JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 1
·e70015
·
IF 3.4
Jingxuan Yang,Yi Wu,Ziye Jiang,Lili Jiao,Yang Wang
This study aimed to investigate the potential hypoglycemic mechanism of red ginseng acidic polysaccharides (RGAP) from the perspective of fatty acid (FA) regulation. A high-glucose/high-fat diet in conjunction with streptozotocin administration was employed to establish type 2 diabetes mellitus (T2DM) rat models, and their fecal FAs were detected using the liquid chromatography-mass spectrometry (LC-MS) method. RGAP treatment alleviated the polyphagia, polydipsia, weight loss, and hyperglycemia observed in T2DM rats. FA profile was disturbed by T2DM modeling, and 11 marker FAs were selected from statistical analysis, whose intensities were reversely changed by RGAP administration. Among these marker FAs, short-chain FAs were negatively correlated with the fasting blood glucose (FBG) level, while positive correlations were observed between long-chain FA and the FBG level. Combined with the metabolite-enzyme-gene network analysis, we inferred that the mechanistic mechanism RGAP on T2DM may be associated with the regulation of FA metabolism and inflammation-related signaling pathways. This study confirmed the regulatory effect of RGAP on fecal FA, which can provide a scientific basis and new ideas for developing red ginseng as a functional food for supplementary treatment of T2DM.
研究领域:
营养学
·
代谢组学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FLUID PHASE EQUILIBRIA
·
2018
·
IF 2.197
Gang Wang,Jing Fan,Xiaopo Wang,Fenhong Song,Qing Wang,Lihui Zhang
The utilization of biodiesel, which is considered as a renewable, biodegradable and clean-burning transportation fuel consisting of fatty acid alkyl esters, is a promising way to replace fossil fuels. Thermal conductivity is a very important thermophysical property of the biodiesel. In this work, the liquid thermal conductivity of five saturated fatty acid ethyl esters (ethyl myristate, ethyl laurate, ethyl caprate, ethyl caprylate and ethyl heptanoate) were measured with the temperature ranging within (290.64–393.88) K, (274.73–400.08) K, (270.00–400.44) K, (254.76–400.37) K, (250.38–393.46) K, respectively. The total relative standard uncertainty of the experimental results was estimated to be less than 2% and repeatability was better than ±0.5%. The experimental results of each substance were fitted as a function of temperature. The average absolute relative deviation and maximum absolute relative deviation between the experimental data and calculated results were 0.19% and 0.36% for ethyl myristate, 0.15% and 0.54% for ethyl laurate, 0.17% and 0.38% for ethyl caprate, 0.23% and 0.66% for ethyl caprylate, 0.19% and 0.52% for ethyl heptanoate, respectively.
研究领域:
化学工程
·
能源科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yida Xu,Yongli Ye,Chang Liu,Biyan Chen,Jian Ji,Jiadi Sun,Yinzhi Zhang,Xiulan Sun
Steam-processed Polygonatum sibiricum (PS) has been used as food for thousands of years. However, fewer studies concentrated on the effects of polysaccharides (SPSP) from the steamed PS on the intestinal tract. With fermentation in vitro , we investigated the impact of SPSP on the fatty acids and microbiotas. Results showed significant increases in short-chain fatty acids (SCFAs) like acetic acid and propionic acid, and long-chain fatty acids (LCFAs) like cis , cis , cis -9,12,15-linolenic acid, cis -6-octadecenoic acid, and cis -9-octadecenoic acid after 12 h. The positive-associated beneficial microbiotas were observed with proliferation like Parabacteroides and Bifidobacterium . Harmful microbiota like Shigella showed decreased abundance. Further, a small molecule polysaccharide was separated from the SPSP with the structure of one glucose and ten fructose, which significantly increased SCFAs and LCFAs contents during fermentation. The potential benefits of SPSP were proved by the analysis of fatty acid levels and the intestinal microbiotas during fermentation.
