ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Nanhang Zhu,Yue Yu,Di An,Yating Zeng,Ke Kang,Qiangying Yi,Yao Wu
Focused on the newly secreted tumorous exosomes during melanoma immunotherapy, this work has pioneered an ultra-sensitive spatiotemporal-specific exosome detection strategy, leveraging advanced exosomal membrane engineering techniques. The proposed strategy harnesses the power of amplified lanthanide luminescence signals on these exosomes, enabling precise and real-time monitoring of the efficacy of melanoma immunotherapy. The methodology comprises two pivotal steps. Initially, Ac4ManNAz-associated metabolic labeling is employed to evolve azide groups onto the membranes of newly secreted exosomes with remarkable selectivity. These azide groups serve as versatile clickable artificial tags, enabling the precise identification of melanoma exosomes emerging during immunotherapy. Subsequently, lanthanide-nanoparticle-functionalized polymer chains are controllably grafted onto the exosome surfaces through click chemistry and in situ Fenton–RAFT polymerization, serving as robust signal amplifiers. When integrated with time-resolved fluorescence detection, this strategy yields detection signals with an exceptionally high signal-to-noise ratio, enabling ultra-sensitive detection of PD-L1 antigen expression levels on the spatiotemporal-specific exosomes. The detection strategy boasts a wide linear concentration range spanning from 1.7 × 104 to 1.7 × 109 particles/mL, with a remarkable theoretical detection limit of 1.28 × 103 particles/mL. The remarkable enhancements in detection sensitivity and accuracy facilitate the evaluation of the efficacy of immunotherapeutic interventions in the mouse B16 melanoma model, notably revealing a substantial disparity in PD-L1 levels between immunotherapy-treated and untreated groups (P < 0.01) and further emphasizing the cumulative therapeutic effect that intensifies with repeated treatments (P < 0.001).
研究领域:
肿瘤学
·
生物医学工程
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Zhuqing Huang,Xingtao Qi,Hai Zhang,Liequan Liu,Ze Zhang,Zhenyu Yang,Junchao Wei
Lithium metal batteries (LMBs) face critical challenges due to uncontrolled lithium dendrite growth and inhomogeneous Li+ flux, largely attributed to conventional separators’ poor interfacial compatibility. To address this, we propose a hydrogen bond-driven layer-by-layer (LbL) assembly strategy for engineering functional separators using poly(vinyl alcohol) (PVA) and tannic acid (TA). The optimized PP/(TA/PVA)15 separator leverages the synergistic interplay between PVA’s hydroxyl groups and TA’s carbonyl moieties, forming a robust hydrogen-bonded network that simultaneously enhances lithiophilicity, regulates Li+ flux uniformity, and immobilizes anions. The interfacial design achieves exceptional electrochemical performance: Li//Li symmetric cells maintain stable operation for 800 h at 0.5 mA cm–2/0.5 mAh cm–2, while Li//LiFePO4 half cells retain 73.8% capacity after 1000 cycles at 5C (decay rate: 0.026% per cycle). The separator further exhibits high ionic conductivity (0.94 mS cm–1) and Li+ transference number (0.63), outperforming conventional polyolefin counterparts. By integrating simplicity, scalability, and eco-friendliness, this work pioneers a universal interface chemistry paradigm for next-generation LMBs, offering transformative insights into separator engineering through molecular-level hydrogen-bonding control.
研究领域:
材料科学
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电化学
·
能源存储
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Jiang Xiao,Zhongquan Song,Tengfei Liu,Zengchao Guo,Xiaohui Liu,Hui Jiang,Xuemei Wang
Drug-resistant bacterial infections and their lipopolysaccharide-related inflammatory complications continue to pose significant challenges in traditional treatments. Inspired by the rapid initiation of resident macrophages to form aggregates for efficient antibacterial action, this study proposes a multifunctional and enhanced antibacterial strategy through the construction of novel biomimetic cell membrane polypeptide nanonets (R-DPB-TA-Ce). The design involves the fusion of end-terminal lipidated polypeptides containing side-chain cationic boronic acid groups (DNPLBA) with cell membrane intercalation engineering (R-DPB), followed by coordination with the tannic acid-cerium complex (TA-Ce) to assemble into a biomimetic nanonet through boronic acid-polyphenol-metal ion interactions. In addition to the ability of RAW 264.7 macrophages cell membrane components' (R) ability to neutralize lipopolysaccharide (LPS), R-DPB-TA-Ce demonstrated enhanced capture of bacteria and its LPS, leveraging nanoconfinement-enhanced multiple interactions based on the boronic acid–polyphenol nanonets skeleton combined with polysaccharide. Utilizing these advantages, indocyanine green (ICG) is further employed as a model drug for delivery, showcasing the exceptional treatment effect of R-DPB-TA-Ce as a new biomimetic assembled drug delivery system in antibacterial, anti-inflammatory, and wound healing promotion. Thus, this strategy of mimicking macrophage aggregates is anticipated to be further applicable to various types of cell membrane engineering for enhanced antibacterial treatment.
