JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2015
·Vol. 96
·期 7
·2418-2425
·
IF 1.714
Xin-Tong Yao,Xin-Huai Zhao
BACKGROUND Transglutaminase (TGase) induces protein glycation and cross-linking, but results in lower solubility and digestibility due to excessive cross-linking. Deamidation of soy protein isolate (SPI) by HCl converts glutamine residues, and provides less opportunity for the two reactions. Two deamidated SPI products (DSPI1 and DSPI2) were thus glucosamine-glycated and cross-linked, to clarify the effects of pre-deamidation on the two reactions and properties of the products. RESULTS DSPI1 and DSPI2 had respective degrees of deamidation of 12.2% and 27.4%. They and SPI were used to generate three glycated and cross-linked products (GC-DSPI1, GC-DSPI2 and GC-SPI) containing glucosamine of 12.0, 4.4 and 19.7 g kg −1 protein, respectively, which were reflected in their infrared spectra at two regions. These three (especially GC-SPI) had higher water-binding than SPI (8.2–12.6 versus 6.2 g g −1 protein). GC-DSPI1 and GC-DSPI2 showed better enzymatic digestion than GC-SPI. Thermogravimetric and circular dichroism analyses verified that GC-DSPI1 and GC-DSPI2 had maximum degradation rates at temperatures 12–14 °C lower than GC-SPI, and possessed a more open secondary structure. CONCLUSION SPI deamidation decreases forthcoming glycation and cross-linking, and gives the products higher digestibility, less increased hydration, lower thermal stability, and a more open secondary structure. Pre-deamidation is applicable to control the properties of GC-proteins. © 2015 Society of Chemical Industry
研究领域:
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2023
·
IF 8.4
Wen-yu Zheng,Xin-xin Pang,Yue-tong Huang,Murtaza Ali,Sui-lin Qiu,Min Gu,Jia-ling Shen,Qiu-ya Lin,Si-xia Zheng,Xin-An Zeng,Lan Liao
The utilization of natural deep eutectic solvents (NADESs) for super-deamidation of wheat gluten represents a cost-effective approach to expand its potential applications as a plant protein source and reduce the occurrence of allergens. However, NADESs are susceptible to deconstruction during hydration, leading to unstable super-deamidation. Herein, we prepared a ternary hydrated NADES (HB3C1G1-N) composed of betaine, citric acid, and glucose, doubling the protein preparation concentration (50 mg/mL) compared to previous work and achieving a record-breaking degree of super-deamidation at 99.58%. Experimental and computational analyses revealed that the betaine in the NADES autonomously interacts with the citric acid and glucose at the hydrogen bond acceptor sites during hydration, reversing the disassembly of the hydrogen bond network in water and playing a crucial role in the accessibility of the buried amide group. The super-deamidated wheat gluten exhibited structural characteristics, such as fewer disulfide bonds and more low-molecular-weight subunits, resulting in a lightning-like microstructure in the model emulsion and improving the stability of oil droplets. This work demonstrates a green and scalable super-deamidation pathway and provides a foundation for broadening the application of super-deamidated wheat gluten.
研究领域:
食品科学
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分子生物学
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产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Wen-yu Zheng,Xiao-mei Wu,Ming-xuan Li,Sui-lin Qiu,Tu-di Yang,Rong Yang,Zhan-peng Chen,Shao-yun Wang,Lan Liao
The synergistic coupled improvement of functional properties by natural deep eutectic solvent (NADES) super-deamidated wheat gluten (WG) was investigated. Screening by eight NADESs, glucose-citric acid NADES (G-C, molar ratio at 2:1) super-deamidated WG (SDWG-N) exhibited the exceeding emulsification properties (22.37 m 2 /g) and foaming stability (85.61%), which were significantly higher than those of citric acid deamidated WG (CDWG). Degree of deamidation and protein recovery of SDWG-N (26 mg/mL) reached 95.47% and 96.77% respectively at 121 °C for 14 min at the condition that G-C was diluted to 18% (v/v). Supra-molecular structural properties of G-C NADES reflected that the charge inhomogeneity of G-C promoted the affinity of water to the amide groups via ionizing more H + and formed hydrogen bonds with amino acid residues, thus achieving the strong coupling super-deamidation of WG. SDWG-N showed higher stability as function of pH 2–12 than CDWG. Fluorescence and FTIR spectra explored more unfolded and flexible with greater exposed hydrophobic groups and lower degree of aggregation of SDWG-N, which exhibited the synergistic coupled effect of super-deamidation by G-C on WG. It suggested that SDWG-N would be used as a strong stabilizer at water/oil and gas/water interface with further potential to cooperate with other macromolecules for food use.
