CyTA-Journal of Food
·
2016
·
IF 0.769
Ying-Hua Zhang,Huan Wang,Yan-Le Liu,Xin-Huai Zhao
In this study, two horseradish peroxidase (HRP)-based modifications were used to treat whey protein isolate (WPI) and skim milk powder (SKMP) at protein concentration of 50 g/L, pH 7.0, and 37°C, via dityrosine formation in protein molecules. When HRP, glucose oxidase, and glucose were used to treat WPI and SKMP, suitable usages of HRP, glucose oxidase, and glucose, and reaction time were selected from single factor trials based on relative dityrosine contents of the modified products. After that, the selected HRP plus the calculated H2O2 were used to treat WPI and SKMP. Electrophoretic analysis demonstrated that the modified products contained some cross-linked proteins with greater molecular weights. Other analyses showed that the modified products had more ordered secondary structure, and higher in vitro α-lactalbumin and β-lactoglobulin antigenicity than the respective substrates. HRP–glucose oxidase–glucose system was more potent than HRP–H2O2 system to enhance in vitro antigenicity of WPI and SKMP.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF DAIRY SCIENCE
·
2022
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IF 4.225
Fan Yang,Xin Ma,Wei Hu,Ziyi Xiong,Meijiang Huang,Yong Wu,Xuanyi Meng,Zhihua Wu,Anshu Yang,Xin Li,Hongbing Chen
ABSTRACT Dairy processing can alter the digestion stability and bioavailability of cow milk proteins in the gastrointestinal tract. However, analysis of stable linear epitopes on cow milk allergens that could enter into intestinal mucosal is limited. Thus, this study aimed to investigate the digestion and transportation properties and residual allergen epitopes entering into gastrointestinal mucosa of 3 commercial dairy products, including pasteurized milk (PM), ultra-heat-treated milk (UHTM), and dried skim milk (DSM). In this work, the digestive stability of the 3 kinds of dairy products has been performed in a standard multistep static digestion model in vitro and characterized by Tricine-SDS-polyacrylamide gel electrophoresis and reversed-phase HPLC. With respect to gastrointestinal digestion in vitro, the main allergens including β-lactoglobulin (β-LG), α-lactalbumin (α-LA), and caseins were degraded gradually, and the resistance peptides remained in the PM with a molecular weight of range from 3.4 to 5.0 kDa. Simultaneously, the potential allergenicity of the cow milk proteins was diminished gradually and is basically consistent after 60 min of gastrointestinal digestion. After gastrointestinal digestion, the remaining peptides were transported via an Ussing chamber and identified by liquid chromatography-MS/MS. By alignment, 10 epitopes peptides were identified from 16 stable peptides, including 5 peptides (AA 92–100, 125–135, 125–138, and 149–162) in β-LG, 2 peptides in α-LA (AA 80–93 and 63–79), 2 peptides in α S1 -casein (AA 84–90 and 125–132), and 1 peptide (AA 25–32) in α S2 -casein were identified by dot-blotting mainly exist in UHTM and PM. This study demonstrates dairy processing can affect the digestion and transport characteristics of milk proteins and in turn alter epitope peptides release.
研究领域:
食品科学
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过敏原学
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蛋白质组学
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营养学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
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IF 7.514
Zhiqiang Tan,Qiang Chen,Yongguang Yin,Yanwanjing Liu,Yaohui Lin,Qingsheng Bai,Mengxin Wu,Weirong Yao,Shuxia Xu,Jingfu Liu
Zinc oxide nanoparticles (ZnO NPs) are recently recommended as food additives owing to their outstanding nutritive function. Therefore, understanding their comprehensive information and stability in food samples is highly necessitated. However, the characterization of ZnO NPs in the complex food matrices remains a great challenge, limiting an in-depth understanding of their transformation during food storage. In this study, the hollow fiber flow field-flow fractionation was combined with UV–Vis absorption spectroscopy and inductively coupled plasma optical emission spectroscopy to assess the dissolution behaviors of ZnO NPs in skimmed milk powder solutions by monitoring the changes in the residual ZnO NPs and the amount of dissolved Zn(II) ions. The simultaneous characterization of these two Zn species in skimmed milk powder solutions was achieved without the need for tedious sample pretreatments, and the dissolution of ZnO NPs in skimmed milk powder solutions had time- and temperature-dependent behaviors.
