FOOD CHEMISTRY
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2020
·
IF 6.306
Jia-Nan Yan,Yu-Qiao Wang,Xin-Yu Jiang,Jia-Run Han,Yi-Nan Du,Jin-Feng Pan,Hai-Tao Wu
The electrostatic complex coacervation between scallop Patinopecten. yessoensis male gonad hydrolysates (SMGHs) and κ -carrageenan ( κ -C) were monitored by using turbidimetry at various pH (1–12) and biopolymer mixing ratio (9:1–1:9). The pH c exhibited ratio-independent behavior, and pH φ1 , pH max exhibited ratio-dependent behavior, respectively. The decreasing ratio enhanced the gel strength of SMGHs/ κ -C at higher pH while inversely at lower pH, ascribing to more SMGHs aggregates and stronger neutralization between positively charged patches in SMGHs and κ -C at lower pH and higher ratio. Moreover, SMGHs/ κ -C gel at acid condition exhibited lower relaxation times ( T 21 and T 23 ). Furthermore, the rheological and relaxation time T 2 data were well associated with microscopy images which indicated that SMGHs/ κ -C gel showed a well-distributed network structure at more acidic domains, supporting stronger gel rigidity and water-holding capacity.
研究领域:
食品科学
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 3
·1740
·
IF 4.9
Yonglin Ruan,Xuehui Li,Gang Zhai,Qiyong Lou,Xia Jin,Jiangyan He,Zhan Yin
The mechanism of fish gonadal sex differentiation is complex and regulated by multiple factors. It has been widely known that proper steroidogenesis in Leydig cells and sex-related genes in Sertoli cells play important roles in gonadal sex differentiation. In teleosts, the precise interaction of these signals during the sexual fate determination remains elusive, especially their effect on the bi-potential gonad during the critical stage of sexual fate determination. Recently, all-testis phenotypes have been observed in thecyp17a1-deficient zebrafish and common carp, as well as incyp19a1a-deficient zebrafish. By matingcyp17a1-deficient fish with transgenic zebrafishTg(piwil1:EGFP-nanos3UTR), germ cells in the gonads were labelled with enhanced green fluorescent protein (EGFP). We classified thecyp17a1-deficient zebrafish and their control siblings into primordial germ cell (PGC)-rich and -less groups according to the fluorescence area of the EGFP labelling. Intriguingly, the EGFP-labelled bi-potential gonads incyp17a1+/+ fish from the PGC-rich group were significantly larger than those of thecyp17a1−/− fish at 23 days post-fertilization (dpf). Based on the transcriptome analysis, we observed that thecyp17a1-deficient fish of the PGC-rich group displayed a significantly upregulated expression ofamhandgsdfcompared to that of control fish. Likewise, the upregulated expressions ofamhandgsdfwere observed incyp19a1a-deficient fish as examined at 23 dpf. This upregulation ofamhandgsdfcould be repressed by treatment with an exogenous supplement of estradiol. Moreover, tamoxifen, an effective antagonist of both estrogen receptor α and β (ERα and Erβ), upregulates the expression ofamhandgsdfin wild-type (WT) fish. Using thecyp17a1- andcyp19a1a-deficient zebrafish, we provide evidence to show that the upregulated expression ofamhandgsdfdue to the compromised estrogen signaling probably determines their sexual fate towards testis differentiation. Collectively, our data suggest that estrogen signaling inhibits the expression ofamhandgsdfduring the critical time of sexual fate determination, which may broaden the scope of sex steroid hormones in regulating gonadal sex differentiation in fish.
研究领域:
发育生物学
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内分泌学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
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2022
·Vol. 46
·期 12
·e17177
·
IF 2.609
Xue-Chen Wang,Xiao-Fan Cui,Yi-Nan Du,Shi-Qi Xu,Yu-Qiao Wang,Hai-Tao Wu
The gel properties and microstructure of scallop male gonad hydrolysates (SMGHs) were investigated by combing with κ-carrageenan (κ-C) and locust bean gum (LBG). It was shown that the initial elastic module of SMGHs/κ-C 0.1 Hz got marked improvement from 202.3 Pa to 326.7 Pa and the melting temperature also increased 6°C with the addition of LBG. According to the data of low-field nuclear magnetic resonance, the relaxation time T 23 markedly reduced in SMGHs/κ-C/LBG, representing the migration of water might result in a strong bind among SMGHs, κ-C, and LBG. Fourier transform infrared spectroscopy showed the existence of hydrogen bonding between LBG and SMGHs/κ-C. Low-temperature scanning electron microscopy showed that the SMGHs/κ-C/LBG ternary complexes possessed smaller voids and more dense network structures. These results could provide more possibilities for the application of SMGHs and polysaccharide complexes in functional hydrogel development. Novelty impact statement We evaluated the gel and microstructure properties of ternary composite gel consisted of protein hydrolysates from male gonad of scallop ( Patinopecten yessoensis ) (SMGHs), κ-carrageenan (κ-C), and locust bean gum (LBG). The results showed that the addition of κ-C and LBG significantly improved the gel properties of SMGHs.
