Journal of Environmental Chemical Engineering
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2021
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IF 5.909
Deyong Che,Liye Wang,Hongpeng Liu,Baizhong Sun,Shuai Guo
Biomass pyrolysis has attracted attention; however, its development has been restricted by the formation of NOx precursors, which result mainly from protein pyrolysis. In addition, the effect of lipids on this process is not clear. We extracted proteins and lipids from chlorella and sewage sludge to investigate the thermal decomposition characteristics, thermodynamic properties, kinetic parameters, and reaction mechanisms of protein with or without lipids. Two model-free methods Flynn-Wall-Ozawa (FWO) and Friedman (FM) were used to calculate the kinetic energy. Between 150 and 600 ℃, the average activation energy (E) of sludge protein was 73.63 kJ/mol (FM) or 72.53 kJ/mol (FWO), whereas with lipids, it was 129.19 kJ/mol (FM) or 124.55 kJ/mol (FWO), indicating that lipids restrict the pyrolysis of sludge protein. There was no significant difference between E of chlorella protein pyrolysis with or without lipids. Moreover, the proteins in sludge and chlorella released less NH 3 under the influence of lipids; however, the formation of HCN was affected slightly. The results of this study provide a reliable reference for the regulation of protein pyrolysis, considering the production of NOx precursors.
研究领域:
环境科学
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生物能工程
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DOI
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Xi Cao,Yayu Pan,Miao Qiao,Yongjun Yuan
Rapeseed oil has a similar oleic acid/linoleic acid ratio to human milk fat (HMF). However, it can hardly be used for human milk fat substitute (HMFS) synthesis due to high erucic acid content. In this study, Candida cylindracea lipase (CCL) was found to strongly discriminate against erucic acid. Free fatty acids containing low erucic acid and high oleic acid and linoleic acid were prepared from rapeseed oil hydrolysis catalyzed by CCL. The erucic acid content was only 1.58% (initial 8.70%), when the degree of hydrolysis reached 79.58%. The free fatty acids were used as acyl-donors in the acidolysis catalyzed by Novozym 40086. Considering acyl incorporation and migration, the optimum conditions were 1:8 (tripalmitin to acyl-donors), 40 °C and 2 h. The erucic acid content dropped to 0.97% in the HMFS. According to the Q-TOF-MS analysis, the HMFS was rich in 1,3-dioleoyl-2-palmitoyl-glycerol (18.20%) and 1-oleoyl-2-palmitoyl-3-linoleoyl-glycerol (17.96%), which was similar to HMF.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2020
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IF 5.774
Yujing Tang,Qianqian Ma,Yulu Du,Lu Ren,Leonardo J. Van Zyl,Xuwei Long
Efficient synthesis and purification of sophorolipids produced by fermentation into ester and free acid derivatives has attracted significant interest as these compounds have many potential specific applications. In this study, the ester and free acid derivatives of sophorolipids were prepared via chemical modifications, and the purification via a series of stepwise extractions was optimized. Acidic type sophorolipids (SL-COOH) was readily obtained by hydrolysis of sophorolipids isolated from fermentation broth, while the lactonic sophorolipids (SL-ester) can be prepared by the esterification of SL-COOH. Then, a series of newly developed stepwise extractions using optimized solvent systems recovered more than 50% of the SL-COOH and about 80% of the SL-ester with high purity, respectively. Both modified SLs have great surface activities, can reduce the surface tension to 40–43 mN/m with critical micellar concentrations ranged from 50 to 120 mg/L. More importantly, SL-ester presented excellent antibacterial activity against B. subtilis and Moesziomyces sp ., and the inhibition efficiency reached over 96% and 83% at concentration of 1 g/L, was much better than that by natural SLs and SL-COOH. Such chemical modifications followed by the newly developed purification would be conducive to the practical applications of sophorolipids in several fields, especially as antibacterial agents.