研究领域:
生物材料学
·
感染与免疫学
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药剂学
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2017
·Vol. 27
·期 12
·1604916
·
IF 12.124
Shi-Ying Li,Wen-Xiu Qiu,Hong Cheng,Fan Gao,Feng-Yi Cao,Xian-Zheng Zhang
In this paper, a plasma membrane engineering approach is reported for tumor targeting drug delivery and contact-cell-enhanced photodynamic therapy (“CONCEPT”) by anchoring functionalized conjugates to cell vehicles. The membrane anchoring conjugates are comprised of a positively charged tetra-arginine peptide sequence, a palmitic-acid-based membrane insertion moiety, and a lysine linker whose ε-amine is modified with camptothecin (CPT), protoporphyrin IX (PpIX), or fluorescein (FAM). The amphipathic CPT, PpIX, or FAM conjugates (short as aCPT, aPpIX, or aFAM, respectively) can easily and steadily anchor or coanchor on the cell membrane of RAW264.7 cells (short as RCs), red blood cells, or mesenchymal stem cells. After anchoring aPpIX in RC cells, the tumor targeting ability and therapeutic effect of aPpIX-anchored RC cells (short as aPRCs) is demonstrated in vitro and in vivo. Importantly, aPRCs exhibit the “CONCEPT” effect, which can enhance the therapeutic efficacy and reduce side effects at the single cell level. Due to the good tumor-targeting ability, aPRCs can efficiently inhibit the tumor growth with no systemic toxicity after photoirradiation by photodynamic therapy.
研究领域:
生物医学工程
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肿瘤学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2020
·Vol. 145
·期 10
·3626-3633
·
IF 3.978
Julan Xia,Sujuan Sun,Xianhua Wu,Yan Huang,Chunyang Lei,Zhou Nie
In a cellular microenvironment, numerous biomolecules are involved in various physiological and pathological processes. However, for the in-depth and comprehensive understanding of their roles at the molecular level, there is still a lack of detection techniques for the in situ tracking of these biomolecules in a local environment. Herein, we engineered a membrane insertion peptide (MIP) as an enzyme-activated membrane insertion peptide probe (eaMIP) that allowed the in situ tracking of the activity of target enzymes in living cells. In this strategy, the membrane insertion capacity of the MIP motif in each eaMIP was caged by appending a chemical moiety. In the presence of target enzymes, the caging moiety in each eaMIP was removed by enzymatic decaging, leading to the generation of active MIPs. The versatility of this design was demonstrated by lighting up different tumor cells with distinct fluorescence signal patterns, affording an alternative tool for clinical diagnostics, biochemical research and membrane engineering.
研究领域:
细胞生物学
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生物技术
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癌症研究
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Xiaoxin Wu,Si Chen,Huasen Shen,Yunan Tian,Guoning Wu,Tingting Ma,Mengjun Li,Yuyu Li,Zhaohuai Li
Separators constitute vital components in high-energy-density lithium metal batteries (LMBs), yet their propensity to fail under lithium (Li) dendrite growth remains a critical limitation. In this work, we report a commercial Celgard separator engineered with pearl-like nanospheres (Celgard@PNS) to enable electrochemically stable and dendrite-free lithium metal anodes (LMAs). The PNS-functionalized layer in the Celgard@PNS ensures rapid and homogeneous Li ion flux, which stabilizes LMAs and effectively mitigates dendritic Li deposition. Moreover, the PNS modification fosters the development of a robust solid-electrolyte interphase (SEI) layer. The Celgard@PNS-based Li||Cu half-cell exhibits stable Coulombic efficiency for 110 cycles, and the corresponding Li||Li symmetric cell sustains long cycling stability (>900 h) at a high current density of 10 mA cm −2 with an areal capacity of 1 mAh cm −2 . Impressively, when paired with a high-mass-loading NCM811 cathode (22.5 mg cm −2 ), the Li||NCM811 full cell retains 81.4 % of its initial capacity after 140 cycles at 0.5 C. These results underscore the potential of the Celgard@PNS separator for practical implementation in high-capacity LMBs.
