Microorganisms
·
2025
·Vol. 13
·期 7
·1698
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IF 4.2
Yue Cheng,Qian Fu,Junjia Xu,Xinhua Niu,Lin Liu,Jiaqi Wang,Jingwen Quan,Qingyue Yu,Baoyan Chi,Haitao Li,Rongmei Liu
Prometryn and acetochlor are herbicides used to control weeds in farmlands and other areas. They enter the soil through direct application, residual accumulation in crops, and atmospheric deposition. The pollution of their residues in the environment has attracted people’s attention. Bioremediation is one of the main methods to solve such problems. In this study, the effects of prometryn and acetochlor-degrading strain NM-1 on soil enzymes, soil microbial communities, and physiological indexes of soybean seedlings during soil remediation were studied, and the relationship between them was discussed. The results showed that 81.54% of prometryn (50 mg·L−1) and 89.47% of acetochlor (50 mg·L−1) were degraded within 15 days after NM-1 inoculation in soil. NM-1 positively affected soil enzyme activities and soil microbial communities, and the abundance of beneficial bacteria in soil increased. More importantly, the inoculation of strain NM-1 under prometryn and acetochlor stress significantly increased plant height, root length, root volume, water content, chlorophyll concentration, and root activity of soybean. The results of these studies showed that the NM-1 strain showed significant potential in bioremediation in order to provide technical support for solving the problem of prometryn and acetochlor pollution.
研究领域:
环境微生物学
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土壤科学
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生物修复
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农业化学
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2019
·
IF 2.584
Ruijie Yang,Kelin Song,Jiansheng He,Yingying Fan,Rongshu Zhu
In this work, the study of photocatalytic hydrogen production by RGO/ZnIn2S4 with simultaneous degradation of organic amines was carried out in the presence of organic amines in wastewater as the sacrificial agents. The effects of several factors, such as organic amine types, pH value, catalyst concentration, organic amine concentration, and sunlight source, on the photocatalytic activity of RGO/ZnIn2S4 for H2 production were investigated. At the same time, its performance of degrading organic amines during H2 production was also examined. The results showed that the order of H2 production activity of RGO/ZnIn2S4 in six organic amine solutions was N(CH2CH3)3 > N(HOCH2CH2)3 > N(CH3)3 > HO(CH2)2NH2 > C6H5–N2> CO(NH2)2, and the highest H2 production was in N(CH2CH3)3 (triethylamine) solution, being 1597 μmol·g–1·h–1, which is 2.6 times as high as that using the aqueous solution mixture of Na2S and Na2SO3 as the sacrificial agent. In addition, when the pH was 13, the catalyst concentration was 1.0 g·L–1, and the triethylamine concentration was 1.0 mol·L–1, the photocatalytic activity was the highest. Furthermore, the relationship between triethylamine concentration and H2 production was analyzed according to the theory of dynamics.
研究领域:
环境科学
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材料科学
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化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Wubin Liao,Peng Liao,Zheng Wang,Chenggang Ci,Shiwei Xie
The dephosphorylation of N-containing phosphonates is crucial for mitigating their potential ecological risks and enabling phosphorus resource recovery. Here, we first show that the electrochlorination process outperforms other advanced oxidation methods in terms of conversion efficiency and rate constants for orthophosphate production. The high efficacy of electrochlorination process is primarily attributed to the generation of hypochlorous acid (HClO) under neutral pH conditions, as substantiated by a combination of speciation analysis, quantitative assessments, and quenching experiments. Furthermore, density functional theory (DFT) calculation and LC-MS measurements show that the degradation of nitrilotris-methylenephosphonic acid (NTMP) by HClO occurs spontaneously, with a preferential cleavage of the C P bond over the C N bond, thereby facilitating the efficiency of the dephosphorylation reaction. Altogether, our findings provide key insights into the role of reactive chlorine species in the degradation of N-containing phosphonates, offering valuable evidence for the development of next-generation, more effective phosphorus recovery strategies.
研究领域:
环境科学
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化学工程
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计算化学
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产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2018
·
IF 3.974
Fei Li,Lin Di,Yuxin Liu,Qiuyun Xiao,Xiaoyu Zhang,Fuying Ma,Hongbo Yu
Although ascomycetes occupy a vaster niche in soil than the well-studied basidiomycetes , they have received limited attention in studies related to bioremediation. In this study, the degradation of carbaryl by Xylaria sp. was studied in different culture conditions and its possible metabolic pathway was elucidated. In liquid culture, 99% of the added carbaryl was eliminated when cytochrome P450 (CYP450) was active, which was similar to the degradation rate of Pleurotus ostreatus , a fungus with strong bioremediation ability. Mn 2+ is beneficial to the degradation of carbaryl. Compared to the 72.17% degradation rate in sterile soil, 59.0% carbaryl was eliminated in non-sterile soil, which suggested that Xylaria sp. BNL1 can resist microorganismal infection. Furthermore, the intracellular fractions containing laccase, CYP450, and carbaryl esterase efficiently degraded carbaryl. The presence of carbaryl metabolites suggested that Xylaria sp. BNL1 initiated its attack on carbaryl via carbaryl esterase to release α-naphthol, which was further degraded to 1,4-naphthoquinone and benzoic acid by CYP450 and laccase. Thus, our study highlights the potential of using Xylaria sp. for bioremediation.
