RSC Advances
·
2017
·Vol. 7
·期 42
·26211-26219
·
IF 3.108
Dandan Li,Lunan Guo,Na Yang,Yao Zhang,Zhengyu Jin,Xueming Xu
The properties and functionalities of chitosan are closely related to its degree of deacetylation (DD). This study aimed to determine the DD of chitosans, which were of identical molecular weight, by a proposed electro-analytical system based on inductive methodology. Statistically significant differences in electrical parameters, including terminal voltage (U′S) and λ (the ratio of primary voltage to U′S) were observed among chitosans with different DDs. Both U′S and λ were linearly correlated with the DD value, regardless of frequency, suggesting that these electrical parameters can be used to determine the DD of chitosan. More chitosan's coils contributed to higher measurement accuracy, but the change in measurement position showed no improvement. The highest linear coefficient of determination (0.9941) between DD and U′S was obtained by the experimental transformer with 100-turn chitosan's coils at a primary voltage of 10 V and a frequency of 400 Hz. This study explored the potential of an innovative electro-analytical system to determine the DD of chitosan.
研究领域:
生物化学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Ning Yan,Xiao-Fang Wan,Xin-Sheng Chai
This study reported a new headspace gas chromatography (HS-GC) for simultaneously determining the degree of deacetylation (DD) and the degree of substitution (DS) in carboxymethyl chitosan (CMCS), which were based on HS-GC measuring the amounts of CO2 released from both the bicarbonate decomposition suppressed by -NH2 and the reaction between the bicarbonate and the acidified carboxymethyl and amino groups in CMCS. The results showed that the present method has a good measurement precision (RSD < 2.55%) and accuracy (relative differences <5.90%). Compared with the current titration-based method, the present HS-GC techniques provide a more reliable testing in the quantification of amino and carboxymethyl contents in CMCS. Moreover, since the HS-GC can perform an automated sample reaction equilibration and measurement, it could be much more efficient than the existing methods in the batch sample analysis.
研究领域:
分析化学
·
生物材料学
✓ 已验证
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Song Zhang,Yang Shen,Peng Qiu,Mengjia Fang,Peichuang Li,Xiangning Du,Zhengying Deng,Wanhao Cai,Hui-Li Wang
The specific binding capacity between chitosan and heavy metal ions is critical in food safety, which is closely regulated by its degree of deacetylation (DD). However, the underlying mechanism governing the impact of DD remains unclear. Here we employ a combination of experiments and simulations to study the interaction between chitosan and Pb 2+ across multiscale at different DD. At the molecular level, Pb 2+ can chelate individual chitosan chains simultaneously via multiple interaction sites, which is enhanced with rising DD and leads to more ordered molecular packing. This molecular arrangement then develops to a nanoscale aggregation process, where the aggregation size, rate, and mechanical stability all show a positive correlation with DD. Such aggregation ultimately leads to enhanced macroscale structures, where the strain and tensile stress are significantly increased by 2 to 3-folds and further rises with increasing DD. These observed effects are governed by a unified mechanism: deacetylation introduces more amino groups that serve as stronger chelation sites than acetyl groups, thereby enhancing Pb 2+ binding across length scales. These findings provide foundation for the development and application of chitosan in the food industry, such as food preservation, anti-heavy-metal packaging, and food purification.
研究领域:
食品科学
·
高分子材料学
·
环境毒理学
·
食品安全
·
分子生物学
·
纳米科技
✓ 已验证
产品关联
DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2019
·
IF 4.511
Wenjun Liu,Yunfa Ma,Lihao Ai,Wenling Li,Wenyan Li,Hong Li,Changren Zhou,Binghong Luo
In order to study the relationship between the degree of deacetylation of chitin whiskers (CHWs) and their enzymatic degradation properties, in this paper, CHWs were first deacetylated to different degrees by alkali treatment, and the CHWs with the degrees of deacetylation of 17.84, 67.76, and 82.54% was obtained, respectively. Moreover, the partially deacetylated CHWs still maintained good crystallinity and nanoneedle-like morphology. Next, the in vitro degradation behavior of CHWs with different degrees of deacetylation was further studied under the single or synergistic action of lysozyme and lipase (37 °C, pH = 7.4). The results showed that the morphology change of CHWs was more obvious as the degree of deacetylation increased. The mass loss, the crystallinity index at the (110) crystal plane, and the concentration of reducing sugar of the CHWs also increased with the degree of deacetylation. Moreover, the synergistic effect of the two enzymes was more conducive to the degradation process of CHWs than single lysozyme or lipase. The difference in the rate of enzymatic degradation provides an idea for the regulation of the degradation rate of the CHWs.
研究领域:
材料科学
·
生物技术
·
纳米技术
·
酶学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yiwen Lu,Wen Geng,Lili Li,Fang Xie,Mingchang Zhang,Huatao Xie,Jie Cai
Multidrug-resistant (MDR) bacterial infections pose a severe threat to global public health and present significant challenges in the treatment of bacterial keratitis. The escalation of antimicrobial resistance (AMR) underscores the urgent need for alternative therapeutic strategies. In this study, we report the homogeneous synthesis of quaternized ultra-highly deacetylated chitosan (QUDCS) using a sequential acid-base combination approach. The optimized QUDCS-2 exhibits broad-spectrum antibacterial activity through a membrane-disruption mechanism driven by electrostatic, hydrogen bonding, and hydrophobic interactions, while maintaining low cytotoxicity and high selectivity. Compared to less deacetylated counterparts, QUDCS-2 demonstrates superior stability in enzyme-rich environments and effectively inhibits and eradicates mature biofilms of methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa . Furthermore, QUDCS-2 exhibits a remarkable ability to prevent the development of antimicrobial resistance. In a mouse keratitis model, QUDCS-2 shows excellent biocompatibility and significant antibacterial efficacy, providing strong support for its potential as a long-term, effective antimicrobial agent.
研究领域:
微生物学
·
材料科学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Yuntao Wang,Hui Zhang,Qing Liang,Xiuqin Guo,Zijian Niu,Si Qiu,Wei Xu,Rui Li
In this study, the effect of konjac glucomannan (KGM) with different degrees of deacetylation (DD) on the gel properties of transglutaminase induced soybean protein isolate (SPI) emulsion gel was investigated. It was found that the three kinds of KGM increased the breaking force and water holding capacity , enhanced the freeze-thaw stability of the SPI emulsion gel, while KGM with DD of 59% (DKGM1) improved these properties to the largest extent. This was because SPI-DKGM1 had the lowest oil water interfacial tension, resulting in the formation of the most uniform and the smallest oil droplets, which then led to the formation of much denser gel network. Moreover, the hydrophobic interaction and hydrogen bonding between DKGM1 and SPI were the strongest compared with that of other KGM. Besides, FTIR results showed that SPI-DKGM1 emulsion gel had higher β-sheet content, which was conductive to the formation of a stable three-dimensional network structure. However, the combination of SPI and KGM with high DD (DKGM2, DD = 92%) destroyed the gel structure to some extent and resulted in emulsion gel with weakened gel properties compared with that of DKGM1. Therefore, KGM with proper DD was conducive to the formation of more ordered and much denser gel network structure, which then resulted in SPI emulsion gel with the best gel properties.
研究领域:
食品科学
·
化学
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）