PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2018
·
IF 2.718
Chen Geng,Tengfei Zhao,Chunxian Yang,Qiaozhuo Zhang,Feng Bai,Junlan Zeng,Fangyuan Zhang,Xiaoqiang Liu,Xiaozhong Lan,Min Chen,Zhihua Liao
N -methylputrescine is the precursor of nicotine and pharmaceutical tropane alkaloids such as hyoscyamine . Putrescine N -methyltransferase (PMT) catalyzes the N -methylation of putrescine to form N -methylputrescine. While the role of PMT in nicotine biosynthesis is clear, knowledge of PMT in the biosynthesis of tropane alkaloids (TAs) and the regulation of polyamines remains limited. We characterized a PMT gene from Hyoscyamus niger , designated HnPMT that was specifically expressed in roots, especially in the secondary roots and dramatically induced by methyl jasmonate (MeJA). The GUS gene was specifically expressed in Arabidopsis roots or in the vascular tissues , including pericycles and endodermis , of the H. niger hairy root cultures , when it was driven by the 5′-flanking promoter region of HnPMT . The recombinant HnPMT was purified for enzymatic assays. HnPMT converted putrescine to form N -methylputrescine, as confirmed by LC-MS. The kinetics analysis revealed that HnPMT had high affinity with putrescine but low catalytic activity, suggesting that it was a rate-limiting enzyme . When HnPMT was suppressed in the H. niger plants by using the VIGS approach, the contents of N -methylputrescine and hyoscyamine were markedly decreased, but the contents of putrescine, spermidine and a mixture of spermine and thermospermine were significantly increased; this suggested that HnPMT was involved in the biosynthesis of tropane alkaloids and played a competent role in regulating the biosynthesis of polyamines . Functional identification of HnPMT facilitated the understanding of TA biosynthesis and thus implied that the HnPMT-catalyzed step might be a target for metabolic engineering of the TA production in H. niger .
研究领域:
植物学
·
代谢工程
·
分子生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD ENGINEERING
·
2022
·
IF 6.203
Yanqin Zhong,Mingliang Yang,Jingxin Chen,Hongbo Mi,Yonghong Ge,Jingyi Lv,Jianrong Li
The effect of putrescine as a pre-crosslinking agent on the performance of alginate film was investigated in the study. Compared to film pre-crosslinked with calcium ions (SA film), elongation at break (EB) of alginate film was effectively improved with putrescine pre-crosslinking. Specifically, the EB level of film pre-crosslinked with 0.05 mM putrescine increased by 11.9 times. Alginate films pre-crosslinked with putrescine had lower levels of swelling degree and water solubility than SA film. Additionally, the thermodynamic analysis revealed that alginate film pre-crosslinked with putrescine had higher thermal stability than SA film. These effects could be related to the formation of hydrogen and electrovalent bonds between putrescine and alginate molecules, as indicated by the Fourier transform infrared spectroscopy . Collectively, putrescine has potential as a pre-crosslinking agent to improve the physical properties of alginate film, and expand its application for food packaging.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL SCIENCES
·
2020
·Vol. 37
·期 2
·267-274
·
IF 2.049
Ghoto Saima Ameen,Khuhawar Muhammad Yar
A convenient and uncomplicated scheme has been projected for the quantitative determination of essential diamines putrescine (PUT) and cadaverine (CAD) via sodium dodecyl sulfate protected silver nanoparticles (SDS-AgNPs). This scheme is based on the chemical interaction of a SDS-AgNPs probe with PUT and CAD, leading to a color change from yellow to red or reddish brown. The interaction was investigated through different techniques such as using a UV-visible spectrophotometer, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), dynamic light scattering spectroscopy (DLS) and the zeta potential. Both amines possess a close resemblance in structure (except for the addition of one more methylene group in CAD), and no any distinguishable color change was noted. However, the maximum absorption band at 580 and 600 nm was demonstrated for PUT and CAD correspondingly. The methodical response was observed at absorption ratios of 580/410 and 600/410 nm, with the linear regression within 4–12 and 6–14 μg/mL for PUT and CAD. The detection limits calculated for both the diamines PUT and CAD were 0.333 and 1.638 μg/mL. The scheme was successfully applied for determinations in biological samples, including spiked blood plasma and urine. Putrescine exhibited % recovery within 95.717–105.200%, while cadaverine was within 95.940–105.109%, respectively. The scheme was reproducible and precise with inter-day RSD ( n = 5) within 1.126, 0.018% and the intraday RSD ( n = 5) was within 0.005, 0.002% for PUT and CAD, respectively.