Polymers
·
2022
·Vol. 14
·期 7
·1333
·
IF 4.967
Jiatian Zhu,Yuqi Bao,Luxiong Lv,Fanyan Zeng,Dasong Du,Chen Liang,Jiayan Ge,Shuangfei Wang,Shuangquan Yao
The preparation of bio-oil through biomass pyrolysis is promoted by different demineralization processes to remove alkali and alkaline earth metal elements (AAEMs). In this study, the hydrothermal pretreatment demineralization was optimized by the response surface method. The pretreatment temperature, time and pH were the response elements, and the total dissolution rates of potassium, calcium and magnesium were the response values. The interactions of response factors for AAEMs removal were analyzed. The interaction between temperature and time was significant. The optimal AAEMs removal process was obtained with a reaction temperature of 172.98 °C, time of 59.77 min, and pH of 3.01. The optimal dissolution rate of AAEMs was 47.59%. The thermal stability of eucalyptus with and without pretreatment was analyzed by TGA. The hydrothermal pretreatment samples exhibit higher thermostability. The composition and distribution of pyrolysis products of different samples were analyzed by Py-GC/MS. The results showed that the content of sugars and high-quality bio-oil (C6, C7, C8 and C9) were 60.74% and 80.99%, respectively, by hydrothermal pretreatment. These results show that the removal of AAEMs through hydrothermal pretreatment not only improves the yield of bio-oil, but also improves the quality of bio-oil and promotes an upgrade in the quality of bio-oil.Keywords:eucalyptus;demineralization;hydrothermal pretreatment;thermostability;pyrolysis products
研究领域:
能源植物学
·
生物质能工程
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Chaoran Yu,Yao Shen,Ce Bian,Miao Liu,Michael D. Weir,Hockin H. K. Xu,Zhiyi Yao,Yuxing Bai,Ning Zhang
Enamel demineralization is a common complication of orthodontic treatment. Studies related to enamel demineralization are usually conducted in the laboratory using in vitro models. Detection technology relies on large instruments, which are not conducive to its popularization and promotion. We designed a fluorescence sensing system using a complex formed by 3,4,9,10-tetra-(4-trimethylaminohexyloxy-carbonyl)-perylene (PDI-HTMA) and casein phospho-peptides (CPPs) that can rapidly detect calcium ions in enamel. In this study, we investigated the feasibility of using PDI-HTMA/CPPs as probes for detecting enamel demineralization. We used Ca 2+ from enamel demineralization as the detection target for the fluorescent probe and verified its stability, specificity, and linear range. This method exhibits strong linear relationships in the Ca 2+ concentration range of 30–500 μM, with a limit of detection of 3.89 μM. The PDI-HTMA/CPPs were applied to human enamel specimens. We compared the detection results with those of existing enamel demineralization evaluation methods, and the results showed a trend consistent with that of ICDAS grading and laser fluorescence caries detection. This fluorescent probe assay is expected to be applied for the early detection of enamel demineralization, which has strong application advantages. To the best of our knowledge, this is the first fluorescent probe assay applied for the in vitro detection of enamel demineralization.
研究领域:
口腔医学
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材料科学
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生物化学
✓ 已验证
DOI
高影响因子
ACS Omega
·
2025
·
IF 4.3
Jing Ru,Xiaochen Xu,Yuxuan Cheng,Nan Luo,Shuo Tan,Xi Chen,Feng Chen,Bing-Qiang Lu
Dental minerals are in an equilibrium state of demineralization and remineralization, which can be disrupted by pathogenic bacteria to cause dental caries. While the inorganic polymer polyphosphate (polyP) is ubiquitous in living organisms and is also widely involved in mineralization regulations, its specific influence on the mineralization balance of teeth remains unclear. As a concept-and-proof study, the effects of polyP on the demineralization and remineralization of teeth are investigated on dental enamel (the highly mineralized outer covering tissue of teeth) from the perspective of mineralization balance. We found that a high concentration (containing 1.0–20 mM P element, comparable to and higher than the free phosphate ions in body fluids) of polyP has the capability to demineralize enamel in the aqueous solution, yet this effect is absent in the simulated biological environments including simulated body fluid and MEM (α-minimum essential medium) solutions. More importantly, polyP with a very low concentration (containing ≥5.0 μM P) is able to inhibit enamel mineralization significantly. This suggests that polyP could impact the mineralization balance of enamel by preferentially inhibiting the remineralization process, thereby disrupting the equilibrium necessary for maintaining enamel health.