研究领域:
食品科学
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生物化学
✓ 已验证
DOI
高影响因子
CyTA-Journal of Food
·
2015
·
IF 0.824
Xin-Tong Yao,Xin-Huai Zhao
Caseinate was treated by diluted HCl solution to achieve degrees of deamidation of 16.6% and 28.1%, which resulted in lower surface hydrophobicity. Two deamidated caseinates were treated by transglutaminase and glucosamine to carry out glucosamine conjugation and cross-linking, to generate two modified caseinates (glucosamine-conjugated and cross-linked deamidated caseinates). Two modified caseinates contained less glucosamine (6.9–9.8 versus 12.6 g/kg protein) and higher reactable –NH2 (0.54–0.56 versus 0.51 mol/kg protein) than the glucosamine-conjugated and cross-linked caseinate, declaring they were less glycated and less cross-linked than caseinate. Two modified caseinates had better solubility in pH 3.0–9.0, higher water- and oil-binding and emulsion stability but lower emulsifying activity than caseinate; however, they also exhibited better solubility and emulsifying activity than the counterpart. It is thus concluded that HCl-induced deamidation was powerful enough to control transglutaminase-induced glucosamine conjugation and cross-linking as well as functional properties of the treated caseinates.
研究领域:
食品科学
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生物化学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Mengyuan Zhang,Luzhen Chen,Xuanbo Liu,Zhenyu Yu,Yibin Zhou,Yongquan Wang
This study aimed to elucidate the pathways through which covalent and non-covalent interactions between deamidated gliadin (DG) and tannic acid (TA) on influence the stability of Pickering emulsions. The interactions induced protein unfolding, as evidenced by increased ultraviolet absorption and a red shift in fluorescence emission. DG-TA composite nanoparticles effectively stabilized high internal phase emulsions, whereas DG nanoparticles alone did not. Covalent DG-TA nanoparticle stabilized Pickering emulsions (C-DGTAE) retained a consistent mean droplet size after 30 d of storage. Lipid hydroperoxide and malondialdehyde levels in C-DGTAE and N-DGTAE were reduced by 59.1 %–69.5 % and 38.9 %–44.4 %, respectively. Furthermore, the retention of β-carotene in the emulsions was significantly enhanced. All emulsions exhibited elastic behavior, characterized by higher G′ than G″. Notably, N-DGTAE demonstrated the highest apparent viscosity, G′ and G″, attributed to the connected nanoparticles around the droplets. Confocal laser scanning microscopy revealed that C-DGTAE droplets possessed the thickest layer, corroborated by the highest interfacial nanoparticle content of 76 % and an interfacial thickness of 441 nm. These findings suggest that covalent interactions enhance the interfacial nanoparticle layer, while non-covalent interactions promote nanoparticle networking, providing valuable insights for optimizing the stability of Pickering emulsions.
研究领域:
食品科学
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界面化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Lin Zhang,Luzhen Chen,Shiyi Li,Zhenyu Yu,Yibin Zhou,Yongquan Wang
This study aimed to improve the mechanical properties of wheat starch gels (WSG) and the stability and bioaccessibility of resveratrol (Res) in prolamin nanoparticles. Res-loaded gliadin (Gli), zein, deamidated gliadin (DG) and deamidated zein (DZ) nanoparticles were filled in WSG. The hardness, G′ and G′′ of WSG were notably increased. It can be attributed to the more ordered and stable structure induced by the interaction of prolamin nanoparticles and starch. The Res retention of nanoparticles and nanoparticle-filled starch gels was at least 24.6 % and 36.0 % higher than free Res upon heating. When exposed to ultraviolet, the Res retention was enhanced by over 6.1 % and 37.5 %. The in-vitro digestion demonstrated that the Res releasing percentage for nanoparticle-filled starch gels was 25.8 %–38.7 % lower than nanoparticles in the simulated stomach, and more Res was released in the simulated intestine. This resulted in a higher bioaccessibility of 82.1 %–93.2 %. The bioaccessibility of Res in Gli/Res/WSG and DG/Res/WSG was greater than that of Zein/Res/WSG and DZ/Res/WSG. More hydrophobic interactions occurred between Res and Gli, DG. The interactions between Res and zein, DZ were mainly hydrogen bonding. The microstructure showed that nanoparticles exhibited dense spherical structures and were uniformly embedded in the pores of starch gels.