研究领域:
材料科学
·
电化学
·
能源存储技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POWER SOURCES
·
2024
·
IF 8.1
Tiao Huang,Shenglin Wang,Jingyi Wu,Hui Hu,Jianyi Wang,Xiaosong Zhang,Yanan Gao
Aqueous zinc–iodine (Zn–I) batteries show a great development potential in the field of energy storage due to their advantages of low cost, high safety and ecological friendliness. However, Zn dendrite growth and shuttle effect of polyiodide seriously aggravate the self-discharge behavior and thus decrease the cycle life of batteries . Therefore, controlling the diffusion of zinc ion and inhibiting soluble polyiodide in the separator are the most effective strategy to extend the lifespan of batteries. Herein, we design and prepare a multifunctional Janus separator composed of a covalent organic framework (COF) layer, a commercial glass fiber (GF) substrate and a graphene (Gr) layer to achieve durable Zn–I batteries. The COF layer features abundant polar groups and uniform open-channel mesopores enabling homogeneous Zn 2+ flux and simultaneously inhibiting polyiodide species migration. The highly conductive Gr layer can not only load iodide but also effectively activate the redox kinetics of iodine species. The full cell assembled with the TTA-DHTPA-COF@GF@Gr Janus separator demonstrated a recorded energy density of up to 6.4 mA h·cm −2 at a high current density of 20 mA cm −2 in aqueous Zn–I batteries. The design of the multifunctional Janus separator provides a new insight into separator engineering for durable Zn-ion batteries.
研究领域:
材料科学
·
电化学
·
能源存储
✓ 已验证
DOI
高影响因子
ACS Nano
·
2016
·
IF 13.334
Jie Shen,Gongping Liu,Kang Huang,Zhenyu Chu,Wanqin Jin,Nanping Xu
Two-dimensional (2D) materials with atomic thickness and extraordinary physicochemical properties exhibit unique mass transport behaviors, enabling them as emerging nanobuilding blocks for separation membranes. Engineering 2D materials into membrane with subnanometer apertures for precise molecular sieving remains a great challenge. Here, we report rational-designing external forces to precisely manipulate nanoarchitecture of graphene oxide (GO)-assembled 2D channels with interlayer height of ∼0.4 nm for fast transporting and selective sieving gases. The external forces are synergistic to direct the GO nanosheets stacking so as to realize delicate size-tailoring of in-plane slit-like pores and plane-to-plane interlayer-galleries. The 2D channels endow GO membrane with excellent molecular-sieving characteristics that offer 2–3 orders of magnitude higher H2 permeability and 3-fold enhancement in H2/CO2 selectivity compared with commercial membranes. Formation mechanism of 2D channels is proposed on the basis of the driving forces, nanostructures, and transport behaviors.
研究领域:
材料科学
·
膜分离技术
·
纳米科技
·
化学工程
·
物理化学
·
气体传输
·
界面科学
✓ 已验证
DOI
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-11
·
IF 15.7
Hu Yajie,Li Puying,Lai Guobin,Lu Bing,Wang Haiyan,Cheng Huhu,Wu Mingmao,Liu Feng,Dang Zhi-Min,Qu Liangti
Line-filtering electrochemical capacitors (LFECs) are demonstrating advantages in line filtering over traditional electrolytic capacitors. However, they can only function at no-load or low-power conditions due to the limited high-frequency capacitance resulting from the excessive ionic resistance, despite much progress in electrode materials. Here, we show separators dominate both ion migration and capacitance in LFECs. A 3 μm-thick thread-anchor structured separator is developed, featuring both accelerated ionic transport and reliability, leading to a low ionic resistance of 25 mΩ cm 2 . With a phase angle of −80° at 120 Hz, the assembled device has an areal capacitance of 6.6 mF cm −2 . Furthermore, stack integration in parallel breaks the trade-off between capacitance and frequency response, boosting the areal capacitance by two orders of magnitude without decay of frequency characteristics. The On-board field test demonstrates that voltage ripples are steadily suppressed below 5% even for practical high-power line filtering with a load power density of 2.5 W cm −2 , three orders of magnitude higher than previous instances. This work opens up a perspective of separator engineering for the development of high-performance line-filtering electrochemical capacitors and promotes their applications in practical high-power scenarios.
