Q[n]-based framework-supported catalysts by first synthesizing TMeQ[6]-[CdxCly]n−-based supramolecular frameworks and then forming Q[n]-based framework supported catalysts by sulfurization or reduction.
研究领域:
材料科学
·
催化化学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Xiaoyun Dong,Huimin Hao,Ningning Wang,Hong Yuan,Xianjun Lang
Semiconductor photocatalysis can carry out selective chemical transformations under ambient conditions, mitigating the associated environmental consequences. However, a single semiconductor photocatalyst usually cannot perform the transformations satisfactorily from the aspects of light-absorption, efficiency, and selectivity , etc. To address these challenges, cadmium sulfide (CdS)/titanate hybrid was fabricated by simultaneously growing titanate and CdS and had been comprehensively characterized. The optimized CdS/titanate hybrid can power the highly selective oxidative homocoupling of amines under the irradiation of green light-emitting diodes (LEDs). Specifically, CdS with a narrow bandgap captures green light; the conduction band of titanate activates molecular oxygen (O 2 ). The valence band of CdS could ensure the selective oxidative homocoupling of amines in methanol (CH 3 OH). The hybridization between CdS and titanate accounts for the expeditious oxidative homocoupling of amines into imines and the improved stability. Reactive oxygen species (ROS) quenching experiments and in situ electron paramagnetic resonance (EPR) tests suggest that superoxide anion (O 2 − ) and benzylamine radical are intermediates en route to imines. This work highlights the viability of hybridization of dual semiconductor nanostructures in implementing visible light-powered selective conversions.
研究领域:
材料科学
·
环境化学
·
半导体技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Qiaoqiao Ci,Hong Yang,Yuanzhi Bian,Zheng Li,Jinhua Liu,JiFu Wei,Naidi Yang,Juhong Chen,Lin Li,Chengwu Zhang
Currently, the development of simple, fast, low-cost, and environmentally friendly MAO-A specific detection methods remains a huge challenge. Herein, a simple in situ fluorescent copolymer dots (FCDs)-based kit is constructed for the specific detection of monoamine oxidase-A in cell, tissue and human prostate cancer. The developed kit is depended on the high reaction activity between epinephrine and polyethyleneimine to form FCDs and the selective catalyzing activity of MAO-A on epinephrine. The gained FCDs are identified and characterized by high-resolution TEM, absorption and fluorescence spectrum. The present kit achieves highly sensitive detection of MAO-A with a limit of detection (LOD) of 20 ng/mL as well as screening of inhibitor with an IC50 (the concentration responding to 50% inhibition) of 1.071 μM by using clorgyline as a model. The MAO-A and MAO-B as the two isoforms of MAO are discriminated successfully, demonstrating the excellent selectivity of our kit. Noteworthy, the proposed method is applicable for measuring MAO-A not only from serum samples, but also for endogenous MAO-A in cells, mouse tissues and human prostate tumors with satisfied results. The proposed kit will be a great potential tool for human prostate cancer (PCa) diagnosis and the development of anti-PCa drug screening.
研究领域:
癌症生物学
·
分析化学
·
生物医学工程
·
药理学
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2020
·Vol. 38
·期 11
·1292-1298
·
IF 3.826
Chuanchuan Zhao,Yongjun Gao,Ziyi Zhang,Ding Ma
Summary of main observation and conclusion Sustainable phytic acid and cellulose are selected as precursors to fabricate P-doped carbon material (C-P-500), which possesses the high surface area and exhibits excellent catalytic activity and stability in the oxidative coupling of benzylamines (conversion > 95%, yield > 95%). During the carbonization of cellulose in the muffle furnace, phytic acid can efficiently prevent the cellulose-derived carbon from combustion and create micro/meso porous structure because of its special function of fire retardation. Control experiments and characterizations demonstrate that P-containing groups anchoring on C-P-500 improve the catalytic performance of carbocatalyst in the oxidation of benzylamines to imines. The excellent catalytic activity of C-P-500 should be contributed collectively by carbonyl and P=O(OH) 2 groups. Due to the sustainable property of precursors and simple methodology for fabricating P-doped carbocatalyst, along with the moderate reaction conditions, it is a green catalytic system for the oxidative coupling of benzylamines to imines.
