JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2021
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IF 4.556
Dong Zhang,Luyao Zhao,Wenjuan Wang,Qian Wang,Jianlei Liu,Yuanyuan Wang,Hui Liu,Bo Shang,Xiaoliang Duan,Hui Sun
In the current study, the changes of non-starch and starch lipids in two rice varieties during storage were investigated based on untargeted lipidomic approach. A total of 395 non-starch lipids and 13 starch lipids were identified in two rice varieties. 171 lipids in Nanjing and 6 lipids in Jianzhen were significantly different in stored rice compared to those in the fresh ones. Bioinformatics analysis on these significantly different lipids revealed that the two rice varieties possessed different lipids metabolic pathways during storage. Our study provides insights into the mechanism of the changes in non-starch and starch lipids in rice during storage.
研究领域:
植物学
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食品科学
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代谢组学
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产品关联
DOI
高影响因子
Metabolites
·
2022
·Vol. 12
·期 10
·977
·
IF 5.581
Xiaohui Feng,Jing Li,Longchao Zhang,Zhenghua Rao,Shengnan Feng,Yujiao Wang,Hai Liu,Qingshi Meng
Frozen storage is an important strategy to maintain meat quality for long-term storage and transportation. Lipid oxidation is one of the predominant causes of the deterioration of meat quality during frozen storage. Untargeted lipidomic and targeted metabolomics were employed to comprehensively evaluate the effect of frozen duration on pork lipid profiles and lipid oxidative products including free fatty acids and fatty aldehydes. A total of 688 lipids, 40 fatty acids and 14 aldehydes were successfully screened in a pork sample. We found that ether-linked glycerophospholipids, the predominant type of lipids, gradually decreased during frozen storage. Of these ether-linked glycerophospholipids, ether-linked phosphatidylethanolamine and phosphatidylcholine containing more than one unsaturated bond were greatly influenced by frozen storage, resulting in an increase in free polyunsaturated fatty acids and fatty aldehydes. Among these lipid oxidative products, decanal, cis-11,14-eicosenoic acid and cis-5,8,11,14,17-dicosapentaenoic acid can be considered as potential indicators to calculate the freezing time of unknown frozen pork samples. Moreover, over the three-month frozen storage, the first month was a rapid oxidation stage while the other two months were a slow oxidation stage.Keywords:lipidome;metabolome;lipid oxidation;pork;freezing process
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 10
·2095-2107
·
IF 7
Yu Wei,Ting He,Qunjie Bi,Huan Yang,Xueyi Hu,Rongrong Jin,Hong Liang,Yongqun Zhu,Rongsheng Tong,Yu Nie
The success of mRNA vaccines for COVID-19 prevention raised global awareness of the importance of nucleic acid drugs. The approved systems for nucleic acid delivery were mainly formulations of different lipids, yielding lipid nanoparticles (LNPs) with complex internal structures. Due to the multiple components, the relationship between the structure of each component and the overall biological activity of LNPs is hard to study. However, ionizable lipids have been extensively explored. In contrast to former studies on the optimization of hydrophilic parts in single-component self-assemblies, we report in this study on structural alterations of the hydrophobic segment. We synthesize a library of amphiphilic cationic lipids by varying the lengths (C = 8–18), numbers (N = 2, 4), and unsaturation degrees (Ω = 0, 1) of hydrophobic tails. Notably, all self-assemblies with nucleic acid have significant differences in particle size, stability in serum, membrane fusion, and fluidity. Moreover, the novel mRNA/pDNA formulations are characterized by overall low cytotoxicity, efficient compaction, protection, and release of nucleic acids. We find that the length of hydrophobic tails dominates the formation and stability of the assembly. And at a certain length, the unsaturated hydrophobic tails enhance the membrane fusion and fluidity of assemblies and thus significantly affect the transgene expression, followed by the number of hydrophobic tails.
