FOOD CHEMISTRY
·
2024
·
IF 8.5
He-He Tian,Yu-Lian Li,Xu-Song Wang,Xu-Hui Huang,Yu-Ying Zhang,Zi-Chun Zhang,Da-Yong Zhou,Lei Qin
Abalone, a highly sought-after aquatic product, possesses significant nutritional value. In this study, the relationship between aroma characteristics and lipid profile of abalone ( Haliotis discus hannai ) during seasonal fluctuation and thermal processing were profiled via volatolomics and lipidomics. 46 aroma compounds and 371 lipids were identified by HS-SPME-GC–MS and UPLC-Q-Extractive Orbitrap-MS, respectively. Multivariate statistical analysis indicated that carbonyls (aldehydes and ketones) and alcohols were the characteristic aroma compounds of abalone. The fluctuations in the aroma compound and lipid composition of abalone were consistent with the seasonal variation, especially seawater temperature. In addition, based on the correlation analysis, it was found that carbonyls (aldehydes and ketones) and alcohols had a positive correlation with phospholipids (lysophosphatidylethanolamines and lysophosphatidylcholines), while a negative correlation was observed with fatty acyls. These findings suggested that the effect of seasonal variations on the aroma changes of abalone might achieved by modulating the lipids composition of abalone.
研究领域:
食品科学
·
生物化学
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DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Jingtao Cui,Li Cui,Tao Zhang,Xinchen Jiang,Yaming Qian,Wuyang Huang,Haijun Zhu
This study aimed to evaluate the impact of conventional (pressing extraction [PSE], solvent extraction [STE]), and non-conventional (microwave-assisted solvent extraction [MSE], subcritical fluid extraction [SBFE], supercritical fluid extraction [SPFE]) techniques on the quality of pecan oil using foodomics. The results indicated that MSE, STE, and SBFE yielded higher oil quantities. SPFE selectively extracted free fatty acids, leading to a higher acid value. MSE-derived oil exhibited the highest oxidative stability index. A total of 844 lipid molecules were identified, with glycerolipids and glycerophospholipids as the predominant classes. MSE and SBFE showed superior efficiency over conventional methods for the extraction of glycerophospholipids and saccharolipids. Additionally, 48 volatile compounds were identified, predominantly aldehydes and esters, with seven recognized as key aroma contributors. Dimethyl trisulfide had the most significant impact on the overall aroma. These findings offer a theoretical foundation for the efficient and sustainable production of pecan oil.
研究领域:
食品科学
·
植物化学
·
分析化学
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DOI
PubMed
高影响因子
Journal of Aquatic Food Product Technology
·
2020
·
IF 1.02
Yan-Fei Liu,Zi-Xuan Wu,Jing Zhang,Yu-Xin Liu,Zhong-Yuan Liu,Hong-Kai Xie,Kanyasiri Rakariyatham,Da-Yong Zhou
Lipid profile of the oyster Crassostrea talienwhanensis from the Yellow Sea was evaluated based on the seasonal changes in lipid categories, phospholipid (PL) categories, fatty acid (FA) composition, atherogenicity index (IA), thrombogenicity index (IT), and glycerophospholipid (GP) molecular species. We observed that the samples harvested in February contained higher levels of polyunsaturated fatty acid (PUFA) (48.77% of total FAs) and PL (52.12% of total lipids) and had lower IA (0.29). In addition, more than 290 GP molecular species of glycerophosphocholine (GPCho), glycerophosphoethanolamine (GPEtn), glycerophosphoserine (GPSer), and glycerophosphoinositol (GPIns) were determined. The molecular species containing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) constituted 75.24% of total GPCho in the samples harvested in February. Considering the lipid profile, oyster Crassostrea talienwhanensis harvested in February had better nutritional value.
