FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Jingxin Ye,Weiqing Lan,Jing Xie
Exploring the roles of dominant bacteria to spoilage is important in inhibiting spoilage. This study investigated related quality, protein degradation, and muscle microstructure in refrigerated large yellow croaker inoculated with Shewanella putrefaciens (S) and Pseudomonas fluorescens (P) as mono- or co-culture (SP). P. fluorescens had the most robust spoilage ability. P. fluorescens and the co-culture degraded major myofibrillar proteins and the links to disrupt muscle fibers, induced significant free water loss in muscle and formed amounts of trichloroacetic acid (TCA)-soluble peptides and total volatile basic nitrogen (TVB-N). S. putrefaciens caused severe mutual separation in myofibers. The competitive interactions between the two spoilage bacteria inhibited spoilage in the co-culture, while the numbers of differentially abundant peptides in the co-culture were enriched. Therefore, this study revealed competitive interactions of the co-culture of S. putrefaciens and P. fluorescens , which offered a thorough understanding of bacterial spoilage behavior in large yellow croaker.
研究领域:
食品科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yujuan Qin,Pengcheng Huang,Fang-Ying Wu
With food safety problems abounding, it is essential to explore some efficient and effective methods for monitoring food quality. To this end, we have developed a histamine-responsive carbon dots (CDs)-based sensing system for visual monitoring of food spoilage. In this system, the aldehyde group on the surface of CDs serves as the recognition sites to combine with the amino group on histamine to form a Schiff base that effectively enhances the fluorescence. On the other hand, 1-aminoanthraquinone (ANQ) was introduced as a reference signal to endow the fluorescent probe with self-calibrating capacity. As the histamine concentration increases, linear ratiometric response was obtained accompanied with a distinguishable fluorescence color change from red to blue. Besides, test strips-based smartphone visual assays were successfully used to evaluate and monitor the histamine levels in fish samples. Our proposed sensing strategy has been demonstrated to be efficient and robust along with great potential for real-time visual monitoring of food spoilage.
研究领域:
食品安全
·
生物传感
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Sensors & Diagnostics
·
2025
·
IF 4.1
Teody Gumabat,Jeanne Phyre Lagare Oracion,Jolina Fedelis,Ethel Keleste,Rey Capangpangan,Noel Lito Sayson,Gerard Dumancas,Arnold Alguno,Felmer Latayada
Spermine, a key biogenic amine indicating food freshness, is typically detected using costly and time-consuming chromatographic methods. This study introduces a more efficient, eco-friendly alternative—a label-free colorimetric sensing platform using gold nanoparticles (AuNPs) functionalized with 11-mercaptoundecanoic acid and capped with an N-hydroxysuccinimide (NHS)-ester. Transmission electron microscopy revealed monodisperse, spherical AuNPs (13 nm), with an increase in size upon functionalization. Fourier transform infrared spectra confirmed successful functionalization. The hydrodynamic size of the AuNPs increased from 17.26 nm to 2167 nm, and the zeta potential shifted from −48.86 mV to −35.59 mV. The platform takes advantage of the selective interaction between spermine and NHS-ester-functionalized AuNPs, inducing nanoparticle aggregation, as shown by a red shift in surface plasmon resonance (SPR). UV-vis spectroscopy demonstrated a robust linear correlation (R2 = 0.958) between spermine concentration (1.0–4.0 μM) and nanoparticle aggregation index, with a limit of detection (LOD) of 0.77 μM. The sensor also exhibited high reproducibility in pork extract matrices (coefficient of variation <5%) and selectivity for spermine amid various interfering analytes. Its eco-friendly design and rapid response time position it as a viable tool for real-time spermine monitoring in food spoilage, offering comparable performance metrics to traditional chromatographic techniques while addressing sustainability concerns.