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Catalysts
·
2020
·Vol. 10
·期 9
·947
·
IF 3.52
Hongjie Hui,Yajun Bai,Tai-Ping Fan,Xiaohui Zheng,Yujie Cai
Putrescine, a biogenic amine, is a highly valued compound in medicine, industry, and agriculture. In this study, we report a whole-cell biocatalytic method inEscherichia colifor the production of putrescine, using L-arginine as the substrate. L-arginine decarboxylase and agmatine ureohydrolase were co-expressed to produce putrescine from L-arginine. Ten plasmids with different copy numbers and ordering of genes were constructed to balance the expression of the two enzymes, and the best strain was pACYCDuet-speB-speA. The optimal concentration of L-arginine was determined to be 20 mM for this strain. The optimum pH of the biotransformation was 9.5, and the optimum temperature was 45 °C; under these conditions, the yield of putrescine was 98%. This whole-cell biocatalytic method appeared to have great potential for the production of putrescine.Keywords:L-arginine;putrescine;Escherichia coli;whole-cell catalysis;co-expression
研究领域:
生物技术
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生物化学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Jing Wang,Min Du,Xin Wang,Junchen He,Alei Zhang,Kequan Chen
To achieve industrial-scale putrescine production, a high efficient bio-synthesis of putrescine involving arginase (ARG, EC 3.5.3.1) and L-ornithine decarboxylase was evaluated here. Among the four arginases tested, ARG BT from Bos Taurus showed the highest activity (1966 U/mg). Compared to the L-arginine decarboxylase (ADC) pathway, the strain expressing ARG BT and L-ornithine decarboxylase (SpeC) produced 28.7 g/L putrescine, a 38.6 % increase. Two pyridoxal phosphate (PLP) salvage pathways were evaluated, and the strain BL-P Tac -PdxK co-expressed pyridoxal kinase (PdxK) performed better. D-Glucose was used as the co-substrate to improve the putrescine titer further. Under optimal conditions, 43.6 g/L putrescine was produced from 87.1 g/L L-arginine, and 76 g/L putrescine was synthesized on a 0.5 L scale. Using L-arginine fermentation broth (60 g/L) as the substrate, a titer of 30 g/L putrescine was achieved. This efficient biotransformation process presented here enables feasible industrial-scale putrescine production.
研究领域:
生物工程
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 3.475
Guohui Li,Dixuan Huang,Li Wang,Yu Deng
Putrescine is an amine with high industrial value. At present, the chemical production method used in industry has shortcomings of using unsustainable petroleum raw material and expensive reaction catalyst, unsafe reaction process with inflammables and explosives. Therefore, researchers turn their attention to the biosynthesis of putrescine. Escherichia coli , with the natural pathway for the synthesis of putrescine, is a good choice for producing putrescine as the host strain. In this study, an effective putrescine bio-production platform was built via overexpressing two key pathway enzymes in engineered Escherichia coli PUT2, combining with a well-controlled fermentation strategy. Escherichia coli PUT2 converted arginine to putrescine within 48 h on a 3 L scale, reaching a titer of 26.21 g L −1 , and the specific production of putrescine was 1.99 g per unit biomass, with a productivity of 0.54 g L -1 h -1 , which obtained the high production efficiency of cell. Compared with the original strain Escherichia coli BL21(DE3), the putrescine titer increased to 79 times. This research offered a promising strategy for putrescine bio-synthesis, laying a foundation for large-scale putrescine bio-production.