研究领域:
药学
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分子生物学
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纳米技术
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药物递送系统
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病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-18
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IF 12.6
Wang Yingqian,Ma Zeqiang,Jiang Lehua,Bojan Nataraj,Sha Yiwen,Huang Boyu,Ming Lianxi,Shen Junnan,Pang Weijun
Background Skeletal muscle lipid deposition is a key manifestation of obesity, often accompanied by decreased exercise capacity and muscle atrophy. Skeletal muscle as the largest organ in the body, makes it challenges for designing targeted drug delivery systems. Lipid nanoparticles (LNPs) are widely used as a safe and efficient delivery carrier, there is limited research on LNPs that specifically target skeletal muscle. Results A LNP designed with five specific receptor complements on its surface, which specifically targets skeletal muscle in vivo in mice, without off-target effects on other tissues and organs. MiR-130a, a regulator of PPARG, which is a key factor in skeletal muscle lipid deposition, was encapsulated with LNP (LNP@miR-130a). In high-fat diet (HFD) mice, LNP@miR-130a effectively reduced skeletal muscle lipid deposition, increased exercise activity and enhanced muscle mass. Interestingly, the myokines in skeletal muscle have also changed which may leading to reduce the adipose tissue weight and liver lipid deposition in HFD mice. Conclusions These results indicated LNP@miR-130a is a promising inhibitor of skeletal muscle lipid deposition and may help alleviate obesity. This study provides new insights for obesity treatment and lays foundation for the development of targeted skeletal muscle therapeutics. Graphical
研究领域:
生物医学
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分子生物学
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药剂学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
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IF 8.3
Gege Zeng,Zepeng He,Haihong Yang,Zhan Gao,Xueer Ge,Lixin Liu,Zhijia Liu,Yongming Chen
Much of current clinical interest has focused on mRNA therapeutics for the treatment of lung-associated diseases, such as infections, genetic disorders, and cancers. However, the safe and efficient delivery of mRNA therapeutics to the lungs, especially to different pulmonary cell types, is still a formidable challenge. In this paper, we proposed a cationic lipid pair (CLP) strategy, which utilized the liver-targeted ionizable lipid and its derived quaternary ammonium lipid as the CLP to improve liver-to-lung tropism of four-component lipid nanoparticles (LNPs) for in vivo mRNA delivery. Interestingly, the structure–activity investigation identified that using liver-targeted ionizable lipids with higher mRNA delivery performance and their derived lipid counterparts is the optimal CLP design for improving lung-targeted mRNA delivery. The CLP strategy was also verified to be universal and suitable for clinically available ionizable lipids such as SM-102 and ALC-0315 to develop lung-targeted LNP delivery systems. Moreover, we demonstrated that CLP-based LNPs were safe and exhibited potent mRNA transfection in pulmonary endothelial and epithelial cells. As a result, we provided a powerful CLP strategy for shifting the mRNA delivery preference of LNPs from the liver to the lungs, exhibiting great potential for broadening the application scenario of mRNA-based therapy.
研究领域:
药学
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纳米技术
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分子生物学
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治疗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
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2016
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IF 3.687
Yinghuan Li,Lei Gao,Xi Tan,Feiyang Li,Ming Zhao,Shiqi Peng
The clathrin-mediated endocytosis is likely a major mechanism of liposomes' internalization. A kinetic approach was used to assess the internalization mechanism of doxorubicin (Dox) loaded cationic liposomes and to establish physiology-based cell membrane traffic mathematic models. Lipid rafts-mediated endocytosis, including dynamin-dependent or -independent endocytosis of noncaveolar structure, was a dominant process. The mathematic models divided Dox loaded liposomes binding lipid rafts ( B ) into saturable binding ( SB ) and nonsaturable binding ( NSB ) followed by energy-driven endocytosis. The intracellular trafficking demonstrated early endosome-late endosome-lysosome or early/late endosome-cytoplasm-nucleus pathways. The three properties of liposome structures, i.e. , cationic lipid, fusogenic lipid, and pegylation, were investigated to compare their contributions to cell membrane and intracellular traffic. The results revealed great contribution of cationic lipid DOTAP and fusogenic lipid DOPE to cell membrane binding and internalization. The valid Dox in the nuclei of HepG2 and A375 cells treated with cationic liposomes containing 40 mol% of DOPE were 1.2-fold and 1.5-fold higher than that in the nuclei of HepG2 and A375 cells treated with liposomes containing 20 mol% of DOPE, respectively, suggesting the dependence of cell type. This tendency was proportional to the increase of cell-associated total liposomal Dox. The mathematic models would be useful to predict intracellular trafficking of liposomal Dox.
