ACTA PHYSIOLOGIAE PLANTARUM
·
2014
·Vol. 36
·期 9
·2441-2451
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IF 1.524
Chen Jingxin,Mao Linchun,Mi Hongbo,Zhao Yuying,Ying Tiejin,Luo Zisheng
To study the detachment stress on the ripeness of strawberry fruit, physiological characteristics of strawberry fruit on and off plant during ripeness and senescence processes were investigated. The results indicated that the ripeness of strawberry fruit upon detachment was accelerated, in terms of firmness, soluble solid content and especially color development. The color of fruit off plant changed rapidly from white to full red in 1–2 days. The respiratory rate in fruit off plant was strengthened, higher than that on plant. Abscisic acid level and ethylene production in fruit off plant were also higher than those on plant and auxin degradation was exacerbated by detachment. Expression levels of FaMYB1 , FabHLH3 and FaTTG1 were generally reduced with phenotypes of redder color and more anthocyanin accumulation in fruit off plant. Results also suggested that the detachment initially stimulated ethylene and abscisic acid production and auxin degradation, which modulated ripening-related gene expression and at last enhanced fruit pigmentation.
研究领域:
植物学
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生理学
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产品关联
DOI
高影响因子
Horticulturae
·
2023
·Vol. 9
·期 7
·789
·
IF 3.1
Tao Xu,Min Zhang,Tianchi Chen,Lili Gong,Lingling Hu,Jie Yang,Haoxuan Si,Yueyan Wu
Suboptimal light stress in grapevines is increasing worldwide with the spread of grapevine cultivation, which can affect grapevine physiology and productivity, such as in Southern China. Abscisic acid (ABA) is an important hormone in plant adaptive responses to abiotic stress, including low light stress. However, ABA signaling pathway genes (APGs) are not well characterized in the grapevine, and little is known of their potentially mitigating role in grapevine growth under weak light stress. Our study aimed to explore the potential role of the ABA signaling pathway in the response of grapevines to suboptimal light conditions. In this study, APGs were identified in the grapevine genome, and the distribution of conserved motifs was shown to reflect their phylogenetic relationships. Gene duplication analysis indicated that segmental duplication was an important driver for gene expansion in the grapevine ABA signaling pathway. Suboptimal conditions of light were shown to seriously affect the growth of grapevine leaves and berries, with the differential regulation of APGs in the grapevine. Our study summarizes the basic characteristics of APGs in grapevine, which can now be examined further for their roles in grapevine’s response to suboptimal light conditions.Keywords:grape;weak light stress;abscisic acid;gene family
研究领域:
植物科学
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分子生物学
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胁迫生理学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2021
·Vol. 23
·期 1
·101
·
IF 5.924
Jianfa Cai,Xuelian Mo,Chenjin Wen,Zhen Gao,Xu Chen,Cheng Xue
Strawberry is a soft fruit with short postharvest life, due to a rapid loss of firmness. Pectin methylesterase (PME)-mediated cell wall remodeling is important to determine fruit firmness and softening. Previously, we have verified the essential role ofFvPME38in regulation of PME-mediated strawberry fruit softening. However, the regulatory network involved in PME-mediated fruit softening is still largely unknown. Here, we identified an R2R3-type MYB transcription factor FvMYB79, which activates the expression level ofFvPME38, thereby accelerating fruit softening. During fruit development,FvMYB79co-expressed withFvPME38, and this co-expression pattern was opposite to the change of fruit firmness in the fruit of ‘Ruegen’ which significantly decreased during fruit developmental stages and suddenly became very low after the color turning stage. Via transient transformation, FvMYB79 could significantly increase the transcriptional level ofFvPME38, leading to a decrease of firmness and acceleration of fruit ripening. In addition, silencing ofFvMYB79showed an insensitivity to ABA-induced fruit ripening, suggesting a possible involvement ofFvMYB79in the ABA-dependent fruit softening process. Our findings suggest FvMYB79 acts as a novel regulator during strawberry ripening via transcriptional activation ofFvPME38, which provides a novel mechanism for improvement of strawberry fruit firmness.Keywords:fruit softening;strawberry;FvMYB79;FvPME38
研究领域:
植物生物学
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分子遗传学
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农学
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DOI
PubMed
高影响因子
PLoS One
·
2022
·Vol. 17
·期 2
·e0263530
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IF 3.752
Shunde Su,Tengfei Zhu,Jun Su,Jian Li,Qing Zhao,Xiangyang Kang,Renhua Zheng
Flooding is one of the main abiotic stresses suffered by plants. Plants respond to flooding stress through regulating their morphological structure, endogenous hormone biosynthesis, and genetic signaling transduction. We previously found that Fokienia hodginsii varieties originating from Gutian exhibited typical flooding tolerance traits compared to three other provenances (Yongzhou, Sanming, Nanping), expressed as increased height, longer diameter at breast height (DBH), and smaller branch angle. Herein, the changes in endogenous gibberellins (GA) and abscisic acid (ABA) contents were measured under flooding stress in F . hodginsii , and ABA was found to decrease, whereas GA increased with time. Furthermore, the GA and ABA contents of the varieties originating from Gutian and the three other provenances were measured, and the results indicated that F . hodginsii from Gutian could respond more rapidly to flooding stress. The transcriptomes of the varieties originating from Gutian and the other three provenances were compared using RNA sequencing to explore the underlying genetic mechanisms of the flood-resistant phenotypes in F . hodginsii . The results indicated that two flood-stress response genes (TRINITY_DN142_c0_g2 and TRINITY_DN7657_c0_g1) were highly related to both the ABA and GA response in F . hodginsii .
