FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Rui Wang,Mei Li,Jinguang Liu,Fenfen Wang,Jing Wang,Zhongkai Zhou
The effect of dual modifications of pullulanase debranch and propionylation on rice starch property was investigated in this study. The occurrence of the propionylation in starch molecules was confirmed due to the formation of a new peak at 1750 cm −1 in FTIR spectrum and the characteristic peaks around 1.0 (methyl) and 2.2 (methylene) ppm in NMR compared with the native starch. Interestingly, because of the higher tendency of molecular retrogradation following the debranching, the current results revealed that there was no positive correlation between the amylose content (AC) and the debranching time. Debranching treatment increased the degree of substitute (DS) of the propionyl starch, and the AC was positively correlated with DS (R = 0.7753*), indicating that AC is an important element affecting propionyl reaction due to the exposure of more available hydroxyl groups during debranching. Meanwhile, debranching led to an increased crystallinity level and the crystallinity pattern changed from A-type to B-type, which is associated with the changes in their corresponding hydrolysis resistance. The NMR data also proved that the propionylation involves the replacements of the glucose in the starch structure (C-2, C-3 and C-6) with acyl groups of the desired complexes. Furthermore, the sequence of the reactions that occur in the process of propionylation and their mechanisms are proposed in the current study.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xinyu Wang,Qi Zhao,Ruijie Li,Wei Liang,Qian Lin,Li Niu,Wenqing Zhao,Wenhao Li
This study aimed to probe the influence of amylose in starch granules on starch modification. Part of the amylose from sorghum starch was removed through warm water leaching, and the samples were then microwaved. The effects of treatments on starch structure, physicochemical properties, and digestibility were researched. Compared to sorghum starch without partial amylose removal, warm water leaching of amylose resulted in deeper concavity of sorghum starch granules and lower crystallinity, short-range ordering, setback and breakdown values, which disrupted the structure and thus rendered the starch granules more susceptible to microwave radiation, amplifying the advantages of microwave-modified starch. In addition, the ordered arrangement of amylose and amylopectin structures within the microwave-treated sorghum starch with pre-removed amylose were more readily disrupted, allowing enzymes to pass through more readily, thus increasing the fast-digestible starch content and digestibility of the starch. In summary, partial amylose removal can be used as a pretreatment method for modifying and expanding starch utilization in food and non-food industries while providing a new idea for developing baked foods.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qianying Ma,Xiaolong Wang,Rudi Appels,Di Zhang,Xinyu Zhang,Liang Zou,Xinzhong Hu
The starch digestibility of flour is influenced by both physicochemical treatment and flour particle size, but the interactive effect of these two factors is still unclear. In this study, the effect of pullulanase debranching, combined with heat-moisture treatment (P-HMT), on starch digestibility of multi-grain flours (including oat, buckwheat and wheat) differing in particle size was investigated. The results showed that the larger-size flour always resulted in a higher resistant starch (RS) content either in natural or treated multi-grain flour (NMF or PHF). P-HMT doubled the RS content in NMFs and the large-size PHF yielded the highest RS content (78.43 %). In NMFs, the cell wall integrity and flour particle size were positively related to starch anti-digestibility. P-HMT caused the destruction of cell walls and starch granules, as well as the formation of rigid flour aggregates with B + V starch crystallite. The largest flour aggregates with the most ordered B + V starch were found in large-size PHF, which contributed to its highest RS yield, while the medium- and small-size PHFs with smaller aggregates were sensitive to P-HMT, resulting in the lower ordered starch but stronger interactions between starch and free lipid or monomeric proteins, eventually leading to their lower RS but higher SDS yield.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Chen-Chen Wang,Bao-Qiu Yang,Ran Feng,Han Tao,Bao-Cai Xu,Bao Zhang
In this study, biotin esterified debranched starch (Bio-DBS) nanoparticles with different molecular weights were prepared to improve the stability and antioxidant activity of resveratrol. The molecular weights of branched starch (DBS 3 , DBS 9 and DBS p ) determined by high-performance size-exclusion chromatography (HPSEC) were 3306, 3696, and 4688, respectively. Biotin was covalently coupled to DBS through the esterification reaction as a new material to prepare nanoparticles. The morphology, particle size, and loading capacity of Bio-DBS nanoparticles were all related to the molecular weights of DBS. The 1 H NMR results indicated that there was a hydrogen bonding interaction between Bio-DBS and resveratrol, which contributed to the photochemical and antioxidant activity of resveratrol in the nanoparticles. The highest encapsulation efficiency (78.9 %) and loading capacity (15.78 %) of resveratrol were observed in Bio-DBS 3 nanoparticles. Additionally, the cell viability was over 80 % when the concentration of Bio-DBS 3 reached to 200 μg/mL. The Bio-DBS nanoparticles significantly improved the thermal stability, photostability, and antioxidant properties of resveratrol. Therefore, the Bio-DBS nanoparticles prepared in this study can be used as a promising carrier to improve the stability and antioxidant activity of resveratrol and may have potential applications in oral delivery.