研究领域:
生物技术
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微生物学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Food Measurement and Characterization
·
2021
·Vol. 15
·期 2
·1747-1753
·
IF 2.431
Li Yi-Fang,Lin Zheng-Zhong,Hong Cheng-Yi,Huang Zhi-Yong
A facile colorimetric method for rapid detection of putrescine (Put) and cadaverine (Cad) based on the peroxidase-like activity of (Fe,Co) codoped-CDs was developed. With the enzymatic hydrolysis by diamine oxidase (EC 1.4.3.6, DAO), Biogenic amines (BAs) were decomposed to produce H 2 O 2 which oxidizes 3,3',5,5'-tetramethyl-benzidine with the catalysis of (Fe,Co)-codoped CDs. By means of principal component analysis, Put and Cad were differentiated from BAs and their total amounts were detected by measuring the spectral absorption at 652 nm with a linear range from 0.25 to 10 mg kg −1 . The colorimetric method was applied to the detection of Put and Cad in various fish samples with a detection limit ((3σ/k, n = 9)) of 0.06 mg kg −1 . Besides verifying with HPLC, the recoveries of the colorimetric method were also tested by spiking standards at three levels with the range of 98.2%–115.7%, indicating that the established colorimetric method was accurate and sensitive.
研究领域:
食品科学
·
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2020
·
IF 4.613
Beitong Zhu,Lirong Jiang,Tianhong Chen,Guang-Ming Bao,Lintao Zeng,Xichao Hu,Hou-Qun Yuan
Biogenic primary diamines are a family of critical food spoilage biomarkers. To maintain food safety, it is necessary to implement highly efficient detection methods to monitor the levels of biogenic primary diamines in edible products. In this study, we report a 7-diethylaminocoumarin derivative, CMA-Cl, as a colorimetric and fluorescence lighting-up probe for detecting critical biogenic primary diamines including cadaverine and putrescine. The CMA-Cl probe underwent a nucleophilic substitution and condensation reaction with biogenic primary diamines, generating highly emissive compounds, which displayed strong emission at 475 nm with a fluorescence quantum yield of 36.4%. The CMA-Cl probe showed colorimetric and fluorescence dual-response towards biogenic primary amines with a significant linear relationship, good selectivity, and low limit of detection (209 nM). The probe demonstrated a good recovery (97–122%) for the determination of a typical biogenic primary diamine cadaverine during the spoilage of fish. Furthermore, CMA-Cl -loaded paper was fabricated for the real-time visual detection of a typical biogenic primary diamine cadaverine in fish samples based on distinct changes in color and fluorescence.
研究领域:
食品安全
·
分析化学
·
生物化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Xuan Qi,Wei-Feng Wang,Jun Wang,Jun-Li Yang,Yan-Ping Shi
A highly selective colorimetric detection method for putrescine on the basis of an optimized derivatization reaction was established. With the aids of o -phthalaldehyde (OPA) and thioglycolic acid (TGA), putrescine was derived to become red derivatives (PUT-RD), the form that can be detected qualitatively and quantitatively by visual inspection and UV-vis spectrophotometer at λ max of 490 nm, respectively. Key parameters affecting the experiment were investigated by one-factor-at-a-time and response surface analysis. At the optimal condition, this colorimetric method achieved good linearity at putrescine concentrations ranging from 0.8 to 200 μM with detection limit of 0.44 µM. The method also has good selectivity when common amino acids and inorganic ions, as well as ethylenediamine, 1,3-propanediamine, 1,5-pentanediamine, and 1,6-hexanediamine were used as interferences. The established colorimetric method was successfully employed for the detection of putrescine in 10 commercial fish products.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2025
·
IF 6.3
Huan Huang,Guocheng Zhou,Zhiyuan Meng,Ting Li,Linfeng Sun,Zhonglong Wang,Yiqin Yang
Biogenic amines (BAs), such as cadaverine (Cad), putrescine (Put), and spermine (Spe), serve as critical indicators of meat freshness. In this study, we developed a novel flavonol-derived fluorescent probe, 4-(3-((4-cyanobenzoyl)oxy)-4-oxo-2-(2,4,6-trimethoxyphenyl)-4H-chromen-7-yl)benzoic acid ( CTBA ). CTBA exhibited a sensitive and selective fluorescence response to BAs, with a pronounced emission peak at 510 nm that intensified proportionally with BA concentration. For Cad, the probe demonstrated a linear detection range of 0–45 μM, achieving a low limit of detection (LOD) of 0.36 μM. The interaction mechanism between CTBA and cadaverine was validated through 1 H NMR and high-resolution mass spectrometry (HRMS). Furthermore, a composite fluorescent film was fabricated by spin-coating CTBA with ethyl cellulose (EC), leveraging hydrogen-bonding interactions. This film was successfully applied for real-time monitoring of freshness in fish, and prawn. Our work introduces a pioneering bifunctional material capable of dual-purpose packaging and freshness detection, offering significant potential for enhancing food safety protocols.
