Stem Cell Research & Therapy
·
2020
·Vol. 11
·期 1
·1-11
·
IF 5.116
Yang Zhi,Song Zhicheng,Nie Xin,Guo Kaijin,Gu Yan
Background Biological composite scaffolds are increasingly being used in abdominal wall reconstruction but still have certain shortcomings. The present study describes here a novel three-dimensional (3D) scaffold fabricated by combining 3D printing (3DP) and electrospinning (ESP). Methods Biological composite scaffolds are composed of integrated 3DP interconnected macrofiber and random ESP microfiber networks. The 3DP scaffold retains intact 3D architecture and mechanical properties, while the ESP network serves as a cell entrapment system at the extracellular matrix (ECM) scale. Biological composite scaffolds are implanted in a defective rat abdominal wall to detect if it could induce early vascularization and reconstruction of the tissue defect. Results SEM analysis reveals a pore diameter of 424.47 ± 58.49 μm and a porosity of 70.46 ± 2.48% for biological composite scaffolds. In the in vitro test of cell proliferation, biological composite scaffolds do not affect rat dermal fibroblast proliferation in a time- and dose-dependent manner. The animal experiments show tissue remodeling and early angiogenesis as compared to 3DP scaffolds. Conclusions Our experiment prepares a biological scaffold with both a macro- and microscale structure by ESP and 3DP technology. Thus, the integration of 3DP and ESP techniques provides a new set of smart scaffolds for abdominal wall defect and hernia repair.
研究领域:
生物材料学
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组织工程学
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2025
·
IF 5.4
Xinghan Wu,Wenxin Xu,Xuantao Huang,Nianhua Dan,Yining Chen,Zhengjun Li,Yunbing Wang
Emergency repair of complicated full-thickness abdominal wall defects remains one of the most common and challenging surgical emergencies globally. Here, an integrated polydopamine permeating-cross-linking strategy was innovatively proposed to convert porcine acellular dermal matrix (pADM) into versatile, degradation-resistant biopatches (PDA-pADM) for efficiently repairing full-thickness abdominal wall defects. The strategy significantly addresses the challenge that natural-property improvement and biocompatibility of biomaterials are difficult to balance. Molecularly, dopamine (DA) molecules could fully permeate into the collagen fibers of the acellular dermal matrix and then automatically trigger the interfacial in situ polymerization of dopamine monomers among collagen fibers to achieve the efficient cross-linking of pADM. Surprisingly, the enzymatic durability of the biopatch shows significant improvements, extending the original duration from 3 to 20 d. Comprehensive in vivo experiments have shown that PDA-pADM can effectively promote angiogenesis and inhibit inflammatory response, so as to achieve regeneration and repair of abdominal wall damage.
研究领域:
生物医学工程
·
组织工程
·
外科手术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2024
·
IF 5.8
Renquan Xing,Rui Gao,Yini Huangfu,Yufeng Zhang,Shuangyang Li,Chuangnian Zhang,Pingsheng Huang,Weiwei Wang,Anjie Dong,Zujian Feng
Current mesh materials used for the clinical treatment of abdominal defects struggle to balance mechanical properties and bioactivity to support tissue remodeling. Therefore, a bioactive microgel coated electrospinning membrane was designed with the superiority of cell-instructive and topology in guiding cell behavior and function for abdominal wall defect reconstruction. The electrostatic spinning technique was employed to prepare a bioabsorbable PLCL fiber membrane with effective mechanical support. And decellularized matrix (dECM) derived bioactive microgels were further coated on the fiber membrane through co-precipitation with dopamine, which was expected to endow with cell-instructive hydrophilic interfaces, and also topological morphologies for cell adhesion. Moreover, the introduction of dECM into the microgel was able to promote myogenic proliferation and differentiation of C2C12 cells. Subsequently, in vivo experiments using a rat abdominal wall defect model demonstrated the bioactive microgel coating significantly contributed to the reconstruction of intact abdominal wall structures, highlighting its potential for clinical application in promoting the repair of soft tissue defects associated with abdominal wall damage. This study presented an effective mesh material for facilitating the reconstruction of abdominal wall defects, and also contributed novel design concepts for surface modification of scaffolds with cell-instructive interfaces and topology.
