SURFACE & COATINGS TECHNOLOGY
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2023
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IF 5.4
Cong Wu,Chun Zhang,Xianyi Yan,Junhua Cheng,Lei Chen,Yufei Tang,Kang Zhao
Titanium implants have the risk of loosening or even failure, especially the force generated by chewing is not conducive to the use of conventional titanium bioactive coatings in the oral environment. In this work, a BCZT/TiO 2 bio-piezoelectric coating was prepared on titanium surface. The force generated by chewing is cleverly converted into the piezoelectric charge on the coating surface , and the formation of the combination of implant and alveolar bone is accelerated by its promoting effect on bone repair. The formation mechanism of the coating was studied. The BCZT/TiO 2 bio-piezoelectric coating showed good piezoelectric properties . The calcium deposition results of BCZT/TiO 2 coating with static , physiological loading and low-frequency ultrasonic conditions were compared. Interestingly, the effect of polarized BCZT/TiO 2 coating applied with low-intensity pulsed ultrasound on promoting calcium deposition was similar to that applied with physiological loading, suggesting that low-intensity pulsed ultrasound has the potential to stimulate piezoelectric effects. In addition, the BCZT/TiO 2 coating was non-toxic and has good biocompatibility and bioactivity. The polarized BCZT/TiO 2 coating showed the best proliferation results, and the osteoblast adhesion morphology on the coating surface was extended. The results provide data support for the application of titanium bio-piezoelectric coating on dental implants .
研究领域:
生物材料学
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口腔医学
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生物力学
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产品关联
DOI
高影响因子
Bioactive Materials
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2024
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IF 18
Yao Zhao,Yunfan Cai,Wenkai Wang,Yongkang Bai,Mingyi Liu,Yan Wang,Wen Niu,Zhixiao Luo,Lingyun Xia,Juanfang Zhu,Fei Zhao,Franklin R. Tay,Lina Niu
The development of scaffolds for repairing critical-sized bone defects heavily relies on establishing a neuro-vascularized network for proper penetration of nerves and blood vessels. Despite significant advancements in using artificial bone-like scaffolds infused with various agents, challenges remain. Natural bone tissue consists of a porous bone matrix surrounded by a neuro-vascularized periosteum, with unique piezoelectric properties essential for bone growth. Drawing inspiration from this assembly, we developed a periosteum-bone-mimicking bilayer scaffold with piezoelectric properties for regeneration of critical-sized bone defects. The periosteum-like layer of this scaffold features a double network hydrogel composed of chelated alginate, gelatin methacrylate, and sintered whitlockite nanoparticles, emulating the viscoelastic and piezoelectric properties of the natural periosteum. The bone-like layer is composed of a porous structure of chitosan and bioactive hydroxyapatite created through a biomineralization process. Unlike conventional bone-like scaffolds, this bioinspired bilayer scaffold significantly enhances osteogenesis, angiogenesis, and neurogenesis combined with low-intensity pulsed ultrasound-assisted piezoelectric stimulation. Such a scheme enhances neuro-vascularized bone regeneration in vivo . The results suggest that the bilayer scaffold could serve as an effective self-powered electrical stimulator to expedite bone regeneration under dynamic physical stimulation.
研究领域:
生物医学工程
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组织工程
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再生医学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2013
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IF 3.479
Yun Lu,Qingfeng Sun,Xilin She,Yanzhi Xia,Yixing Liu,Jian Li,Dongjiang Yang
α-Chitin nanofibers were fabricated with dried shrimp shells via a simple high-intensity ultrasonic treatment under neutral conditions (60 KHz, 300 W, pH = 7). The diameter of the obtained chitin nanofibers could be controlled within 20–200 nm by simply adjusting the ultrasonication time. The pulsed ultrasound disassembled natural chitin into high-aspect-ratio nanofibers with a uniform width (19.4 nm after 30 min sonication). The EDS, FTIR, and XRD characterisation results verified that α-chitin crystalline structure and molecular structure were maintained after the chemical purification and ultrasonic treatments. Interestingly, ultrasonication can slightly increase the degree of crystallinity of chitin (from 60.1 to 65.8). Furthermore, highly transparent chitin films (the transmittance was 90.2% at a 600 nm) and flexible ultralight chitin foams were prepared from chitin nanofiber hydrogels.