研究领域:
电化学
·
能量存储
·
材料科学
·
电力电子
·
应用物理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Jieyuan Wu,Wei Chen,Yutong Zhang,Xuanxuan Zhang,Jian-Ming Jin,Shuang-Yan Tang
The biosynthetic efficiency of curcumin, a highly bioactive compound from the plant Curcuma longa, needs to be improved. In this study, we performed host cell and biosynthetic pathway engineering to improve curcumin biosynthesis. Using in vivo-directed evolution, the expression level of curcuminoid synthase (CUS), the rate-limiting enzyme in the curcumin biosynthetic pathway, was significantly improved. Furthermore, as curcumin is a highly hydrophobic compound, two cell membrane engineering strategies were applied to optimize the biosynthetic efficiency. Curcumin storage was increased by overexpression of monoglucosyldiacylglycerol synthase from Acholeplasma laidlawii, which optimized the cell membrane morphology. Furthermore, unsaturated fatty acid supplementation was used to enhance membrane fluidity, which greatly ameliorated the damaging effect of curcumin on the cell membrane. These two strategies enhanced curcumin biosynthesis and demonstrated an additive effect.
研究领域:
生物技术
·
代谢工程
·
微生物学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2023
·
IF 29.4
Fanyi Huang,Yawei Du,Yanjia Chen,Zeping Qiu,Zhiyan Wang,Yingze Fan,Yunjing Shi,Zhuojin Li,Ke Yang,Wenguo Cui,Wei Jin
Lesion core is the area with the most serious injury and vigorous repair. Existing nanocarriers are difficult to break through the targeted delivery to lesion core and the precise treatment for intracellular and extracellular microenvironment. Herein, a cellular membrane-engineered nanovesicle (CMEV) with hierarchical structure is constructed using double emulsion-extrusion method, by integrating neutrophil membrane, functional antibody and gelled drug-loaded core, as ‘three-stage booster’ to target the lesion core and deliver catestatin (CST), a small therapeutic peptide, for ischemic cardiomyopathy therapy. By coating neutrophil membrane outside the shell, CMEV is endowed with the function of neutrophil-like migration to achieve the first stage of tissue targeting. Based on the specific anchoring to injured myocardium, myosin light chain 3 (MLC3) antibody was embedded to fulfill the second stage of CMEV accumulation in lesion core. The gelled core containing CST-sodium alginate (NaAlg) with pH-responsive shell is prepared by ionic cross-linking to accomplish the third stage of precise CST administration. Triggered by the microenvironment, NaAlg electrostatically adheres to the lesion core for sustained release, enhancing the efficacy of CST in improving cardiomyocyte apoptosis, excessive fibrosis, macrophage polarization and angiogenesis. Thus, the “three-stage booster” nanovesicle significantly ameliorates cardiac function and adverse remodeling to treat ischemic cardiomyopathy. This article is protected by copyright. All rights reserved
研究领域:
纳米技术
·
药学
·
心脏病学
·
生物材料
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
Nature Communications
·
2018
·Vol. 9
·期 1
·1-7
·
IF 12.353
Ge Xiaolin,He Yubin,Liang Xian,Wu Liang,Zhu Yuan,Yang Zhengjin,Hu Min,Xu Tongwen
Synthetic polyelectrolytes, capable of fast transporting protons, represent a challenging target for membrane engineering in so many fields, for example, fuel cells, redox flow batteries, etc. Inspired by the fast advance in molecular machines, here we report a rotaxane based polymer entity assembled via host–guest interaction and prove that by exploiting the thermally triggered translational motion (although not in a controlled manner) of mechanically bonded rotaxane, exceptionally fast proton transfer can be fulfilled at an external thermal input. The relative motion of the sulfonated axle to the ring in rotaxane happens at ~60 °C in our cases and because of that a proton conductivity (indicating proton transfer rate) of 260.2 mS cm −1 , which is much higher than that in the state-of-the-art Nafion, is obtained at a relatively low ion-exchange capacity (representing the amount of proton transfer groups) of 0.73 mmol g −1 .
研究领域:
材料科学
·
化学工程
·
电化学
·
分子机器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）