研究领域:
口腔医学
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生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DENTAL MATERIALS
·
2022
·
IF 5.687
Yaning Zhao,Jian Yu,Jingmei Guo,Hongye Yang,Cui Huang
Objective To investigate the effects of a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner on dentin bonding durability and explore the possible mechanisms. Methods The SCMC-based extrafibrillar demineralization conditioner was facilely developed by dissolving SCMC into deionized water at an appropriate concentration. A single layer collagen mineralization/demineralization model was designed to visualize extrafibrillar demineralization in detail. Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA). The morphology, composition, and mechanical properties of conditioned dentin from each group were characterized. To evaluate dentin bonding performance, SCMC- and PA- conditioned dentin were applied with adhesive restoration using the dry-bonding technique. The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging. The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated. Results After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure . The mechanical properties of SCMC-conditioned dentin were largely preserved. Compared with PA, SCMC conditioning produced greater MTBS values and less nanoleakage expression after aging. Endogenous gelatinolytic activity was suppressed in SCMC-conditioned dentin. In addition to being nontoxic, the inhibition of rhMMP-2 by SCMC was confirmed to be dose-dependent. Significance From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.
研究领域:
牙科材料学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Odontology
·
2025
·
IF 2.4
Kim Soyeon,Larnani Sri,Son Ji Eun,Lappanakokiat Napas,Truong Van Mai,Jin Bo-Hyoung,Park Young-Seok
This study investigates the effects of different treatment sequences involving demineralization, brushing, and remineralization on the calcium and phosphorus (Ca/P) content, surface roughness, and microhardness of dentin specimens. Bovine dentin samples were subjected to the following five treatment conditions: control, demineralization, demineralization followed by remineralization, demineralization followed by brushing then remineralization, and demineralization followed by remineralization then brushing. X-ray fluorescence spectroscopy was then utilized to assess the elementary composition changes, while scanning electron microscopy provided microstructural analyses. Surface roughness and microhardness were also quantified to assess the physical changes in dentin. The control group retained significantly higher Ca/P content compared with all treated groups, indicating that demineralization, regardless of subsequent treatment, leads to a substantial loss of hydroxyapatite. Among the treated groups, those that underwent remineralization immediately after demineralization manifested higher Ca/P retention compared with those that included brushing before remineralization. Additionally, microhardness measurements indicated that post-demineralization brushing negatively affected dentin’s microhardness. The sequence of demineralization, brushing, and remineralization treatments significantly affects Ca/P retention in dentin, along with its surface roughness and microhardness. Pre-remineralization brushing diminished mineral recovery, whereas exposure to mineral-rich beverage immediately after demineralization resulted in greater mineral deposition.
研究领域:
牙科医学
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材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Biomedical Materials
·
2014
·Vol. 9
·期 3
·035008
·
IF 2.922
Hongjie Huang,Xin Zhang,Xiaoqing Hu,Linghui Dai,Jingxian Zhu,Zhentao Man,Haifeng Chen,Chunyan Zhou,Yingfang Ao
Hydrogels are attractive for cartilage tissue engineering because of their high plasticity and similarity with the native cartilage matrix. However, one critical drawback of hydrogels for osteochondral repair is their inadequate mechanical strength. To address this limitation, we constructed a solid-supported thermogel comprising a chitosan hydrogel system and demineralized bone matrix. Scanning electron microscopy, the equilibrium scanning ratio, the biodegradation rate, biomechanical tests, biochemical assays, metabolic activity tests, immunostaining and cartilage-specific gene expression analysis were used to evaluate the solid-supported thermogel. Compared with pure hydrogel or demineralized matrix, the hybrid biomaterial showed superior porosity, equilibrium swelling and degradation rate. The hybrid scaffolds exhibited an increased mechanical strength: 75% and 30% higher compared with pure hydrogels and demineralized matrix, respectively. After three days culture, bone-derived mesenchymal stem cells (BMSCs) maintained viability above 90% in all three materials; however, the cell retention of the hybrid scaffolds was more efficient and uniform than the other materials. Matrix production and chondrogenic differentiation of BMSCs in the hybrid scaffolds were superior to its precursors, based on glycosaminoglycan quantification and hyaline cartilage marker expression after three weeks in culture. Its easy preparation, favourable biophysical properties and chondrogenic capacity indicated that this solid-supported thermogel could be an attractive biomaterial framework for cartilage tissue engineering.