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2013
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IF 3.473
Guangyu Zhao,Xiwu Lu,Yi Zhou
The use of combination of air and UV irradiation for organic matters removal has drawn attention since degradation efficiency would be significantly enhanced in the presence of oxygen. Considering the fact that a low-pressure mercury UV lamp (emitting at 254 nm and 185 nm) could dissociate oxygen in gas phase and generate ozone, UV irradiation with addition of ozone enriched air, generated by the same UV lamp, was introduced. This technology was called UV-microO 3 because the concentration of ozone generated by UV irradiation was relatively low (lower than 1 mg L −1 ). The degradation of aniline by UV-aeration and UV-microO 3 processes was comparatively analyzed. It was shown that aniline underwent much faster and more complete degradation in UV-microO 3 process than in UV-aeration system. Hydroxyl radical ( OH) exposure in UV-microO 3 process was 2.46 times of that in UV-aeration system. Furthermore, increasing inlet air flow rate would be of benefit to maintaining high dissolved oxygen (DO) level in UV-aeration system, thus the aniline removal was improved. While concentration of ozone generated by UV irradiation increased at first and then declined as the air flow rate increased from 1.8 L min −1 to 4.8 L min −1 in UV-microO 3 process, and similar trend for aniline removal rate was observed. The contributions of radicals on aniline removal in UV-aeration and UV-microO 3 processes were calculated to be 23.1% and 67.7%. The formation of 4-aminophenol, 4-Hydroxydiphenylamine, azobenzene and 4-Phenylazophenol was observed as the major intermediates in UV-aeration and UV-microO 3 processes, mechanism of aniline degradation was proposed based on experimental evidence.
研究领域:
环境科学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Tianyuan Xu,Ting Chen,Zhenhua Chen,Yijun Cao,Yaowen Xing,Xiahui Gui
A photochemical system combining iron (hydr)oxides and oxalate (Ox) shows application prospects in wastewater treatment due to the abundance of reactive oxygen species (ROS) generation. Nevertheless, it is a challenge to the investigate photochemical activity of iron (hydr)oxides/Ox systems with varying structural properties. Herein, the photochemical behaviors of Ox on goethite (Gt) surface from the view of structural dependent activity, containment degradation, and ROS generation were explored in detail. Results confirmed that bidentate mononuclear was formed on Gt surface after complexing Ox. Combined with density functional theory calculation and pH time evolution during aniline degradation, the photochemical activity of the Gt/Ox system fell in between that of ferrihydrite/Ox and hematite/Ox systems. After irradiating 120 min visible light, 96.5% aniline was degraded by 1.0 mM Ox and 0.2 g/L Gt. The amount of •OH in vis/Gt/Ox system could be up to 309.3 μM and its generation was closely associated with Fe(II) while slightly affected by the generated H 2 O 2 . Moreover, as revealed by high-performance liquid chromatography with mass spectrometric and Ecological Structure Activity Relationships software, the toxicity of the intermediates of aniline degradation in the vis/Gt/Ox system towards fish and green algae increased first but then declined accompanied by the generation of non-toxic ring-opening products at the end of reaction. According to the findings in the presented study, it could be concluded that vis/Gt/Ox is a promising approach to wiping out aniline wastewater.
研究领域:
环境科学
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化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
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2021
·
IF 6.498
Li Li,Ruoning Guo,Sai Zhang,Yiming Yuan
In recent decades, sodium percarbonate (SPC) has been widely applied as a solid replacement for H 2 O 2 in advanced oxidation process (AOPs). In this study, ultraviolet (UV) light was utilized for SPC activation to investigate the aniline degradation performance. The effects of SPC dosages and UV irradiation on aniline degradation were elaborated. The removal efficiency was significantly improved by increasing either the SPC dosage or UV irradiation intensity. Moreover, scavenging experiments showed that •OH, CO 3 •− , and O 2 •− were the predominant reactive species for aniline degradation. Meanwhile, the variation in the amount of •OH in the UV/SPC system was monitored, which revealed the dominant role of •OH. As a result, the mechanism of aniline degradation by the UV/SPC system was demonstrated based on confirmed free radicals. Furthermore, aniline degradation by the UV/H 2 O 2 and UV/H 2 O 2 /Na 2 CO 3 system were compared with the UV/SPC system, and an enhancement by the addition of Fe(II) in the UV/SPC system was verified. Aniline degradation was not significantly affected by the initial pH or the presence of Cl − , SO 4 2− while NO 3 − , HCO 3 − and humid acid (HA) suppressed the reaction. In general, the UV/SPC system is a novel, green, and promising technology for aniline removal from aqueous solutions.