研究领域:
食品科学
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纳米技术
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药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·
IF 4.1
Pimiao Huang,Wenke Zhao,Lei Cai,Ying Liu,Jing Wu,Chun Cui
BACKGROUND Enzymatic modification is an effective means of improving the functional properties, digestive properties, and in vitro digestion product physiological activity of proteins, thus significantly expanding protein uses in various food applications. RESULTS In this study, the addition of chymotrypsin (CT) at pH 9.0 and 11.0 was found to significantly improve the functional properties (solubility, foaming properties, water holding capacity, oil holding capacity, etc.) and digestive properties of extruded corn gluten meal (ECGM). Similar changes were observed when treating ECGM with glutaminase, protein glutaminase, and papain. These changes were likely due to the increase in number of carboxyl groups and the multiple effects of change in protein net charge and conformation caused by enzymatic deamidation. Of note, ECGM deamidated by CT showed the highest degree of deamidation, solubility, and gastrointestinal digestibility at pH 11.0, up to 44.92%, 43.75%, and 82.22%, respectively. In addition, CT-ECGM digestion product exhibited strong antioxidant activity and potential to promote alcohol metabolism in both a static digestion model and dynamic digestion model, even comparable to commercial corn peptides (CCP), while being inexpensive and of low bitterness compared to CCP. Meanwhile, the physiological activity enhanced as the molecular weight of digestion product decreased with the digested component having strongest activity. CONCLUSION This study may promote the application of ECGM as a food component in the food industry or even as a substitute for CCP. © 2023 Society of Chemical Industry.
研究领域:
食品科学
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酶学
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营养学
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DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2023
·Vol. 107
·期 10
·3217-3227
·
IF 5
Wang Yang,Chen Huanhuan,Zhao Meiqi,Feng Lei,Liu Zexin,Zeng Qiongya,Shi Wenqiang,Zhu Wen,Song Luyao,Zhu Jianwei,Lu Huili
Abstract Being an important immune stimulant of T lymphocytes and NK cells, the recombinant human interleukin-15 (rhIL-15) has been extensively researched in tumor immunotherapy or as a vaccine adjuvant. However, the rhIL-15 manufacturing level lags far behind its growing clinical demand due to the lack of efficient and exact analysis methodologies to characterize the trace by-products, typically redox and deamidation. In order to improve the production and quality control of rhIL-15, here we developed an expanded resolution reverse-phase high-performance liquid chromatography (ExRP-HPLC) approach to quickly and accurately analyze the oxidation and reduction by-products of rhIL-15, which may appear during the purification processes. Firstly, we developed RP-HPLC methods which can separate rhIL-15 fractions with different levels of oxidization or reduction, respectively, and the redox status of each peak was then determined by measuring the intact mass with a high-resolution mass spectrometer (UPLC-MS). To further clarify the complex pattern of oxidization of specific residues, the peaks with various oxidation levels were digested into pieces for peptide mapping to pinpoint the exact changes of oxygen and hydrogen atoms in the rhIL-15 by-products. In addition, we performed the ExRP-HPLC and UPLC-MS analysis of partially deamidated rhIL-15 to characterize their oxidation and reduction. Our work is the first in-depth characterization of the redox by-products of rhIL-15, even for deamidated impurities. The ExRP-HPLC method we reported can facilitate the rapid and accurate quality analysis of rhIL-15, which is substantially helpful for streamlining the industrial manufacturing of rhIL-15 to better meet the demands of clinical applications. Keypoints • The oxidization and reduction rhIL-15 by-products were characterized for the first time.
• The changes of oxygen and hydrogen atoms in rhIL-15 redox by-products were accurately determined by UPLC-MS.
• Oxidation and reduction by-products of deamidated rhIL-15 were further analyzed.
研究领域:
生物化学
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药学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Pharmaceutical Analysis
·
2024
·
IF 6.1
Chengyi Zuo,Jingwei Zhou,Sumin Bian,Qing Zhang,Yutian Lei,Yuan Shen,Zhiwei Chen,Peijun Ye,Leying Shi,Mao Mu,Jia-Huan Qu,Zhengjin Jiang,Qiqin Wang
Dynamic tracking analysis of monoclonal antibodies (mAbs) biotransformation in vivo is crucial, as certain modifications could inactivate the protein and reduce drug efficacy. However, a particular challenge (i.e. immune recognition deficiencies) in biotransformation studies may arise when modifications occur at the paratope recognized by the antigen. To address this limitation, a multi-epitope affinity technology utilizing the MOF@Au@peptide@aptamer composite material was proposed and developed by simultaneously immobilizing complementarity determining region (CDR) mimotope peptide (HH24) and non-CDR mimotope aptamer (CH1S-6T) onto the surface of MOF@Au nanocomposite. Comparative studies demonstrated that MOF@Au@peptide@aptamer exhibited significantly enhanced enrichment capabilities for trastuzumab variants in comparison to mono-epitope affinity technology. Moreover, the higher deamidation ratio for LC-Asn-30 and isomerization ratio for HC-Asn-55 can only be monitored by the novel bioanalytical platform based on MOF@Au@peptide@aptamer and LC-QTOF-MS. Therefore, multi-epitope affinity technology could effectively overcome the biases of traditional affinity materials for key sites modification analysis of mAb. Particularly, the novel bioanalytical platform can be successfully used for the tracking analysis of trastuzumab modifications in different biological fluids. Compared to the spiked PB model, faster modification trends were monitored in the spiked serum and patients’ sera due to the catalytic effect of plasma proteins and relevant proteases. Differences in peptide modification levels of trastuzumab in patients’ sera were also monitored. In summary, the novel bioanalytical platform based on the multi-epitope affinity technology holds great potentials for in vivo biotransformation analysis of mAb, contributing to improved understanding and paving the way for future research and clinical applications.