研究领域:
植物学
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分子生物学
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遗传学
✓ 已验证
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
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2016
·
IF 2.618
Xiaoya Tao,Linchun Mao,Jiayin Li,Jingxin Chen,Wenjing Lu,Si Huang
The development of wound periderm is vital to prevent water vapor loss and pathogen invasion in wounded fruit. The effect of abscisic acid (ABA) on wound-healing in harvested tomato fruit was evaluated in this study. Wounded tomato fruit were treated with ABA or fluridone (FLD, an inhibitor of ABA biosynthesis), respectively, and allowed to wound-heal at 20 °C in darkness for 4 days. Weight loss of tomato fruit reflected the intuitionistic effects of different treatments during wound-healing. Autofluorescence and histochemical staining demonstrated the development of suberized wound periderm in harvested tomato fruit at 3–4 day after wounding. Alkanoic acids, n -alkanes, unsaturated fatty acids and pentacosane represented the major components in the suberin. ABA-treated fruit showed enhanced autofluorescence, histochemical intensity and suberin deposition along with increased activities of phenylalanine ammonia-lyase (PAL) and peroxidase (POD). FLD treatment, however, alleviated the features of suberization and enzyme activities during wound-healing. The results suggest that ABA is involved in the stimulation of wound-induced suberization in harvested tomato fruit.
研究领域:
植物学
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园艺学
✓ 已验证
DOI
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
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2021
·
IF 4.401
Xuehua Liu,Lijun Wang,Songcui Wu,Lu Zhou,Shan Gao,Xiujun Xie,Lepu Wang,Wenhui Gu,Guangce Wang
The formation of resting cells/spores in marine diatoms helps them to survive in environmental changes, whose sedimentation characteristics also drive enhanced carbon fluxes export to the deep ocean. However, the molecular mechanism underlying the transformation remains unclear. Based on the upregulation of ferritin during dormancy in microorganism and its' crucial role in coping with environmental stress, we constructed the ferritin overexpression and silence strains in model marine diatom, Phaeodactylum tricornutum (FTN-OE and FTN Si, respectively). The morphological transformation from fusiform to ovoid were observed among the FTN-OE strains but not the wide type and FTN-Si strains. These FTN-OE oval cells exhibited typical characteristics of resting cells and benthic adaptation, such as lower growth rates, low-light adaptation, silicified valves, the excretion of exopolymeric substances, upregulation of stress-resistant proteins and increased nitrate reserves. Furthermore, FTN-OE oval cells showed increased abscisic acid synthesis and signaling pathways in the transcriptome and proteome . And the abscisic acid signal was detected in FTN-OE oval cells exclusively (2.96 ng g −1 FW), but absent in the FTN Si, FTN-OE fusiform or WT cells. Overall, our results demonstrate that ferritin overexpression in marine diatom have a close relationship with morphological transformation from fusiform to ovoid morphotype , after which they enter a resting stage, which is accompanied by abscisic acid accumulation. This improves our understanding of the molecular processes in diatom resting cells/spores' formation and suggests that ferritin accumulation might improve the sequestration of photosynthetically fixed atmospheric CO 2 into the deep sea.