研究领域:
药理学
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细胞生物学
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肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
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2025
·
IF 3.2
Liu Xiaolong,Peng Wu,Ouyang Hui,Wang Ping,Yu Xiongwei,Xie Tingting,Huo Jiaying,Li Shugang
The oxidative stability of walnut kernels during storage has a significant impact on their industrial development. However, the effect of storage conditions on the oxidation of walnut kernel lipids and its mechanism have not been systematically elucidated. Therefore, in this study, the lipid oxidation process in walnut kernels under different storage conditions (room temperature-air, room temperature-vacuum, low temperature-air, and low temperature-vacuum) was systematically studied from the perspective of phenomena, substances, and mechanisms using HPLC–MS and GC–MS methods. Experimental results found the walnut kernels stored at room temperature in air showed the highest PV (7.78 mmol/kg), conjugated diene (1.29), and hexanal (848.65 ng/g) after a 9-month storage. A total of 597 lipid substances were detected in walnut kernels under the four storage conditions, including 5 classes and 26 subclasses, of which 115 were significantly different. Differential lipid metabolite analyses indicated that storage conditions mainly affected the glycerophospholipid and glycerolipid metabolic pathways of walnut kernels, followed by linoleic acid metabolism. It revealed that temperature, rather than air conditions, played a dominant role in the lipid oxidation of walnut kernels during storage. This experiment could provide a comprehensive understanding of the lipid oxidation of stored walnut kernels and technical support for quality control. Graphical abstract
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED MATERIALS
·
2025
·
IF 26.8
Hao Zheng,Jiangbangrui Chu,Shikang Li,Xun Tang,Shicheng Bi,Kerong Chen,Anwei Zhou,Youwen Yang,Xinghai Ning,Yurui Xu
Elderly patients with hyperlipidemia often exhibit resistance to conventional hypolipidemic treatments, underscoring the need for more effective strategies to address lipid imbalances in this high-risk group. This study introduces LipClean, an aerogel-based apheresis device specifically designed to remove harmful plasma lipids. LipClean is constructed using hydrophilic cellulose fibers, which serve as a supramolecular platform for synthesizing hydrophobic conjugated polymers through a Sonogashira-Hagihara reaction. These conjugated polymers are then cross-linked with the cellulose fibers via phosphorylation, generating an aerogel monolith with an interpenetrating network of hydrophilic fibers and hydrophobic polymers. Unlike bilayer aerogels that separate hydrophilic and hydrophobic layers, LipClean's interpenetrating structure is precisely engineered through polymer design and gradient cross-linking. This optimization enhances both bodily fluid flow and lipid adsorption while minimizing the removal of essential plasma components and ensuring unobstructed cell passage. In preclinical testing, LipClean significantly reduced triglyceride and cholesterol levels in an elderly rat model of hyperlipidemia and normalized lipid levels in blood samples from hypertensive patients. Importantly, purified blood maintained normal levels of blood cells and physiological and biochemical indicators after apheresis, highlighting LipClean's potential for managing hyperlipidemia-related disorders. This study, therefore, underscores the importance of interdisciplinary collaboration in driving medical device innovation.
研究领域:
医学工程
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生物材料
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心血管病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 62
·35600-35610
·
IF 2.936
Hong-lian Zhang,Ai-hua Zhang,Xiao-hang Zhou,Hui Sun,Xiang-qian Wang,Liu Liang,Xi-jun Wang
Altered lipid metabolism is an emerging hallmark of cancers. Mirabilite has a therapeutic effect on colorectal cancer (CRC); however, its metabolic mechanism remains unclear. This study aims to explore the potential therapeutic targets of mirabilite protection against colorectal cancer in APCmin/+ mice model. Oral administration of mirabilite was started from the ninth month, while the same dosage of distilled water was given to both the control group and the model group. Based on lipidomics, we collected serum samples of all mice at the 20th week and used a non-targeted method to identify the lipid biomarkers of CRC. Compared with C57BL/6J mice, the metabolic profile of CRC model mice was significantly disturbed, and we identified that 25 lipid-related biomarkers, including linoleic acid, 2-hydroxybutyric acid, 6-deoxocastasterone, hypoxanthine, PC(16:1), PC(18:4), and retinyl acetate, were associated with CRC. According to the abovementioned results, there were six lipid molecules with significant differences that can be used as new targets for handling of CRC through six metabolic pathways, namely, linoleic acid metabolism, retinol metabolism, propanoate metabolism, arachidonic acid metabolism, biosynthesis of unsaturated fatty acids and purine metabolism. Compared with the model group, the metabolic profiles of these disorders tend to recover after treatment. These results indicated that the lipid molecules associated with CRC were regulated by mirabilite. In addition, we identified seven key lipid molecules, of which four had statistical significance. After administration of mirabilite, all disordered metabolic pathways showed different degrees of regulation. In conclusion, high-throughput lipidomics approach revealed mirabilite regulating the altered lipid metabolism as anticancer therapeutics.
