Analytical Methods
·
2018
·Vol. 10
·期 16
·1833-1841
·
IF 2.073
Yun-Shang Yang,Chun-Mei Ma,Ying-Peng Zhang,Qing-Hua Xue,Jia-Xi Ru,Xiao-Yu Liu,Hui-Chen Guo
A fluorescence probe based on cinnamyl pyrazoline was developed and synthesized. The probe detected Zn2+ ion that was mixed with a series of cations in aqueous ethanol with high selectivity and high sensitivity. After adding Zn2+, the fluorescence intensity was significantly enhanced, with a 56-fold increase. The detection limits and binding constants of 2.95 × 10−8 M and 1.468 × 104 M−1 were calculated by a fluorescence titration experiment. The 1 : 1 binding chemometrics of the 1–Zn2+ complex was analyzed by Job's plot. The probe showed good reversibility when adding PPi. Additionally, the “turn-on” fluorescent probe with excellent membrane permeability was satisfactorily applied to microscopic imaging for the detection of Zn2+ in baby hamster kidney (BHK)-21 cells.
研究领域:
生物化学
·
分析化学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
CRYSTAL GROWTH & DESIGN
·
2016
·
IF 4.425
Xia-Lin Dai,Song Li,Jia-Mei Chen,Tong-Bu Lu
Pharmaceutical cocrystallization is proposed as a new method to enhance membrane permeability of a BCS class III model drug, 5-fluorouracil (5FU). Three cocrystals of 5FU, 5FU/3-hydroxybenzoic acid (1), 5FU/4-aminobenzoic acid (2), and 5FU/cinnamic acid (3), were successfully synthesized by a slurry method or a liquid-assisted grinding process. Spectroscopic methods, thermal analysis, and X-ray diffraction were used to characterize these new forms. The permeability was studied using a Franz diffusion cell and silicone membrane. All of the cocrystals showed improved membrane permeability compared to free 5FU. The cumulative amount per unit area of permeated 5FU in the first 10 h for 1–3 was increased by 41, 70, and 83%, and the steady penetration rates of 1–3 were increased by 38, 66, and 79%, respectively, as compared to the pure drug. Structure–permeability correlation study finds a link between intermolecular interactions and molecular packing in cocrystals and their permeability behavior and has important implications for use of a cocrystallization approach to improve drugs’ permeability in the pharmaceutical field.
研究领域:
药学
·
生物医学工程
·
药物化学
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Hafiz Abdul Rasheed,Abdur Rehman,Xiaochen Chen,Tariq Aziz,Fahad Al-Asmari,Majid Alhomrani,Abdulhakeem S. Alamri,Haiying Cui,Lin Lin
Listeria monocytogenes is a prominent cause of foodborne illnesses and public health concerns worldwide. Naturally active compounds have been extensively explored for their potential to reduce foodborne pathogenic contamination. This work is designed to study the chemical composition, antibacterial effect , and inhibition mechanism of Citrus bergamia essential oil (CB-EO) against L. monocytogenes . The CB-EO composition revealed the major components of the total account are d -limonene (23.2%), linalyl acetate (14.01%), and linalool (9.96%). The inhibitory effect was examined at different concentrations of CB-EO, and the MIC value was 1.5 mg/mL against L. monocytogenes . The CB-EO concentration of 1.5 mg/mL significantly disturbed the membrane permeability and fluidity, leading to leakage of intracellular protein and DNA constituents, as revealed by the BCA protein assay and UV–Vis spectrophotometric analysis, respectively. However, a decrease in concentration to 0.75 mg/mL indicated a significant decline in intracellular macromolecule leakage as well as membrane disruption. Moreover, SEM and CLSM observations provided extensive insight into the inhibition mechanism of CB-EO against L. monocytogenes . Finally, while investigating the anti-hemolysis activity, CB-EO and its major compounds, limonene , linalool, and linalyle acetate, have been found to be effective against the virulence agent listeriolysin O (LLO). Thus, it was proposed that CB-EO could act on L. monocytogenes cells through multiple mechanisms of action, with EO concentration providing a crucial role in determining the mode of action. This work offers deep insight into the inhibition mechanisms of natural substances, expanding the potential uses of CB-EO as a natural antimicrobial.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Zhen Tao,Chuanjun Tian,Chunyan Zhong,Bingjie Ji,Wenwen Li,Yongshan Zhao
As microbial resistance and recurrent bacterial infections escalate, the growing understanding of the interplay between antibiotic resistance and tolerance has sparked significant interest in the latter. Previous studies have demonstrated that the deletion of cation/proton antiporters (CPAs) induces bacterial phenotypes, such as slow growth and prolonged lag phases, which contribute to the development of tolerance. This study investigates the role of the NhaA protein in antibiotic tolerance in Escherichia coli using CRISPR/Cas9 gene editing to delete the NhaA protein. Our results suggest that the NhaA protein plays a key role in modulating antibiotic tolerance. In response to NhaA deletion, E. coli adapts through multiple mechanisms, including changes in membrane permeability, enhanced efflux activity, increased membrane fluidity, disruption of the proton motive force (PMF), and a reduction in intracellular ATP levels. These adaptive changes collectively promote the development of antibiotic tolerance. Understanding these tolerance mechanisms could uncover new therapeutic targets, help prevent the emergence of tolerance, or sustain bacteria cells in a tolerant state, providing crucial strategies to combat the rise of antibiotic-resistant bacteria.