研究领域:
生物化学
·
食品安全
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
CyTA-Journal of Food
·
2021
·
IF 2.255
Feng Lv,Chenhui Zhong,Shuiqing Wu,Linghua Wang,Haiyan Wu,Jiang Sun
We assessed the changes in nutritional and putrescent components during enzymolysis of Acetes chinensis. Oligopeptide and astaxanthin both reached a maximum of 46.78 ± 1.03% and 14.71 ± 0.32 mg/kg at 6 h, respectively. The total free amino acid changed from 6.37 ± 0.08 g/100 g to 14.21 ± 0.17 g/100 g at 8 h. However, the volatile base nitrogen reached 143.50 ± 6.21 mg/100 g at 10 h. The total biogenic amine was first decreased and then increased to 6,324.00 ± 10.56 mg/kg. Moreover, the acid and peroxide values reached 4.93 ± 0.17 mg/g and 5.56 ± 0.23 mg/kg at 10 h, respectively. Therefore, although Acetes chinensis hydrolysate was rich in nutrients, the putrescent components, including volatile base nitrogen, biogenic amine, acid and peroxide values, were increased during enzymolysis. Collectively, the enzymatic hydrolysis process of Acetes chinensis should be appropriately regulated to reduce the contents of putrescent components.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
FOOD MICROBIOLOGY
·
2024
·
IF 4.5
Mengyuan Qin,Shuo Han,Miaomiao Chen,Pengju Li,Yuqi Wang,Wenfang Niu,Chao Gao,Hailei Wang,Yi Li
Hafnia paralvei , a Gram-negative foodborne pathogen, is found ubiquitously in various aquatic animals and seafoods, which can form biofilm as a dominant virulence factor that contributes to its pathogenesis. However, the biofilm formation mechanism of H. paralvei and its effect on food spoilage has not been fully characterized. Here we show that biofilm formation, is regulated by c-di-GMP which mediated by bcsB , can increase the spoilage ability of H. paralvei . We found that GTP was added exogenously to enhance the synthesis of c-di-GMP, which further promoted biofilm formation. The gene dgcC , one of 11 genes encoding GGDEF domain-containing proteins in H. paralvei , was significantly upregulated with GTP as substrate. The upregulation of dgcC contributes to a significant increase of c-di-GMP and the formation of biofilm. In addition, the overexpression of dgcC induced upregulation of bcsB , a reported effector protein encoding gene, which was further demonstrated that overexpression of bcsB can encourage the synthesis of bacterial cellulose and biofilm formation. The effect of biofilm formation induced by c-di-GMP on spoilage of Yellow River carp ( Cyprinus carpio ) was evaluated by sensory evaluation , the total viable count , and the total volatile basic nitrogen, which showed that biofilm formation can significantly increase the spoilage ability of H. paralvei on C. carpio . Our findings provide the regulation of c-di-GMP on expression of bcsB , that can contribute to biofilm formation and spoilage ability of H. paralvei , which is favor to understanding the pathogenesis of Hafnia paralvei and its role in food spoilage.
研究领域:
微生物学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2020
·
IF 4.236
Wang Xin-Yun,Xie Jing
Food spoilage by certain species of bacteria is reported to be regulated by quorum sensing (QS). Acinetobacter johnsonii and Pseudomonas fluorescens, the major specific spoilage organisms, are found to be limited in their QS and co-culture interactions. The aim of this study was to determine how QS-regulated proteins affect the spoilage potential of co-cultured A. johnsonii and P. fluorescens obtained from spoiled bigeye tuna (Thunnus obesus) using a proteomics approach. The A. johnsonii, P. fluorescens, and their co-culture tested the N-acyl-homoserine lactone (AHL) activities using reporter Chromobacterium violaceum CV026 and LC-MS/MS in qualitative and quantitative approaches, respectively. These latter showed that, of the 470 proteins and 444 proteins in A. johnsonii (A) and P. fluorescens (P), respectively, 80 were significantly up-regulated and 97 were significantly down-regulated in A vs. AP, whereas 90 were up-regulated and 65 were down-regulated in P vs. AP. The differentially expressed proteins included the AI-2E family transporter OS, 50S ribosomal protein L3, thioredoxin reductase OS, cysteine synthase CysM OS, DNA-binding response regulator, and amino acid ABC transporter ATPase OS. The cellular process (GO:0009987), metabolic process (GO:0008152), and single-organism process (GO:0044699) were classified into the gene ontology (GO) term. In addition, energy production and conversion, amino acid transport and metabolism, translation, ribosomal structure and biogenesis, post-translational modification, protein turnover, and chaperones were distributed into the clusters of orthologous groups of proteins (COG) terms. The KEGG pathways revealed that 84 and 77 differentially expressed proteins were divided into 20 KEGG pathways in A vs. AP and P vs. AP, respectively, and amino acid metabolism, carbohydrate metabolism, energy metabolism, and translation were significantly enriched. Proteins that correlated with the spoilage-related metabolic pathways, including thioredoxin reductase OS, cysteine synthase OS, and pyridoxal phosphate-dependent enzyme family protein OS, were identified. AI-2E family transporter OS and LuxR family transcriptional regulator OS were identified that related to the QS system. These findings provide a differential proteomic profile of co-culture in A. johnsonii and P. fluorescens, and have potential applications in QS and the regulation of spoilage potential.
