JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Ruiwei Xu,Yi Zhang,Yingfeng Gao,Shuyu Jia,Seokho Choi,Yifan Xu,Jicheng Gong
DNA adducts are widely recognized as biomarkers of exposure to environmental carcinogens and associated health effects in toxicological and epidemiological studies. This study presents a targeted and sensitive method for comprehensive DNA adductome analysis using ultra-high-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UHPLC-QqQ-MS/MS). The method was developed using calf thymus DNA, with careful optimization of mass spectrometric parameters, chromatographic separation conditions, and pretreatment methods. Ultimately, a targeted method was established for 41 DNA adducts, which showed good linearity (R 2 ≥0.992), recovery (80.1–119.4 %), accuracy (81.3–117.8 %), and precision (relative standard deviation <14.2 %). The established method was employed to analyze DNA adducts in peripheral blood cells from pregnant women in Shanxi and Beijing. Up to 23 DNA adducts were successfully detected in samples of varying sizes. From 2 μg of maternal DNA samples, seven specific adducts were identified: 5-methyl-2'-deoxycytidine (5-MedC), 5-hydroxymethyl-2'-deoxycytidine (5-HmdC), N 6 -methyl-2'-deoxyadenosine (N 6 -MedA), 8-hydroxy-2'-deoxyguanosine (8-OHdG), 5-hydroxy-2'-deoxycytidine (5-OHdC), 1,N 6 -etheno-2'-deoxyadenosine (1,N 6 -εdA), and N 2 -methyl-2'-deoxyguanosine (N 2 -MedG). This study reveals that exposure to higher concentrations of ambient air pollutants may elevate the levels of DNA methylation and oxidative damage at different base sites, highlighting the application potential of DNA adducts as sensitive biomarkers of air pollution exposure.
研究领域:
环境健康
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毒理学
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流行病学
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分析化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Jinsong Zhang,Youzhi Zhou,Ke Liu,Jianlin Chu,Yewei Zhang,Bingfang He
A novel nucleoside 2′-deoxyribosyltransferase ( Lh NDT) from Lactobacillus hilgardii ZJS01 was cloned and expressed in Escherichia coli BL21 (DE3). The amino acid sequence of Lh NDT displayed the highest identity of 26% to other reported NDTs. Lh NDT was a monomer as revealed by gel filtration chromatography with or without addition of Ca 2+ in elution buffer. This enzyme showed a high activity and stability over a broad pH range and was sensitive to temperature. With the addition of 15 mM Ca 2+ , the thermostability of Lh NDT was greatly promoted by 63 times and 46 times at 30 °C and 35 °C, respectively. With the use of 2′-deoxyuridine as sugar donor at an economic molar ratio of 1:1, 5-aza-2′-deoxycytidine (46%) and 5-fluoro-2′- deoxycytidine (58%) were efficiently synthesized from 5-azacytosine and 5-fluorocytosine, respectively, by Lh NDT for 2 h.
研究领域:
酶学
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生物技术
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CATALYSIS
·
2025
·
IF 6.5
Ting-Sheng Chen,Di Liu,Yun-Jie Li,Xing-Bao Wang,Chang-Hai Liang,Zhen-Yi Du,Wen-Ying Li
Controlling the oxygen-free product selectivity in catalytic hydrodeoxygenation is essential for producing high quality fuels from coal- and biomass-derived crude liquids. A series of boron-doped Ni/SiO 2 catalysts were prepared for the efficient hydrodeoxygenation of dibenzofuran as a model compound. Boron doping significantly promoted the transformation of 2-cyclohexylcyclohexanol, which was accumulated as the main oxygen-containing intermediate compound on the pristine Ni/SiO 2 catalyst, to the target products bicyclohexane and its isomer cyclopentylmethylcyclohexane. Characterization results show that boron doping increases Ni dispersion, modifies the electronic properties of Ni sites and in situ generates Brønsted acid sites by H 2 spillover to boron oxides. The reduced Ni particle size improves the rates of dibenzofuran hydrogenation and 2-cyclohexylcyclohexanol dehydration. Electron-deficient Ni and boron oxides as the Lewis acid sites enhance the adsorption of 2-cyclohexylcyclohexanol. Furthermore, the in situ generated Brønsted acid sites favor the dehydration of 2-cyclohexylcyclohexanol, which gives the Ni-5B/SiO 2 catalyst a better deoxygenation activity than the mechanical mixture of equal amounts of 5B/SiO 2 and Ni/SiO 2 , as well as the one with a smaller Ni particle size of 3.8 nm.