研究领域:
生物材料学
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再生医学
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组织工程学
✓ 已验证
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2023
·Vol. 12
·期 19
·2300100
·
IF 10
Zihuai Zhou,Jiachen Li,Zhe Wang,Hongli Zhang,Yiru Wang,Dongni Shen,Zhifang Wu,Minjuan Shen,Haihua Pan,Qi Wang,Ruikang Tang,Matthias Hannig,Baiping Fu
Dentin hypersensitivity (DH) is triggered by external stimuli irking fluid flow through exposed dentinal tubules (DTs). Three commercially available desensitizing agents as control in this study only achieve limited occlusion depths of ≈10 µm in the DTs as well as scarce remineralization of demineralized dentin matrix. Herein, polyelectrolyte-calcium complexes pre-precursor (PCCP) process is proposed for managing DH that demineralized dentin with exposed DTs is rubbed with ultrahighly concentrated polyelectrolyte-calcium suspension (4 g L −1 -5.44 m ) followed by phosphate solution (3.25 m ), each 10 min, leading to heavy remineralization of demineralized dentin and compact occlusion of the DTs over 200 µm after 1 day of in vitro and in vivo incubation. For the first time, it is demonstrated that the PCCP process relies on the pH-dependent electrostatic attraction between electropositive polyelectrolyte-calcium complexes and electronegative inwalls of DTs comprised of collagen fibrils and hydroxyapatite crystals under alkaline condition. The PCCP process might shed light on a promising dentin desensitizing strategy for DH management via rapid in-depth DT occlusion and remineralization of demineralized dentin.
研究领域:
牙科材料
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牙科研究
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生物矿化
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jiachi Lei,Jun Zhang,Kuntai Li,Hongyu Qin,Hai Liu,Pei Li,Shucheng Liu,Jingliang Xu
Existing methods for chitin extraction usually produce substantial waste, which poses ecological hazards. Natural deep eutectic solvent (NADES) offers a promising one-step pretreatment alternative, replacing the resource-intensive demineralization (DM) and deproteinization (DP) process. Hence, in this study, the influence of various acidic NADES, on achieving a simplified one-step DM and DP in the chitin extraction process was investigated. The study yielded chitin with 87.73 % purity, and microstructural analysis showed that NADES pretreatment minimally affected chitin quality without deacetylation. In addition, chitin extracted using choline chloride-oxalic acid as a carrier displayed excellent performance in the immobilization of Geobacillus thermocatenulatus lipase 2 (GTL2) because of obvious Ca 2+ activation effect. This process contributed to enhancement of immobilized enzyme activity. The immobilized GTL2 showed excellent hydrolytic capabilities, with its highest activity reaching 547.80 ± 20.62 U/mg, significantly better than the five commercial lipases that exhibited <40 % of the enzyme activity. Furthermore, the hydrolytic capacity of immobilized GTL2 was notably high for 4-nitrophenyl butyrate, measuring 935.47 ± 51.60 U/mg. This study provided a constructive approach for the one-step pretreatment of shrimp shells with organic acid-based NADES to isolate and purify chitin and its potential application as an immobilized carrier to enhance enzyme activity.
研究领域:
生物技术
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酶工程
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材料科学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Macromolecular Science Part B-Physics
·
2024
·
IF 1.2
Jie Jiang,Jianjun Fang,Wenjuan Liu,Meimei Guo,Zhongjia Lu,Heng Tang,Yi Lan,Zhihua Zhou
Composite porous microcarriers based on demineralized bone extracellular matrix (DBECM) and chitosan (Cs) were prepared using a combined emulsion and freeze-drying method. The effects of digestion by pepsin on the DBECM properties were investigated. The morphologies and porous structure of the DBECM/Cs porous microcarriers were observed by using a scanning electron microscope (SEM). The cytocompatibility of the DBECM/Cs porous microcarriers were studied by using cell counting kit-8 (CCK-8) and calcein acetoxymethyl ester/propidium iodide (CAM/PI). DBECM was digested into small pieces with a loose porous sponge structure and the digested DBECM showed an amorphous state. The resultant DBECM/Cs porous microcarriers showed a spherical shape and an interconnective porous structure. The CCK-8 and CAM/PI analyses indicated that the DBECM/Cs porous microcarriers were non-cytotoxic and showed good biocompatibility, providing a more favorable growth environment for cells.
