FOOD CHEMISTRY
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2022
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IF 9.231
Zhiqing Liu,Mantong Zhao,Xinwen Wang,Chuan Li,Zhongyuan Liu,Xuanri Shen,Dayong Zhou
The present study sought to analyze the lipid profiles of oyster Crassostrea gigas from Yellow Sea (YS), East China Sea, and South China Sea (SCS) through the untargeted lipidomics strategy based on UPLC-Q-Exactive Orbitrap mass spectrometry and multivariate statistics. The results elucidated that geographical differences had profound effects on the lipid content, composition, and lipid molecular profiles. Notably, oysters from the YS group contained the highest lipid content, including triacylglycerol, diacylglycerols, and the majority of phospholipid molecule species, while oysters from the ECS group contained most of the phosphatidylcholine species and the SCS group contained most of the sphingolipid species. Totally, 1155 lipid molecular species belonging to 21 subclasses were identified; of them, 45 lipid molecular species could serve as differential marker for lipid of oysters from different sea areas. Overall, lipidomics could be a potential approach for discrimination of lipid characters between marine shellfishes for geographical origin traceability.
研究领域:
海洋生物学
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分析化学
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食品科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
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2024
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IF 8.5
Qiuhui Xu,Jie Wang,Dan Wang,Xin Lv,Li Fu,Ping He,Desheng Mei,Hong Chen,Fang Wei
Rapeseed ( Brassica napus L. ) is the second largest globally cultivated oil crop, but the effects of post-harvested ripening on rapeseed quality is unclear and unpredictable. This study reveals the relationship between post-harvest ripening periods (PHR) and physicochemical quality of different rapeseed cultivars using comprehensive physicochemical indicators analysis. The results indicate that PHR led to a gradual decrease in chlorophyll, carotenoid and moisture content but continually increased oil and total phenol content (TPC). Besides, 295 lipid molecules from 13 lipid subclasses were identified, revealing that the relative content of triacylglycerol (TG) was progressively increased while diacylglycerol (DG) demonstrated a consistent decline throughout the PHR. Correlation analysis, hierarchical cluster analysis (HCA) and principal component analysis (PCA) were employed to construct and verify the comprehensive quality evaluation model for rapeseeds in PHR. This paper develops a comprehensive quality evaluation model for post-harvest ripening rapeseeds and advances the development of agricultural products.
研究领域:
植物学
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食品科学
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农学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2021
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IF 10.588
Qianyu Chen,Yayun Zhang,Jianhua Li,Guanyong Su,Qi Chen,Zhen Ding,Hong Sun
Exposure to neonicotinoid insecticides (NNIs) was proven to be harmful to organisms, however, there is a dearth of information regarding their occurrence and adverse effects on the general residents. Here, n = 120 human serum samples were collected from the same area of Wuxi city, Eastern China, and these serum samples were further analyzed for nine NNIs and nine target lipid molecules by use of ultrahigh performance liquid chromatography-quadrupole orbitrap high-resolution mass spectrometer . We observed that four out of nine NNIs exhibited relatively high detection frequencies (DF), and these NNIs were imidacloprid (IMI; DF = 28.3%), clothianidin (CLO; 16.7%), thiacloprid (THI; 14.2%), and acetamiprid (ACE; 12.5%), respectively, with 95th concentrations ranging from 32.0 to 427 pg/mL. Median concentrations of imidacloprid-equivalent total neonicotinoids (IMI eq ) and ∑ 7 NNI were 46.6 pg/mL and 26 pg/mL, respectively. Five out of nine lipid molecules exhibited higher levels, that were docosahexaenoic acid [FA(22:6)], 18:0 phosphocholine [LysoPC(18:0)], 18:0 phosphoethanolamine [LysoPE(18:0)], D18:1–18:0 sphingomyelin [SM(d18:1/18:0)], and 18:1–18:1 diglycerol [DG(18:1/18:1)], respectively. More interestingly, we observed statistically significant correlations (student’s t -test, one-way ANOVA, or Mann-Whitney test; p < 0.05) between NNI levels and population characteristics (i.e. age, smoking, and health status). Beyond that, we also observed statistically significant correlations between levels of selected NNIs (CLO, ACE, or THI) and lipid molecules [LysoPE(18:0), SM(d18:1/18:0), and DG(18:1/18:1)]. Collectively, for the first time, we provided the information on contamination levels of NNIs in serum samples of general residents in China and demonstrated the associations between concentrations of NNIs and levels of lipid molecular species.