研究领域:
生物化学
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微生物学
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应用化学
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DOI
高影响因子
SCIENTIA HORTICULTURAE
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2017
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IF 1.624
Xueyuan Han,Linchun Mao,Xiaopeng Wei,Wenjing Lu
Wound-induced suberization is an essentially protective healing process for fruit to reduce water loss and avoid infecting. However, cognate mechanisms that regulate this process are little known. To expand our knowledge of suberization induced by wounding, a wound-healing investigation together with metabolite profiling study was conducted in postharvest kiwifruit ( Actinidia deliciosa ). The development of suberization in wounded fruit was demonstrated by autofluorescence observation and toluidine blue staining at 1–4 day (d) after wounding. Activities of phenylalanine ammonia-lyase (PAL), cinnamyl-alcohol dehydrogenase (CAD) and peroxidase (POD) in wound-healing tissue were enhanced by abscisic acid (ABA). The constituent analysis of suberin including polyphenolics (SPP) and polyaliphatics (SPA) proved that exogenous ABA increased the content levels of total phenols, total flavonoids and alkanes, alkenes, alcohols, alkane acids, olefine acids, esters, glycerides and vitamin E in wound-healing tissue. Results suggested that ABA stimulated suberization through the activation of PAL, CAD and POD to accelerate wound-healing of wounded kiwifruit.
研究领域:
植物生理学
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园艺学
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食品科学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2021
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IF 3.205
Xuechen Liu,Jingchen Wang,Bingying Hu,Pan Yan,Shuailong Jia,Zhifeng Du,Hongliang Jiang
Sphingolipids (SLs) are endogenously bioactive molecules with diverse structures, and its metabolic disorders are involved in the progression of many diseases. In this study, an ultra-performance liquid chromatography quadrupole exactive mass spectrometry (UPLC-Q-Exactive-MS) method was established to comprehensively profile SLs in plasma. First, the fragment patterns of SL standards of each subclass were investigated. Then, the SL species in plasma were characterized based on the fragmentation rules. Finally, a total of 144 endogenous SL species consisting of 216 regioisomers were identified in plasma of human, golden hamster and C57BL/6 mice, which was the most comprehensive identification for SLs in plasma. In addition to the known species, 19 SL species that have never been reported were also identified. The profile of SLs in plasma of human and two rodent species was compared subsequently. It was worth noting that a total of 9 SL molecular species consisting of 11 regioisomers with low abundance were successfully identified in human plasma through comparison among species. Those findings contribute to a deeper understanding of SLs in human plasma and provide scientific basis for the selection of animal model. The established profile of SLs in plasma could be used for screening of lipid biomarkers of various diseases.
研究领域:
生物化学
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医学
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Xiangxin Xu,Xiaofang Liu,Shuai Wang,Yongping Zou,Jixian Zhang,Li Liang,Chaoting Wen,Youdong Li,Xiaowei Xu,Xudong He,Guoyan Liu,Xin Xu
Lipids are closely related to the generation of PAHs during food thermal processing. During heating, lipids mainly triglycerides undergo hydrolysis, oxidation and decomposition. The relationship between the various products and the formation of PAHs is still unclear. This paper investigated the effect of different lipid standards on PAH4 production, and explored their thermal stability and reaction products to delve into nature of the differences in PAH4 production. Fatty acids were more prone to generate PAH4 than glycerides. The higher the degree of esterification of glycerides, the higher its thermal stability and the lower the content of PAH4 generated, implying that hydrolysis of glycerides promoted the generation of PAH4. In addition, there was a positive correlation between unsaturation in lipids and the PAH4 production. After heat treatment, hydroperoxides, unsaturated fatty alcohols and aldehydes, alkenes and aromatic substances were abundant in oleic acid and linoleic acid which produced the most PAH4. Thermal decomposition of lipid hydroperoxides was the pathway for the generation of conjugated hydrocarbon radicals, alcohols, aldehydes, and alkenes . The intramolecular cyclization and Diels-Alder reaction acted as ring-forming reactions, with consequent dehydrogenation , decarboxylation , side-chain breaks and radical reorganization, ultimately facilitating the amplification of the aromatic rings and the formation of PAHs.
