INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Junjie Hu,Chaozhang Huang,Anda Gong,Huanle Chen,Shilin Liu,Bin Li,Yan Li
In the present study, we prepared cinnamaldehyde (CA) conjugated chitosan-stabilized emulsions (CSCAEs), and the influence of pH on their properties and lipolysis behavior was investigated. Compared to the emulsions stabilized by chitosan itself (CSEs), CSCAEs had better stability against pH during storage. As pH increased from 2.5 to 6.25, the viscosity and modulus of CSCAEs increased. However, when pH shifted from 6.25 to 6.5, emulsion showed lower viscosity and modulus. Confocal laser scanning microscopy results demonstrated that oil droplets still kept intact and individually distributed, and chitosan homogenously covered on the oil droplets at low pH conditions (pH 2.5, 4.0). At higher pH conditions (pH 5.0, 5.5), a few chitosan aggregates on the oil droplets were observed. At pH 6.0 and 6.25, the compact gel network structure was formed. At pH 6.5, some void was found among the chitosan gel network. In vitro simulated digestion experiments presented that pH had no significant effect on the lipolysis process of emulsions. When the oil content varied from 10% to 50%, emulsions still kept good stability against pHs, but the lipolysis extent after digestion decreased. This knowledge provides a strategy for improving stability of chitosan-stabilized emulsion against harsh pH conditions.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
CARBOHYDRATE POLYMERS
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2017
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IF 4.811
Chun Chen,Qiang Huang,Li-Jun You,Xiong Fu
Three polysaccharides (MFP-1, MFP-2, and MFP-3) were fractioned from mulberry fruits with 30%, 60%, and 90% (v/v) ethanol, respectively. The MFP-1 had the largest molecular weight of 838 kDa, followed by MFP-2 from 49.5 to 163 kDa, and then MFP-3 from 1.41 to 15.30 kDa. The three polysaccharides were composed of arabinose, galactose, glucose, mannose, xylose, and galacturonic acid but with different molar ratio. They also exhibited different surface morphology and thermogravimetric properties. In addition, the impact of the three polysaccharides on the digestion of lipid in a simulated saliva and gastrointestinal tract was investigated. The results indicated MFP-2 had the strongest ability to reduce the rate and extent of lipid digestion. These results have important implications for understanding the influence of polysaccharides on lipid digestion. The effect on retarding lipid digestion within the gastrointestinal tract might be important for the development functional food rich in oil to promote health.
研究领域:
食品科学
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营养学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2016
·
IF 3.858
Chun Chen,Bin Zhang,Xiong Fu,Li-Jun You,Arshad Mehmood Abbasi,Rui Hai Liu
The digestibility of mulberry fruit polysaccharides (MFPs) and its effect on lipid digestion were investigated in the simulated saliva, gastric and intestinal model. Results showed that the saliva, especially the salivary amylase had no effect on MFPs, while the gastric juice had greater effects on the MFPs digestion than intestinal juice, which could be evidenced from the decrease in molecular weight (Mw) from 128.7 ± 6.1, 13.6 ± 1.4 and 2.9 ± 0.1 to 84.3 ± 4.7, 5.2 ± 0.1 and 1.2 ± 0.1 KDa, respectively. It was observed that the amount of reducing ends were increased from 0.051 ± 0.003 to 0.451 ± 0.011 mM and no monosaccharide was released from the polysaccharide during the whole digestion period, indicating that gastrointestinal digestion cleaves the glycosidic bonds and produces no free monosaccharide. In addition, under the simulated saliva, gastric and intestinal digestion conditions, MFPs could reduce the rate and extent of lipid digestion in a concentration-dependent manner. Our findings provide information on the digestibility of polysaccharides from Morus nigra L. in vitro .