研究领域:
催化化学
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能源化学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Mengzhe Guo,Liyan Zhang,Yan Du,Wencheng Du,Dantong Liu,Cheng Guo,Yuanjiang Pan,Daoquan Tang
5-(Hydroxymethyl)-2′-deoxycytidine (5-hmdC), 5-(formyl)-2′-deoxycytidine (5-fodC), and 5-(carboxyl)-2′-deoxycytidine (5-cadC) are crucial intermediate products of the DNA demethylation pathway, which can also act as potential biomarkers reflecting the diagnosis and prognosis in multiple tumors. Detecting 5-hmdC, 5-fodC, and 5-cadC in human urine has various advantages including readily available samples and being noninvasive to patients. However, few works have reported the detection of 5-fodC and 5-cadC due to their trace amounts. Here we developed a novel magnetic hyper-cross-linked microporous polymer (HMP) material based on polyionic liquid (PIL) for the enrichment of 5-hmdC, 5-fodC, and 5-cadC. These magnetic PIL-HMP materials provided specific enrichment superiority for three modified cytidines. After enrichment, the signal intensity of 5-hmdC, 5-fodC, and 5-cadC increased 10–75-fold with lower limits of quantitation (LLOQ) of 0.049, 0.781, and 0.781 ng/mL, respectively. The recoveries were approximately 86.5–95.2% for 5-hmdC, 95.2–107.8% for 5-fodC, and 99.4–102.4% for 5-cadC under the relative standard deviation (RSD) of 0.2–10.3%. Finally, we successfully applied magnetic PIL-HMP materials coupled with high-performance liquid chromatography–electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) in enrichment and quantitative determination of 5-hmdC, 5-fodC, and 5-cadC in human urine of 10 breast cancer patients and 10 healthy people. We found that the level of 5-hmdC decreased in breast cancer patients (p < 0.05), while the levels of 5-fodC and 5-cadC increased (p < 0.05, p < 0.01). Our results demonstrated that the levels of metabolic 5-hmdC, 5-fodC, and 5-cadC in human urine are closely related to breast cancer, which could contribute to the clinical diagnosis and investigation of breast cancer and its occurrence and development mechanisms.
研究领域:
医学
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分析化学
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2022
·
IF 6.371
Mingyuan Zhang,Taotao Cai,Weiliang Lu,Shili Zheng,Ying Zhang,Yi Zhang
Given the high cost of fabricating the Ti-6Al-4V alloy powder by the traditional hydriding-dehydriding and atomization methods, a novel sintering-deoxygenation process was proposed and studied in this research. With the help of the developed deoxygenation treatment, value-added raw materials such as Ti sponge can be substituted by low-cost and low-grade materials with high oxygen content. In addition, sintering in a solid state saves a significant amount of processing energy compared to alloying above the melting point of Ti in the conventional alloy production process. This paper prepared six sintering precursors with different initial compositions and oxygen contents using the low-valued raw materials of crude titanium, crude vanadium, V 2 O 3 , Al powder, and Al 2 O 3 powder. These precursors' sintering performances on shrinkage, phase composition, microstructure, and porosity were investigated. The results show that the sintering shrinkage is positively correlated with the sintering temperature and holding time and is also affected by the raw materials. The pellet made by mixed crude titanium and AlV master alloy showed the largest shrinkage and the densest bulk. The various combinations of the raw materials led to the changes in the oxygen contents in the precursors, the alpha-beta phase ratio, and the shift of the diffraction peaks of the α-phase. The dissolving of the AlV master alloy and the formation of the Ti 3 Al intermediate phase took place during sintering due to diffusion. Sintering at 900–1000 °C is critical for elemental diffusion and densification, and sintering at higher temperatures is decisive for microstructure homogenization and further densification. Using the hydrogen-assisted Mg deoxygenation method, all the sintered samples could be treated to an oxygen level of around 0.4 wt% regardless of oxygen content in precursors. The contents of Ti, Al, V, and Fe in the obtained Ti-6Al-4V powders met the requirements of GB/T 34486-2017. This research has demonstrated the feasibility of fabricating Ti-6Al-4V alloy powder by the novel sintering-deoxygenation process.
研究领域:
材料科学
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冶金工程
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产品关联
DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Ting Huang,Guixun Chen,Birong Liu,Zizhong Yang,Yuanwei Huang,Baoping Xie,Min-Min Li,Jin-Xiang Chen,Jun Chen,Zong Dai
Sensitive, specific and rapid methods for detecting microRNAs (miRNAs) play critical roles in disease diagnosis and therapy. Enzyme-free amplification techniques based on DNAzyme assembly have recently been developed for the highly specific miRNA analysis. However, traditional DNAzyme-based assembly (free DNAzyme) amplifiers is mainly dependent on the target-induced split DNAzyme fragments to assemble into activated DNAzyme structures, which have made a compromise between the sensitivity and specificity due to the random diffusion of dissociative probes in a bulk solution with poor kinetics. Herein, based on a rationally designed DNA probe , we developed an intramolecular DNAzyme assembly (intra-DNAzyme) method to overcome these challenges. The miR-373 is used as model analyte for our current proof-of-concept experiments. Compared with the free-DNAzyme method, our method showed significantly improved analytical performance in terms of dynamic range, assay sensitivity and speed. This method can detect miR-373 specifically with a detection limit as low as 4.3 fM, which is about 83.7 times lower than the previous free-DNAzyme method. This intra-DNAzyme strategy would be of great value in both basic research and clinical diagnosis.