研究领域:
生物医学工程
·
组织工程
·
材料科学
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Xiang Zhang,Xiaoning Liu,Mohan Wang,Jingjing Zhang,Ke Liu,Ziming Xu,Wanfeng Chen,Jun Hu,Pan Zhang,Yinshun Zhang,Liang Dong,Weiping Xu,Zhao Pan
Point-wise suturing is the standard method for ensuring that patches effectively perform mechanically supportive functions in tissue repair. However, stress concentrations around suture holes can compromise the mechanical stability of patches. In this study, we develop a suturable hydrogel patch with flaw-tolerance capabilities by leveraging multiscale stress deconcentration, inspired by natural silk. This design mitigates stress concentration across two scales through the synergistic integration of nanoscale high-energy crystalline domains and intermolecular interactions. The resulting integral hydrogel patch exhibits superior flaw resistance compared to conventional patches and effectively addresses tissue adhesion issues. To validate the efficacy of the patch, we demonstrate successful in vivo repair of abdominal wall defects in rats, comparing the performance of the proposed patch to commercial mesh patches (Prolene). The integral patch design strategy present here offers a valuable approach for developing patches that can be tailored to meet the mechanical support needs of various tissue repair applications.
研究领域:
生物材料学
·
组织工程
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Fiber Materials
·
2025
·
IF 21.3
Tao Yinghua,Luo Peiyu,Jing Fengya,Liu Tao,Tan Xin,Lyu Zhiyang,VeerleBernaerts Katrien,Zhang Tianzhu,Jia Ruipeng
Repairing abdominal wall defects presents significant challenges, due to the high infection risk, poor biocompatibility, and insufficient mechanical strength associated with synthetic materials. To overcome these limitations, we developed a bioinspired multifunctional 3DPF patch by integrating 3D printing and electrospinning technologies. The core material of the patch is 4arm-PLGA-GPO (4A-GPO), synthesized by conjugating the Gly-Pro-Hyp (GPO) peptide sequence with 4arm-PLGA(4A), which significantly enhances bioactivity and mechanical properties. Additionally, the patch encapsulates basic fibroblast growth factor (bFGF) to stimulate cell proliferation and migration, while an antibacterial layer composes of emodin (EMO) and tobramycin to prevent infection. In vivo studies demonstrate the 3DPF patch effectively accelerates tissue repair by reducing fibrosis and adhesions, promoting angiogenesis and collagen deposition, and modulating the immune response. Transcriptomic analysis reveals that the patch downregulates IL-17 mediated inflammatory pathways while upregulating cell adhesion molecule-related pathways, synergistically facilitating microenvironment reconstruction. Furthermore, molecular docking studies suggest the patch interacts with key molecules such as VEGF and COL3, enhancing angiogenesis and matrix remodeling. In summary, this biomimetic patch, composed of bioactive materials with well-defined chemical compositions, integrates mechanical support, immune modulation, and antibacterial protection. by offering a comprehensive solution for abdominal wall repair, it holds significant potential for clinical translation in complex tissue engineering applications.
研究领域:
生物材料学
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组织工程学
·
再生医学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
Dong Wenpei,Song Zhicheng,Liu Suihong,Yu Ping,Shen Zhipeng,Yang Jianjun,Yang Dongchao,Hu Qinxi,Zhang Haiguang,Gu Yan
Application of synthetic or biological meshes is the main therapy for the repair and reconstruction of abdominal wall defects, a common disease in surgery. Currently, no ideal materials are available, and there is an urgent need to find appropriate ones to satisfy clinical needs. Electrospun scaffolds have drawn attention in soft tissue reconstruction. In this study, we developed a novel method to fabricate a composite electrospun scaffold using a thermoresponsive hydrogel, poly (N-isopropylacrylamide)-block-poly (ethylene glycol), and a biodegradable polymer, polylactic acid (PLA). This scaffold provided not only a high surface area/volume ratio and a three-dimensional fibrous matrix but also high biocompatibility and sufficient mechanical strength, and could simulate the native extracellular matrix and accelerate cell adhesion and proliferation. Furthermore, rat adipose-derived stem cells (ADSCs) were seeded in the composite electrospun scaffold to enhance the defect repair and regeneration by directionally inducing ADSCs into endothelial cells. In addition, we found early vascularization in the process was regulated by the hypoxia inducible factor-1α (HIF-1α)/vascular endothelial growth factor (VEGF) pathway. In our study, overexpression of HIF-1α/VEGF in ADSCs using a lentivirus system promoted early vascularization in the electrospun scaffolds. Overall, we expect our composite biomimetic scaffold method will be applicable and useful in abdominal wall defect regeneration and repair in the future.
