CARBOHYDRATE POLYMERS
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2022
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IF 10.723
Qingxia Yuan,Hong Li,Qian Wang,Shujing Sun,Ziyu Fang,Hao Tang,Xiaohuo Shi,Jing Wen,Lianghua Huang,Meng Bai,Shengping Zhong,Yonghong Liu,Chenghai Gao,Longyan Zhao
Stichopus monotuberculatus is a tropical sea cucumber species and used as a folk medicine and tonic food . In this study, a fucosylated glycosaminoglycan (SmFG), the depolymerized SmFG (dSmFG) and its oligosaccharide fractions were prepared. The SmFG and its depolymerized products were comprised of a chondroitin-sulfate-E backbone, and various sulfated fucose side chains, including an unusual disaccharide side chain connected to the C-3 position of D-glucuronic acid (GlcA) or GlcA-ol. A peeling reaction occurred during the deaminative depolymerization process. The dSmFG and its fractions showed strong anticoagulant activity by selectively inhibiting intrinsic tenase complex, and had no anti-factor IIa, Xa and VIIa activity. The anticoagulant activity reduced with the decrease of molecular weight , and the unusual branch and novel reducing end may enhance the anticoagulant activity. These findings can provide significant information for development and utilization of depolymerized products from SmFG in food and pharmaceutical industries .
研究领域:
海洋生物学
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药理学
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食品科学
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DOI
PubMed
高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2021
·
IF 4.219
Feng Li,Liping Wang,Yilei Cai,Yihuo Luo,Xianai Shi
In this work, the acute and subchronic toxicities of desaminotyrosine (DAT) by oral administration in SD rats and its effects on the intestinal microflora were investigated. The acute toxicity test showed that DAT is a low-toxic substance with a LD 50 of 3129 mg/kg. The subchronic toxicity test showed that DAT has no toxicity at a low dose (125 mg/kg/day). However, DAT exhibited obvious toxicities to food intake, liver, kidney, and lung at higher dose (250 mg/kg/day and 500 mg/kg/day). DAT inhibited the food intake of rats in a dose-dependent manner. Serum biochemical analysis showed that DAT can increase the serum glucose level of rats. Fecal microbiota analysis showed that DAT treatment can significantly change the intestinal microflora of rats, the dose of 125 mg/kg/day has the most significant effect on the diversity of intestinal microbiota. In daily application, the side effects caused by DAT might be gastrointestinal irritation , weight loss, liver or kidney injury , and blood sugar elevation. Based on our study, the no-observed-adverse-effect level (NOAEL) of DAT is 125 mg/kg BW/day for rats.
研究领域:
毒理学
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微生物学
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营养学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
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IF 5.7
Weijie Gao,Zijian Jing,Yifang Meng,Qinghai Liu,Hualei Wang,Dongzhi Wei
Lysine cyclodeaminase (LCD)-mediated synthesis of l-pipecolic acid (l-PA) from l-lysine (l-Lys) is a promising approach. However, only one LCD has been reported, and its inadequate activity limits industrial applications. To address this problem, a substrate analogue-guided enzyme mining strategy was employed. A novel ornithine cyclodeaminase (OCD) from Rhizobium leguminosarum (RlOCD) was identified in combination with directed macrogenomic approaches. RlOCD displayed a conversion rate of 28% at a substrate loading as high as 1000 mM. A multiregion synergy strategy consisting of pocket reshaping, dynamical cross-correlation matrix-guided coevolutionary design, and surface modification was used to design RlOCD from the inside-out. A quadruple mutant (V93C/L119C/I170T/R90L) designated Mu4 with significantly increased activity was obtained, which showed a 28.46-fold increase in the catalytic efficiency. The conversion of Mu4 was 91% within 10 h at 1000 mM (146.19 g L–1) loading. The space–time yield of 282.1 g L–1 d–1 is the highest level ever reported. Molecular dynamics simulations and interaction analyses revealed that efficient pocket expansion and unique conformational rearrangements increased the affinity for the substrate, resulting in a more catalytically active conformation. This study expands the toolbox for the production of l-PA and demonstrates the effectiveness and potential of Mu4 for its production.
