TALANTA
·
2022
·
IF 6.556
Yaping Dong,Xiaojin Luo,Yiqun Liu,Cunling Yan,Haixia Li,Jicheng Lv,Li Yang,Yue Cui
In clinical practice, sera creatinine level is regarded as a crucial biomarker for the diagnosis, staging and monitoring of kidney disease. An amperometric biosensor is rapid, accurate, and cost-effective, with a portability and a simple operation. Herein, we report for the firsttime a disposable, printed amperometric biosensor for the clinical evaluation of creatinine in renal function detection. The sensor is constructed based on Prussian blue/carbon-graphite paste as the working electrode and the immobilization of creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase. The creatinine biosensor shows a linear detection range from 0.05 to 1.4 mM with a detection time of about 3 min. In addition, the sensor shows a high stability that can maintain above 86% of the initial activity after being stored for over 4 months. Moreover, the sensor shows almost the same results as those with the Jaffe method for measuring the real blood samples. We anticipate that the creatinine biosensor could be widely used in the medical and healthcare areas, especially for at-home testing and onsite medical examinations.
研究领域:
临床化学
·
生物医学工程
·
医疗器械
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yumei Hu,Longhai Dai,Yuhang Xu,Du Niu,Xuechun Yang,Zhenzhen Xie,Panpan Shen,Xian Li,Hao Li,Lilan Zhang,Jian Min,Rey-Ting Guo,Chun-Chi Chen
Ochratoxin A (OTA) contamination in various agro-products poses a serious threat to the global food safety and human health, leading to enormous economic losses. Enzyme-mediated OTA degradation is an appealing strategy, and the search for more efficient enzymes is a prerequisite for achieving this goal. Here, a novel amidohydrolase, termed Pw ADH, was demonstrated to exhibit 7.3-fold higher activity than that of the most efficient OTA-degrading ADH3 previously reported. Cryo-electron microscopy structure analysis indicated that additional hydrogen-bond interactions among OTA and the adjacent residue H163, the more compact substrate-binding pocket, and the wider entry to the substrate-access cavity might account for the more efficient OTA-degrading activity of Pw ADH compared with that of ADH3. We conducted a structure-guided rational design of Pw ADH and obtained an upgraded variant, G88D, whose OTA-degrading activity was elevated by 1.2-fold. In addition, Pw ADH and the upgraded G88D were successfully expressed in the industrial yeast Pichia pastoris , and their catalytic activities were compared to those of their counterparts produced in E. coli , revealing the feasibility of producing Pw ADH and its variants in industrial yeast strains. These results illustrate the structural basis of a novel, efficient OTA-degrading amidohydrolase and will be beneficial for the development of high-efficiency OTA-degrading approaches.
研究领域:
食品科学
·
生物化学
·
结构生物学
✓ 已验证
DOI
PubMed
高影响因子
AICHE JOURNAL
·
2023
·Vol. 69
·期 11
·e18206
·
IF 3.7
Shuyue Wen,Tao Wang,Xiaomin Zhang,Weigao Xu,Xingbang Hu,Youting Wu
This article proposes for the first time the preparation of a series of amino acid ionic liquids (AAILs) via one-step hydrolysis of cheap lactams for the capture of CO 2 . The structures of the prepared AAILs are confirmed using NMR, FTIR, and ESI-MS, and their physical properties are also determined. It is found that these AAILs are reversible CO 2 absorbents with very high absorption capacities (0.15 to 0.18 g·g −1 at 313.2 K and 1.0 bar), better than almost all task-specific ionic liquids reported in literatures. The absorption mechanism is also elucidated to be a combination of 1:1 and 2:1 stoichiometric reaction of AAILs with CO 2 from NMR, FTIR, reaction equilibrium thermodynamical modeling and quantum calculations. The AAILs have the advantages of simple synthesis, high yield, and using available cheap raw materials. It is believed that this kind of AAILs have great potentials to be used as efficient CO 2 absorbents.
