VIROLOGY
·
2025
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IF 2.4
Yao Yao,Rui Jing,Xiang Liu,Li Kang,Pinghuang Liu
Feline infectious peritonitis (FIP), a fatal disease in cats caused by feline infectious peritonitis virus (FIPV), has limited treatment options and lacks effective vaccines. Cepharanthine (CEP), a natural isoquinoline alkaloid, possesses many medicinal properties, including antiviral activities. However, the role of CEP in management of FIPV infection remains poorly understood. Here, we identified that CEP had a potent ability to inhibit FIPV infection in vitro . CEP significantly inhibited FIPV infection when administered at different times, with co-treatment showing the most significant inhibitory effect. Time-of-addition assays demonstrated that CEP exerted antiviral activity during the post-entry stages of the FIPV infection. We also verified that CEP inhibited FIPV infection not through affecting type I interferon expression, and it could decrease pro-inflammatory factors expression induced by FIPV infection. The combination of CEP and GS-441524 exhibited synergistic antiviral effects against FIPV infection. Our findings highlight the therapeutic potential of CEP for treatment of FIP.
研究领域:
兽医学
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病毒学
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药理学
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DOI
PubMed
高影响因子
Collagen and Leather
·
2023
·Vol. 5
·期 1
·1-13
·
IF 0
Dong Jinhong,Wang Tianyou,Li Haotian,Zhang Jianhua,Zhang Hengjie,Liu Weijie,You Xinru,Gu Zhipeng,Li Yiwen,Chen Xianchun,Fu Qiang
The development of advanced sustainable biomedical materials with superior biosafety and bioactivity for clinical applications is highly desirable. In the present investigation, biomass-based nanoparticles (NPs) were assembled through the Mannich reaction between the plant polyphenols and the broad-spectrum antibiotic tigecycline (TG). The fabricated NPs with uniform size demonstrated excellent oxidative balance effects, pH-responsive release properties and antibacterial performances. Furthermore, the intracellular and in vivo studies confirmed that the NPs are capable of reducing oxidative damage to cells and significantly repairing tissue injury in mice with peritonitis. This work presents an effective method and idea for constructing biomass-based materials for the treatment of infection-induced diseases. Graphical Abstract
研究领域:
生物医学材料
·
药理学
·
微生物学
✓ 已验证
DOI
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Maojuan Liu,Yunhe Li,Yang Song,Erqun Song
Peritoneal dialysis-associated peritonitis (PDAP), primarily caused by bacterial infections, is a severe complication and a major contributor to mortality in peritoneal dialysis patients. Early detection of bacterial infections and prompt therapeutic interventions are critical for effective PDAP management. Herein, we report the development of a novel apoptotic body (AB)-hitchhiked manganese-based T1-weighted magnetic resonance imaging (MRI) probe (PMA) for noninvasive and in situ bacterial detection in the abdominal cavity, offering a potential early warning system for PDAP. When administered to PDAP model mice, the PMA probes selectively accumulated in bacteria-infected regions via AB-mediated macrophage targeting. Subsequently, the PMA probes disintegrated in the acidic microenvironment of bacterial infection, which resulted in the MRI signal ON, therefore imaging the bacterial infection in vivo accurately. Remarkably, due to the protein-assisted T1-weighted signal amplification, the PMA probe-based MRI strategy detected bacterial loads in the abdominal cavity approximately 10 times lower than those identified by biochemical analysis for PDAP diagnosis. This approach achieves sensitive bacterial detection, holding great potential for the early diagnosis of PDAP in clinical practice in the future.
研究领域:
微生物学
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影像医学
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生物材料
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转化医学
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肾脏病学
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分子诊断
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纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2023
·
IF 6.1
Zhongcai Wu,Xuezhi Zuo,Xiuru Wang,Mengxia Shi,Han Zhu,Chujin Cao,Xiaoqin Liu,Wangqun Liang,Ying Yao,Le Wang
Peritoneal fibrosis is a complication of long-term peritoneal dialysis (PD) that restricts its clinical application for the treatment of end-stage renal disease. Lactobacillus casei Zhang (LCZ), a probiotic strain isolated from traditional fermented koumiss, exhibits health benefits such as anti-inflammatory and antioxidative effects, improvement of insulin resistance and mitigation of renal injury. However, whether LCZ can prevent peritoneal fibrosis remains unknown. Here, we assessed the effects of LCZ in a mouse model of PD-induced peritoneal fibrosis. Our results showed that the administration of LCZ significantly ameliorated peritoneal fibrosis in experimental mice. Macrophage infiltration, inflammatory M1 polarization and inflammatory cytokines in peritoneal dialysis effluents were effectively reduced by LCZ. Meanwhile, LCZ corrected gut dysbiosis and enriched beneficial bacteria that produce short-chain fatty acids, specifically Dubosiella, Lachnospiraceae, Parvibacter, and Butyricicoccus. Correspondingly, the local butyrate level in peritoneal dialysis effluents was significantly elevated by LCZ. Mechanistically, we found activation of PPARγ and inhibition of the NF-κB pathway in LCZ-treated mice, an observation that was replicated in a butyrate-treated macrophage cell line. In conclusion, our study suggests that LCZ is beneficial for preventing PD-induced peritoneal fibrosis through modulating the gut microbiota, enhancing butyrate production, activating PPARγ, and suppressing NF-κB-mediated inflammation.