研究领域:
食品科学
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化学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
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2022
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IF 8.943
Lingfei Ma,Fangyuan Peng,Qingqing Dong,Haipu Li,Zhaoguang Yang
Disinfection byproducts (DBPs) remains an ongoing issue because of their widespread occurrence and toxicity. Various organic substances in Algogenic organic matter (AOM) can produce DBPs in the chlorination process. To provide specific suggestions for the targeted removal of DBP precursors in AOM, the main biochemical components in AOM were qualitatively and quantitatively analyzed. An accurate model for predicting the DBP formation potentials (DBPFPs) of AOM was herein developed based on the dissolved organic carbon of the five main biochemical components in AOM and the DBPFPs of their corresponding surrogates. The contributions of each biochemical component to the three DBP species were evaluated, and the key components were identified. The results showed that lipids, proteins, carbohydrates, humic acid-like substances, and fulvic acid-like substances were the main biochemical components in AOM. Thereof, proteins (71.2 ± 2.1%) and carbohydrates (53.1 ± 2.1%) were the major contributor to the carbon content in intracellular organic matter and extracellular organic matter, respectively. The contribution results of biochemical components to the formation of DBPs showed that proteins were the key contributor to DBPs, suggesting that the targeted removal of proteins before the chlorination process would effectively reduce DBPs from AOM.
研究领域:
环境科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Molecular Therapy-Nucleic Acids
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2021
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IF 8.886
Jing Miao,Xiqin Yang,Xuwei Shang,Zhe Gao,Qian Li,Yun Hong,Jiaying Wu,Tingting Meng,Hong Yuan,Fuqiang Hu
Hepatitis B (HB) is a viral infectious disease that seriously endangers human health, and since there are no radical drugs to counter this, effective and safe therapies urgently need to be developed. HB virus (HBV) mainly infects hepatocytes (HCs), while the drugs are easily phagocytosed by Kupffer cells (KCs). In this study, the glutathione concentration difference between HCs and KCs was examined and utilized in an ideal drug-release strategy. Here, galactosylated chitosan-oligosaccharide-SS-octadecylamine (Gal-CSSO) was prepared to accurately deliver 10-23 DNAzyme DrzBC (blocking HBeAg expression) or DrzBS (blocking HBsAg expression) in targeted HB therapy. In vitro Gal-CSSO systems exhibited low cytotoxicity, endosomal escape, and glutathione responsiveness. The HBeAg and HBsAg secretion of HepG2.2.15 was significantly decreased by Gal-CSSO systems, and the maximum inhibition rates were 1.82-fold and 2.38-fold greater than those of commercial Lipofectamine 2000 (Lipo2000) systems. In vivo Gal-CSSO systems exhibited HC targeting and HC microenvironmental responsiveness without noticeable hepatotoxicity or systemic toxicity. The HBeAg and HBsAg titers of the HBV-infected mice were evidently decreased by Gal-CSSO systems, and the inhibition rates were 1.52-fold and 1.22-fold greater than those of Lipo2000 systems. This study presents a kind of glycolipid-like polymer micelles that promise efficient and safe gene therapy of HB.
研究领域:
药理学
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分子生物学
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治疗学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2024
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IF 6.2
Xuecheng Li,Changyi Li,Ziyu Chen,Jiahui Wang,Jie Sun,Jun Yao,Ke Chen,Zhenghui Li,Hengpeng Ye
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous at relatively high concentrations by atmospheric deposition, and they are threatening to the environment. In this study, the toxicity of naphthalene on tall fescue and its potential responding mechanism was first studied by integrating approaches. Tall fescue seedlings were exposed to 0, 20, and 100 mg L −1 naphthalene in a hydroponic environment for 9 days, and toxic effects were observed by the studies of general physiological studies, chlorophyll fluorescence, and root morphology. Additionally, Ultra Performance Liquid Chromatography - Electrospray Ionization - High-Resolution Mass Spectrometry (UPLC-ESI-HRMS) was used to depict metabolic profiles of tall fescue under different exposure durations of naphthalene, and the intrinsic molecular mechanism of tall fescue resistance to abiotic stresses. Tall fescue shoots were more sensitive to the toxicity of naphthalene than roots. Low-level exposure to naphthalene inhibited the electron transport from the oxygen-evolving complex (OEC) to D1 protein in tall fescue shoots but induced the growth of roots. Naphthalene induced metabolic change of tall fescue roots in 12 h, and tall fescue roots maintained the level of sphingolipids after long-term exposure to naphthalene, which may play important roles in plant resistance to abiotic stresses.