研究领域:
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2014
·
IF 4.517
Xiang-Dong Qing,Hai-Long Wu,Xi-Hua Zhang,Yong Li,Hui-Wen Gu,Ru-Qin Yu
Five-way high performance liquid chromatography–diode array detection (HPLC–DAD)–kinetic-pH data were obtained by recording the kinetic evolution of HPLC–DAD signals of samples at different pH values and a new fourth-order calibration method, alternating quinquelinear decomposition (AQQLD) based on pseudo-fully stretched matrix forms of the quinquelinear model, was developed. Simulated data were analyzed to investigate the performance of AQQLD in comparison with five-way parallel factor analysis (PARAFAC). The tested results demonstrated that AQQLD has the advantage of faster convergence rate and being insensitive to the excess component number adopted in the model. Then, they have been successfully applied to investigate quantitatively the kinetics of naptalam (NAP) hydrolysis in two practical systems. Additionally, the serious chromatographic peak shifts were accurately corrected by means of chromatographic peak alignment method based on abstract subspace difference. The good recoveries of NAP were obtained in these samples by selecting the time region of chromatogram. The elution time, spectral, kinetic time and pH profiles resolved by the chemometric techniques were in good agreement with experimental observations. It demonstrates the potential for the utilization of fourth-order data for some complex systems, opening up a new approach to fourth-order data generation and subsequent fourth-order calibration.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Rui Feng,Lili Ma,Juyi Zhao,Xiaochen Li,Sijun Dong,Yingying Wang,Yang Lyu,Ning Wang,Boxiang Kou,Yujia Wang,Yao Mu,Ying Pan,Dan Ma
Polyacrylamide (PAM) is a high molecular weight polymer with extensive applications. However, inefficient natural degradation of PAM results in its environmental accumulation. Here, using multi-omics analysis, we constructed the PAM biodegradation pathway in Klebsiella sp. PCX, an efficient PAM-degrading bacterium. Subsequently, two unclassified amidohydrolases (PCX00451 and PCX04581) were identified as key factors for rapid PAM biodegradation, both of which possessed much higher hydrolysis efficiency for PAM than for small molecule amide compounds. Besides, crystal structures of PCX00451 and PCX04581 were solved. Both two amidohydrolases were consisted with a twisted triosephosphateisomerase (TIM)-barrel and a smaller β-sandwich domain. And their binding pockets were in the conserved metal center of TIM-barrel domain. Moreover, Asp267 of PCX00451 and Asp282 of PCX04581 were examined as active sites for acid/base catalysis. Our research characterized the molecular mechanisms of two efficient amidohydrolases, providing theoretical basis and valuable tools for PAM bioremediation.
研究领域:
环境科学
·
微生物学
·
生物化学
·
结构生物学
✓ 已验证
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2018
·
IF 3.974
Xiaoxin Chen,Sheng He,Xiaolu Liu,Jiye Hu
Using sequential soil and liquid culture enrichments with cyprodinil as the sole carbon source, a Gram-negative cyprodinil-degrader from cyprodinil-polluted agricultural soil was isolated. The sequencing analysis of 16 S rRNA indicated that the strain showed 99% homology to Acinetobacter sp. The strain could effectively degrade cyprodinil at the neutral condition. At the initial concentrations of 10, 20, 50, 100, 150 and 200 mg L −1 in minimal medium, cyprodinil was degraded by 10, 20, 49.3, 64.2, 57 and 24 mg L −1 within 14 days, respectively. Two metabolites (4-cyclopropyl-6-methyl-2-pyrimidpyridine amine and monohydroxylated para-substitution) were identified using high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS/MS). A biodegradation pathway involving imines hydrolysis and monohydroxyl substitution on benzene ring was proposed on basis of the identified metabolites. Acinetobacter sp. would have a potential application in bioremediation of cyprodinil-contaminated soil, and the strain might have important implications in detoxification and bioremediation of pyrimidine analogues.
研究领域:
环境微生物学
·
生物修复
·
农药降解
·
土壤科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Horticulture Research
·
2022
·Vol. 10
·期 1
·
IF 7.291
Hu Zhangjian,Shi Junying,Feng Shuxian,Wu Xiaodan,Shao Shujun,Shi Kai
The ubiquitous lipid-derived molecules N-acylethanolamines (NAEs) have multiple immune functions in mammals, but their roles and mechanisms in plant defense response during changing environment remain largely unclear. Here, we found that exogenous NAE18:0 and NAE18:2 promoted defense against the necrotrophic pathogen Botrytis cinerea but suppressed defense to the hemi-biotrophic pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 in tomato. The knocking-down and overexpression function analysis of the pathogen-responsive NAE synthetic gene PHOSPHOLIPASE Dγ (PLDγ) and hydrolytic gene FATTY ACID AMID HYDROLASE 1 (FAAH1) revealed that the NAE pathway is crucial for plant defense response. Using exogenous applications and SA-abolished NahG plants, we unveiled the antagonistic relationship between NAE and SA in plant defense response. Elevated CO2 and temperature significantly changed the NAE pathway in response to pathogens, while inhibition of the NAE pathway led to the alternation of environment-mediated defense variations against Pst DC3000 in tomato, indicating that NAE pathway is associated with plant defense variations in response to elevated CO2 and temperature. The results herein reveal a new function of NAE in plant defense, and its involvement in environment-mediated defense variation in tomato. These findings shed light on the NAE-based plant defense, which may have relevance to crop disease management in future changing climate.