研究领域:
环境科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2019
·
IF 5.108
Bin Ou,Jixiao Wang,Ying Wu,Song Zhao,Zhi Wang
The graphite felt (GF) was modified through the concentrated sulfuric acid , potassium permanganate and ammonia activation with the aim of improving the specific surface area, hydrophilicity and the electrocatalytic activity for the electrogeneration of hydrogen peroxide and its application in the electro-Fenton (EF). The modified graphite felt (M-GF) was characterized by contact angle, scanning electron microscope , X-ray photoelectron spectroscopy, Raman spectrum , cyclic voltammetry and electrochemical impedance spectroscopy. The surface physic-chemical characteristics and morphology of the M-GF were considerably changed after the modification. The current efficiency for H 2 O 2 generation was explored at an optimized current density of 10 mA cm −2 and the value of H 2 O 2 generation for the M-GF was 478.6 mg L −1 , while the GF was 276.5 mg L −1 after 360 min of electrolysis. Finally, the M-GF was used for the mineralization of aniline (100 mg L −1 ), reaching a mineralization percentage of 97% with EF process. The M-GF demonstrated good stability as the aniline mineralization percentage was above 90% after 10 cycles’ degradation, indicating that the M-GF is a promising cathode material for improving the degradation and mineralization of others organic pollutants by the EF process.
研究领域:
环境科学
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材料科学
✓ 已验证
DOI
高影响因子
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2019
·Vol. 85
·期 12
·
IF 4.077
Xinyu Lu,Weiwei Wang,Lige Zhang,Haiyang Hu,Ping Xu,Tao Wei,Hongzhi Tang
N,N-Dimethylformamide (DMF) is one of the most common xenobiotic chemicals, and it can be easily emitted into the environment, where it causes harm to human beings. Herein, an efficient DMF-degrading strain, DM1, was isolated and identified as Methylobacterium sp. This strain can use DMF as the sole source of carbon and nitrogen. Whole-genome sequencing of strain DM1 revealed that it has a 5.66-Mbp chromosome and a 200-kbp megaplasmid. The plasmid pLVM1 specifically harbors the genes essential for the initial steps of DMF degradation, and the chromosome carries the genes facilitating subsequent methylotrophic metabolism. Through analysis of the transcriptome sequencing data, the complete mineralization pathway and redundant gene clusters of DMF degradation were elucidated. The dimethylformamidase (DMFase) gene was heterologously expressed, and DMFase was purified and characterized. Plasmid pLVM1 is catabolically crucial for DMF utilization, as evidenced by the phenotype identification of the plasmid-free strain. This study systematically elucidates the molecular mechanisms of DMF degradation by Methylobacterium.IMPORTANCE DMF is a hazardous pollutant that has been used in the chemical industry, pharmaceutical manufacturing, and agriculture. Biodegradation as a method for removing DMF has received increasing attention. Here, we identified an efficient DMF degrader, Methylobacterium sp. strain DM1, and characterized the complete DMF mineralization pathway and enzymatic properties of DMFase in this strain. This study provides insights into the molecular mechanisms and evolutionary advantage of DMF degradation facilitated by plasmid pLVM1 and redundant genes in strain DM1, suggesting the emergence of new ecotypes of Methylobacterium.