研究领域:
海洋生物学
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分子生物学
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产品关联
DOI
高影响因子
Toxics
·
2024
·Vol. 12
·期 8
·577
·
IF 3.9
Yi Kang,Cheng-Zhi Li,Abid Ullah,Qing Zhang,Xiao-Zhang Yu
Potential toxic element (PTE) pollution has emerged as a significant environmental and social concern in global agriculture. Chromium (Cr) occurs in different oxidation states naturally, among them Cr(VI), which is highly toxic. This study carried out biochemical and molecular tests to elucidate the accumulation of total soluble phenolics (TSPs) in rice plants exposed to Cr(VI) at 2.0, 8.0, and 16.0 mg Cr/L, emphasizing the interaction between polyamines (PAs) and abscisic acid (ABA). The results revealed significant Cr accumulation in different tissues of rice plants, which hindered their growth. Cr(VI) exposure increased the ABA concentration, with higher levels detected in the shoots than in the roots. The TSP concentration in rice tissues showed a positive relationship with the supplied concentrations of Cr(VI). The measured PAs, including spermine (Spm), putrescine (Put), and spermidine (Spd), exhibited varied responses to Cr(VI) stress, with only Spm concentration increasing with Cr(VI) concentrations. Real-time qRT-PCR showed PAs and ABA synthesis-associated genes such asOsADC1,OsAIH,OsCPA1, andOsCPA4were significantly up-regulated in shoot of rice plants treated with Cr(VI). These genes are associated with the second pathway of Put synthesis, originating from Arg. Almost all genes activated in the Met pathway were significantly up-regulated as well. Moreover, the genes involved in the interconversion among the three species of PAs exhibited completely different responses to Cr(VI) exposure. Overall, the biochemical analysis and gene expression data indicate that the interaction between ABA and Spm is likely to enhance the TSP levels in rice plants subjected to Cr(VI) toxicity.
研究领域:
植物学
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环境科学
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DOI
PubMed
高影响因子
PLANTA
·
2015
·Vol. 243
·期 1
·183-197
·
IF 3.263
Chen Jingxin,Mao Linchun,Lu Wenjing,Ying Tiejin,Luo Zisheng
Main conclusion
Auxin and abscisic acid regulate strawberry fruit ripening and senescence through cross-talk of their signal transduction pathways that further modulate the structural genes related to physico-chemical properties of fruit.
The physiological and transcriptomic changes in harvested strawberry fruits in responses to IAA, ABA and their combination were analyzed. Exogenous IAA delayed the ripening process of strawberries after harvest while ABA promoted the postharvest ripening. However, treatment with a combination of IAA and ABA did not slow down nor accelerate the postharvest ripening in the strawberry fruits. At the molecular level, exogenous IAA up regulated the expressions of genes related to IAA signaling, including AUX/IAA , ARF , TOPLESS and genes encoding E3 ubiquitin protein ligase and annexin, and down regulated genes related to pectin depolymerization, cell wall degradation, sucrose and anthocyanin biosyntheses. In contrast, exogenous ABA induced genes related to fruit softening, and genes involved in signaling pathways including SKP1 , HSPs , CK2 , and SRG1 . Comparison of transcriptomes in responses to individual treatments with IAA or ABA or the combination revealed that there were cooperative and antagonistic actions between IAA and ABA in fruit. However, 17 % of the differentially expressed unigenes in response to the combination of IAA and ABA were unique and were not found in those unigenes responding to either IAA or ABA alone. The analyses also found that receptor-like kinases and ubiquitin ligases responded to both IAA and ABA, which seemed to play a pivotal role in both hormones’ signaling pathways and thus might be the cross-talk points of both hormones.
研究领域:
植物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
BMC PLANT BIOLOGY
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2022
·Vol. 22
·期 1
·1-15
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IF 5.26
Wang Haitang,Zhou Xuesong,Liu Chuchu,Li Weixi,Guo Wangzhen
Background In eudicots, germination begins with water uptake by the quiescent dry seed and is greatly related to the permeability of micropyle enriched callose layers. Once imbibition starts, seeds undergo a cascade of physiological, biochemical, and molecular events to initiate cellular activities. However, the effects of callose on water uptake and following seed metabolic events during germination are largely unknown. Cotton ( Gossypium hirsutum ) is a eudicot plant with natural fiber and edible oil production for humans. Here, we addressed this question by examining the role of GhGLU19 , a gene encoding β-1,3-glucanase, in cotton seed germination. Results GhGLU19 belongs to subfamily B and was expressed predominately in imbibed seeds and early seedlings. Compared to wild type, GhGLU19 -suppressing and GhGLU19 -overexpressing transgenic cotton lines showed the higher and lower seed germination percentage, respectively. Callose was enriched more at inner integument (ii) than that in embryo and seed coat in cotton seeds. In GhGLU19 -suppressing lines, callose at ii of cotton seeds was greatly increased and brought about a prolonged water uptake process during imbibition. Both proteomic and transcriptomic analysis revealed that contrary to GhGLU19 -overexpressing lines, the glycolysis and pyruvate metabolism was decreased, and abscisic acid (ABA) biosynthesis related genes were downregulated in imbibed seeds of GhGLU19 -suppressing lines. Also, endogenous ABA was significantly decreased in GhGLU19 -suppressing line while increased in GhGLU19 -overexpressing line. Conclusions Our results demonstrate that suppression of GhGLU19 improves cotton seed germination via accumulating callose of inner integument, modulating glycolysis and pyruvate metabolism, and decreasing ABA biosynthesis. This study provides a potential way for improving germination percentage in cotton seed production, and other eudicot crops.