研究领域:
微生物学
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免疫学
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肾病学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2025
·
IF 12.9
Zhi-Guo Wang,Ming Cai,Xue Xiao,Run-Lin Sun,Xi Liao,Rui Hong,Ju-Xiang Gou,Ka Li,Jia-Zhuang Xu,Zhong-Ming Li
Drainage tube (DT) intubation is frequently accompanied by distressing and even life-threatening complications. Herein, we engineer a peritoneum-inspired adaptive hydrogel sheath orchestrating long-term lubrication and broad-spectrum antibacterial properties onto the commercially-available DT surface for safe medical intervention. A thin conformal hydrogel sheath is formed by constructing the semi-interpenetrating hydrogel network to serve as the hydration layer, mimicking the lubricative peritoneal fluid layer of the peritoneum. The exposed protonated amino group of the semi-interpenetrating hydrogel network imparts the hydrogel sheath with an intrinsic antibacterial capacity, imitating the peritoneal inherent immunoregulatory function that resists bacterial invasion. The resultant hydrogel sheath exhibits long-term lubricity, prominent broad-spectrum antibacterial property, remarkable robustness in various harsh environments, and excellent antifouling property without comprising biocompatibility. As demonstrated by an in vivo rabbit model of peritoneal drainage, the application of on DT not only alleviates skin tissue trauma and inflammation effectively, but also inhibits bacterial invasion to avoid potential infection. The current work opens a valuable avenue to develop the functional DT to meet clinical needs.
研究领域:
生物医学工程
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材料科学
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医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Bio
·
2024
·
IF 8.7
Zequn Zhang,Chao Yin,Xianwen Song,Xi Liu,Chonglei Zhong,Jun Zheng,Yaqiong Ni,Rujuan Shen,Yihang Guo,Xiaorong Li,Changwei Lin,Yi Zhang,Gui Hu
Peritoneal adhesions commonly occur following abdominal or pelvic surgery and can cause serious complications. Currently, physical barriers are the primary approach used in clinical practice to prevent adhesion, although their effectiveness is frequently inadequate. In this study, we developed an injectable peptide-loaded hydrogel with multiple functions, including self-fusion, tissue-adhesiveness, anti-inflammation, anti-cell adhesion and anti-angiogenesis. To assess the effectiveness of these hydrogels, which are stabilized by dynamic imine bonds and acetal connections, in preventing postoperative abdominal adhesions, we utilized both a rat abdominal adhesion model and a rat model simulating repeated-injury adhesions. In comparison to the commercially available HA hydrogel, as-prepared hydrogels exhibited significant reductions in inflammation, fibrosis, and angiogenesis, leading to an obvious decrease in peritoneal adhesions. Moreover, this peptide-loaded hydrogel demonstrated an ideal degradation time, maintaining an in vivo viability for about 10 days. We believe this peptide-loaded hydrogel presents a promising solution for the challenging clinical issue of postoperative abdominal adhesions.