研究领域:
生物化学
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酶工程
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合成生物学
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DOI
PubMed
高影响因子
Advanced Science
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2025
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IF 14.1
Heng Du,Xu Liu,Jianghua Shen,Hailong Yuan,Hao Zhang,Gan Xi,Yujing Li,Yuhan Wang,Jiahe Zhang,Chaofan Yang,Pengfei Xu,Jiawan Wang,Fang Wang,Siqi Liu,Yanan Zhou,Qi Gu,Jingjing Lu,Tuo Wei,Zeyu Gao,Jingyi Zang,Jun Wang,Moshi Song
Myocardial ischemia/reperfusion (I/R) injury is a major contributor to myocardial damage, leading to adverse cardiac remodeling and dysfunction. Recent studies have highlighted the potential of gut microbiota-derived metabolites in modulating cardiac outcomes. Here, the cardioprotective effects of a commensal bacterium Flavonifractor plautii ( F. plautii ) and its metabolite desaminotyrosine (DAT) against myocardial I/R injury are investigated. We showed that prophylactic gavage of F. plautii attenuates myocardial I/R injury as evidenced by improved cardiac function and reduced cardiac injury. We also found that its metabolite DAT recapitulates these cardioprotective effects against myocardial I/R injury. Transcriptomic analysis has revealed that DAT preserves cardiac tissue and attenuates immune responses against myocardial I/R injury. Mechanistically, DAT promotes cardiomyocyte survival through the modulation of the nicotinamide adenine dinucleotide phosphate (NADP + /NADPH) ratio. Further, DAT suppressed macrophage proinflammatory activities and cardiac inflammation via the reduction in interleukin-6 (IL-6) production. Taken together, our findings indicate that F. plautii and its metabolite DAT exert pleiotropic cardioprotective effects against myocardial I/R injury, suggesting them as potential prophylactic therapeutic options for alleviating myocardial I/R injury.
研究领域:
心脏病学
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微生物学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
Inorganic Chemistry Frontiers
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2024
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IF 6.1
Yunhu Wang,Fangxu Dai,Yafei Tao,Kai Zhang,Bingjie Li,Mingming Zhang,Kang Liu,Jixiang Xu,Lei Wang,Jun Xing
Aromatic carbon nitride (PhCN), as an emerging semiconductor material, has attracted much attention due to its excellent optical properties. Fully understanding the synthesis mechanism, chemical structure, and optical properties of PhCN would significantly promote its progress in synthesis, modifications and applications. Here the chemical structures and optical properties of PhCN and intermediates have been systematically studied, and hereby the synthesis mechanism of PhCN is explicitly proposed for the first time. The results show that the precursor 2,4-diamino-6-phenyl-1,3,5-triazine (DPT) is deaminated to form the DPT dimer, trimer and polymers, and then the triazine was isomerized to tri-s-triazine by a ring-opening isomerization reaction to form PhCN. Meanwhile, the correlation between structural and optical properties of PhCN and intermediates demonstrates the electron delocalization effect of the phenyl group and reveals that highly polymerized PhCN has a more stable molecular structure, thus exhibiting more stable photophysical properties. Our work provides a fundamental study from synthesis to structures and optical properties of PhCN, which will certainly promote its deeper research and wider application.
