INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Shima Ostovar,Mehrab Pourmadadi,Amin Shamsabadipour,Parsa Mashayekh
Curcumin (CUR) is among the most appropriate and natural-based anticancer drugs that can be applied effectively treat different classes of cancers. However, CUR suffers from a low half-life and stability in the body, which has restricted the efficacy of its delivery applications. This study is dedicated to introducing the pH-sensitive nanocomposite of chitosan (CS)/gelatin (GE)/carbon quantum dots (CQDs) as an applicable nanocarrier for enhancing CUR half-life and its delivery restrictions. The CS/GE hydrogel was synthesized by the physical crosslinking method, which improves the biocompatibility of this hydrogel. Moreover, the water-in-oil-in-water (W/O/W) double emulsion approach is involved in fabricating the drug-loaded CS/GE/CQDs@CUR nanocomposite. Afterward, drug encapsulation (EE) and loading efficiencies (LE) have been determined. Furthermore, FTIR and XRD assessments were performed to confirm the CUR incorporation into the prepared nanocarrier and crystalline features of the nanoparticles. Then, by employing Zeta potential and dynamic light scattering (DLS) analysis, the size distribution and stability of the drug-loaded nanocomposites have been assessed, which indicated monodisperse and stable nanoparticles. Furthermore, field emission scanning electron microscopy (FE-SEM) was utilized that confirmed the homogeneous distribution of the nanoparticles with smooth and quite spherical structures. In vitro drug release pattern was studied and the kinetic analysis was performed using a curve fitting technique to determine the governing release mechanism at both acidic pH and physiological conditions. The obtained outcomes from release data revealed a controlled release behavior with a 22-hour half-life, while the EE% and EL% were acquired at 46.75 % and 87.5 %, respectively. In addition, the MTT assay has been carried out on U-87 MG cell lines to evaluate the cytotoxicity of the nanocomposite. The findings showed that the fabricated nanocomposite of CS/GE/CQDs can be assumed as a biocompatible CUR nanocarrier, while the drug-loaded nanocomposite of CS/GE/CQDs@CUR showed enhanced cytotoxicity compared to the pure CUR. Based on the obtained results, this study suggests the CS/GE/CQDs nanocomposite as a biocompatible and potential nanocarrier for ameliorating CUR delivery restrictions to treat brain cancers.
研究领域:
生物医学
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材料科学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
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2025
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IF 5.1
Mehrab Pourmadadi,Amir Reza Hafezi,Roya Yaghoobi,Aynaz Rahmaninodeh,Negin Hosseini,Afshin Alibabaie,Narges Ajalli,Elaheh Babajani Afrouzi,Mahdi Shirazi,Azam Ghadami
This research examines the formulation and characterization of a pH-sensitive nanocarrier for the targeted and controlled release of curcumin (Cur) in the treatment of brain cancer. The nanocarrier consists of gelatin (G), agarose (Aga), and magnesium-doped carbon quantum dots (Mg-CQD), produced by a water-in-oil-in-water (w/o/w) double emulsification method. These materials demonstrate pH responsiveness, biocompatibility, and biodegradability, crucial for addressing Cur's limitations, including inadequate solubility and fast metabolism. In vitro cytotoxicity assays on U-87 MG glioma cells showed a 49 % reduction in cell viability, whereas minimal toxicity was observed in L929 fibroblast cells, indicating selective anticancer potential. The nanocarrier exhibited a regulated and extended release of Cur under acidic conditions, achieving encapsulation and loading efficiencies of 45.75 % and 85.5 %, respectively, hence enhancing its bioavailability and therapeutic efficacy. Characterization confirmed uniform nanoparticle distribution (average size: 193.5 nm; zeta potential: −42 mV) and successful drug encapsulation. FTIR, XRD and FE-SEM tests validated the effective drug encapsulation, uniform distribution of nanoparticles, and stability. The findings underscore the nanocarrier's potential for targeted cancer therapy, providing both therapeutic efficacy and safety, with intriguing applications in nanomedicine.
研究领域:
生物医学工程
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药剂学
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肿瘤学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
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2021
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IF 6.057
Jian Zhang,Chengan Guo,Xinming Huo,Xiaoxiao Ma,Xin Li,Zeper Abliz,Yao Chu,Xiaohao Wang,Fei Tang
In recent years, the development of unsaturated lipid isomeric imaging based on the Paternò–Büchi (PB) reaction has improved significantly. However, research on this imaging method in ambient conditions needs to expand. In this paper, a method of PB reaction in the solid phase in ambient conditions is developed, which is combined with air-flow-assisted desorption electrospray ionisation mass spectrometry (AFADESI-MS) to achieve in situ imaging of lipids at an isomeric level. Experiments showed that the efficiency of the PB reaction was much higher when spraying the reagent solution than when sprinkling the reactant powder directly, and it was not lower than that in the liquid phase. This method can simplify the reaction conditions in the imaging process and can be applied to tissue section samples with only 10 min of pre-processing. The study successfully demonstrated the spatial distribution of unsaturated lipid isomers, and the isomeric ratio corresponded to the lesion areas in mouse brain cancer tissues. Due to its simple operation and performance in ambient conditions, this method may be useful for future studies on lipid isomers in tissues.
研究领域:
分析化学
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生物医学工程
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质谱学
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脂质组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EMBO Molecular Medicine
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2024
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IF 9
CaoXiaona,LiJie,RenJinliang,PengJiajin,ZhongRuyue,HeJiahao,XuTing,YuZhenhua,JinHuawei,HaoSiqi,LiuRuiwei,XuBingzhe
Current brain tumor treatments are limited by the skull and BBB, leading to poor prognosis and short survival for glioma patients. We introduce a novel minimally-invasive brain tumor suppression (MIBTS) device combining personalized intracranial electric field therapy with in-situ chemotherapeutic coating. The core of our MIBTS technique is a wireless-ultrasound-powered, chip-sized, lightweight device with all functional circuits encapsulated in a small but efficient “Swiss-roll” structure, guaranteeing enhanced energy conversion while requiring tiny implantation windows ( ~ 3 × 5 mm), which favors broad consumers acceptance and easy-to-use of the device. Compared with existing technologies, competitive advantages in terms of tumor suppressive efficacy and therapeutic resolution were noticed, with maximum ~80% higher suppression effect than first-line chemotherapy and 50–70% higher than the most advanced tumor treating field technology. In addition, patient-personalized therapy strategies could be tuned from the MIBTS without increasing size or adding circuits on the integrated chip, ensuring the optimal therapeutic effect and avoid tumor resistance. These groundbreaking achievements of MIBTS offer new hope for controlling tumor recurrence and extending patient survival.
研究领域:
神经科学
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肿瘤学
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生物医学工程
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医疗器械
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）