研究领域:
食品科学
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材料科学
·
生物物理学
✓ 已验证
DOI
高影响因子
Ocular Surface
·
2024
·
IF 5.9
Xiaozhao Zhang,Guangjin Chen,Yan He,Qingming Tang,Ying Yin,Ying Jie
Secretory glands, responsible for tears and saliva production, play essential roles in maintaining ocular and oral well-being. Disruptions in gland secretion can arise from various factors, including rhythm disturbances associated with sleep disorders. However, the underlying mechanisms governing these disruptions remain largely unexplored. We demonstrate that BMAL1, a core component of the circadian system, plays a critical role in regulating secretory gland secretion. Loss of BMAL1 induces vacuolation and atrophy phenotypes in acinar cells, subsequently leading to cell apoptosis and gland hypofunction, but does not cause Sjogren's syndrome, which is characterized by localized inflammatory cell infiltration. Mechanically, BMAL1 directly modulates the transcription of ITPR2 and ITPR3, thereby altering the secretion of Lactoferrin and Lysozyme. Restoration of ITPR2 and ITPR3 expression in Bmal1 -deficient rats effectively alleviated the symptoms of lacrimal and parotid glands secretory dysfunction and significantly reduced dry mouth and dry eye conditions in rhythm-disordered rats. These findings highlight the essential role of BMAL1 in regulating salivary and lacrimal gland secretion and suggest a novel therapeutic approach for treating dry mouth and dry eyes associated with rhythm disorders.
研究领域:
昼夜生物学
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分泌生理学
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分子生物学
·
治疗学
✓ 已验证
DOI
高影响因子
Journal of Marine Science and Engineering
·
2021
·Vol. 9
·期 6
·677
·
IF 2.458
Kangxuan Chen,Shihao Li,Jianhai Xiang,Amir Sagi,Fuhua Li
The insulin-like androgenic gland hormone (IAG) plays a key role in male sexual differentiation and spermatogenesis in crustaceans. The expression of IAG is usually negatively regulated by neuropeptide hormones through the “eyestalk-AG” endocrine axis. However, the underlying mechanism is still largely unknown. In the present study, we performed a comparative transcriptome analysis on the androgenic gland (AG), the main secretory organ of IAG, ofL. vannameibefore and after unilateral eyestalk ablation. A total of 67 differentially expressed genes (DEGs) were identified, including some putative genes involved in sexual development. Interestingly, several genes related to molting and endocrine processes were found differentially expressed between the two treatments. Further investigation on the expression profiles of these genes andLv-IAGshowed that their expression patterns were closely correlated throughout the molting cycle. In addition, injection of 20-hydroxyecdysone (20E) could inhibit the expression ofLv-IAGat different molting stages in a dosage-dependent manner. The data indicated that ecdysteroids played important roles in regulating theLv-IAGexpression and might bridge the endocrine axis between eyestalk and AG. The present study provided new insights into understanding the regulation of sexual development in male crustacean.Keywords:insulin-like androgenic gland hormone;sexual development;endocrine axis;ecdysteroids;crustacean
研究领域:
内分泌学
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分子生物学
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发育生物学
·
水产养殖
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Sisi Zhang,Erna Jia,Weiqi Zhang,Zhiying Wang,Da Deng,Yixin Zhang,Xuefeng Huang,Qingquan Tian,Ying Tan,Bujun Wang,Feng Lin
The development of novel therapeutic approaches to facilitate endometrial repair and regeneration while preventing adhesion recurrence is a crucial research objective aimed at enhancing clinical outcomes for women with intrauterine adhesions (IUA). In this study, we introduced an injectable Alg-GMA/PTSB zwitterionic hydrogel, characterized by excellent biocompatibility, anti-protein adsorption properties, and biodegradability. In a rat model, the hydrogel significantly promoted the regeneration and angiogenesis of damaged endometrial tissue, leading to improved recovery of epithelial cells, glands, proliferation, and vascularization. Furthermore, it exhibited the ability to suppress cellular apoptosis and collagen deposition, thereby mitigating fibrosis. Additionally, the hydrogel restored the expression of estrogen/progesterone receptors and endometrial receptivity markers, contributing to enhanced embryo implantation and fertility. These findings underscore the potential of the hydrogel as a promising therapeutic strategy for addressing endometrial injury, reducing fibrosis, restoring fertility, and ultimately improving outcomes for women with IUA.
研究领域:
妇科学
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生殖医学
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生物材料
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组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）