研究领域:
材料科学
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生物学
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产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
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2025
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IF 6.5
Zhiying Zhang,Lijun Liu,Huan Wang,Wangni Xie,Wenhao Zhai,Linlin Wen,Boya Zhang,Kexuan Liu,Xue Zhang,Shuchen Liu,Lei Huang,Daowei Li,Yanmin Zhou
Objective Large full-thickness skin wounds pose significant challenges, particularly in achieving scar-free healing and the regeneration of skin appendages. This study introduces a portable approach for promoting scarless healing and skin appendage regeneration by utilizing low-intensity pulsed ultrasound (LIPUS) activated piezoelectric electrospun membranes to modulate the local electrical environment.Methods Dopamine-modified polyvinylidene fluoride (DA/PVDF) nanomembranes were fabricated via electrospinning, followed by piezoelectric characterization under varying LIPUS stimulation. Cell adhesion was examined using SEM and laser confocal microscopy to assess surface interactions. Cell proliferation and migration were further analyzed using the CCK-8 assay and Transwell migration assay, respectively. Finally, the effects of DA/PVDF membranes on full-thickness skin defect healing were tested in a mouse model. The healing process was documented with photographs, and functional skin regeneration was evaluated through histological analysis.Results The DA/PVDF nanomembranes had an average diameter of 732 ± 232 nm and generated a voltage of 450 mV under LIPUS stimulation, a 1.28-fold increase compared to PVDF membranes alone. In vitro, LIPUS-activated membranes enhanced cell adhesion and proliferation, resulting in a 1.14-fold increase in cell growth over three days. The transwell migration assays showed 244.67 ± 7.85 migrated cells. In vivo, the DA/PVDF+LIPUS group exhibited significantly higher wound healing rates, with improved epidermal regeneration, collagen fiber deposition and remodeling, and enhanced blood vessel and skin appendage formation.Conclusion DA modification enhances the piezoelectric properties of PVDF membranes, boosting cell adhesion and promoting dermal and vascular regeneration. LIPUS-generated mechanical waves effectively stimulate membrane deformation, producing a localized electrical microenvironment that mimics the natural bioelectric field of skin and accelerates functional wound healing.
研究领域:
生物医学工程
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组织工程
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再生医学
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产品关联
DOI
PubMed
高影响因子
Materials Today Bio
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2025
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IF 10.2
Meng Li,Xiantong Hu,Xiaofei Liu,Lingzhou Zhao,Wanmin Zhao,Ying Li,Xiaomei Bie,Hua Jiao,Yantao Zhao,Chufan Ma
Bone defects resulting from trauma, tumor resection, non-union of fractures, and infections present enormous challenges in treatment. Although three-dimensional (3D) bioprinting plays an important role in repairing bone tissues, the lack of mechanical properties and osteoinductive ability of the bioinks remains a barrier for the application of the technology. In this study, we used advanced 3D bioprinting technology to create a novel piezoelectric hydrogel scaffold (Gel/PBT@BMSCs) which consisted of bone marrow-derived mesenchymal stem cells (BMSCs), gelatin methacryloyl (GelMA), and polyethylene glycol (PEG)-modified barium titanate (BT) nanoparticles. The piezoelectric hydrogel scaffold provided a stable 3D microenvironment for cell growth and adhesion, enhancing cell viability and osteogenic activity when subjected to low-intensity pulsed ultrasound (LIPUS) stimulation. Furthermore, in vivo experiments demonstrated that the innovative hydrogel scaffold significantly accelerated the healing process of bone defects and exhibited impressive bone regeneration capabilities. These findings highlight the potential of piezoelectric hydrogel for further research and application in the field of bone tissue engineering, and offer new approaches for the treatment of bone defects.