研究领域:
分子生物学
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生物技术
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纳米技术
·
诊断学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CATALYSIS
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2022
·
IF 8.047
Han He,Shuqian Xia,Dan Luo
Refining lignin for aromatic hydrocarbons was crucial in the development of clean energy. The most effective way to enhance the carbon efficiency of the raw materials was to reduce the hydrogen consumption and maximize the retention of aromatic ring by directly breaking the C Ar O bond. In this regard, Sn was introduced to regulate the selectivity of Ni/SiO 2 for m-cresol hydrodeoxygenation (HDO) products at low hydrogen consumption. Thanks to the formation of the Ni-Sn alloy in the system, the bimetallic sites synergistically catalyze the C Ar O bond scission through lowering the reaction energy barrier. Further from charge analysis, it was concluded that the charge transfer from Sn to Ni promotes the hydrogenolysis, and the charge transfer between the slab and the adsorbate provides the possibility for the occurrence of direct deoxygenation reaction. The COHP analysis resulted that the introduction of Sn accelerated the C Ar O bond cleavage. Interestingly, another deoxygenation pathway was proposed from the isomerization conversion of m-cresol to dienone intermediate (3-methyl-3,5-cyclohexadienone) in the calculation of transition state. Further HDO can form toluene effortlessly due to the presence of the oxyphilic metal Sn. This also explains the high selectivity of Ni-Sn to toluene. Moreover, the introduction of the Sn promoter effectively prolongs the lifetime of the catalyst by enhancing the tolerance to carbon deactivation.
研究领域:
化学工程
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催化科学
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可再生能源
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产品关联
DOI
高影响因子
FUEL PROCESSING TECHNOLOGY
·
2020
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IF 4.982
Kuan Ding,Hao Zhou,Yiling Wan,Yong Huang,Yuming Zhang,Xun Hu,Hong Zhang,Shu Zhang
Ketonization of biomass-derived oxygenates provides an efficient way for bio-oil upgrading. However, little information on the ketonization of oxygen-rich sugars has been reported, leaving the deep deoxygenation of biomass an unsolved challenge. To address the problem, this paper investigated the catalytic pyrolysis of xylose over nano-CeO 2 using an analytical pyrolyzer . Results showed that upon the addition of CeO 2 , multi-functional oxygenated compounds from xylose pyrolysis were majorly reformed to mono-functional ketonic products, resulting in a 5-fold increase in peak area of ketones . Among them, acetone and 2-butanone presented the highest selectivity of 51% with the CeO 2 to xylose ratio of 10:1 at the temperature of 600 °C. Meanwhile, the short-chain oxygenates, i.e. C 2 -C 3 aldehydes and alcohols, had been converted into C 3 -C 5 ketones, indicating an outstanding ability of chain-increasing over CeO 2 . Both the catalyst dosage and the pyrolysis temperature play a vital role during the ketonization process. The reaction mechanism was proposed according to the experimental results, in which three major reaction pathways were analyzed. This study demonstrates that ketonization of biomass-derived sugars is a promising method for oxygen removal and carbon chain increase, thus improving the bio-oil quality.