研究领域:
环境科学
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植物生理学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
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IF 6.1
Jian Zhang,Zesen Zhang,Ting Jiang,Zhenyu Zhang,Wenjing Zhang,Wei Xu
Unsaturated lipids play an essential role in life activities. Identifying and quantifying their carbon-carbon double bond (C C) isomers have become a hot topic in recent years. In lipidomics, the analysis of unsaturated lipids in complex biological samples usually requires high-throughput methods, which puts forward the requirements of rapid response and simple operation for identification. In this paper, we proposed a photoepoxidation strategy, which uses benzoin to open the double bonds of unsaturated lipids to form epoxides under ultraviolet light and aerobic conditions. Photoepoxidation is controlled by light and has a fast response. After 5 min, the derivatization yield can reach 80% with no side reaction products. Besides, the method has the advantages of high quantitation accuracy and a high yield of diagnostic ions. It was successfully applied to rapidly identify the double bond locations of various unsaturated lipids in both positive and negative ion modes, and to rapidly identify and quantitatively analyze the various isomers of unsaturated lipids in mouse tissue extract. So the method has the potential for large-scale analysis of unsaturated lipids in complex biological samples.
研究领域:
生物化学
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脂质组学
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产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
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2023
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IF 8
Yupeng Liu,Suhuan Dai,Lijuan Wen,Yun Zhu,Yanan Tan,Guoxi Qiu,Tingting Meng,Fangying Yu,Hong Yuan,Fuqiang Hu
Background Phototherapy is a potential new candidate for glioblastoma (GBM) treatment. However inadequate phototherapy due to stability of the photosensitizer and low target specificity induces the proliferation of neovascular endothelial cells for angiogenesis and causes poor prognosis.Methods In this study, we constructed c(RGDfk)-modified glycolipid-like micelles (cRGD-CSOSA) encapsulating indocyanine green (ICG) for dual-targeting neovascular endothelial cells and tumor cells, and cRGD-CSOSA/ICG mediated dual effect of PDT/PTT with NIR irradiation.Results In vitro, cRGD-CSOSA/ICG inhibited cell proliferation and blocked angiogenesis with NIR irradiation. In vivo, cRGD-CSOSA/ICG exhibited increased accumulation in neovascular endothelial cells and tumor cells. Compared with that of CSOSA, the accumulation of cRGD-CSOSA in tumor tissue was further improved after dual-targeted phototherapy pretreatment. With NIR irradiation, the tumor-inhibition rate of cRGD-CSOSA/ICG was 80.00%, significantly higher than that of ICG (9.08%) and CSOSA/ICG (42.42%). Histological evaluation showed that the tumor vessels were reduced and that the apoptosis of tumor cells increased in the cRGD-CSOSA/ICG group with NIR irradiation.Conclusion The cRGD-CSOSA/ICG nanoparticle-mediated dual-targeting phototherapy could enhance drug delivery to neovascular endothelial cells and tumor cells for anti-angiogenesis and improve the phototherapy effect of glioblastoma, providing a new strategy for glioblastoma treatment.
研究领域:
肿瘤学
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药剂学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
CATALYSIS LETTERS
·
2023
·Vol. 154
·期 5
·2386-2395
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IF 2.8
Xu Hui,Xing Feifei,Guo Yue,Wang Fei,Feng Junfeng,Li Hui,Jiang Xiaoxiang
To explore a green, cheap, and high-performance catalyst for lipid hydrotreatment into green diesel, the non-noble metal catalysts (Fe, Cu, Co, Ni, and Mo) with Al 2 O 3 carrier were prepared. Their catalytic performance in oleic acid hydrotreatment was compared, and the metal loading was studied. The characterizations (XRD, N 2 physical sorption, H 2 -TPR, and XPS) were utilized to elucidate the catalytic property. In oleic acid hydrotreatment, the catalytic activity of 5 monometallic catalysts was in the following order: Co > Mo > Ni > Fe > Cu, and the Co/Al 2 O 3 catalyst achieved 94.4% conversion and 91.8% alkane yield. Among 6 Co loadings (5, 10, 15, 20, 25, and 30 wt%), 25 wt% was optimal because it reached the balance of the surface area and the number of Co active sites. Moreover, the lipid hydrotreatment over Co/Al 2 O 3 catalyst occurred mainly via DCX/DCN route; meanwhile, the cracking reaction also happened. Graphical Abstract
研究领域:
能源化学
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催化科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）