研究领域:
组织工程
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再生医学
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生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioengineering & Translational Medicine
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2024
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IF 6.1
Juan Deng,Juan Qin,Guolin Song,Chenghai Li,Wentao Tang,Yilin Tang,Xinfang Xiao,Liu Wu,Sicheng He,Yiqing Zhou,Junfen Li,Yan Wang
The potential of low-intensity pulsed ultrasound (LIPUS) in regulating ovarian function has been demonstrated; however, there is a lack of scientific evidence regarding the long-term efficacy of LIPUS in treating ovarian injury and understanding its regulatory mechanisms. In this study, 4-vinylcyclohexene diepoxide (VCD) was used to induce ovarian injury in rats, and LIPUS was applied to target the damaged ovarian tissues. The research aimed to investigate the long-term protective effect of LIPUS against ovum toxicity induced by VCD and elucidate the associated molecular mechanisms. During the experiment, HE staining was employed for observing the morphology and structure of the ovary, while protein sequencing was utilized for identifying and confirming the molecular mechanism through which LIPUS restores the damaged ovarian structure. The long-term effectiveness of LIPUS in protecting against ovarian injury was evaluated through ELISA, estrous cycle monitoring, fertility testing, and behavioral analysis. The results indicated that LIPUS effectively restored the structure of damaged ovaries. Both in vivo and in vitro studies revealed that this protective effect may be attributed to LIPUS inhibiting apoptosis of ovarian granulosa cells (GCs) by regulating Daxx-mediated ASK1/JNK signaling pathway. Subsequent functional tests demonstrated significant improvements in sex hormone secretion and regulation of estrous cycle within 6 cycles following LIPUS treatment. Additionally, there was a notable increase in offspring numbers after mating. Behavioral analysis revealed that LIPUS effectively alleviated menopausal symptoms resulting from ovarian injury including mood fluctuations, cognitive behavior changes, and reduced muscle excitability levels. These findings suggest that beneficial effects of LIPUS may help reduce VCD-induced ovarian damage with long-term efficacy.
研究领域:
生殖医学
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分子生物学
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内分泌学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
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IF 4.952
Xizhe Fu,Di Wang,Tarun Belwal,Jing Xie,Yanqun Xu,Li Li,Ligen Zou,Lixia Zhang,Zisheng Luo
A deep understanding upon the relation between anthocyanin extraction performance and physicochemical properties of natural deep eutectic solvents (NADESs) was obtained, and a high-efficiency NADES (Choline chloride-Oxalic acid (ChOa)) was selected and coupled with pulse-ultrasonication for anthocyanin extraction from blueberry pomace . Response surface methodology was adopted to analyzed the significant factors’ influence, interactive effects on extraction yield and optimize the extraction conditions. The theoretical optimal conditions with highest yield of total anthocyanins content (TAC) were 3.2 min of ultrasonic time, 325 W of ultrasonic power, 349.15 K of temperature, 60 mL/g of solvent to solid ratio, under which the experimental TAC value (24.27 ± 0.05 mg C3GE/g DW) was highly correlated with theoretical result (24.28 mg C3GE/g DW). Multi-stability protective effect of ChOa on anthocyanin over acidified ethanol was revealed by stability assays in high temperature, varying pH and light exposure conditions. FT-IR and DSC analysis further characterized the influence of ChOa over acidic ethanol on typical anthocyanins functional groups and thermal behavior of anthocyanin extracts. UPLC-Triple-TOF/MS analysis identified 10 anthocyanins that mainly consisted of delphinidin , petunidin , and malvidin compounds. Overall, this study provided a green, high-efficiency and multi-stability protective strategy for the recovery of anthocyanins from plant source by-products.
研究领域:
食品科学
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化学工程
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分析化学
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植物科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）