研究领域:
药学
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纳米技术
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食品科学
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产品关联
DOI
PubMed
高影响因子
Polymers
·
2022
·Vol. 14
·期 17
·3547
·
IF 4.967
Naila Ishaque,Nauman Naseer,Muhammad Asad Abbas,Fatima Javed,Shehla Mushtaq,Nasir M. Ahmad,Muhammad Farhan Ali Khan,Naveed Ahmed,Abdelhamid Elaissari
In this research work, polymer blends of poly-lactic acid (PLA)/ethylene vinyl acetate (EVA) were prepared as the drug carrier materials for a bi-layer drug-loaded coating film for coronary stents. Different optimum compositions of blends were prepared by using an intense mixer. Then, the blends were hot-pressed and later cold-pressed to prepare for films of different thickness. The changes in weight, surface analysis and biodegradability with increasing time were studied using Scanning electron microscopy (SEM), weight loss and biodegradability tests. The mechanical and thermal properties of drug-loaded films were studied through universal testing machine (UTM) and thermo-gravimetric analysis (TGA). The effects of PLA, EVA and drug contents on in-vitro drug contents were investigated through the Ultraviolet-Visible Spectroscopy (UV-VIS) chemical analysis technique. The results obtained clearly showed that the addition of PLA promoted the unleashing of the drug whereas the addition of EVA nearly did not have the same affect. The mechanical properties of these various films can be tuned by adjusting the contents of blend parts. The factors affecting the unleashing of the drug became a serious matter of concern in evaluating the performance of bio-resorbable drug eluting stents. As a result, today’s chemical blends may be useful drug carrier materials for drug-loaded tube coatings capable delivering purgative drug in an incredibly tunable and regulated manner.Keywords:cardiovascular disease (CVD);biodegradable drug eluting stents;polymeric blends
研究领域:
医学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Naijian Ge,Donghui Wang,Feng Peng,Jinhua Li,Yuqin Qiao,Xuanyong Liu
Drug-eluting stents (DESs) are widely used in the palliative treatment of many kinds of cancers. However, the covered polymers used in DESs are usually associated with stent migration and acute cholecystitis. Therefore, developing noncovered drug-loading layers on metal stents is of great importance. In this work, Ni–Ti layered double hydroxide (Ni–Ti LDH) films were prepared on the surface of nitinol via hydrothermal treatment, and the LDH films were further modified by poly(styrenesulfonate) (PSS). The anticancer drug doxorubicin could be effectively loaded onto the modified films, and drug release could be smartly controlled by the pH. Besides, the drug absorption amounts of cancer cells cultured on the films could be effectively improved. These results indicate that the PSS-modified LDH film may become a promising drug-loading platform that can be used in the design of DESs.