研究领域:
环境科学
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公共卫生
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2022
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IF 4.52
Jingtao Cui,Jun Cao,Jiachen Ge,Xingchen Qu,Pengyan Li,Chuan Li
Cobia ( Rachycentron canadum ) is a significant fish species for mariculture with high nutritional value . This research aims to comprehensively investigate the lipid profiles of cobia tissues, including fatty acid profiles, characteristics of phospholipids (PL) and triacylglycerols (TAG), and lipid molecular species. The results showed that cobia contained high levels of total lipids (2.89 −32.09 % of wet weight), docosahexaenoic acid (DHA, 10.83 −14.69 % of total fatty acids) and eicosapentaenoic acid (EPA, 3.54 −9.68 %). Compared with TAG, higher levels of polyunsaturated fatty acids , DHA/EPA in particular, were concentrated in PL. Interestingly, DHA was predominantly distributed in the sn-1 position of PL and the sn-2 position of TAG, whereas EPA was predominantly distributed in the external positions of PL and TAG. Additionally, at least 693 molecular species from 23 subclasses were identified by ultrahigh-performance liquid chromatography-quadrupole exactive-mass spectrometry (UPLC-Q-Exactive-MS). Glycerophospholipids (63.19 −66.61 %) and glycerolipids (19.17 −22.21%) were the major lipid classes in cobia tissues, and the majority of them contained DHA and EPA. Hence, this comprehensive analysis will help to evaluate the lipid nutritional values and facilitate exploitation in cobia consumption and processing.
研究领域:
水产养殖学
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营养学
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脂质组学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
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2024
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IF 7
Jingtao Cui,Tong Li,Yanchi Zhou,Liling Wang,Tian Li,Weimin Zhang
While tropical fruit seeds are considered potential sources of functional or edible vegetable oils, their lipid profiles are poorly documented. Herein, the lipid profiles of nine tropical fruit seed oils were systematically evaluated and compared using lipidomics and chemometrics techniques. Cherimoya exhibited the highest total lipid content, while avocado had the lowest. Canistel, cherimoya, and durian displayed a 9cC18:1 predominance. The remaining six seed oils were dominated by 9c12cC18:2n-6. In total, 1370 lipid molecules were identified, with triacylglycerol being the predominant subclass. Passion fruit, cherimoya, and durian had the highest glycerolipid, sphingolipid, and fatty acyl contents, respectively. Litchi exhibited the highest levels of glycerophospholipid and saccharolipid. Chemometric modeling screened 134 differential lipid molecules as markers for distinguishing between various tropical fruit seed oils. Positive correlations were primarily observed among the differential lipid molecules. Overall, these findings provide valuable insights into the integrated utilization of tropical fruit seed oils.