研究领域:
生物医学工程
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组织工程
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药剂学
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外科手术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Canadian Journal of Infectious Diseases & Medical Microbiology
·
2025
·Vol. 2025
·期 1
·5553355
·
IF 2.6
Kai Ming Chow,Siu Kwan Wo,Simon Wai Yin So,Phyllis Mei Shan Cheng,Keary Rui Zhou,Wai Li Lim,Joan Zhong Zuo,Philip Kam Tao Li
Background: With emerging antibiotic resistance, many patients on peritoneal dialysis require newer antibiotic treatment such as daptomycin. Inadequate clinical information exists across different peritoneal dialysis solutions, including icodextrin, for the stability of intraperitoneal daptomycin. To guide the clinical practice of intraperitoneal daptomycin treatment, we need to establish the stability of daptomycin at dextrose concentration higher than 1.5% and icodextrin, as well as the duration of stability. Methods: We tested the stability of daptomycin in three types of peritoneal dialysis bags (UltraBag dextrose 2.5%, UltraBag icodextrin 7.5%, and Stay-Safe Balance 2.3%). Daptomycin was reconstituted with water for injection (50 mg/mL), followed by administration to peritoneal dialysis bags to obtain the final daptomycin concentrations of 70 μg/mL (equivalent to 140 mg/2L, the maintenance level) and 245 μg/mL (equivalent to 490 mg/2L, the loading level). The bags were then placed at ambient temperature (25°C) followed by withdrawing 5 mL samples at 0, 4, 8, 12, 24, and 48 h for UltraBag dextrose 2.5% and UltraBag icodextrin 7.5% and 0, 4, 8, 12, and 24 h for Stay-Safe Balance 2.3%. The concentrations of daptomycin in the collected samples were quantified by high-performance liquid chromatography with diode array detector (HPLC-DAD). Results: Under ambient condition, daptomycin was stable at maintenance level in UltraBag dextrose 2.5% for 48 h and in UltraBag icodextrin 7.5% or Stay-Safe Balance 2.3% for 24 h. For loading level, daptomycin was stable in UltraBag dextrose 2.5% and Stay-Safe Balance 2.3% for 12 h and in UltraBag icodextrin 7.5% for 48 h. Conclusions: Current stability results support and guide the use of intraperitoneal daptomycin in different dialysis solutions. Patients with peritonitis requiring icodextrin exchange and assisted preparation of daptomycin can benefit from nurses who provide daily home visit based on our stability results.
研究领域:
药理学
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临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6.8
Jiaxian Cai,Chen Lian,Zeyu Lu,Qian Shang,Li Wang,Zhihao Han,Yueqing Gu
Hepatocellular carcinoma (HCC) is a frequent malignancy that has a high death rate and a high rate of recurrence following surgery, owing to insufficient surgical resection. Furthermore, HCC is prone to peritoneal metastasis (HCC-PM), resulting in a significant number of tiny cancer lesions, making surgical removal more challenging. As a potential imaging target, FGFR4 is highly expressed in tumors, especially in HCC, but is less expressed in the normal liver. In this study, we used computational simulation approaches to develop peptide I0 derived from FGF19, a particular ligand of FGFR4, and labeled it with the NIRF dye, MPA, for HCC detection. In surgical navigation, the TBR was 9.31 ± 1.36 and 8.57 ± 1.15 in HepG2 in situ tumor and HCC-PM models, respectively, indicating considerable tumor uptake. As a result, peptide I0 is an excellent clinical diagnostic reagent for HCC, as well as a tool for surgically resecting HCC peritoneal metastases.
研究领域:
肿瘤学
·
医学影像学
·
外科肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
CLINICA CHIMICA ACTA
·
2024
·
IF 3.2
Ting Yang,Bangbang Wei,Jing Liu,Xinxin Si,Lulu Wang,Chunming Jiang
Background Peritoneal dialysis (PD) helps prevent lethal complications of end-stage renal disease (ESRD). However, the clinical outcomes are affected by PD-related complications. We investigated metabolic biomarkers to estimate the clinical outcomes of PD and identify patients at high risk of downstream complications and recurrent/relapsing infections. Methods Metabolites of normal control and ESRD patient were compared via an untargeted metabolomic analysis. Potential metabolic biomarkers were selected and quantified using a multiple reaction monitoring-based target metabolite detection method. A nomogram was built to predict the clinical outcomes of PD patients using clinical features and potential metabolic biomarkers with the least absolute shrinkage and selection operator Cox regression model. Results Twenty-five endogenous metabolites were identified and analyzed. ESRD-poor clinical outcome-related metabolic modules were constructed. Adenine, isoleucine, tyramine, xanthosine, phenylacetyl-L-glutamine, and cholic acid were investigated using the weighted gene correlation network analysis blue module. Potential metabolic biomarkers were differentially expressed between the NC and ESRD groups and the poor and good clinical outcomes of PD groups. A 3-metabolite fingerprint classifier of isoleucine, cholic acid, and adenine was included in a nomogram predicting the clinical outcomes of PD. Conclusion Metabolic variations can predict the clinical outcomes of PD in ESRD patients.