研究领域:
材料科学
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化学
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光学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
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2017
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IF 6.32
Huan Zeng,Chu-Bo Qi,Ting Liu,Hua-Ming Xiao,Qing-Yun Cheng,Han-Peng Jiang,Bi-Feng Yuan,Yu-Qi Feng
5-Methylcytosine (5-mC) is an important epigenetic mark that plays critical roles in a variety of cellular processes. To properly exert physiological functions, the distribution of 5-mC needs to be tightly controlled in both DNA and RNA. In addition to methyltransferase-mediated DNA and RNA methylation, premethylated nucleotides can be potentially incorporated into DNA and RNA during replication and transcription. To exclude the premodified nucleotides into DNA and RNA, endogenous 5-methyl-2′-deoxycytidine monophosphate (5-Me-dCMP) generated from nucleic acids metabolism can be enzymatically deaminated to thymidine monophosphate (TMP). Therefore, previous studies failed to detect 5-Me-dCMP or 5-methylcytidine monophosphate (5-Me-CMP) in cells. In the current study, we established a method by chemical labeling coupled with liquid chromatography–electrospray ionization mass spectrometry (LC–ESI-MS/MS) for sensitive and simultaneous determination of 10 nucleotides, including 5-Me-dCMP and 5-Me-CMP. As N,N-dimethyl-p-phenylenediamine (DMPA) was utilized for labeling, the detection sensitivities of nucleotides increased by 88–372-fold due to the introduction of a tertiary amino group and a hydrophobic moiety from DMPA. Using this method, we found that endogenous 5-Me-dCMP and 5-Me-CMP widely existed in cultured human cells, human tissues, and human urinary samples. The presence of endogenous 5-Me-dCMP and 5-Me-CMP indicates that deaminases may not fully deaminate these methylated nucleotides. Consequently, the remaining premethylated nucleosides could be converted to nucleoside triphosphates as building blocks for DNA and RNA synthesis. Furthermore, we found that the contents of 5-Me-dCMP and 5-Me-CMP exhibited significant decreases in renal carcinoma tissues and urine samples of lymphoma patients compared to their controls, probably due to more reutilization of methylated nucleotides in DNA and RNA synthesis. This study is, to the best of our knowledge, the first report for detecting endogenous 5-Me-dCMP and 5-Me-CMP in mammals. The detectable endogenous methylated nucleotides indicate the potential deleterious effects of premodified nucleotides on aberrant gene regulation in cancers.
研究领域:
生物化学
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表观遗传学
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分子生物学
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癌症研究
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2022
·
IF 8.008
Jun Xiong,Ke-Ke Chen,Neng-Bin Xie,Tong-Tong Ji,Si-Yu Yu,Feng Tang,Conghua Xie,Yu-Qi Feng,Bi-Feng Yuan
DNA methylation (5-methylcytosine, 5mC) is the most prevalent epigenetic modification that is predominantly found in CG dinucleotides in mammalian genomes. In-depth investigation of the functions of 5mC heavily relies on the quantitative measurement of 5mC at single-base resolution in genomes. Here, we proposed a methyltransferase-directed labeling with APOBEC3A (A3A) deamination sequencing (MLAD-seq) method for the single-base resolution and quantitative detection of 5mC in DNA. In MLAD-seq, a mutant of DNA methyltransferase, M.MpeI-N374K, is utilized to selectively transfer a carboxymethyl group to the 5 position of cytosine in the CG dinucleotide to form 5-carboxymethylcytosine (5camC) using carboxy-S-adenosyl-l-methionine (caSAM) as the cofactor. After A3A treatment, 5camC is resistant to the deamination and base pairs with guanine. Thus, the cytosines in CG sites are read as C in sequencing. On the contrary, the methyl group in 5mC inhibits its carboxymethylcytosine by M.MpeI-N374K and therefore is readily deaminated by A3A to produce thymine that pairs with adenine and is read as T in sequencing. The differential readouts from C and 5mC in the MLAD-seq enable the single-base resolution mapping of 5mC in CG sites in DNA. With the developed MLAD-seq method, we observed the hypermethylation in the promoter region of retinoic acid receptor β (RARB) gene from human nonsmall cell lung tumor tissue. Compared to harsh reaction conditions in bisulfite sequencing that could lead to significant degradation of DNA, the whole procedure of MLAD-seq is carried out under mild conditions, which will avoid DNA damage. Thus, MLAD-seq is more suitable in the scenario where only limited input DNA is available. Taken together, the MLAD-seq offers a valuable tool for bisulfite-free, single-base resolution and quantitative detection of 5mC in limited DNA.