研究领域:
食品科学
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营养学
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生物化学
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产品关联
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 1
·175
·
IF 4.7
Danhong Zheng,Weiyan Guan,Jiaqing Chen,Cuicui Zeng,Shen Tan,Jing Chen,Da Ma
Different structural composition ratios of sucrose stearates with hydrophilic–hydrophobic balance (HLB) values ranging from 1 to 16 on lipolysis in emulsion were investigated using a simulated gastrointestinal tract (GIT). Results showed a direct correlation between the HLB values of sucrose stearates and the lipolysis rate of emulsions, and a lower HLB value led to diminished lipolysis in the GIT simulation model. Mechanism study indicated that poor emulsifying capacity of sucrose stearates and lipolysis of sucrose stearates with lower HLB value inhibited the digestive behavior of oil. In addition, monoester was mainly hydrolyzed in the gastric phase, whereas sucrose polyesters caused lipolysis in the intestinal phase using an in vitro digestive model and HPLC analysis, further suppressing lipid digestion. Furthermore, a decrease in cell cytotoxicity and proinflammatory effects on Caco-2 and Raw264.7 were observed post-digestion, respectively. This work offers important insights into the effects of the degree of esterification of sucrose stearate on lipid digestion behavior in oil-in-water emulsions.Keywords:sucrose stearate;digestion behavior;lipolysis;bioactivity;GI tract
研究领域:
食品科学
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营养学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Biao Liu,Ye-hui Liang,Yang-Zheng He,Wenhui Ye,Ze-Yuan Deng,Jing Li,Shuntang Guo
The differences in the milk fat digestion from goat milk (GM), camel milk (CM), bovine milk (BM), sheep milk (SM), mare milk (MM) and human milk (HM) using an in vitro gastrointestinal digestion model for simulated infants were investigated. The particle size distributions in goat and mare milk were similar to that of HM after digestion in the small intestine. During in vitro digestion, the zeta-potential change of MM was more consistent with that of HM. After 60 min of gastric digestion, the lipolysis degree (LD) of different milks were<2%, of which the highest LD was MM (1.84%), followed by HM (1.45%). At the end of intestinal digestion, the LD of HM was the highest, reaching 88.47%, and the LD of SM was similar to that of HM, reaching 83.92%, followed by GM (57.00%), BM (40.98%) and MM (39.37%), respectively, the LD of CM was only 29.99%, which was much lower than HM. The results of the glyceride composition hierarchical clustering analysis revealed that MM and HM were clustered into one category at the end of gastric and intestinal digestion. This study provides a scientific basis for the development of lipid ingredients in infant formula.
研究领域:
营养学
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食品科学
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儿科医学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2016
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IF 4.712
Wen-Qi Chang,Jian-Liang Zhou,Yi Li,Zi-Qi Shi,Li Wang,Jie Yang,Ping Li,Li-Fang Liu,Gui-Zhong Xin
The elevation of free fatty acids (FFAs) has been regarded as a universal metabolic signature of excessive adipocyte lipolysis . Nowadays, in vitro lipolysis assay is generally essential for drug screening prior to the animal study. Here, we present a novel in vitro approach for lipolysis measurement combining UHPLC-Orbitrap and partial least squares (PLS) based analysis. Firstly, the calibration matrix was constructed by serial proportions of mixed samples (blended with control and model samples). Then, lipidome profiling was performed by UHPLC-Orbitrap, and 403 variables were extracted and aligned as dataset. Owing to the high resolution of Orbitrap analyzer and open source lipid identification software, 28 FFAs were further screened and identified. Based on the relative intensity of the screened FFAs, PLS regression model was constructed for lipolysis measurement. After leave-one-out cross-validation, ten principal components have been designated to build the final PLS model with excellent performances (RMSECV, 0.0268; RMSEC, 0.0173; R 2 , 0.9977). In addition, the high predictive accuracy ( R 2 = 0.9907 and RMSEP = 0.0345) of the trained PLS model was also demonstrated using test samples. Finally, taking curcumin as a model compound, its antilipolytic effect on palmitic acid-induced lipolysis was successfully predicted as 31.78% by the proposed approach. Besides, supplementary evidences of curcumin induced modification in FFAs compositions as well as lipidome were given by PLS extended methods. Different from general biological assays , high resolution MS-based method provide more sophisticated information included in biological events. Thus, the novel biological evaluation model proposed here showed promising perspectives for drug evaluation or disease diagnosis.
研究领域:
代谢组学
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药物研发
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分析化学
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产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-19
·
IF 15.7
Peng Han,Xu Qikui,Zhang Ting,Zhu Jiakai,Pan Jinheng,Guan Xiaoyu,Feng Shan,Wu Jianping,Hu Qi
Lipid droplets (LDs) are the main cellular storage sites for triacylglycerols (TAGs), playing an important role in energy homeostasis and cell signaling. Hydrolysis of the stored TAGs begins with conversion of TAGs into diacylglycerols (DAGs) by adipose triglyceride lipase (ATGL), followed by hydrolysis of DAGs by hormone-sensitive lipase (HSL). Despite the central role of HSL in lipolysis, the molecular determinants for its LD association have remained elusive. Here, we report the cryo-EM structure of human HSL at 3.4 Å. Combining this with hydrogen-deuterium exchange mass spectrometry, biochemical and cellular assays, we identify residues 489-538, referred to as the “H-motif”, and the N-terminal 4-helix bundle of HSL as LD-binding motifs mediating direct interaction of HSL with LDs. LD binding mediated by the LD-binding motifs is independent of HSL phosphorylation catalyzed by the cAMP-dependent kinase PKA. Our findings provide insight into the LD binding mechanism of HSL, advancing our understanding of the regulation of lipolysis.