研究领域:
食品科学
·
微生物学
·
植物化学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Lerong Qi,Xiangzi Li,Yachen Ren,Tingxiang Yang,Yang Guan,Dong Hao Wang,Zhen Wang
Sebum fatty acids and squalene are major components of skin secretions and play a critical role in the pathogenesis of dermatological conditions through their compositional alterations. Human sebum fatty acids are uniquely diverse in their chain length, double bond positions, and chain branching; squalene is a rare component of skin secretions with six double bonds. We introduce a facile workflow for the analysis of sebum fatty acids and squalene for elucidating their contribution to the state of skin health or disease using advanced mass spectrometric methods for qualitative and quantitative analyses. Sebum from dermatologically healthy individuals was extracted, and covalent adduct chemical ionization (CACI), electron ionization (EI), and Paternò–Büchi (PB) tandem mass spectrometry (MS/MS) techniques were employed for the identification of fatty acids with varying methyl branch and/or carbon–carbon double bond positions. A method for the full structural characterization of squalene via CACI tandem mass spectrometry was also developed. In sebum, we report for the first time unusual straight-chain polyunsaturated fatty acid isomers (n18:2n-7, n18:2n-3, n20:2n-12, and n20:2n-7), a series of previously unreported branched-chain saturated fatty acids with C19–C26, and a novel branched-chain monounsaturated fatty acid i15:1n-9. A total of 64 fatty acids were identified, exceeding the previous reports. Our study established a comprehensive qualitative and quantitative workflow for the analysis of sebum fatty acids and squalene, with the potential to advance dermatological research and clinical diagnostics.
研究领域:
皮肤病学
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脂质组学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Oleo Science
·
2017
·
IF 1.076
Peng Hu,Xuebing Xu,Liangli (Lucy) Yu
In order to obtain margarine free of trans-fatty acids, four interesterified basestocks were prepared by chemical interesterification (CIE) of oil blends. Different ratios of palm stearin, palm olein and soybean oil were mixed without and with 1) fully hydrogenated Acer truncatum oil (FHATO), 2) fully hydrogenated rapeseed oil or 3) palm kernel oil containing a similar amount of saturated, monounsaturated and polyunsaturated fatty acids, but different saturated fatty acid length for CIE. Compared to the physical blends, the CIE samples demonstrated lower slip melting points and decreased solid fat contents, especially at high temperatures, indicating that the CIE samples might have improved mouthfeel. In all CIE samples, the β crystal form disappeared and only the β’ crystal form was observed, except for sample 2, which contained a mixed β and β’ forms. Furthermore, in all CIE samples, except sample 1, the β' crystal forms began transforming to β form after only two cycles of higher temperature treatments indicating that the CIE sample with FHATO had the most resistance to temperature fluctuation during storage which may be attributed to its longer saturated chains. In conclusion, the CIE basestocks containing longer saturated fatty acids could be more suitable for margarine use.