研究领域:
组织工程
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生物材料
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再生医学
·
外科研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2023
·
IF 4.6
Xiuxia Sun,Qi Chen,Angelique A. Guan,Shuaishuai Yuan,Zhibo Li
Abdominal wall defect caused by surgical trauma, congenital rupture, or tumor resection may result in hernia formation or even death. Tension-free abdominal wall defect repair by using patches is the gold standard to solve such problems. However, adhesions following patch implantation remain one of the most challenging issues in surgical practice. The development of new kinds of barriers is key to addressing peritoneal adhesions and repairing abdominal wall defects. It is already well recognized that ideal barrier materials need to have good resistance to nonspecific protein adsorption, cell adhesion, and bacterial colonization for preventing the initial development of adhesion. Herein, electrospun poly(4-hydroxybutyrate) (P4HB) membranes infused with perfluorocarbon oil are used as physical barriers. The oil-infused P4HB membranes can greatly prevent protein attachment and reduce blood cell adhesion in vitro. It is further shown that the perfluorocarbon oil-infused P4HB membranes can reduce bacterial colonization. The in vivo study reveals that perfluoro(decahydronaphthalene)-infused P4HB membranes can significantly prevent peritoneal adhesions in the classic abdominal wall defects’ model and accelerate defect repair, as evidenced by gross examination and histological evaluation. This work provides a safe fluorinated lubricant-impregnated P4HB physical barrier to inhibit the formation of postoperative peritoneal adhesions and efficiently repair soft-tissue defects.
研究领域:
生物医学工程
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材料科学
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外科实践
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组织工程
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生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
He Gan,Lin Wang,Ran Sun,Junchen Li,Zhirong Zhao,Yuan Chen,Weiliang Tian,Fan Yang,Qian Huang
Polyurethane has been widely utilized in medical devices, owing to its excellent biocompatibility, mechanical properties, and processability. This study presents a thermoresponsive self-healing waterborne polyurethane elastomer (WPU) incorporating graphene oxide (GO), designed to enhance the repair of abdominal wall defects. This material integrates multiple functionalities including antimicrobial activity, tissue regeneration, and self-healing capabilities, offering a safer and more effective solution. The WPU matrix provides excellent biocompatibility and controllable degradability, contributing to reduced tissue adhesion and promoted abdominal wall regeneration. The incorporation of GO endows the material with photothermal properties, enabling localized thermal effects under near-infrared irradiation to achieve synergistic antimicrobial effects. Furthermore, the dynamic chemical bonds in the cross-linked network confer thermoresponsive self-healing ability. In infected skin and abdominal wall defect rat models, the WPU-GO elastomer exhibited potent antimicrobial activity, promoted wound closure, reduced inflammation, and demonstrated excellent antiadhesion and tissue regeneration capabilities. This multifunctional material shows remarkable potential in abdominal wall defect treatment.
研究领域:
生物材料
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高分子科学
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生物医学工程
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纳米技术
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再生医学
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伤口愈合
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Ran Sun,Lei Lei,Jiamin Ji,Yuan Chen,Weiliang Tian,Fan Yang,Qian Huang
In abdominal wall defect repair, surgical site infection (SSI) remains the primary cause of failure, while complications like visceral adhesions present significant challenges following patch implantation. We designed a Janus multifunctional hydrogel patch (JMP) with antibacterial, anti-inflammatory, and anti-adhesive properties. The patch comprises two distinct layers: a pro-healing layer and an anti-adhesion layer. The pro-healing layer was created by a simple mixture of polyvinyl alcohol (PVA), quaternized chitosan (QCS), and gallic acid (GA), crosslinked to form PVA/QCS/GA (PQG) hydrogels through GA's self-assembly effect and hydrogen bonding. Additionally, the PVA anti-adhesive layer was constructed using a drying-assisted salting method, providing a smooth and dense physical barrier to prevent visceral adhesion while offering essential mechanical support to the abdominal wall. The hydrogel patch demonstrates widely adjustable mechanical properties, exceptional biocompatibility, and potent antimicrobial properties, along with a sustained and stable release of antioxidants. In rat models of skin and abdominal wall defects, the JMP effectively promoted tissue healing by controlling infection, inhibiting inflammation, stimulating neovascularization, and successfully preventing the formation of visceral adhesions. These compelling results highlight the JMP's potential to improve the success rate of abdominal wall defect repair and reduce surgical complications.