研究领域:
植物科学
·
分子生物学
·
环境科学
·
农学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2025
·
IF 1.9
Jiaping Ge,Xiaojun Huang,Zhengyu Zhang
L-asparaginase (ASNase), as a pivotal amidohydrolase enzyme, has been used in removing acrylamide in food processing and treating acute lymphoblastic leukemia in clinic. In this study, a novel ASNase from Bifidobacterium thermophilum (BtASNase) was successfully cloned and heterologously expressed in Escherichia coli. BtASNase was identified to share maximum 40% structural similarity with other ASNases in PDB database. The purified BtASNase with monomeric size about 35 kDa had the highest specific activity (554.82 IU/mg) at 55 °C and pH 8.0. Further investigation indicated that BtASNase showed great stability at wide pH range (6.0–11.0), and retained more than 85% of its activity for 50 min at 37 °C. To be noted, BtASNase exhibited high L-asparaginase specificity and zero glutaminase activity. To our knowledge, this is a novel exploration of ASNase from Bifidobacterium thermophilum, and the explored BtASNase could be a potential candidate with desirable advantages for overcoming limitations such as glutaminase activity, narrow pH range stability, and low thermostability restrict in industry applications of ASNase.
研究领域:
酶学
·
生物技术
·
分子生物学
·
食品科学
·
临床肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2019
·
IF 5.383
Manigandan Ramadoss,Yuanfu Chen,Yang Hu,Dongxu Yang
It is still challenging to develop highly efficient catalysts for oxygen evolution reaction due to their sluggish kinetics for water splitting. To address such issue, herein, for the first time, self-assembled three-dimensional porous nanoarchitecture, constructed by ultrathin cobalt-nickel-boron oxide (NiCoBO x ) nanosheets , is synthesized by a facile borohydride/amine hydrolysis strategy. As an oxygen evolution electrocatalyst , the NiCoBO x nanoarchitecture delivers excellent performance with a very low overpotential of 290 mV to achieve 10 mA cm −2 and a small Tafel slope of 59.2 mV dec −1 in an alkaline medium, which is analogous to the performance of RuO 2 . Moreover, it shows outstanding physiochemical long-term stability with excellent current density retention even after 70 h. Its OER performance is reassessed to the benchmark catalyst and practically very virtuous within most reported Ni/Co-based OER electrocatalysts. The rationally designed 3D porous nanoarchitecture of borate anchored ultrathin NiCoO x nanosheets (NiCoB)-O x favours the transportation of electrons and diffusion of ions during catalysis process, thus significantly enhancing its catalytic activity. This work provides a facile and low-cost synthesis strategy to fabricate 3D porous electrocatalyst with rational nanoarchitecture for highly efficient and stable OER.
研究领域:
电化学
·
材料科学
·
纳米技术
·
催化
✓ 已验证
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Zheng-Wei Zhang,Pei Han,Jie Fu,Hang Yu,Hui Xu,Jia-Chun Hu,Jin-Yue Lu,Xin-Yu Yang,Hao-Jian Zhang,Meng-Meng Bu,Jian-Dong Jiang,Yan Wang
Ethnopharmacological relevance Traditional Chinese medicines (TCMs) are often prepared in oral dosage forms, making TCMs interact with gut microbiota after oral administration, which could affect the therapeutic effect of TCM. Xiaoyao Pills (XYPs) are a commonly used TCM in China to treat depression. The biological underpinnings, however, are still in its infancy due to its complex chemical composition. Aim of the study The study aims to explore XYPs’ underlying antidepressant mechanism from both in vivo and in vitro. Materials and methods XYPs were composed of 8 herbs, including the root of Bupleurum chinense DC. , the root of Angelica sinensis (Oliv.) Diels, the root of Paeonia lactiflora Pall., the sclerotia of Poria cocos (Schw.) Wolf, the rhizome of Glycyrrhiza uralensis Fisch., the leaves of Mentha haplocalyx Briq., the rhizome of Atractylis lancea var. chinensis (Bunge) Kitam., and the rhizome of Zingiber officinale Roscoe, in a ratio of 5:5:5:5:4:1:5:5. The chronic unpredictable mild stress (CUMS) rat models were established. After that, the sucrose preference test (SPT) was carried out to evaluate if the rats were depressed. After 28 days of treatment, the forced swimming test and SPT were carried out to evaluate the antidepressant efficacy of XYPs. The feces, brain and plasma were taken out for 16SrRNA gene sequencing analysis, untargeted metabolomics and gut microbiota transformation analysis. Results The results revealed multiple pathways affected by XYPs. Among them, the hydrolysis of fatty acids amide in brain decreased most significant via XYPs treatment. Moreover, the XYPs' metabolites which mainly derived from gut microbiota (benzoic acid, liquiritigenin , glycyrrhetinic acid and saikogenin D) were found in plasma and brain of CUMS rats and could inhibit the levels of FAAH in brain, which contributed to XYPs’ antidepressant effect. Conclusions The potential antidepressant mechanism of XYPs by untargeted metabolomics combined with gut microbiota-transformation analysis was revealed, which further support the theory of gut-brain axis and provide valuable evidence of the drug discovery .