研究领域:
药学
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分析化学
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生物技术
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Pharmaceutica Sinica B
·
2023
·
IF 14.5
Jiawen Yang,Aixuan Zhou,Minyi Li,Qiaoxian He,Jingwei Zhou,Jacques Crommen,Wentao Wang,Zhengjin Jiang,Qiqin Wang
Due to low immobilized ligand density, limited binding capacity, and severe interference from serum proteins, developing ideal peptide-based biomaterials for precise recognition and in vivo analysis of biopharmaceuticals remains a huge challenge. In this study, mimotope peptide modified pompon mum-like biomimetic magnetic microparticles (MMPs, 3.8 μm) that mimic the specific functionalities of CD20 on malignant B cells were developed for the first time. Benefit from the numerous ligand binding sites (Ni 2+ ) on the pompon mum-like MMPs, these novel materials achieved ≥10 times higher peptide ligand densities (>2300 mg/g) and antibody binding capacities (1380 mg/g) compared to previous reported biomaterials. Leveraging the high specificity of the mimotope peptide, rituximab can be precisely recognized and enriched from cell culture media or serum samples. We also established an LC‒MS/MS method using the MMPs for tracking rituximab biotransformation in patient serum. Intriguingly, deamidation of Asn55 and Asn33, as well as oxidation of Met81 and Met34 were observed at the key complementarity determining regions of rituximab, which could potentially influence antibody function and require careful monitoring. Overall, these versatile biomimetic MMPs demonstrate superior recognition and enrichment capabilities for target antibodies, offering interesting possibilities for biotransformation analysis of biopharmaceuticals in patient serum.
研究领域:
生物技术
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药学
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分析化学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 28
·期 48
·69314-69328
·
IF 4.223
Yuan
Taikang,Wang Xudong,Zhao Xiaochen,Liu Tingting,Zhang Hongmin,Lv Yongtao,Wang Lei
Natural bornite (NBo), a sulfide mineral of copper and iron, is one of the main mineral raw materials for copper extraction. In this study, NBo-activated hydrogen peroxide (H 2 O 2 ) and persulfate processes (PS) for the degradation of minocycline (MNC) in aqueous solution were systemically investigated and compared. The MNC removal rates with the NBo/PS and NBo/H 2 O 2 processes were 86.40% and 87.50%, respectively. The mineralization rate of NBo/PS (33.96%) was higher than that of NBo/H 2 O 2 (29.94%) after reaction for 180 min. The effects of oxidant and activator dosage, pH, common inorganic anions (i.e., Cl − , NO 3 − , and HCO 3 − ), and water composition on MNC degradation were systematically evaluated. In addition, the degradation of MNC in natural water matrix and toxicity evaluation was also studied to better evaluate the feasibility of practical application of those two processes. The results of free radical quenching experiments and electron paramagnetic resonance spectroscopy (EPR) showed that HO· was the main activated species in the NBo/H 2 O 2 system, while SO 4 · − and HO· were the main activated species in the NBo/PS system. The degradation of MNC in the NBo/PS system was achieved through electron transfer, while the degradation of MNC in the NBo/H 2 O 2 system was mainly achieved through free radical addition. The degradation pathway mainly included deamidation reactions, C-C bond breakage and hydroxylation. Reusability of NBo showed that NBo was considerably stable in activating PS and H 2 O 2 for degradation of MNC, which was cost-effective activator. This work provides a new perspective on the degradation mechanism of pollutants by Fe-Cu bimetallic sulfide activation of PS and H 2 O 2 . Graphical abstract
研究领域:
环境科学
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化学工程
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水处理
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）