研究领域:
食品科学
·
脂质化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Xiuming Jiang,Denghui Yang,Guoqiang Xiang,Leqian Hu
In this work, cis and trans fatty acid (FA) contents in edible oils was determined using 1 H nuclear magnetic resonance ( 1 H NMR) spectroscopy. The comparison of 1 H NMR spectra of edible oil and cis-trans isomerized oil showed that they were different in three regions: (1) 2.880 - 2.650 ppm caused by bis-allylic protons; (2) 2.140 - 1.900 ppm due to allylic protons; and (3) 1.030 - 0.930 ppm due to methyl protons of linolenic acyl groups. The 1 H NMR signals in these regions were combined with partial least squares method to predict contents of cis/trans fatty acids in edible oils. The results showed that the predicted values were consistent with the gas chromatography-measured values and had a good linear relationship with a correlation coefficient (R 2 ) of above 0.98. This work demonstrates that 1 H NMR spectroscopy was successfully used to determine the composition of both cis and trans fatty acids in edible oils.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2020
·Vol. 56
·期 5
·2492-2503
·
IF 2.773
Tian Li,Qin Guo,Yang Qu,Yujie Li,Xinping Wang,Zhigao Sun,Qiang Wang
Summary An improved gas chromatography-based analytical method for the simultaneous separation of seventy-two fatty acids was developed. Twenty-six types of trans fatty acids and conjugated linoleic acids were separated and identified with this method, especially trans C18 isomers. This method was confirmed to be high throughput and have high sensitivity and a low detection limit. It was successfully applied to separate and identify fatty acids in fresh basil seed oil. The linolenic acid content (51.67 g/100 g) was highest in basil seed oil, followed by linoleic acid (20.16 g/100 g) and oleic acid (12.70 g/100 g). These unsaturated fatty acids in fresh basil seed oil were transformed into trans fatty acids, conjugated linoleic acids and saturated fatty acids after heating to a certain temperature for an adequate duration, and the formation mechanisms were characterised.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Ahmed M. Senan,Sicheng Zhang,Miao Zeng,Zhuqi Chen,Guochuan Yin
Utilization of renewable biomass to partly replace the fossil resources in industrial applications has attracted attention due to the limited fossil feedstock with the increased environmental concerns. This work introduced a modified Wacker-type oxidation for transformation of unsaturated fatty acids/esters to the corresponding keto fatty acids/esters, in which Cu2+ cation was replaced with common nonredox metal ions, that is, a novel Pd(II)/Lewis acid (LA) catalyst. It was found that adding nonredox metal ions can effectively promote Pd(II)-catalyzed oxidation of unsaturated fatty acids/esters to the corresponding keto fatty acids/esters, even much better than Cu2+, and the promotional effect is highly dependent on the Lewis acidity of added nonredox metal ions. The improved catalytic efficiency is attributed to the formation of heterobimetallic Pd(II)/LA species, and the oxidation mechanism of this Pd(II)/LA catalyst is also briefly discussed.
研究领域:
化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
METABOLIC ENGINEERING
·
2020
·
IF 7.263
Xi Deng,Liuqing Chen,Mohan Hei,Tiangang Liu,Yan Feng,Guang-Yu Yang
Medium-chain fatty acids (C6–C10) have attracted much attention recently for their unique properties compared to their long-chain counterparts, including low melting points and relatively higher carbon conversion yield. Thioesterase enzymes , which can catalyze the hydrolysis of acyl-ACP (acyl carrier protein) to release free fatty acids (FAs), regulate both overall FA yields and acyl chain length distributions in bacterial and yeast fermentation cultures. These enzymes typically prefer longer chain substrates. Herein, seeking to increase bacterial production of MCFAs, we conducted structure-guided mutational screening of multiple residues in the substrate-binding pocket of the E. coli thioesterase enzyme ‘TesA. Confirming our hypothesis that enhancing substrate selectivity for medium-chain acyl substrates would promote overall MCFA production, we found that replacement of residues lining the bottom of the pocket with more hydrophobic residues strongly promoted the C8 substrate selectivity of ‘TesA. Specifically, two rounds of saturation mutagenesis led to the identification of the ‘TesA RD−2 variant that exhibited a 133-fold increase in selectivity for the C8-ACP substrate as compared to C16-ACP substrate. Moreover, the recombinant expression of this variant in an E. coli strain with a blocked β -oxidation pathway led to a 1030% increase in the in vivo octanoic acid (C8) production titer. When this strain was fermented in a 5-L fed-batch bioreactor , it produced 2.7 g/L of free C8 (45%, molar fraction) and 7.9 g/L of total free FAs, which is the highest-to-date free C8 titer to date reported using the E. coli type II fatty acid synthetic pathway. Thus, reshaping the substrate binding pocket of a bacterial thioesterase enzyme by manipulating the hydrophobicity of multiple residues altered the substrate selectivity and therefore fatty acid product distributions in cells. Our study demonstrates the relevance of this strategy for increasing titers of industrially attractive MCFAs as fermentation products.