研究领域:
营养学
·
海洋生物学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Tao Wang,Xiaoming Chen,Chunyan Luan,Jianmin Wu
Cell heterogeneity of tumor tissues is one of the causes of cancer recurrence after chemotherapy. Cell subtype identification in tumor tissues of specific cancer is critical for precision medicine and prognosis. As the structural and functional components of cells, lipids are closely related to the apparent morphology of cells. They are potential biomarkers of species of cancers and can be used to classify different cancer cell types, but it remains a challenge to establish a stable cell differentiation model and extend it to tumor tissue cell subtype differentiation. Here we describe a lipid profiling method based on nanostructure assisted laser desorption/ionization mass spectrometry (NALDI-MS), which could classify five hepatocellular carcinoma (HCC) cell lines and discriminate subtype of mixed cells and tumor tissues. The NALDI target was patterned with array of sample spots containing vertical silicon nanowires (Si NWs). Owing to its high ability to absorb laser energy, the vertical Si NWs can help to generate abundant lipid ions of cell extracts without need of organic matrix. Combined with statistical analysis methods, twenty-two ion peaks distributed in four MS peak clusters were selected as potential biomarkers to distinguish the subtype of the five HCC cell lines. Peak normalization was performed within each MS peak cluster to reduce the variation of peak intensity in batch to batch analysis. Compared to full-spectrum normalization method, the inner-cluster normalization method could help to distinguish cell subtype more stably and accurately. The molecular structure of these biomarkers was identified and sorted into two classes including phosphatidylcholine (PE, PI, PG, PA, PS) and glycosphingolipid (LacCer, ST). Furthermore, the established method was successfully applied to identify the major HCC cell subtype in mixed cell samples and xenograft tumor tissues as well as drug response test, showing great potential in precision medicine and prognosis.
研究领域:
肿瘤学
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代谢组学
·
精准医学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Ling-Zhi Cheong,Chenyu Jiang,Xiaoqian He,Shuang Song,Oi-Ming Lai
Dairy technologists has attempted to produce “improved” infant formulas mimicking human milk by supplementation with bovine MFGM and/or phospholipids-enriched materials. The present study investigated and compared the lipid profile and particle sizes of mature human milk and infant formula fat globules (IF 1, IF 2, IF 3, and IF 4) and elucidated the relationship between physicochemical properties and in vitro simulated gastrointestinal lipolysis rate of the different milk samples. Despite having larger micron-sized fat globules, mature human milk demonstrated the highest gastrointestinal lipolysis rate with higher release of medium- and long-chain saturated fatty acids. In comparison, IF 3, which contained the lowest phospholipids content, demonstrated the lowest gastrointestinal lipolysis rate. Higher gastrointestinal lipolysis rate of mature human milk fat as compared to infant formula fats might be due to the presence of MFGM interfacial layer (phospholipids) surrounding the fat droplets which govern lipase activity on lipid droplets.
研究领域:
营养学
·
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2018
·Vol. 9
·期 10
·5103-5114
·
IF 3.289
Zongyuan Wu,Zumeng Xia,Bangfu Wu,Fang Wei,Xin Lv,Ya Xie,Shuling Xu,Jiqu Xu,Hong Chen,Fenghong Huang
Atherosclerosis is a chronic disease associated with oxidative stress and inflammatory activation and is the main underlying trigger for cardiovascular disease. There are many cardiovascular health products in the market; in order to evaluate the effect of these products, in this paper, a novel lipid profiling platform was established using the shot-gun mass spectrum method for the analysis of free fatty acid and phospholipids, and the high performance liquid chromatography coupled with mass spectroscopy method for the analysis of lipid mediators and triacylglycerol, respectively, in serum from male apolipoprotein E-knock out mice after different diet interventions. Changes in the four lipids above, and pathways and regulation of lipid metabolism in mice from different groups were further investigated. The result showed that all cardiovascular health products showed some certain potential to alleviate atherogenesis and ameliorate inflammation; among them, lemon apple cider vinegar drink and seal oil could significantly decrease triacylglycerol in mouse serum. The establishment of this lipidome profiling platform helps to better understand the metabolism regulation and intervention mechanism of different cardiovascular health products in chronic diseases, such as atherogenesis. This platform could be applied to other cardiovascular health products and provide reliable lipid profiling data for their potential effect.
研究领域:
生物化学
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代谢组学
·
心血管病学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Yu-Xi Liu,Yu-Ying Zhang,Jie Zheng,Jia-Nan Chen,Xu-Hui Huang,Xiu-Ping Dong,Bei-Wei Zhu,Lei Qin
Scallop is popular for its delicious taste and rich nutrition value. This study investigated the metabolomic changes in free amino acid (FAA), 5′-nucleotide, and lipid composition of scallops collected in different seasons. Results showed that the content of the FAA and 5′-nucleotide in summer was significantly higher than in other seasons. Adenosine 5′-monophosphate, glycine, and alanine were dominant from June to August. A total of 176 lipids, including 50 triacylglycerols (TAG), 60 phosphatidylcholines (PC), and 30 phosphatidylethanolamines (PE), were identified in the scallop adductor muscle. PC and PE were dominant from February to June, and TAG was dominant in November. The unsaturation degree of TAG was increased from July to November, and the highest proportion of polyunsaturated phospholipids appeared in August and September. Overall, scallops have high nutritional value in summer. This study is helpful for the development of scallop products with high nutrition and high value.