研究领域:
生物质能
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催化化学
✓ 已验证
产品关联
DOI
高影响因子
Catalysts
·
2023
·Vol. 13
·期 5
·878
·
IF 3.9
Yue Huang,Renhao Si,Yi Wei
Lignin is a crucial raw material for the synthesis of phenol due to its high oxygen concentration; however, the selectivity of phenol in pyrolysis products is low. This study chose cerium oxide as the coupling agent for molten salts, and the reaction’s process conditions—including temperature and catalyst dosage—were examined. The influence of metal loading on the reaction and byproducts of catalytic pyrolysis was examined. The outcomes demonstrated that the best monophenol production was accomplished at 550 °C and an Fe loading of 15 wt.%, with 93.93 wt.% monophenols in the bio-oil. While methoxy was more easily broken and rearranged, producing more monophenols, the presence of the catalyst enhanced the oxidation of Cα-OH and the breaking of β-O-4 bonds, which is significant for the study of lignin pyrolysis.Keywords:molten carbonates;pyrolysis;deoxidation;monophenols;lignin
研究领域:
化学工程
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生物质能研究
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Yong-Qiang He,Yu Gao,Hui-Wen Gu,Xian-Zhu Meng,Hong-Chao Yi,Ying Chen,Wei-Yin Sun
In this work, a sensitive electrochemical method for bleomycin (BLM) determination was reported on the basis of BLM-mediated activation of Zn 2+ -dependent DNAzyme and the adsorption of signal probes by a metal-organic framework (MOF) modified electrode . Two hairpin DNAs were employed in this protocol, one (HP1) for BLM recognition and one (HP2) for amplified signal output. The presence of BLM and Fe 2+ caused the formation of BLM-Fe (II) complex to cleave HP1, releasing DNAzyme fragments, which could further hybridize with substrate HP2 to form a partial double-stranded DNA duplex and enable the activation of Zn 2+ -dependent DNAzyme with the coexistence of Zn 2+ . The Zn 2+ -dependent DNAzyme catalyzed the cyclic cleavage of magnetic beads (MB)-immobilized HP2 to release massive DNA fragments with a Fc-labeled- terminal, which could be used for BLM quantification through electrochemical measurement after their adsorption on a MOF modified electrode. Attributing to the high catalytic efficiency of DNAzyme and excellent electrochemical performance of MOF modified electrode, our method revealed an impressive limit of detection as low as 4 pM BLM with a linear range of 5–2000 pM. Besides, the easy synthesis of MOF without further modification and the easy way of adsorption for signal achievement facilitated the operation process. In virtue of the high sensitivity, selectivity and the simple-to-implement features, this method is believed to hold a great promising application for BLM determination in biomedical and clinical study.
研究领域:
分析化学
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生物化学
·
生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Lan Luo,Fengzhou Xu,Hui Shi,Xiaoxiao He,Taiping Qing,Yanli Lei,Jinlu Tang,Dinggeng He,Kemin Wang
Deoxyribonuclease I (DNase I) is an important physiological indicator and diagnostic biomarker, but traditional methods for assessing its activity are time-consuming, laborious, and usually radioactive. Herein, by effectively combining the special functions of DNase I and terminal deoxynucleotidyl transferase (TdT), a simple, green, cost-effective, label-free and ultrasensitive assay for DNase I activity has been constructed based on superlong poly(thymine)-hosted copper nanoparticles (poly T-CuNPs). In this strategy, a 3′-phosphorylated DNA primer is designed to block TdT polymerization. After addition of DNase I, the primer could be digested to release 3′-hydroxylated fragments, which could further be tailed by TdT in dTTP pool with superlong poly T ssDNA for CuNPs formation. Fluorescence measurements and gel electrophoresis demonstrated its feasibility for DNase I analysis. The results indicated that with a size of 3–4 nm, the CuNPs templated by TdT-polymerized superlong poly T (>500 mer) had several advantages such as short synthetic time (<5 min), large Stokes shift (~275 nm) and intense red fluorescence emission . Under the optimal conditions, quantitative detection of DNase I was realized, showing a good linear correlation between 0.02 and 2.0 U/mL (R 2 =0.9928) and a detection limit of 0.02 U/mL. By selecting six other nucleases or proteins as controls, an excellent specificity was also verified. Then, the strategy was successfully applied to detect DNase I in diluted serum with a standard addition method, thus implying its reliability and practicability for biological samples. The proposed strategy might be promising as a sensing platform for related molecular biology and disease studies.
研究领域:
生物化学
·
分子生物学
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医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Yan Shi,Wei Xu,Dawei Ni,Wenli Zhang,Cuie Guang,Wanmeng Mu
Deoxynivalenol (DON) poses a significant threat to human health due to its widespread distribution and biological toxicity. Here, we identified a novel DON-degrading enzyme from Youhaiella tibetensis ( Yo DDH). Yo DDH exhibited the highest activity against DON at pH 4.5 and 40 ℃, in the presence of Ca 2+ and the pyrroloquinoline quinone (PQQ). Additionally, Yo DDH displayed remarkable thermostability at 40 ℃, with a half-life of 24 h and a T m value of 48.5 ℃. Notably, phenazine methosulfate (PMS) and 2,6-dichlorophenolindophenol (DCPIP) can also serve as electron acceptors for Yo DDH. After incubation in the optimal conditions for 3 h, Yo DDH degraded 73 % of DON (100 μM) finally. The k cat and k cat / K m of Yo DDH towards DON was determined as 1.65 s −1 and 1526 M −1 ·s −1 in the presence of PMS. The 3-keto-DON was verified as the degradation product. This identified Yo DDH presents a promising candidate for DON decontamination in the food and feed industry.
研究领域:
食品安全
·
微生物学
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）