研究领域:
食品科学
·
微生物学
·
蛋白质组学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Liangting Shao,Yang Dong,Shanshan Chen,Junsheng Sheng,Linlin Cai,Xinglian Xu,Huhu Wang
Aeromonas is a ubiquitous aquatic bacteria, and it is a significant factor contributing to meat spoilage during processing and consumption. The abilities of Aeromonas salmonicida 29 and 57, which exhibit spoilage heterogeneity, to secrete protease, lipase, hemolysin, gelatinase, amylase, and lecithinase were confirmed by plate method. A total of 3948 proteins were identified by ITRAQ in extracellular secretions of A. salmonicida , and 16 proteases were found to be potentially related to spoilage ability. The complete genome sequence of A. salmonicida 57 consists of one circular chromosome and three plasmids, while A. salmonicida 29 consists of one circular chromosome, without a plasmid. Transcriptomic analysis revealed a significant number of DEGs were up-regulated in A. salmonicida 29, which were mainly enriched in metabolic pathways (e.g., amino acid metabolism, carbohydrate metabolism), indicating that A. salmonicida 29 had better potential to decompose and utilize nutrients in meat. Six protease genes (2 pepB , hap , pepA , ftsI , and pepD ) were excavated by combined ITRAQ with transcriptome analysis, which potentially contribute to bacterial spoilage ability and exhibit universality among other dominant spoilage bacteria. This investigation provides new insights and evidence for elucidating metabolic and spoilage phenotypic differences and provides candidate genes and strategies for future prevention and control technology development.
研究领域:
微生物学
·
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Kai Xu,Lewei Gao,Yueyin Liang,Zhiyuan Meng,Shuai Gong,Zhonglong Wang,Shifa Wang
Hydrogen sulfide (H 2 S) has multiple contributions to living organisms and is also linked to the freshness of most high-protein foodstuffs. Therefore, developing a highly sensitive technology for on-site detection of H 2 S is promising and crucial. In this paper, a novel ratiometric near-infrared fluorescent probe 1,1,3-trimethyl-2-(4-(5,5,7-trimethyl-4,5,6,7-tetrahydro-3 H -4,6-methanobenzo[ d ]imidazol-2-yl)styryl)− 1 H -benzo[ e ]indol-3-ium iodide ( TIBI ) was designed and synthesized from α -pinene derivative 4,6,6-trimethylbicyclo[3.1.1]heptane-2,3-dione for the specific detection of H 2 S. TIBI displayed several excellent merits during the detection of H 2 S including low detection limit (19.43 nM), short response time (50 s), and wide pH detection range (5 9). The emission wavelength of TIBI blue-shifted from 690 nm to 460 nm accompanying with the fluorescence color changed from red to blue at the presence of H 2 S, and its fluorescence intensity ratio (I 460 nm /I 690 nm ) enhanced up by 540 folds. The TIBI -loaded test strips could successfully achieved the real-time and convenient monitoring of H 2 S produced from food spoilage . Besides, probe TIBI could be also applied for imaging H 2 S level in living cells and zebrafish.
研究领域:
生物化学
·
分析化学
·
食品安全
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2025
·
IF 3.3
Xu Zhenbo,Chen Rong,Wu Jie,Zhong Feifeng,Xue Haoyue,Xiang Zhufang,Yang He,Yuan Lei,Liu Junyan,Yu Junhong
Lacticaseibacillus paracasei is a typical food spoilage bacterium especially in beer. Due to the negative impact of L. paracasei on food preservation, a rapid detection method for L. paracasei based on polymerase spiral reaction (PSR) was established and its application was verified using different types of food samples in this study. Firstly, target digging was performed using bioinformatics techniques. A target gene pheS was selected and species-specific primers were designed. Secondly, the PSR assay was established with chromogenic reaction, which avoid false positive result due to open lid. The specificity of target and primers was tested, and the limit of detection (Kolodkin-Gal in Science 328:627–629, 2010) (5.2 × 10 4 fg/μL) was examined. Thirdly, the application of PSR assay in solid and liquid food samples were verified by artificial contamination model. The PSR assay was more efficiency in liquid food (LOD: 10 3 CFU/mL) than in solid food (LOD: 10 4 CFU/mL). Thus, L. paracasei screening in 3 types of beer (LOD: 10 3 CFU/mL) was further performed to verify to efficiency of PSR assay in screening of beer spoilage bacteria. In conclusion, pheS targeting PSR assay is useful as a rapid detection method for L. paracasei in solid and liquid food samples especially in beer.