研究领域:
材料科学
·
生物医学工程
·
制药学
✓ 已验证
DOI
PubMed
高影响因子
International Journal of Pharmacology
·
2025
·Vol. 21
·期 5
·44208
·
IF 0.2
Rongrong Lu,Aimaitijiang Maimaiti,Tuohetiajimu Abudureheman
Background and Objective:Drug-eluting stents (DES) have become a crucial strategy for improving the outcomes of patients with coronary artery disease (CAD). This work aimed to evaluates the therapeutic efficacy of metoprolol-loaded poly (lactic-co-glycolic acid)–poly(trimethylene carbonate)–poly (glycolic acid) (PLGA–PTMC–PGA) DES (Meto-PLGA/PTMC DES) in a rabbit model of CAD.Methods:A total of 30 New Zealand white rabbits (male, weighing 4–6 kg) were randomly divided into three groups: Sham group (chest suturing without intervention), Model group (CAD animal model without DES), and stent group (treatment with the Meto-PLGA/PTMC DES). The blood samples were collected at regular intervals to assess the serum inflammatory markers, cardiac function parameters, hemodynamic parameters and coronary remodeling. Statistical analysis was performed using One-way analysis of variance (ANOVA), followed by a post hoc Tukey’s test to compare the differences between groups (p < 0.05 indicated statistically significance).Results:The Meto-PLGA/PTMC DES, through its unique co-polymer structure, achieved stable Meto loading in vivo. This stent enabled the gradual release of Meto, thus maintaining sustained drug concentrations and optimizing CAD treatment. The animal experiment results indicated that the sent group (Meto-loaded DES) exhibited markedly lower levels of interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-α), vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1) relative to the model group (p < 0.05). In terms of cardiac function, compared with the model group, the stent group exhibited significantly elevated left ventricular systolic pressure (LVSP), ±dp/dtmax, arterial systolic blood pressure (BPs), and diastolic blood pressure (BPd), along with a marked reduction in left ventricular end-diastolic pressure (LVEDP) compared with the model group (p < 0.05). The coronary tissue morphology in the stent group revealed notably reduced intimal thickness, intimal area and degree of stenosis versus the Model group, with a prominent increase in lumen area (p < 0.05).Conclusion:The Meto-PLGA/PTMC DES not only effectively provides sustained drug release and exhibits superior mechanical properties and excellent blood compatibility. Animal experiments further validated the crucial role of the Meto-PLGA/PTMC DES in reducing inflammatory responses, improving cardiac function and alleviating coronary artery stenosis.
研究领域:
心血管病学
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生物材料学
·
药剂学
·
介入心脏病学
·
炎症生物学
·
转化医学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Shenglin Sun,Yan Hong,Zhengbiao Gu,Li Cheng,Xiaofeng Ban,Zhaofeng Li,Caiming Li
This study aimed to investigate the effects of fatty acid (FA) type on the binding state, fine structure, and digestibility of debranched maize starch (DMS)-FA complexes with different enzymatic debranching degrees. Maize starch was hydrolyzed by pullulanase for 1 h (DMS1h) and 6 h (DMS6h) and then complexed with seven types of FAs with varying chain lengths and unsaturation degrees, respectively. All the DMS-FA complexes showed V 6III -type and B-type crystals. Complex formation greatly increased the relative crystallinity of DMS, but significantly decreased its order degree of short-range structure ( p < 0.05). Compared with unsaturated FAs, saturated FAs possessed stronger intermolecular interactions with DMS. DMS6h-FA complexes exhibited a markedly higher complexing degree ( p < 0.05) than the corresponding DMS1h-FA complexes. The FA molecules in DMS1h-FA complexes were primarily physically trapped outside the amylose helices, whereas those in DMS6h-FA complexes were mainly weakly bound to the cavity of amylose helices. The resistant starch (RS) content and relative crystallinity of DMS-FA complexes considerably increased with increasing FA chain length. Furthermore, the highest RS content (38.90 %) and relative crystallinity (24.23 %) were observed in DMS6h-FA complexes. The FA unsaturation degree induced little effect on the RS content and long-range structural order of the complexes.
研究领域:
食品科学
·
化学
·
营养学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Jing Li,Guangxin Liu,Xinyue Zhu,Mengting Wang,Weiqi Li,Wei Wang,Aijun Hu,Jie Zheng
The effects of different dosage of NaCl and sucrose on the structural and functional properties of debranched quinoa starch-oleic acid complexes (DQS-OA) under baking were investigated. The results showed that the resistant starch content of the baked DQS-OA increased by 17.15 % than DQS-OA. The addition of NaCl destroyed the thermal stability, short-range molecular order and crystalline structure of the complexes. The results of particle size, SEM and amylose content showed that NaCl accelerated the degradation of starch granules, which reduced the enzyme resistance of starch. In contrast, the enthalpy (7.28 J/g-7.78 J/g) and crystallinity (54.29 %–56.69 %) of the samples with sucrose significantly increased, and the molecular structure of the complexes became more ordered. Furthermore, with the increase of sucrose concentration, the resistant starch content also increased from 28.80 % to 31.41 %.