研究领域:
生物医学工程
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材料科学
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组织工程
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外科研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Qingxi Hu,Yu Zhang,Yongteng Song,Hekai Shi,Dongchao Yang,Haiguang Zhang,Yan Gu
The application of patch methods for repairing abdominal wall wounds presents a variety of challenges, such as adhesion and limited mobility due to inadequate mechanical strength and nonabsorbable materials. Among these complications, postoperative visceral adhesion and wound infection are particularly serious. In this study, a bilayered composite patch with a gelatin methacryloyl (GelMA)/sodium alginate (SA)-vancomycin (Van)@polycaprolactone (PCL) (GelMA/SA-Van@PCL) antibacterial layer was prepared via coaxial 3D printing and a polycaprolactone (PCL)-silicon dioxide (SiO2) antiadhesive layer (PCL-SiO2) was prepared via electrospinning and electrostatic spray for hernia repair. The evaluation of the physicochemical properties revealed that the composite patch had outstanding tensile properties (16 N cm−1), excellent swelling (swelling rate of 243.81 ± 12.52%) and degradation (degradation rate of 53.14 ± 3.02%) properties. Furthermore, the composite patch containing the antibiotic Van exhibited good antibacterial and long-term drug release properties. Both in vivo and in vitro experiments indicated that the composite patch displayed outstanding biocompatibility and antiadhesive properties and could prevent postoperative infections. In summary, the bilayered composite patch can effectively prevent postoperative complications while promoting tissue growth and repair and holds significant application potential in hernia repair.
研究领域:
生物医学工程
·
材料科学
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组织工程
·
外科研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2021
·Vol. 9
·期 23
·7895-7910
·
IF 6.843
Guangxiu Cao,Wei He,Yubo Fan,Xiaoming Li
The repair of abdominal wall defects is currently a clinical challenge. A naturally derived extracellular matrix (ECM) such as small intestine submucosa (SIS) has received great attention in abdominal wall defect repair because of its remarkable bioactivity, biodegradability and tissue regeneration. The match between material degradation and tissue remodeling is very important for the realization of ideal repair effectiveness. In this study, a near-infrared (NIR) fluorescent dye Cy5.5 NHS ester was used to label ECM-based (ECMB) composites consisting of SIS and chitosan/elastin electrospun nanofibers for monitoring material degradation. The tissue remodeling in the ECMB composites for a full-thickness abdominal wall defect repair was systematically investigated by a series of tests including wall thickness measurement, muscle regeneration analysis and angiogenesis assessment. The main findings were: (1) real-time and noninvasive degradation monitoring of the ECMB composites until complete degradation could be realized by chemical conjugation with a Cy5.5 NHS ester. (2) In a full-thickness abdominal wall defect model, the explant thickness could be used as an intuitional indicator for evaluating the tissue remodeling efficiency in the ECMB composites, and the accuracy of this indicator was verified by various examinations including collagen deposition, angiogenesis, and muscle regeneration. The present study could provide new insight into evaluating tissue repair effectiveness of the ECMB composites.
研究领域:
生物医学工程
·
组织工程
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 41
·2105614
·
IF 18.808
Xiangyi Yin,Yuanping Hao,Yun Lu,Dongjie Zhang,Yaodong Zhao,Li Mei,Kunyan Sui,Qihui Zhou,Jilin Hu
Developing bio-multifunctional patches with natural extracellular matrix-like structures, excellent high adhesion in the wet state, self-healing ability, antibacterial activity, and favorable cell responses for accelerating tissue healing is highly desirable in clinical applications. Herein, bio-multifunctional composite hydrogels are developed by coupling carboxymethyl chitosan and 4-arm poly (ethylene glycol) aldehyde for full-thickness abdominal wall defect repair. The prepared hydrogels exhibit excellent self-healing and mechanical properties, high adhesion in the wet state, and significant antibacterial ability. In vitro cellular experiments show that the hydrogels combined with recombinant bovine basic fibroblast growth factor remarkably promote cell proliferation and then accelerate full-thickness abdominal wall defect repair in a rat model. The histomorphological evaluation shows that compared to the commercial polypropylene mesh used clinically, the designed hydrogel patches facilitate an increase in the thickness and integrity of the abdominal wall tissue by upregulating the production of Ki67, enhancing the formation of collagen, inducing neovascularization, and inhibiting inflammation by reducing the expression of IL-6, TNF-α, and IL-1β. The results demonstrate that this novel bio-multifunctional hydrogel patch holds great potential for the treatment of full-thickness abdominal wall defects.
研究领域:
生物材料
·
组织工程
·
再生医学
·
生物医学工程
·
伤口愈合
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）