研究领域:
植物学
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遗传学
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分子生物学
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DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2017
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IF 1.624
Zhifei Chen,Zhi Wang,Yungui Yang,Min Li,Bingcheng Xu
Tall fescue ( Festuca arundinacea Schreb.) is a widely used cool-season turfgrass, and its growth is mainly limited by water deficit. Abscisic acid (ABA) and brassinolide (BR) are two important stress hormones regulating plant physiological processes and growth under water deficit. To investigate effects of exogenous ABA and BR on physiological and photosynthetic performance of tall fescue under water stress conditions, ABA (10 and 20 mg L −1 ) and BR (0.4 and 0.8 mg L −1 ) were applied individually and in combination (0.4 mg L −1 BR and 10 mg L −1 ABA) under three soil water regimes [75 ± 5% FC (HW), 50 ± 5% FC (MW) and 25 ± 5% FC (LW)] in the greenhouse. Results revealed that ABA and BR application markedly decreased the relative conductivity and malondialdehyde, notably increased leaf relative water content, antioxidant enzyme activity and proline content under water stress. ABA plus BR application was equally effective in improving the activities of antioxidant enzyme as ABA at 20 mg L −1 . ABA application reduced stomatal conductance and decreased both transpiration and net photosynthetic rate ( P n ), while BR application significantly increased P n and water use efficiency (WUE) by enhancing chlorophyll content. The ABA and BR combination application showed higher P n and WUE as well as BR single application. It indicated that ABA and BR combination application increased photosynthetic capacity, and reduced the effect on photosynthetic loss caused by ABA under water stress. All these confirmed that ABA plus BR application exhibited a synergistic interaction on enhancing drought tolerance and photosynthesis of tall fescue under water stress.
研究领域:
植物生理学
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园艺学
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产品关联
DOI
高影响因子
Antioxidants
·
2025
·Vol. 14
·期 5
·576
·
IF 6.6
Jiaming Chen,Yuting Fu,Shaoping Weng,Jianguo He,Chuanfu Dong
Scale drop disease virus (SDDV) poses an escalating threat to global aquaculture, prompting an urgent need for research. Our study found that SDDV infection upregulates genes related to iron, oxidative stress, and lipid metabolism, causing iron overload, reactive oxygen species (ROS) accumulation, and ultimately ferroptosis. Among the tested antioxidants, vitamin C (VC) demonstrated the most potent inhibitory effect in mandarin fish, reducing SDDV-induced mortality by 37.5%. qPCR and IFA results showed that VC effectively suppressed SDDV infection; decreased ROS, lipid peroxidation (LPO), and iron levels; and enhanced glutathione peroxidase 4 (GPX4) expression in infected cells. Mechanistically, VC’s inhibitory effect was reversed by the nuclear factor erythroid 2-related factor 2 (Nrf2) inhibitor ML-385, indicating an Nrf2-dependent pathway. VC promoted Nrf2 nuclear translocation and activated downstream antioxidant genes. Moreover, VC modulated inflammation by regulating pro- and anti-inflammatory factors. These findings suggest VC as a promising therapeutic for SDDV infection.
研究领域:
水产养殖学
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病毒学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Mengjun Yun,Yu Xiong,Zhuobing Wang,Lianjie Xie,Hanwen Ye,Xiaofang Yuan,Weiyi He,Binqing Chen,Zhanjun Lu,Wei Chen
Plants perceive proteins from insect-derived oral secretion (OS) and regulate the classical endogenous hormone jasmonic acid to resist insects, but the role of abscisic acid (ABA) in this process is poorly understood. In this study, we used the specialist herbivorous caterpillar Plutella xylostella and cruciferous plants as a model to investigate how the ABA hormone responds to the OS and its core peptide from the insect. Through proteomics and Western blotting analysis, glucosinolate sulfatase 1 (GSS1) was identified in OS. Yeast library screening revealed that GSS1 and its 28-amino-acid core peptide (GSS1-P1) interact with ABA biosynthetic enzyme ABA1. Arabidopsis overexpressing GSS1 and plants treated with synthetic GSS1-P1 showed elevated ABA levels. Transcriptome analysis and RT-qPCR confirmed that GSS1-P1 upregulates WRKY18 and ABA1 expression, modulating ABA production. Both GSS1-P1 application and optimal ABA concentrations enhanced plant resistance to herbivory. Our study shows that GSS1 and its peptide stimulate ABA production, boosting plant–insect resistance and highlighting ABA’s potential role in pest-stress response.
研究领域:
植物学
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分子生物学
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昆虫学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）