研究领域:
植物学
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食品科学
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代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
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2024
·
IF 8.1
Yitong Yan,Jin Qian,Yin Liu,Jing Hu,Bianhe Lu,Shasha Zhao,Shuai Jin,Yuxuan He,Kailin Xu
Triclocarban (TCC), an emerging contaminant in water environments, its effects on freshwater biofilms remain insufficiently understood. This study investigates the effects of TCC exposure (at concentrations of 10 μg L −1 and 10 mg L −1 ) on mature freshwater biofilms. TCC was found to inhibit biofilm activity as evidenced by changes in surface morphology and the ratio of live/dead cells. Moreover, both concentrations of TCC were observed to modify the structure of the biofilm community. Metabolomics analysis revealed an overlap in the toxicity mechanisms and detoxification strategies triggered by various concentrations of TCC in biofilms. However, the higher toxicity induced by 10 mg L −1 TCC resulted from the downregulation of proline betaine , disrupting the homeostasis of cellular osmotic pressure regulation in biofilms. Notably, lipid and lipid-like molecules showed high sensitivity to different concentrations of TCC, indicating their potential as biomarkers for TCC exposure. Annotation of the differential metabolites by KEGG revealed that alterations in amino acid and carbon metabolism constituted the primary response mechanisms of biofilms to TCC. Moreover, the biofilm demonstrated enhanced nucleic acid metabolism , which bolstered resistance against TCC stress and heightened tolerance. Furthermore, elevated TCC concentrations prompted more robust detoxification processes for self-defense. Overall, short-term exposure to TCC induced acute toxicity in biofilms, yet they managed to regulate their community structure and metabolic levels to uphold oxidative homeostasis and activity. This research contributes to a deeper comprehension of TCC risk assessment and policy control in aquatic environments .
研究领域:
环境科学
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微生物学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2019
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IF 5.839
Lechuan Wang,Lisha Li,Nuo Xu,Weiqing Sun,Baomiao Ding,Wei Xu,Zhenshun Li
Lysozyme (Ly), as an important natural preservative, easily interacts with anionic polysaccharides in food , which results in low and unstable bacteriostatic activity. The interaction of carboxymethylcellulose (CMC) with Ly was used to investigate the mechanism of the bioactivity changes caused by Ly binding polysaccharides. The affinity of Ly or Ly-CMC complexes for a mixed monolayer liposome (Lip) as an in vitro biomembrane model was characterized by using dynamic light scattering, Fourier transform infrared spectroscopy , circular dichroism spectroscopy , fluorescence analysis and interface activity measurements. The results showed that the affinity between Ly and Lip mainly depended on hydrophobic and electrostatic forces. The electrostatic interaction in Ly-CMC weakened the Ly-Lip affinity and altered the spatial conformation of Ly molecules. Furthermore, CMC screened the active sites of Ly and neutralized the charge of Ly, which may reduce its bioactivity. The presence of CMC decreased the increase in surface pressure in the Lip-Ly interface, which means that Ly-CMC has a lower insertion capacity and ability to induce lipid molecule rearrangement than Ly alone, indicating an inferior bacteriostatic ability. Our work explains how the bacteriostatic mechanism of Ly is affected by food biopolymers.
研究领域:
食品科学
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生物化学
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生物物理学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
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2023
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IF 7.4
Ling Liu,Ting Zhang,Zheng Wu,Fenghai Zhang,Yunpeng Wang,Xiaotong Wang,Zhe Zhang,Chengming Li,Xinpeng Lv,Deqiang Chen,Songyan Jiao,Jing Wu,Yang Li
The detection of biomolecules is the key to basic molecular research, diagnostics, drug screening, and other biomedical applications. However, the existing detection techniques can only detect single classes of biomolecules, which warrant the development of a versatile biomolecule detection platform. Here, we developed a universal method for label-free detection of biomolecules via surface-enhanced Raman spectroscopy (SERS) by using sulfhydryl-modified gold nanoparticles as the substrate. The biomolecules can be adsorbed on the surface of gold nanoparticles cleaned by bromide ions to obtain initially enhanced Raman signals, and the aggregator (calcium ion) was further added to form a “hot spot”, which enhanced the biomolecular signal again. Through the “two-step enhancement method”, we were able to obtain fingerprints of DNA, RNA, amino acids, peptides, proteins, viruses, bacteria, and lipid molecules. This low-toxic, highly sensitive, and widely applicable technique has potential applications in biomedical research, clinical testing, and disease diagnosis and lays the foundation for the development of SERS technology in various fields.
研究领域:
生物医学工程
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分析化学
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纳米技术
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分子生物学
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诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）