研究领域:
环境微生物学
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生物化学
✓ 已验证
DOI
高影响因子
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2022
·Vol. 88
·期 4
·
IF 5.005
Zhijian Ke,Qian Zhu,Siyuan Gao,Mingliang Zhang,Mingli Jiang,Yijun Ren,Yali Liu,Yidong Zhou,Jiguo Qiu,Qing Hong
Previously, a LysR family transcriptional regulator, McbG, that activates the mcbBCDEF gene cluster involved in the upstream pathway (from carbaryl to salicylate) of carbaryl degradation in Pseudomonas sp. strain XWY-1 was identified by us (Z. Ke, Y. Zhou, W. Jiang, M. Zhang, et al., Appl Environ Microbiol 87:e02970-20, 2021, https://doi.org/10.1128/AEM.02970-20). In this study, we identified McbH and McbN, which activate the mcbIJKLM cluster (responsible for the midstream pathway, from salicylate to gentisate) and the mcbOPQ cluster (responsible for the downstream pathway, from gentisate to pyruvate and fumarate), respectively. They both belong to the LysR family of transcriptional regulators. Gene disruption and complementation study reveal that McbH is essential for transcription of the mcbIJKLM cluster in response to salicylate and McbN is indispensable for the transcription of the mcbOPQ cluster in response to gentisate. The results of electrophoretic mobility shift assay (EMSA) and DNase I footprinting showed that McbH binds to the 52-bp motif in the mcbIJKLM promoter area and McbN binds to the 58-bp motif in the mcbOPQ promoter area. The key sequence of McbH binding to the mcbIJKLM promoter is a 13-bp motif that conforms to the typical characteristics of the LysR family. However, the 12-bp motif that is different from the typical characteristics of the LysR family regulator binding site sequence is identified as the key sequence for McbN to bind to the mcbOPQ promoter. This study revealed the regulatory mechanisms for the midstream and downstream pathways of carbaryl degradation in strain XWY-1 and further our knowledge of (and the size of) the LysR transcription regulator family.IMPORTANCE The enzyme-encoding genes involved in the complete degradation pathway of carbaryl in Pseudomonas sp. strain XWY-1 include mcbABCDEF, mcbIJKLM, and mcbOPQ. Previous studies demonstrated that the mcbA gene, responsible for hydrolysis of carbaryl to 1-naphthol, is constitutively expressed and that the transcription of mcbBCDEF was regulated by McbG. However, the transcription regulation mechanisms of mcbIJKLM and mcbOPQ have not been investigated yet. In this study, we identified two LysR-type transcriptional regulators, McbH and McbN, which activate the mcbIJKLM cluster (responsible for the degradation of salicylate to gentisate) and the mcbOPQ cluster (responsible for the degradation of gentisate to pyruvate and fumarate), respectively. The 13-bp motif is critical for McbH to bind to the promoter of mcbIJKLM, and 12-bp motif different from the typical characteristics of the LysR-type transcriptional regulator (LTTR) binding sequence affects the binding of McbN to the promoter. These findings help to expand the understanding of the regulatory mechanism of microbial degradation of carbaryl.
研究领域:
微生物学
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分子生物学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2018
·
IF 4.427
Yao Wu,Jing Guo,Yijie Han,Junyi Zhu,Lixiang Zhou,Yeqing Lan
This study investigated the degradation of aniline by persulfate (PS) activated with rice straw biochar (RSBC). The results demonstrate that aniline could be rapidly decomposed by a combination of PS and RSBC. The degradation efficiency of aniline was up to 94.1% within 80 min, and meanwhile 52% of the total organic carbon was removed. In the initial pH range of 3–9, aniline could be efficiently removed. Reactive species resulting in the rapid degradation of aniline were investigated via radical and hole quenching experiments with various scavengers (e.g., methanol, tert -butyl alcohol and EDTA) and electron paramagnetic resonance technique. Based on the analysis and observation made here, it is speculated that the predominant reactive species responsible for the degradation of aniline may be holes instead of SO 4 − and OH radicals. It is concluded that RSBC could be used as an effective catalyst to activate PS for the degradation of aniline.
研究领域:
环境科学
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化学工程
✓ 已验证
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2018
·
IF 4.427
Li Li,Jun Huang,Xuebin Hu,Sai Zhang,Qin Dai,Hongxiang Chai,Li Gu
Sodium percarbonate (SPC)-based advanced oxidation process has been applied to the wastewater treatment in recent years. In the present study, a novel catalyst utilization of vanadium(V) was investigated for the activation of SPC for aniline degradation. The mechanism of SPC activation by V(IV) was demonstrated, and the major free radicals were identified through scavenging tests and electron paramagnetic resonance (EPR) analysis. The performance of aniline degradation was evaluated in the V(IV)/H 2 O 2 and V(IV)/H 2 O 2 /Na 2 CO 3 systems and compared with that of the V(IV)/SPC system. The influences of initial pH and effects of inorganic anions were also studied. The results show that aniline could be decomposed efficiently by SPC activated with V(IV) and the degradation efficiency increased with the increase in V(IV) and SPC dosage. O 2 − , CO 3 − , and OH were found to participate in aniline degradation, and O 2 − and CO 3 − were confirmed to be the predominant species. The decomposition of aniline was enhanced when equal amount of H 2 O 2 was utilized instead of SPC under acidic condition while a comparative behavior was achieved in the V(IV)/H 2 O 2 /Na 2 CO 3 system. Furthermore, aniline degradation was not impacted significantly by the initial pH and addition of Cl − , SO 4 2− and NO 3 − , while HCO 3 − led to a betterment of aniline removal. These results indicate that the V(IV)/SPC system is an effective and promising approach for the removal of aniline from water for its feasibility and stability, which achieves eliminating contaminants by another waste.
研究领域:
环境科学
·
化学工程
✓ 已验证
DOI
PubMed
高影响因子
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