研究领域:
遗传学
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表观遗传学
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分子生物学
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癌症研究
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Meng-Yuan Li,Run-Dong Zhang,Yi-Xing Lin,Qi-Wen Li,Qiu-Yue Zhao,Zong-Xi Zhao,Zhong-Ming Ling,Long-Fei Shu,Min Zhang,Li-Xin Hu,Yi-Jing Shi,Guang-Guo Ying
The biotransformation of sulfamonomethoxine (SMM) was studied in an aerobic granular sludge (AGS) system to understand the role of sorption by microbial cells and extracellular polymeric substances (EPS) and the role of functional microbe/enzyme biodegradation. Biodegradation played a more important role than adsorption, while microbial cells covered with tightly bound EPS (TB-EPS) showed higher adsorption capacity than microbial cells themselves or microbial cells covered with both loosely bound EPS (LB-EPS) and TB-EPS. The binding tests between EPS and SMM and the spectroscopic analyses (3D-EEM, UV–Vis, and FTIR) were performed to obtain more information about the adsorption process. The data showed that SMM could interact with EPS by combining with aromatic protein compounds, fulvic acid-like substances, protein amide II, and nucleic acids . Batch tests with various substances showed that SMM removal rates were in an order of NH 2 OH (60.43 ± 2.21 μg/g SS) > NH 4 Cl (52.96 ± 0.30 μg/g SS) > NaNO 3 (31.88 ± 1.20 μg/g SS) > NaNO 2 (21.80 ± 0.42 μg/g SS). Hydroxylamine and hydroxylamine oxidoreductase (HAO) favored SMM biotransformation and the hydroxylamine-mediated biotransformation of SMM was more effective than others. In addition, both ammonia monooxygenase (AMO) and CYP450 were able to co-metabolize SMM. Analysis of UPLC-QTOF-MS indicated the biotransformation mechanisms, revealing that acetylation of arylamine, glucuronidation of sulfonamide, deamination, SO 2 extrusion, and δ cleavage were the five major transformation pathways. The detection of TP202 in the hydroxylamine-fed Group C indicated a new biotransformation pathway through HAO. This study contributes to a better understanding of the biotransformation of SMM.
研究领域:
环境科学
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微生物学
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生物技术
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化学工程
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产品关联
DOI
PubMed
高影响因子
RESEARCH ON CHEMICAL INTERMEDIATES
·
2023
·Vol. 49
·期 10
·4257-4274
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IF 3.3
Cao Wenbo,Ren Haitao,Cai Chaochen,Li Dashuai,Li Tingting,Lou Chingwen,Lin Jiahorng
Photocatalysis is a promising technology for wastewater treatment. It is a key problem to develop photocatalytic materials that can efficiently remove tetracycline (TC) under weak light intensity. This article synthesized the UiO-66-NH 2 /Bi 2.15 WO 6 heterojunction using a solvothermal method, and its specific surface area increases by 2.1 times relative to Bi 2.15 WO 6 , endowing good adsorption capacity for TC. Compared with Bi 2.15 WO 6 , the heterojunction effectively improves the separation of electrons and holes and enhances the photocatalytic performance. Under weak light intensity (4 mW cm −2 ), the removal percentage of 50 mg L −1 TC can reach 78.0%, attributing to a synergistic effect between adsorption and photocatalysis. The free radical capture experiment showed that TC was photodegradation mainly under the action of hole (h + ) and superoxide radical (·O 2 − ). Finally, through LC/MS analysis, it is inferred that TC is degraded into H 2 O and CO 2 after a series of reactions such as deamination, dehydroxylation, amide bond breaking and ring opening. This study provides insights for the synthesis of more powerful photocatalysts for TC removal.
研究领域:
环境科学
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材料科学
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化学工程
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水处理
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）