研究领域:
海洋生物学
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食品科学
·
代谢组学
✓ 已验证
DOI
高影响因子
Drug Design Development and Therapy
·
2023
·
IF 4.8
Wenming Zhao,Chengwei Yang,Ning Zhang,Yuanyuan Peng,Ying Ma,Keru Gu,Xia Liu,Xiaohui Liu,Xijian Liu,Yumin Liu,Songkai Li,Linjing Zhao
Objective The characteristic constituents of essential oils from aromatic plants have been widely applied as antimicrobial agents in the last decades. However, their mechanisms of action remain obscure, especially from the metabolic perspective. The aim of the study was to explore the antimicrobial effect and mechanism of menthone, a main component of peppermint oil, against methicillin resistant Staphylococcus aureus (MRSA).Methods An integrated approach including the microbiology and the high-coverage lipidomics was applied. The changes of membrane properties were studies by the fluorescence and electron microscopical observations. The lipid profile was analyzed by ultra-high performance liquid chromatography coupled with quadruple Exactive mass spectrometry (UHPLC-QE-MS). The lipid-related key targets which were associated with the inhibitory effect of menthone against MRSA, were studied by network analysis and molecular docking.Results Menthone exhibited antibacterial activities against MRSA, with minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of 3,540 and 7,080 μg/mL, respectively. The membrane potential and membrane integrity upon menthone treatment were observed to change strikingly. Further, lipids fingerprinting identified 136 significantly differential lipid species in MRSA cells exposed to menthone at subinhibitory level of 0.1× MIC. These metabolites span 30 important lipid classes belonging to glycerophospholipids, glycolipids, and sphingolipids. Lastly, the correlations of these altered lipids, as well as the potential metabolic pathways and targets associated with menthone treatment were deciphered preliminarily.Conclusion Menthone had potent antibacterial effect on MRSA, and the mechanism of action involved the alteration of membrane structural components and corresponding properties. The interactions of identified key lipid species and their biological functions need to be further determined and verified, for the development of novel antimicrobial strategies against MRSA.
研究领域:
微生物学
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代谢组学
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药理学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2021
·Vol. 12
·期 22
·11557-11567
·
IF 5.396
Yimin Zhao,Zouyan He,Wangjun Hao,Hanyue Zhu,Jianhui Liu,Ka Ying Ma,Wen-Sen He,Zhen-Yu Chen
The present study aimed to investigate the effects of protocatechuic acid (PCA) on plasma lipid profiles and associated mechanisms with a focus on reshaping gut microbiota. Twenty-four male hamsters were randomly divided into three groups receiving a high-cholesterol diet (HCD) and two HCD diets containing 0.5% (PL) and 1% (PH) PCA, respectively. Feeding PL and PH diets for six weeks significantly reduced plasma total cholesterol by 18% and 24%, respectively. PL and PH diets also significantly lowered plasma non-HDL cholesterol by 37% and 44%, respectively. This was accompanied by an increase in fecal short-chain fatty acids (SCFAs) and fecal bile acids with up-regulation on gene of cholesterol 7α-hydroxylase and down-regulation of 3-hydroxy-3-methylglutaryl-CoA reductase in the liver. Dietary PCA supplementation decreased hepatic lipid accumulation, whereas it increased fecal excretion of lipids. The 16S rRNA analysis found that dietary PCA significantly reduced the ratio of Firmicutes to Bacteroidetes and increased the relative abundance of Bacteroidales S24-7, whereas it reduced the abundance of Lactobacillaceae. It was concluded that dietary PCA favorably modulated plasma lipid profiles and prevented the accumulation of hepatic cholesterol and lipid disposition. Such effect was mediated at least partially by increasing gut production of SCFAs and fecal excretion of bile acids via modulating the gut microbiome.