研究领域:
生物化学
·
合成生物学
·
代谢工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CEREAL SCIENCE
·
2021
·
IF 3.616
Pengliang Li,Wei Zhao,Yingying Liu,Aixia Zhang,Songyan Liu,Rui Song,Mengxue Zhang,Jingke Liu
The oxidation of fatty acids affects the aroma of foxtail millet porridge. However, the relationship between volatile compounds and fatty acid precursors is still unclear. Gas chromatography-mass spectrometry was used to detect the volatiles of foxtail millet porridge. The results showed that 23 volatiles were detected, and the content of most aldehydes increased after cooking. The free fatty acid content increased 0.8–1.2-fold, while the total fatty acid content decreased 0.1–0.4-fold. Modeling experiments showed that palmitic acid had no contribution to the formation of volatile compounds, whereas stearic acid produced heptadecane by decarboxylation. Moreover, oleic acid produced octanal , nonanal , nonane, and decanal . Linoleic acid produced hexanal , (Z)-2-heptenal, (E)-2-octenal, 2-pentyl-furan, 3-ethyl-2-methyl-1,3-hexadienal, (E,E)-2,4-decadienal, N-phenyl-benzenamine, 2-n-butylfuran, and benzaldehyde . Linolenic acid produced (E,E)-2,4-decadienal, decanal, benzaldehyde, tetradecane, hexadecane, 2,6,10-trimethyl-pentadecane, and heptadecane. Unsaturated fatty acids play important roles in the formation of the volatiles of foxtail millet porridge.
研究领域:
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING & TECHNOLOGY
·
2018
·Vol. 42
·期 1
·174-181
·
IF 1.588
Fangxia Yang,Junhua Zhang,Yingjie Guo,Lele Feng,Shijun Wei,Shuai Chen
Three Brønsted acidic imidazole dicationic ionic liquids (ILs) with different length of alkyl chains, [C n (Mim) 2 ][HSO 4 ] 2 ( n = 3, 6, 12), were prepared and used as catalyst for the esterification reaction of free fatty acids and methanol. Taking oleic acid as model acid, the catalytic performances of the synthesized ILs for the esterification were evaluated. The main physicochemical properties of the ILs, thermal stability, acidity, solubility in common solvents, and causticity on Austenitic stainless steel 316, were examined. [C 3 (Mim) 2 ][HSO 4 ] 2 demonstrated the highest catalytic activity and enabled to assess the preliminary optimum esterification condition of oleic acid and methanol. Under optimized reaction conditions, the yield of oleic acid methyl ester was up to 95 %. The ILs have great potential as catalysts for producing fatty acid methyl esters from long-chain free fatty acids.
研究领域:
化学工程
·
可再生能源
·
催化科学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Composites and Hybrid Materials
·
2022
·Vol. 5
·期 2
·1268-1279
·
IF 11.806
Su Ji-Ling,Wang Juan,Fu Xiao-Na,Hou Li,Zhu Ping,Zhang Kai,Li Huo-Wang,Hou Ying,Liu Xiang-Yi,Xu Juan
This study explored the physicochemical properties, fatty acid compositions, and thermal characteristics of Malania oleifera seed oil (MOSO). Our data showed that the refractive index and specific gravity of MOSO at ambient temperature were 1.461 and 0.913, respectively. The oil content, acid, peroxide, saponification, iodine value, average molecular weight, and an average number of carbon–carbon double bond per triglyceride were 47.8%, 3.62 mg KOH/g oil, 4.61 meq O 2 /kg oil, 163.16 mg KOH/g oil, 104.28 g I 2 /100 g oil, 1031.51 g/mol, and 4.24, respectively. The fatty acid profiles of the methyl esters showed the presence of 96.09% unsaturated fatty acids and 2.16% saturated fatty acids. Nervonic acid (NA) was the major component (45.04%). These results suggested that MOSO is an excellent source of essential fatty acids and NA and may be used as a potential NA resource of health supplements and medicines. Graphical abstract
研究领域:
植物学
·
食品科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）