研究领域:
分子生物学
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细胞生物学
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结构生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2018
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IF 3.412
Ling-Zhi Cheong,Chenyu Jiang,Xiaoqian He,Shuang Song,Oi-Ming Lai
Dairy technologists has attempted to produce “improved” infant formulas mimicking human milk by supplementation with bovine MFGM and/or phospholipids-enriched materials. The present study investigated and compared the lipid profile and particle sizes of mature human milk and infant formula fat globules (IF 1, IF 2, IF 3, and IF 4) and elucidated the relationship between physicochemical properties and in vitro simulated gastrointestinal lipolysis rate of the different milk samples. Despite having larger micron-sized fat globules, mature human milk demonstrated the highest gastrointestinal lipolysis rate with higher release of medium- and long-chain saturated fatty acids. In comparison, IF 3, which contained the lowest phospholipids content, demonstrated the lowest gastrointestinal lipolysis rate. Higher gastrointestinal lipolysis rate of mature human milk fat as compared to infant formula fats might be due to the presence of MFGM interfacial layer (phospholipids) surrounding the fat droplets which govern lipase activity on lipid droplets.
研究领域:
营养学
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食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Yue Sun,Bin Zhu,Pingyao Cong,Xiaodong Li,Lu Liu,Chanchan Guo,Kuangyu Zhao,Jiaxin Qiu,Haowen Ji,Xiaojun Zhu,Kouadio Jean Eric-Parfait Kouame
ABSTRACT The digestive behavior and absorption properties of lipids are affected by differences in the interface structures of fat globules in human milk (HM) and infant formula. However, it is not clear how the interface changes of fat globules further regulate lipid uptake by improving lipid digestion. Based on the milk fat globule membrane (MFGM) and milk protein, two infant formula emulsions with different interface structures (F1: MFGM-stabilized emulsion, F2: whey protein and casein-stabilized emulsion) were prepared to simulate HM fat globules. The interface structure of lipid droplets, digestion behavior and absorption characteristic of lipids in infant formula emulsions were evaluated and compared with those of HM and commercial infant formula (IFM: supplied with MFGM). The results showed that MFGM-stabilized fat globules in F1 had a similar interface structure to HM. F1 could alleviate the coalescence and aggregation of lipid and protein in comparison with F2 and IFM. The order of triglyceride secretion from high to low was: HM (51.63 ± 2.0 μmol/L), F1 (45.19 ± 1.7 μmol/L), F2 (35.28 ± 1.3 μmol/L) and IFM (32.23 ± 1.3 μmol/L), indicating that HM and F1 exhibited faster lipid digestion and more chylomicron secretion. The expression of SLC27A4, FABP2, SCARB1, DGAT2, PLIN2, and MOGAT2 in HM and F1 was significantly higher than in F2 and IFM. Altogether, the simulation of HM fat globule at the interface structure based on MFGM could better promote cellular lipid uptake by influencing the digestion behavior of fat globules.
研究领域:
食品科学
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营养学
·
儿科
✓ 已验证
DOI
高影响因子
PHYTOMEDICINE
·
2024
·
IF 6.7
Huijian Chen,Pengxin Liu,Ruitao Yu,Nabijan Mohammadtursun,Ainiwaer Aikemu,Xinzhou Yang
Background and purpose Swertiamarin (STM), a secoiridoid glycoside from Swertia chirayita (Roxb.) H. Karst , has been shown to decrease body weight, blood glucose, and blood lipids by inhibiting adipose tissue hypertrophy. However, the underlying mechanisms remain unclear. In particular, adipose thermogenesis is a novel avenue for exploring the pharmacological effects of STM. We aim to investigate the efficacy of STM on type 2 diabetes mellitus (T2DM), with a focus on underlying mechanisms, particularly the activation of ADRB3/UCP1 thermogenic signaling pathways. Methods T2DM model was established by a high-fat diet (HFD) and streptozotocin (STZ) in C57BL/6 J male mice. Mice were given to either 100 or 200 mg kg -1 /day of STM, or 200 mg kg -1 /day of metformin (Glucophage) via intragastric administration for 7 weeks. In vitro, 3T3-L1 cells were differentiated into adipocytes. Molecular markers related to ADRB3-UCP1 signals, lipolysis, and mitochondrial function were detected. Results STM-treated diabetic mice showed a reduction of body weight, fat mass, and blood glucose/lipids and an improvement in insulin sensitivity. Bioinformatics analysis indicated STM promoted lipid metabolism and mitochondrial function, features by closely associated with adipose thermogenesis. STM upregulated the lipolysis-related genes and p-HSL protein in inguinal subcutaneous white adipose tissue (igSWAT) and brown adipose tissue (BAT). STM-treated mice processed a more active energy metabolism. Additionally, the ADRB3-UCP1 signals, mitochondrial-related genes, and oxidative phosphorylation were improved in igSWAT and BAT. In vitro , we found STM interacted with ADRB3, increasing glucose uptake, glycerol release, ADRB3-UCP1 signals, p-HSL expression, mitochondrial content, oxidative phosphorylation complex expression with improved mitochondrial Δ ψ m, as well as reduced lipid accumulation in adipocytes. All these effects were reversed upon ADRB3 inhibition. Conclusion This study identifies a previously unknown role of STM activating ADRB3/UCP1 signals in adipose tissue, suggesting a potential strategy for treating T2DM.