研究领域:
营养学
·
微生物学
·
生物化学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2017
·
IF 4.529
Xiao-Pei Hu,Qing-Da An,Da-Yong Zhou,Ting Lu,Fa-Wen Yin,Liang Song,Qi Zhao,Jiang-Hua Zhang,Lei Qin,Bei-Wei Zhu,Fereidoon Shahidi
Different classes of lipids and phospholipids (PL) as well as fatty acid (FA) compositions, and glycerophospholipid (GP) molecular species in edible parts (adductor muscles) and by-products (gonads and mantles) of scallops Patinopecten yessoensis and Chlamys farreri were characterized. The lipids contained high levels of polyunsaturated FA (PUFA) (20.44–58.81% of total FAs) and PL (54.99–78.26% of total lipids). Among PL, phosphatidylcholine (41.39–51.86 mol%) was dominant. More than 230 GP species belonging to glycerophosphocholine, glycerophosphoethanolamine, glycerophosphoserine, glycerophosphoinositol, lysoglycerophosphocholine, lysoglycerophosphoethanolamine and lysoglycerophosphoinositol were characterized. Individual GP molecular species in different body parts of scallops were also partially quantified. Most of the dominant GP species contained PUFA, especially eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3). Considering the high level of PUFA enriched GP, edible parts and by-products (gonads and mantles) of scallops Patinopecten yessoensis and Chlamys farreri provide great potential as health-promoting food for human consumption.
研究领域:
海洋生物学
·
营养学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2019
·
IF 3.579
Kai Huang,Wenwen Yu,Sen Li,Xiao Guan,Jing Liu,Hongdong Song,Dandan Liu,Ruiqian Duan
Embryo-remaining oat rice (EROR), as a newly developed oat product, is popular in China for its good taste, but little is known about its healthy functions. In this study, the effects of EROR on lipid metabolism regulation were investigated in in vitro and in vivo models. The results showed that the oat ethanol extracts significantly alleviated lipid accumulation, total cholesterol and triglyceride levels in HepG2 cells. EROR supplementation dramatically improved the lipid profile in the serum and liver and downregulated the expression levels of HMGCR, SREBP-1C and FAS, which are related to lipid metabolic disorder in high-fat diet (HFD) fed rats. A HFD decreases the production of short-chain fatty acids (SCFAs) in the cecum, which are related to intestinal microbiota dysbiosis. The intake of EROR significantly increased the total SCFAs, acetate and propionate and promoted the abundance of SCFA-producing bacteria. Furthermore, the intake of EROR led to abundant increases in Bifidobacterium and Akkermansia and decreases of Rombutsia , Fusicatenibacter , Holdemanella and Turicibacter , which were negatively and positively correlated with the lipid metabolism-related indices. These results provide evidence that EROR is a good functional food candidate to ameliorate lipid metabolic disorder and hyperlipidemia.
研究领域:
营养学
·
微生物学
·
代谢学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2020
·
IF 3.209
Jina Yang,Danyang Liu,Wenbin Jin,Qisheng Zhong,Ting Zhou
The pseudotargeted lipidomics method integrates the advantages of untargeted andtargeted lipidomics methods as a novel emerging approach. In this study, a green andefficient pseudotargeted lipidomics method based on ultra-high performancesupercritical fluid chromatography-tandem mass spectrometry (UHPSFC-MS/MS) wasdeveloped. The tandem mass spectra of the analytes were obtained by using UHPSFCwith quadrupole-time of flight MS (Q-TOF MS) in MS E mode and the multiplereaction monitoring (MRM) transitions of the lipidome were defined. Then, thecandidate MRM transitions were verified by UHPSFC with triple quadrupole massspectrometry (QqQ MS) in the scheduled MRM mode. In total, 758 potential lipidscorresponding to 509 and 249 MRM transitions were detected within 8 min in positiveand negative modes, respectively. The established pseudotargeted lipidomics methodwas validated to have excellent analytical characteristics. Compared with thepseudotargeted method based on ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), the UHPSFC-MS/MS-basedpseudotargeted method not only reduced the analytical time by half but also improvedthe sensitivity and resolution for most analytes, especially had better separation forlipid isomers. Besides, the UHPSFC-MS/MS-based pseudotargeted method showedhigher sensitivity and better repeatability for most analytes than the UHPSFC-MS/MS-based untargeted method. The established method was finally applied to investigatingthe lipid profiles of the plasma from the depressed rats and 33 differential variableswere screened, which related to three metabolic pathways. The results indicated thatthe UHPSFC-MS/MS-based pseudotargeted method is reliable and efficient and couldbe used in the lipidomics studies.
研究领域:
分析化学
·
脂质组学
·
药学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）