ANALYTICAL CHEMISTRY
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2017
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IF 6.32
Juan Zhang,Xiaonan Wang,Tingjun Chen,Chang Feng,Genxi Li
In this work, a new strategy for electrochemical analysis of enzyme has been proposed based on a self-assembled lipid bilayer on an electrode surface mediated by hydrazone chemistry. Taking aldolase as an example, the enzyme can catalyze the formation of products containing carbonyl groups. These groups can react with hydrazine groups of the functional lipid derivative, resulting in the self-assembly of a lipid bilayer on a guanidinium modified electrode surface. The lipid bilayer will then prevent the movement of hydrophilic electrochemical probes. Consequently, the catalytic reaction of the enzyme may result in the change of the obtained electrochemical peak current. Experimental results reveal that aldolase activity can be analyzed over a widely linear detection range from 5 mU/L to 100 U/L with a low detection limit of 1 mU/L. Meanwhile, the method can exhibit good precision and reproducibility and it can be applied for real sample analysis. What is more, because the lipid bilayer is the universal basis for cell-membrane structure, while hydrazone chemistry is popular in nature, this work may also provide a new insight for the development of electrochemical analysis and electrochemical biosensors.
研究领域:
生物化学
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2020
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IF 5.977
Zhouxian Li,Qi Wang,Jiawei Mao,Lingyi Zhang,Weibing Zhang,Mingliang Ye
A challenge for shotgun proteomics is the identification of low abundance proteins, which is always hampered owing to the extreme complexity of protein digests and highly dynamic concentration range of proteins. To reduce the complexity of the peptide mixture, we developed a novel method to selectively enrich N-terminal proline peptides via hydrazide chemistry . This method consisted of ortho -phthalaldehyde (OPA) blocking of primary amines in peptides, reductive glutaraldehydation of N-terminal proline and solid phase hydrazide chemistry enrichment of aldehyde-modified N-terminal proline peptide. After enrichment, the number of detected peptides containing N-terminal proline increased from 1304 to 4039 and the ratio of N-terminal proline peptides jumped from 4.4% to 93.7%, showing good enrichment specificity towards N-terminal proline peptides. Besides, the ratio of identified peptides to proteins was decreased from 7.8 (29751/3811) to 1.5 (4347/2821), indicating that sample complexity was drastically reduced through this method. As a result, this novel approach for enriching N-terminal proline peptides is effective in identification of low abundance protein owing to the reduction of sample complexity.
研究领域:
生物化学
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蛋白质组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2024
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IF 12.2
Jingyi Yang,Yining Zhao,Lelin Qian,Ying Yu,Yuan Zhang,Juan Zhang
In this work, a dual recognized CRISPR/Cas12a system has been proposed, in which the activation chain is cleverly divided into two parts that can serve for precise dual target recognition, and hydrazone chemistry is introduced for the formation of a whole activation chain. It has been further explored to construct a new method for the specific and sensitive detection of Staphylococcus aureus (SA) as one of the most common pathogens in infectious diseases. In virtue of proximity effect contributed by complementary base pairing, hydrazone chemistry accelerates the formation of the whole activation strand and improves the specificity of the CRISPR/Cas12a system, serving for the accurate analysis of SA. Moreover, the temporary aggregation of CRISPR/Cas12a around SA enhances its catalytical efficiency so as to further amplify signal. With high sensitivity, stability, reproducibility and specificity, the established method has been successfully applied to detect SA in complex substrates. Meanwhile, our established method can well evaluate the inhibition effect of chlorogenic acid and congo red in comparison with flow cytometry. Environmental implication Bacterial pathogens exist widely in the environment and seriously threaten the safety of human health. Staphylococcus aureus (SA) is the most common pathogen of human suppurative infection, which can cause local suppurative infection, pneumonia, and even systemic infections such as sepsis. In this work, a dual recognized CRISPR/Cas12a system mediated by hydrazone chemistry has been proposed. With high sensitivity and low detection limit, the established method can specifically detect SA and effectively evaluate the antibacterial effect of inhibitors. This method is expected to be further developed into a detection method in different scenarios such as environmental monitoring and clinical diagnosis.
研究领域:
分子生物学
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病原微生物学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
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2023
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IF 5
Guangxuan Yang,Jian Liu,Yi Zhang,Jinfeng Yuan,Mingwang Pan,Zhicheng Pan
Zwitterionic hydrogels have shown promise as effective antifouling materials in wound dressing applications owing to their robust hydrate layer that offers protection against microbial infections. However, the traditional covalently crosslinked zwitterionic hydrogels may not be sufficient to adapt to irregular injury sites and fully address microbial infections within the wound. Herein, we propose an injectable zwitterionic hydrogel that utilizes dynamic hydrazone chemistry via aldehyde and hydrazide functional groups and contains N-halamine antibacterial agents. The dual dynamic exchangeable covalently and physically crosslinked structure resulting from the combination of hydrazone bonds and zwitterion interactions facilitates instantaneous gelation (within a few seconds) and extreme stability without any degradation (for over 2 months). The hydrogels exhibit remarkable fouling resistance against proteins and bacteria while demonstrating excellent antibacterial performance in vitro without compromising cytocompatibility. More importantly, we demonstrate their inherent conductivity properties and sensitivity to external pressure. The combination of the injectability, antifouling, antibacterial, cytocompatibility, and electrical conductivity properties achieved offers the potential to expand the utility of zwitterionic hydrogels in biological applications.
研究领域:
材料科学
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生物材料
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生物医学工程
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化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）