研究领域:
药理学
·
内分泌学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Pharmaceutics
·
2023
·Vol. 15
·期 5
·1341
·
IF 5.4
Yuze Sheng,Qin Yu,Yanping Huang,Quangang Zhu,Zhongjian Chen,Wei Wu,Tao Yi,Yi Lu
Nanocrystals (NCs) have the potential to enhance the oral bioavailability of Class IV drugs in the Biopharmaceutical Classification System (BCS) due to the absorption of the intact crystals. The performance is compromised by the dissolution of NCs. Drug NCs have recently been adopted as solid emulsifiers to prepare nanocrystal self-stabilized Pickering emulsions (NCSSPEs). They are advantageous in high drug loading and low side effects due to the specific drug loading mode and the absence of chemical surfactants. More importantly, NCSSPEs may further enhance the oral bioavailability of drug NCs by impeding their dissolution. This is especially true for BCS IV drugs. In this study, curcumin (CUR), a typical BCS IV drug, was adopted to prepare CUR-NCs stabilized Pickering emulsions using either indigestible (isopropyl palmitate, IPP) or digestible (soybean oil, SO) oils, i.e., IPP-PEs and SO-PEs. The optimized formulations were spheric with CUR-NCs adsorbed on the water/oil interface. The CUR concentration in the formulation reached 20 mg/mL, which was far beyond the solubility of CUR in IPP (158.06 ± 3.44 μg/g) or SO (124.19 ± 2.40 μg/g). Moreover, the Pickering emulsions enhanced the oral bioavailability of CUR-NCs, being 172.85% for IPP-PEs and 152.07% for SO-PEs. The digestibility of the oil phase affected the amounts of CUR-NCs that remained intact in lipolysis and, thus, the oral bioavailability. In conclusion, converting NCs into Pickering emulsions provides a novel strategy to enhance the oral bioavailability of CUR and BCS IV drugs.Keywords:curcumin;nanocrystals;Pickering emulsions;oral bioavailability;BCS IV;lipolysis
研究领域:
药剂学
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生物医学工程
·
药物代谢
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产品关联
DOI
PubMed
高影响因子
EMBO REPORTS
·
2020
·Vol. 21
·期 11
·e50214
·
IF 7.497
Leilei Yang,Jingjing Liang,Sin Man Lam,Ahmet Yavuz,Guanghou Shui,Mei Ding,Xun Huang
Lipid droplets (LDs) are dynamic cytoplasmic organelles present in most eukaryotic cells. The appearance of LDs in neurons is not usually observed under physiological conditions, but is associated with neural diseases. It remains unclear how LD dynamics is regulated in neurons and how the appearance of LDs affects neuronal functions. We discovered that mutations of two key lipolysis genes atgl-1 and lid-1 lead to LD appearance in neurons of Caenorhabditis elegans. This neuronal lipid accumulation protects neurons from hyperactivation-triggered neurodegeneration, with a mild decrease in touch sensation. We also discovered that reduced biosynthesis of polyunsaturated fatty acids (PUFAs) causes similar effects and synergizes with decreased lipolysis. Furthermore, we demonstrated that these changes in lipolysis and PUFA biosynthesis increase PUFA partitioning toward triacylglycerol, and reduced incorporation of PUFAs into phospholipids increases neuronal protection. Together, these results suggest the crucial role of neuronal lipolysis in cell-autonomous regulation of neural functions and neurodegeneration.
研究领域:
神经科学
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代谢生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）