研究领域:
食品科学
·
淀粉化学
·
烘焙技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Bioprinting
·
2022
·
IF 7.422
Chengjin Wang,Yang Yang,Jingyuan Ji,Yongcong Fang,Liliang Ouyang,Lei Zhang,Wei Sun
Intimal hyperplasia and restenosis caused by excessive proliferation of smooth muscle cells (SMC) are the main factors for the failure of stent implantation. Drug-eluting stents carried with antiproliferative drugs have emerged as a successful approach to alleviate early neointimal development. However, these agents have been reported to have an undesirable effect on re-endothelialization. In this study, we proposed an integrated bioresorbable stent coated with dipyridamole (DP)-loaded poly(D,L-lactide) (PDLLA) nanofibers. Three-dimensional (3D) bioresorbable stents were fabricated by printing on a rotation mandrel using polycaprolactone (PCL), and the stents were further coated with PDLLA/DP nanofibers. The in vitro degradation and drug release evaluation illustrated the potential for long-term release of DP. Stents coated with PDLLA/DP nanofibers showed excellent hemocompatibility. The cell viability, proliferation, and morphology analysis results revealed that stents coated with PDLLA/DP nanofibers could prevent the proliferation of SMC and have no adverse effects on endothelial cells. The in vivo implantation of stents coated with PDLLA/DP nanofibers showed initial patency and continuous endothelialization and alleviated neointimal formation. The attractive in vitro and in vivo performance indicated its potential for restenosis prevention and endothelialization.
研究领域:
生物医学工程
·
材料科学
·
心血管医学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioactive Materials
·
2022
·
IF 16.874
Rui Wang,Jian Lu,Jiasheng Yin,Han Chen,Hongmei Liu,Fei Xu,Tongtong Zang,Rende Xu,Chenguang Li,Yizhe Wu,Qilin Wu,Xiang Fei,Meifang Zhu,Li Shen,Junbo Ge
An increased level of reactive oxygen species (ROS) plays a major role in endothelial dysfunction and vascular smooth muscle cell (VSMC) proliferation during in-stent thrombosis and restenosis after coronary artery stenting. Herein, we report an electrospun core-shell nanofiber coloaded with 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPOL) and rapamycin (RAPA) that correspondingly serves as an ROS scavenger and VSMC inhibitor. This system has the potential to improve the biocompatibility of current drug-eluting stent (DES) coatings with the long-term and continuous release of TEMPOL and rapamycin. Moreover, the RAPA/TEMPOL-loaded membrane selectively inhibited the proliferation of VSMCs while sparing endothelial cells (ECs). This membrane demonstrated superior ROS-scavenging, anti-inflammatory and antithrombogenic effects in ECs. In addition, the membrane could maintain the contractile phenotype and mitigate platelet-derived growth factor BB (PDGF-BB)-induced proliferation of VSMCs. In vivo results further revealed that the RAPA/TEMPOL-loaded covered stents promoted rapid restoration of vascular endothelium compared with DES and persistently impeded inflammation and neointimal hyperplasia in porcine models.
研究领域:
生物医学工程
·
药学
·
心脏病学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Science & Nutrition
·
2022
·Vol. 11
·期 1
·481-492
·
IF 3.553
Yongmei Guan,Meichen Wang,Xinqi Song,Shenghang Ye,Cheng Jiang,Huanhuan Dong,Weifeng Zhu
Three different methods, including autoclaving, autoclaving–debranching, and purification, were used to prepare Kudzu-resistant starch (KRS) from Kudzu starch (KS). The physicochemical properties, such as thermodynamic properties, pasting properties, solubility, swelling, and coagulability, as well as the in vitro digestive characteristics of the three kinds of KRS were studied. The results showed that the morphology of starch granules of KRS prepared by autoclave, autoclave enzymatic hydrolysis, and purification methods was changed and the relative crystallinity was significantly decreased compared with the original starch. X-ray diffraction (XRD) showed that KRS exists in the form of C and C+V crystalline form. There was a significant increase in the pasting temperature and a remarkable decrease in the peak viscosity and the expansion degree of the KRS prepared by all three methods. The solubility of the resistant starch (RS) obtained by autoclaving–debranching and that by purification were both increased compared to that of native KS, while the solubility of the RS obtained by autoclaving was decreased. Meanwhile, the retrogradation of the three RS was also improved to varying degrees. The contents of RS in the samples were: P-KRS (71%) > DA-KRS (43%) > A-KRS (42%) > KS (9%). Simulated human in vitro digestion experiments showed that RS has stronger antidigestibility properties than native starch. Among them, the RS prepared by the purification method has stronger antidigestive properties, and it is predicted that it may have a better potential value in regulating blood glucose. These results indicated that the processing properties of KRS, especially the digestibility, are significantly improved and can be used as a new functional food ingredient, which deserves thorough study.
研究领域:
食品科学
·
营养学
·
化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）