研究领域:
食品科学
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分析化学
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纳米技术
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2022
·Vol. 45
·期 15
·3054-3062
·
IF 3.614
Qin Lei,Abduh Murshed,Anthony Chukwunonso Ogbuehi,Qian Peng,Yiqing Zhang,Fenyong Sun,Qi Zhong,Lei Jin,Hao Wang
Phosphorylation is one of the most important protein post-translational modifications, which possesses dramatic regulatory effects on the function of proteins. In consideration of the low abundance and low stoichiometry of phosphorylation and non-specific signal suppression, efficient capture of the phosphoproteins from complex biological samples is critical to meet the need for protein profiling. In this work, a facile preparation of titanium (IV)-immobilized O-phospho-L-tyrosine modified magnetic nanoparticles was developed for the enrichment of intact phosphoproteins. The prepared magnetic nanoparticles were characterized by various instruments and had a spherical shape with an average diameter of 300 nm. The adsorption isotherms were investigated and the maximum capacity for β-casein was calculated to be 961.5 mg/g. Standard protein mixtures and biological samples (non-fat milk and human serum) were selected to test the enrichment performance. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis demonstrated the excellent enrichment performance with high selectivity. With the superparamagnetic property, titanium (IV)-immobilized O-phospho-L-tyrosine modified magnetic nanoparticles were convenient for the practical application and clinical promotion, thus having a promising prospect in the field of phosphoprotein research.
研究领域:
生物化学
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蛋白质组学
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纳米技术
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Wanqi Zhang,Zhicong Sun,Yuhang Tian,Yaoting Mou,Yemin Guo,Xia Sun,Falan Li
Streptomycin (STR) is an antibiotic fungicide that is easily enriched in the environment and threatens human health. In this paper, a ratiometric fluorescence aptasensor with silver nanoclusters-skimmed milk powder@zeolitic imidazolate framework-8 (AgNCs-SMP@ZIF-8) fluorescent material as the response signal and carbon quantum dots (CQDs) functionalized with aptamer as the reference signal was prepared. Magnetic graphene oxide selection technology was used to screen high-affinity STR-specific aptamer. The truncation strategy for removing non-binding site bases was based on molecular simulation docking, and it was found that the stem-loop structure formed by the truncated aptamer 52 (Apt52) was used for STR-specific recognition and had a higher STR affinity ( K d = 14.52 nM) compared to the initial 79-nucleotide aptamer. AgNCs was then covered in a protein coating and encapsulated in ZIF-8 to obtain AgNCs-SMP@ZIF-8 with enhanced red fluorescence intensity . The added Cu 2+ rapidly penetrated into ZIF-8 to induce red fluorescence quenching , while the CQDs modified by Apt52 maintained green fluorescence. Hence, the constructed ratiometric fluorescent sensor with Apt52 as the recognizing element can detect STR within 90 s with a detection limit of 0.98 nM. The ratiometric probe proposed has considerable potential for STR residue detection in milk.
研究领域:
分析化学
·
生物技术
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纳米技术
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Chen Jiakai,Wang Danni,Wang Bing,Zhou Chengwei,Ding Chuan-Fan,Yan Yinghua
In the present study, click chemistry and Schiff base reactions were simultaneously applied to prepare polymer brush (PEG)–functionalized MOF materials (UiO-66-NH 2 ) and immobilized with Ti 4+ (MOF-Brush-THBA-Ti 4+ ) for phosphopeptide analysis. The material has a detection limit of 0.5 fmol, a selectivity of 2000:1, and a loading capacity of 133 mg/g for phosphopeptides. It also demonstrated great repeatability (10 cycles) and recovery rate (96.7 ± 1.4%). During the analysis of bio-samples, 4 specific phosphopeptides were identified in endogenous breast cancer serum, while 11 phosphopeptides were identified in skimmed milk. Moreover, 47 phosphopeptides correlated with 29 phosphorylated proteins were selectively identified from normal control serum, and 66 phosphopeptides correlated with 26 phosphorylated proteins were identified from breast cancer serum. Further analysis of gene ontology (GO) revealed that the detected phosphorylated proteins associated with breast cancer included positive regulation of receptor-mediated endocytosis, proteolysis, extracellular exosome, heparin binding, and chaperone binding. These findings suggest that these associated pathways might contribute to the etiology of breast cancer. Overall, this application exhibits enormous potential in the identification of phosphorylated peptides within bio-samples.
研究领域:
分析化学
·
生物化学
·
癌症生物学
·
蛋白质组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）