研究领域:
化学
·
可持续化学
·
催化科学
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产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2015
·
IF 2.711
Jun Dai,Juan Yang,Xiaohan Wang,Lei Zhang,Yingjie Li
Wirelike catalysts of mixed-phase TiO 2 (B)/anatase TiO 2 , bare anatase TiO 2 and TiO 2 (B) are synthesized via calcining precursor hydrogen titanate obtained from hydrothermal process at different temperatures between 450 and 700 °C. Under visible light irradiation, mixed-phase TiO 2 (B)/anatase TiO 2 catalysts exhibit enhanced photocatalytic activity in comparison with pure TiO 2 (B) and anatase TiO 2 toward selective oxidation of benzylamines into imines and the highest photocatalytic activity is achieved by TW-550 sample consisting of 65% TiO 2 (B) and 35% anatase. The difference in photocatalytic activities of TiO 2 samples can be attributed to the different adsorption abilities resulted from their crystal structures and interfacial charge separation driven by surface-phase junctions between TiO 2 (B) and anatase TiO 2 . Moreover, the photoinduced charge transfer mechanism of surface complex is also proposed over mixed-phase TiO 2 (B)/anatase TiO 2 catalysts. Advantages of this photocatalytic system include efficient utilization of solar light, general suitability to amines, reusability and facile separation of nanowires catalysts.
研究领域:
材料科学
·
环境科学
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产品关联
DOI
高影响因子
SENSORS
·
2017
·Vol. 17
·期 3
·530
·
IF 2.677
Ru-Jia Yu,Jia-Jia Sun,Heng Song,Jing-Zhi Tian,Da-Wei Li,Yi-Tao Long
Real-time monitoring of chemical reactions is still challenging as well as important to study reaction mechanisms and reaction kinetics. Herein, we demonstrated the real-time monitoring of o-phenylenediamine (OPD) oxidation on the surface of gold nanoparticles by surface-enhanced Raman spectroscopy (SERS). The oxidation mechanism and the reaction kinetics were investigated on the basis of the SERS spectrum variation and the related density functionalized theory calculation. It was shown that the oxidation of OPD in the presence of copper ions was a two-step process of the deprotonation of the amino group on the aromatic rings and the rearrangement of the electron cloud to a π-conjugated system, which may open a new door to comprehensively understand the reaction process.Keywords:surface-enhanced Raman spectroscopy;oxidation of o-phenylenediamine;real-time monitoring
研究领域:
化学动力学
·
理论计算
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Haijing Gao,Yajie Chen,Huali Li,Fangfang Zhang,Guohui Tian
Copper-based chalcogenides have been paid intensive attention as alternate photothermal catalysts for solar energy conversion. Despite considerable progress of copper sulfides nanostructure fabrication, combining independent copper sulfides polymorphs with various stoichiometries by forming heterojunction is still challenging. Herein, unique hierarchical nanosheet-assembled Cu 7 S 4 -Cu 9 S 8 heterostructure hollow cubes with remarkable photothermal aerobic oxidation activity were prepared by a facile onepot chemical transformation route using Cu 2 O cubes as sacrificial template for the first time. In this reaction system, the introduction of proper sulfur source thioacetamide and moderate alkaline conditions plays a crucial role in phase transformation, and through tunning the reaction time, a series of Cu 7 S 4 , Cu 7 S 4 -Cu 9 S 8 , and Cu 9 S 8 can be selectively prepared. Furthermore, the Cu 7 S 4 -Cu 9 S 8 polymorphic heterostructure hollow cubes exhibit significantly enhanced photothermal aerobic oxidation performance of amines to the corresponding imines compared with the individual Cu 7 S 4 and Cu 9 S 8 in both N , N -dimethylformamide and acetonitrile solvent medium. Our results indicated that the strong near-infrared plasmonic absorption of Cu 7 S 4 -Cu 9 S 8 facilitated hot carrier transport between Cu 7 S 4 and Cu 9 S 8 , which subsequently promoted the catalytic reactions. Especially after uniform load of Au-Pt alloy nanoparticles, the photothermal aerobic oxidation activity was further enhanced due to their boosted synergistic local coupling enhancement effect between distinct components in the N , N -dimethylformamide solvent medium. Such enhanced photothermal reaction could effectively promote sunlight utilization in a wide range of photocatalytic reactions.
研究领域:
材料科学
·
化学工程
·
纳米技术
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）