研究领域:
医学
·
代谢组学
·
肾脏病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Metabolomics
·
2022
·Vol. 18
·期 11
·1-12
·
IF 4.747
Yang Bo,Yin Hongqing,Wang Jianwei,Gan Jiali,Li Jingfang,Han Rui,Pei Ming,Song Lili,Yang Hongtao
Background Restless legs syndrome (RLS) is a neuromotor disorder, and dialysis patients are more likely to develop RLS. RLS often causes sleep disorders, anxiety and depression in patients. It will increase the risk of death and severely affect the life of patients. At present, RLS has not received enough recognition and attention, and the misdiagnosis rate can reach more than 10%. Methods The discovery set selected 30 peritoneal dialysis (PD) patients and 27 peritoneal dialysis patients with RLS (PD-RLS). A metabolomics method based on ultra performance liquid chromatography tandem quadrupole time-of-flight mass spectrometric method (UPLC-Q-TOF/MS) was used to analyze the differential metabolites of the two groups. 51 PD patients and 51 PD-RLS patients were included in the validation set. The receiver operating characteristic (ROC) analysis was used to evaluate the early diagnostic biomarkers, and the correlation between the differential metabolites and laboratory test indexes was analyzed to explore the biological function of the differential metabolites. Results Through the integrated analysis, four metabolites can be used as markers for the diagnosis of PD-RLS, including Hippuric acid, Phenylacetylglutamine, N,N,N-Trimethyl-L-alanyl-L-proline betaine and Threonic acid. Through ROC analysis, it is found that they can be used as a metabolic biomarker panel, and the area under the curve of this combination is more than 0.9, indicating that the panel has good diagnostic and predictive ability. Conclusion Metabolomics based on UPLC-Q-TOF/MS technology can effectively identify the potential biomarkers, and provide a theoretical basis for the early diagnosis, prevention and treatment on PD-RLS.
研究领域:
医学
·
代谢组学
✓ 已验证
DOI
PubMed
高影响因子
Science Bulletin
·
2025
·
IF 21.1
Qiang Wang,Yingfei Wen,Bo Bi,Kuan Li,Yuanqi Liu,Binbin Li,Shangbo Zhou,Zihang Li,Jiaqi Xu,Miaojuan Qiu,You Li,Meiying Wu,Yu Chen,Wei Wu,Jing Zhao
Peritoneal metastasis (PM) is typically intractable by immunotherapy due to an immunosuppressive microenvironment and the peritoneal-plasma barrier. Sonodynamic therapy (SDT) presents unique advantages of noninvasive in situ treatment and the potential for antitumor immune activation. Building upon SDT technology, the study reports on a novel biodegradable sonosensitizer, CaS 2 O 8 , characterized by a narrow bandgap, abundant oxygen vacancies and a rapid ultrasound (US) response for abdominal SDT. Such sonosensitizer only produces lethal reactive oxygen species (ROS) after US irradiation, which is nontoxic in a physiological environment. After US irradiation, CaS 2 O 8 yields a large amount of sulfate radical (•SO 4 - ), as well as sonodynamic related ROS (•OH, and 1 O 2 ), which exerts a synergistic effect with Ca 2+ overload to induce Z-conformation nucleic acid by augmenting oxidative damage. As a result, the PANoptosis is initiated through the ZBP1/RIPK3 pathway in tumor cells. This inflammatory cell death leads to a multi-faceted release of tumor cell contents which serve as an in situ tumor antigen to induce a robust antitumor immune response. Notably, the precision sono-immunotherapy enhances the infiltration of T cells into tumors by transforming an immunosuppressive phenotype into an immunostimulatory one. Therefore, targeting PANoptosis by CaS 2 O 8 -induced SDT can provide an alternative or additional clinical treatment and prolonged survival outcome for patients with PM.
研究领域:
肿瘤学
·
生物医学工程
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2016
·Vol. 17
·期 4
·1600262
·
IF 3.68
Qin Yun,Si Song Wang,Shan Xu,Jin Ping Yang,Juan Fan,Ling Lin Yang,Yue Chen,Shao Zhi Fu,Jing Bo Wu
Colorectal peritoneal carcinomatosis (CRPC) is a common systemic metastasis of intra-abdominal cancers. Intraperitoneal chemotherapy against CRPC is at present the preferred treatment. The aim of this study is to develop a novel hydrogel drug delivery system through the combination of 5-fluorouracil (5-FU) loaded polymeric micelles and cisplatin (DDP) in biodegradable thermosensitive chitosan (CS) hydrogel. The prepared CS hydrogel drug is a free-flowing solution at room temperature and forms a stationary gel at body temperature. Therefore, a CRPC mouse model is established to investigate the antitumor activity of CS hydrogel drug system. The results suggest that intraperitoneal administration of CS hydrogel drug can inhibit tumor growth and metastasis, and prolong survival time compared with other groups, thus improving the chemotherapeutic effect. Ki-67 immunohistochemical analysis reveals that tumors in the CS hydrogel drug group has lower cell proliferation in contrast to other groups ( P < 0.001). Furthermore, hematoxylin-eosin staining of liver and lung tissue indicates that the CS hydrogel drug has also a certain inhibitory effect on colorectal cancer metastasis to the liver and lung. Hence, the work highlights the potential clinical applications of the CS hydrogel drug.
研究领域:
肿瘤学
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
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