研究领域:
肿瘤学
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代谢组学
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药理学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Zhiqing Liu,Mantong Zhao,Xinwen Wang,Chuan Li,Zhongyuan Liu,Xuanri Shen,Dayong Zhou
The present study sought to analyze the lipid profiles of oyster Crassostrea gigas from Yellow Sea (YS), East China Sea, and South China Sea (SCS) through the untargeted lipidomics strategy based on UPLC-Q-Exactive Orbitrap mass spectrometry and multivariate statistics. The results elucidated that geographical differences had profound effects on the lipid content, composition, and lipid molecular profiles. Notably, oysters from the YS group contained the highest lipid content, including triacylglycerol, diacylglycerols, and the majority of phospholipid molecule species, while oysters from the ECS group contained most of the phosphatidylcholine species and the SCS group contained most of the sphingolipid species. Totally, 1155 lipid molecular species belonging to 21 subclasses were identified; of them, 45 lipid molecular species could serve as differential marker for lipid of oysters from different sea areas. Overall, lipidomics could be a potential approach for discrimination of lipid characters between marine shellfishes for geographical origin traceability.
研究领域:
海洋生物学
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分析化学
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食品科学
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Shuang Cui,Ying Wang,Zheng Zhou,Yu-Ying Zhang,Xu-Hui Huang,Da-Yong Zhou,Lei Qin
Lipid composition is crucial for assessing the nutrition and flavor of animal-derived raw materials, a key concern for consumers. Based on high-resolution mass spectrometry and chemical pattern recognition, the intermuscular lipids fingerprint of seven types of livestock, poultry, and fish muscle were analyzed. In total, 704 lipids were detected, containing fourteen lipid molecules, with significant content differences among samples, notably triglycerides, phosphatidylcholines, phosphatidylethanolamines, diglycerides, and fatty acids. Livestock and poultry muscle had a low content of lipids with an unsaturation degree above 4 (around 10% and 25%). These unsaturated lipid molecules in fish muscle ranged from approximately 60%–75%. Rainbow trout had higher contents of triglycerides and phosphatidylcholines with chain lengths exceeding 13. Mutton had more of these lipids within the 13–18 chain length, exceeding 0.3 g/(g·protein), which may be crucial for their unique texture and flavor. The results will help the deep processing and nutritional assessment of muscle foods.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2019
·
IF 3.532
Zheng Zhao,Weihe Yao,Ning Wang,Chenyu Liu,Hengjun Zhou,Hailiang Chen,Weihong Qiao
Cationic lipids can easily assemble into spherical liposomes in aqueous phase which showed unique superiority in drug and gene delivery. However, the toxicity of cationic lipids is still an obstacle to application. To develop low toxicity cationic lipids, we designed two cationic lipids contained different number of hydroxyl groups . Biocompatible mono-hydroxyl and multi-hydroxyl galactose head group was respectively modified to a biodegradable quaternary amine lipid, and two novel hydroxyl cationic lipids were synthesized and characterized by MS, 1 H NMR and 13 C NMR. Two lipids showed good surface activity and both of them can assemble to about 80 nm stable small unilamellar vesicles (SUVs) with cholesterol in aqueous phase. Both of lipids showed relatively lower toxicity than the well-known cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). In vitro 24 h IC 50 of two assemblies were more than 50 μg/mL, which were about 10 μg/mL higher than the IC 50 of DOTAP. Multi-hydroxyl galactose lipids group showed much lower toxicity than mono-hydroxyl lipids group. Moreover, Both of the assemblies with lower hemolysis were nearly non-hemolytic risk under the concentration of 30 μg/mL. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) showed that the average sizes of both doxorubicin (DOX) loaded liposomes were about 110 nm. The DOX entrapment efficiencies of galactose liposome and mono-hydroxyl liposome were 58% and 91%, respectively. Both of the DOX loaded liposomes were stable after one month placed at room temperature. Two DOX loaded liposomes showed better anti-cancer effect than free DOX above 5 μg/mL, and they can be internalized into cells and produce more release of DOX inside MCF-7 cells and HepG2 cells at pH 5.0. These results suggested that synthesized lipids are suitable as potential low toxicity cationic drug delivery systems .
研究领域:
药学
·
生物化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）