Protocol for evaluating S-acylated protein membrane affinity using protein-lipid conjugates
Summary S -acylation of proteins allows their association with membranes. Here, we present a protocol for establishing a platform for membrane affinity evaluation of S -acylated proteins in vitro . We describe steps for preparing lipid-maleimide compounds, mCherry-p62 recombinant proteins, and total cellular membranes. We then detail procedures for synthesizing protein-lipid conjugates using lipid-maleimide compounds and recombinant proteins and evaluating the membrane affinity of protein-lipid conjugates. For complete details on the use and execution of this protocol, please refer to Huang Xue et al. 1
研究领域:
生物化学
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分子生物学
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细胞生物学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）