研究领域:
微生物学
·
食品安全
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
TheScientificWorldJOURNAL
·
2013
·
IF 1.73
Jun Tian,Xiaobin Zeng,Hong Zeng,Zhaozhong Feng,Xiangmin Miao,Xue Peng
The antifungal efficacy of nerol (NEL) has been proved against Aspergillus flavus by using in vitro and in vivo tests. The mycelial growth of A. flavus was completely inhibited at concentrations of 0.8 μL/mL and 0.1 μL/mL NEL in the air at contact and vapor conditions, respectively. The NEL also had an evident inhibitory effect on spore germination in A. flavus along with NEL concentration as well as time-dependent kinetic inhibition. The NEL presented noticeable inhibition on dry mycelium weight and synthesis of aflatoxin B1 (AFB1) by A. flavus, totally restraining AFB1 production at 0.6 μL/mL. In real food system, the efficacy of the NEL on resistance to decay development in cherry tomatoes was investigated in vivo by exposing inoculated and control fruit groups to NEL vapor at different concentration. NEL vapors at 0.1 μL/mL air concentration significantly reduced artificially contaminated A. flavus and a broad spectrum of fungal microbiota. Results obtained from presented study showed that the NEL had a great antifungal activity and could be considered as a benefit and safe tool to control food spoilage.
研究领域:
微生物学
·
食品安全
·
植物化学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2016
·
IF 3.445
Faizan A. Sadiq,Yun Li,TongJie Liu,Steve Flint,Guohua Zhang,Lei Yuan,Zhipeng Pei,GuoQing He
The propensity for aerobic bacilli and allied genera to produce highly heat-resistant spores and thermally stable spoilage enzymes are major bacteriological issues faced by the dairy industry. Most of the enzymes are able to survive any heat treatment applied during the manufacture of milk powders and have the potential to remain active in milk powders and other dairy products during storage, and may explain some of the sensory and functionality defects reported in dairy products . Despite many reports on the occurrence of spore-forming bacteria in dairy products, knowledge about food quality related properties of many aerobic sporeformers is still scarce. Therefore, the aim of this study was to determine thermal resistance and spoilage potential of a large pool of mesophilic and thermophilic sporeformers, representing 738 isolates and 31 different RAPD groups, recently isolated from Chinese milk powders. Spore formers producing highly heat resistant spores (surviving 125 °C for 30 min) included 2 thermophiles ( Geobacillus thermoleovorans group and Geobacillus stearothermophilus ) and one mesophilic species ( Brevibacillus brevis ). Paenibacillus macerans showed the highest proteolytic activity followed by members of the Bacillus cereus group, Br. brevis , Bacillus subtilis , G. thermoleovorans group and Virgibacillus proomii . The highest lipase producing strains belonged to Bacillus licheniformis . Phospholipase activity was only shown by members of the B. cereus group and Brevibacillus parabrevis . Ten strains showed positive β -galactosidase activity, while, 4 strains showed positive haemolytic activity. B. licheniformis strains, despite belonging to one RAPD group or sub-group showed markedly different phenotypic characters which support the previous findings of heterogeneity in RAPD-based B. licheniformis groups. The results of this study will broaden the knowledge about the spoilage potential and thermal resistance of many strains of dairy origin.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Jiaying Geng,Zhongxi Huang,Qian Shen,Chenfeng Xu,Long Zhang,Cuilan Wei,Chan Fu,Weizhen An,Changmin Yu
With the emergence of numerous food safety problems, rapid and accurate detection of histamine in food spoilage remains a challenge. To this end, we developed a simple design and easy synthesis of fluorescein-based probe FCHO to achieve specific and rapid (<1 s) quantitative detection of histamine through “imine formation” reaction. Significant enhanced fluorescence signal in response to histamine enabled our probe with high sensitivity as low as 51 nM. Utilizing the visualized fluorescence color changes of the probe as histamine increasing, we combined it with paper-based test chip to construct a color-resolved and highly selective recognition system. In addition, our proposed probe has been successfully used to visually imaging histamine changes in fish samples. Finally, for the first time, we have proved it possesses reliable ability to directly in situ imaging the distribution of histamine in whole spoiled fish. Thus, our strategy will provide great potential for monitoring food spoilage.
研究领域:
食品安全
·
分析化学
·
生物传感
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）