研究领域:
食品安全
·
分析化学
·
传感器
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2019
·Vol. 136
·期 45
·48186
·
IF 2.188
Xiaoguang Ying,Anshun Kang,Xiaomei Zhu,Xiao Li
Electrospinning is an important tool for generating continuous fibers with large surface area and high pore ratio. The combination of nanofiber membrane and molecular imprinting can effectively measure specific adsorption of putrescine with coloring procedure. In this work, molecularly imprinted electrospun chromogenic membrane was synthesized with 1,4-butanediol (BDO) as imitate template. Poly(vinyl alcohol) (PVA) is used as spinning solution and ninhydrin as chromogenic agent for visualizing detection of target molecular putrescine. The adsorption concentration on molecularly imprinted polymer is illustrated by blue-purple colored stains and recorded by interactive three-dimensional surface plot of ImageJ. The results indicated that a uniform and bead-free PVA nanofiber was obtained using 10% (w/w) PVA solution, 15 kV voltage, 15 cm spinning distance, and 600 rpm roller speed. Also, the adsorption performance of molecular imprinting chromogenic membrane was investigated in detail, showing a higher adsorption capacity of 33.16 μg cm −2 and maximum imprinting efficiency of 2.30 under the best recognition conditions of 315 μL ninhydrin, 0.25% (w/w) BDO, and 0.4 cm sensing distance, as well as a short adsorption equilibrium time of 40 min. In addition, three amine competitors were discussed to reveal the better selectivity and higher specific recognition ability in the imprinted membrane. HIGHLIGHTS The molecular imprinting and coloring structure is constructed in nanofiber membrane with an enhanced specific surface area. The adsorption on imprinted membrane is visualized by chromogenic reaction between putrescine and ninhydrin to form a three-dimensional interactive surface proceeded using the ImageJ software. The imprinted nanofiber membrane with large area and high pore ratio is applied in detecting target in trace concentration within shortened time. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136 , 48186.
研究领域:
材料科学
·
分析化学
·
纳米技术
·
高分子科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Xiaohan Hua,Tingting Yan,Shuyi Liu,Lijun Yin,Xin Jia
The high protein content in sufu provides a substrate for microorganisms to produce biogenic amines (BAs), thereby posing a potential risk to food safety. The aim of this study was to identify the main BAs produced by different types of sufu (red, white and grey sufu) and investigate the relationship between bacterial diversity and BA production using high-throughput sequencing. The results revealed significant differences in the total BA content of the three types of sufu at the post-fermentation stage, with grey sufu exhibiting the highest total BA content (ranging from 6389.00 mg/kg to 7255.49 mg/kg). The main BAs found in all three types of sufu were tyramine, putrescine and cadaverine. Enterococcus genus produced high levels of tyramine and phenylethylamine in all three types of sufu. The predominant cadaverine-producing bacteria in red sufu was Enterobacter genus, whereas Klebsiella pneumoniae was the primary producer of cadaverine in white and grey sufu. Putrescine was primarily associated with the Bacillus and Enterobacter genera in red and grey sufu. Acinetobacter , Enterococcus and Enterobacter were the common dominant genera during the post-fermentation stage of the three kinds of sufu, which were positively correlated with the production of various BAs. These findings will help to understand the mechanism of BAs production by bacterial communities in different types of sufu to ensure safe production.
研究领域:
食品科学
·
微生物学
·
发酵技术
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）