研究领域:
生物医学工程
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材料科学
·
组织工程
✓ 已验证
DOI
高影响因子
JOURNAL OF DENTISTRY
·
2025
·
IF 5.5
Bing Liang,Xiaolu Chen,Yi Sun,Xinni Pan,Ye Lin,Qi Gao,Yuedan Xu,Ling Zhang
Objectives The study investigated the biomimetic mineralization effect of a self-etch adhesive loaded with amorphous fluorinated calcium phosphate (AFCP) nanoparticles. Methods In this study, fluoride was applied to synthesize AFCP nanoparticles, which were characterized by high resolution transmission electron microscope, selected area electron diffraction and Fourier-transform infrared spectroscopy. Subsequently, the self-etch adhesive (Clearfil S3 Bond) was mixed throughly with 20 wt% of AFCP. The single-layer reconstituted collagen fibrils and demineralized dentin were used to investigate the mineralization effects of AFCP nanoparticles as well as Clearfil S3 Bond loaded with AFCP. Moreover, the Cell Counting Kit-8 assay was conducted to evaluate the cytotoxicity of AFCP-loaded adhesive. Results The AFCP nanoparticles were successfully synthesized and characterized as an amorphous phase, which demonstrated better effectiveness in collagen fibril mineralization compared to amorphous calcium phosphate nanoparticles. Both AFCP nanoparticles and adhesive loaded with AFCP induced intrafibrillar mineralization of single-layer collagen fibrils. The incorporation of AFCP nanoparticles into adhesive led to the formation of remineralized crystals within the demineralized dentin. Moreover, cytotoxicity tests confirmed the biocompatibility of the AFCP-loaded adhesive. Conclusions The incorporation of AFCP nanoparticles into the self-etch adhesive facilitated collagen fibril mineralization and remineralization of demineralized dentin. Clinical Significance Incorporating fluoride, a commonly used anti-caries element, into the self-etch adhesive in the form of AFCP nanoparticles enables its biomimetic mineralization in restorative treatments, presenting a potential approach for developing a novel adhesive system to prevent dental caries clinically.
研究领域:
牙科材料学
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生物医学工程
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口腔医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 30
·6378-6389
·
IF 5.344
Zhuo Liang,Yue Luo,Yonggang Lv
A demineralized bone matrix (DBM) scaffold has good biocompatibility, low antigenicity, a natural porous structure and no cytotoxicity, and so it is an appropriate material for bone regeneration. However, osteoinductive growth factors are often removed during preparation, which destroys the osteoinductive capacity of the DBM scaffold. Biomaterials combined with gene therapy is a promising approach to effectively avoid this adverse side effect. This study develops a human bone morphogenetic protein 2 (hBMP2) gene-activated DBM scaffold to enhance the osteoinductive capacity of DBM and improve bone repair. Bone marrow mesenchymal stem cell (MSC)-derived microvesicles (MVs) were obtained, and polyethyleneimine (PEI) and human bone morphogenetic protein 2 (hBMP2) plasmids (phBMP2) were sequentially coated on the MVs by layer-by-layer (LBL) self-assembly to form an MVs–PEI/phBMP2 non-viral gene vector. Finally, the gene-activated scaffold (DBM/MVs–PEI/phBMP2) was prepared by loading MVs–PEI/phBMP2 onto a DBM scaffold. The experimental results show that the MVs–PEI/phBMP2 exhibits higher transfection efficiency and lower cytotoxicity to MSCs when the MVs/PEI weight ratio = 5, and could enhance the osteogenic differentiation of MSCs in vitro. Subcutaneous implantation into rats showed that the DBM/MVs–PEI/phBMP2 scaffold could efficiently enhance the deposition of: collagen fibers, osteocalcin, osteopontin and CD34 endogenous proteins. Rabbit femoral condyle defect experiments proved that the DBM/MVs–PEI/phBMP2 scaffold could significantly promote bone repair. This study presents a novel, highly efficient and low cytotoxicity gene delivery vector based on MVs. The gene-activated DBM scaffold based on MVs not only could promote bone formation but also angiogenesis, implying that this kind of gene-activated scaffold is a promising bone substitute material.
研究领域:
组织工程
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再生医学
·
基因治疗
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生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Jiayan Ge,Yuting Wu,Yushan Han,Chengrong Qin,Shuangxi Nie,Shijie Liu,Shuangfei Wang,Shuangquan Yao
Effective removal of alkali and alkaline earth metals (AAEM) is of great significance for promoting biomass pyrolysis. In this study, demineralization via hydrothermal pretreatment was performed, and the effect on the pyrolysis behavior was evaluated by thermogravimetric analysis (TGA) and thermal pyrolysis–gas chromatography-mass spectrometry (Py–GC/MS). The effects of reaction temperature, time, and pH on the dissolution rate of K + , Ca 2+ , and Mg 2+ were investigated. The optimal total dissolution rate of the metal elements was 42.10%. Compared with acid leaching, hydrothermal pretreatment allowed a higher crystallinity index. It significantly changed the pyrolysis behavior. The relative content of sugar in pyrolysis products was as high as 58%. The chemical compound distribution was concentrated in the range between C6 and C9, which was conducive for the refinement of gasoline by upgrading. This means that hydrothermal pretreatment has efficient demineralization, which promoted the thermal degradation behavior of biomass.
研究领域:
生物质能源
·
化学工程
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）