研究领域:
药理学
·
神经科学
·
微生物学
·
中医药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2021
·
IF 4.126
Manigandan Ramadoss,Yuanfu Chen,Xin Chen,Zhe Su,Marimuthu Karpuraranjith,Dongxu Yang,Manzoor Ahmad Pandit,Krishnamurthi Muralidharan
Rational construction of self-template catalysts for efficient electrolytic water splitting reactions is being a challenging prospect in sustainable energy production. For the first time, owing to these aims, we report a self-template representation of self-assembled iron-modulated cobalt-nickel phosphide (Fe-CoNiP) grown on 3D-nickel foam (NF) using a facile amine hydrolysis-approached synergetic phosphorylation strategy. Due to its unique vertically standing self-template hierarchical nanoarrays and atomic modulated multicomponent system, these Fe-CoNiP nanoarchitectures exhibit excellent electrocatalytic hydrogen and oxygen evolution reactions with very low applied overpotentials of 110 and 280 mV to obtain 10 mA cm–2, respectively, and an overpotential value of 390 mV (J10) obtained for overall water splitting overtakes most precious-metal/phosphide-based electrocatalysts in an alkaline medium. Moreover, the rationally cocrystallized Fe-CoNiP binder-free electrocatalyst as a multifunctional electrode has robust physicochemical stability above 2 days with very little degradation during alkaline electrolysis. These demonstrated results may lead to new insights into constructing an alternative electrocatalyst with hierarchical nanoarchitecture to boost the overall water splitting.
研究领域:
材料科学
·
电化学
·
纳米技术
·
能源材料
·
催化
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Jiaqi Xie,Yihong Li,Yulu Liang,Hongqian Kui,Can Wang,Jianmei Huang
Ethnopharmacological relevance Platycladi Semen was recorded in Shen Nong's Herbal Classic and was considered a herbal medicine with low toxicity after long-term medication. Multiple traditional Chinese medicine prescriptions containing Platycladi Semen have been used to treat insomnia. Modern clinical practitioners commonly use Platycladi Semen to treat anxiety disorders, but there are few studies on its composition and anxiolytic mechanisms. Aim of the study To describe the main components of Platycladi Semen and investigate its anxiolytic effects and mechanisms. Materials and methods The main components of Platycladi Semen were characterized by liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS). The anxiolytic effects of oral Platycladi Semen were evaluated in chronic unpredictable mild stress (CUMS) induced mice. To explore the anxiolytic mechanisms of Platycladi Semen , serum non-targeted metabolomics combined with network pharmacology and molecular docking was performed. Results Fourteen compounds were identified in the 50% methanol extract and 11 fatty acid derivatives were identified in the methyl-esterified fatty oil of Platycladi Semen . In CUMS mice, both the aqueous extract and fatty oil of Platycladi Semen had anxiolytic effects, which were shown by the increase in the time and frequency of mice entering the open arm in the elevated plus maze (EPM) experiment. Through serum non-targeted metabolomics, 34 differential metabolites were identified, and lipid metabolic pathways such as sphingolipid metabolism, steroidogenesis, alpha-linoleic acid, and linoleic acid metabolism were enriched. Through network pharmacology, 109 targets of the main components in Platycladi Semen were identified, and the ‘neuroactive ligand-receptor interaction’ and ‘lipid metabolism’ were enriched. The molecular docking results showed that the main components in Platycladi Semen could bind to the key targets such as peroxisome proliferator-activated receptor delta (PPARD), peroxisome proliferator-activated receptor alpha (PPARA), fatty acid binding protein 5 (FABP5), fatty acid binding protein 3 (FABP3), peroxisome proliferator-activated receptor gamma (PPARG), arachidonate 5-lipoxygenase (ALOX5) and fatty acid amide hydrolase (FAAH). Conclusion This study indicated that Platycladi Semen has anxiolytic effects, and the anxiolytic mechanisms may be the regulation of lipid metabolism and the neuroactive ligand-receptor interaction.
研究领域:
药理学
·
代谢组学
·
中药学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）