研究领域:
微生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2019
·
IF 0
Shunyu Xiang,Xiaozhou Ma,Huan Shi,Ting Ma,Cuiling Tian,Ya Chen,Haitao Chen,Xue Chen,Ke Luo,Lin Cai,Daibin Wang,Yang Xue,Jin Huang,Xianchao Sun
As a kind of promising nanopesticide, in contrast to traditional synthesis strategies, the application of a polysaccharide in silver nanoparticle preparation can improve its stability and avoid the usage of harmful substances. In this work, a two-step strategy for synthesizing silver nanoparticles (A-AgNPs) from aldehyde-modified sodium alginate (ASA) was introduced. The size of the A-AgNPs synthesized can be controlled from 6 to 40 nm with a high dispersibility in water. Furthermore, compared to naked AgNPs (n-AgNPs), the A-AgNPs showed improved broad-spectrum antimicroorganism activity. We found that the A-AgNPs mainly exerted their antifungal activity through the changing of cell membrane permeability and affecting the soluble protein synthesis, destruction of DNA structure, and inhibition of DNA replication. Meanwhile, the A-AgNPs showed no inhibition of rice and N. benthamiana seed germination. Considering its high biocompatibility and the highly efficient antimicroorganism activity, A-AgNPs can be potentially applied in plant protection science research.
研究领域:
生物化学
·
纳米生物学
·
植物病理学
✓ 已验证
产品关联
DOI
PubMed
SEPARATION AND PURIFICATION TECHNOLOGY
·
2018
·
IF 3.927
Jia-Jie Lu,Yi-Hang Gu,Yan Chen,Xi Yan,Ya-Jun Guo,Wan-Zhong Lang
Graphene oxide (GO) as a unique 2D material attracts much attention. It can be employed to prepare graphene-based membranes by vacuum filtration on microporous substrates. However, the permeability of graphene-based membranes is mainly limited by its interlamellar spacing. In this work, the interlamellar spacing of graphene-based membranes is expanded by grafting poly (ethylene imine) (PEI) on GO sheets. The novel graphene-based membranes present ultrahigh permeability without sacrificing the rejection for the filtration of dye wastewater. The original GO membrane (GOM) only has a pure water permeation flux (PWP) value of 27.0 L·m −2 ·h −1 bar −1 . However, the novel graphene-based membranes prepared with the modified GO by PEI-600 and PEI-10000 (recorded as PGOM-600 and PGOM-10000) markedly increase to 208.1 and 450.2 L·m −2 ·h −1 ·bar −1 respectively. In the separation of dye wastewater, GOM, PGOM-600 and PGOM-10000 exhibit much higher rejections for direct red and methyl blue. However, the modified graphene-based membranes (PGOM-600 and PGOM-10000) present more than 7–20 times higher permeation flux than that of original GOM without sacrificing dye rejection. Thus, this work provides graphene-based membranes with ultrahigh permeation flux for dye removal from wastewater.
研究领域:
材料科学
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 44
·20743-20750
·
IF 3.591
Huan-Huan Ding,Jia-Zhu Chigan,Jian-Bin Zhen,Lu Liu,Yin-Sui Xu,Cheng Chen,Ke-Wu Yang
It is imperative to develop innovative and efficient antibacterial agents, on account of the mounting prevalence of complicated infections induced by multidrug-resistant bacteria. In this work, Chol-b-Lys nanoparticles (CL-NPs) with a diameter of 304.9 nm were prepared by ring-opening polymerization of the monomers cholesterol-ethylenediamine conjugate (Chol-NH2) and L-Lys-N-carboxyanhydrides (L-Lys-NCAs) and further self-assembly. The nanoparticles exhibited biological activity against the drug-resistant and non-resistant bacteria, including Gram-negative E. coli and Gram-positive B. subtilis, E. faecalis and S. aureus, with a MIC value in the range of 32–2 μg mL−1 and a more than 95% bactericidal rate. Importantly, both bacteria treated with CL-NPs didn’t develop resistance even after their 16th passages. Also, the CL-NPs have low toxicity (32 μg mL−1). The mechanism characterization shows that the positively charged nanoparticles are initially gathered on the surface of bacteria by electrostatic adsorption, changes the membrane permeability, inserts into the lipid bilayer bacteria membrane with cholesterol groups, destroys the membrane structure and results in bacterial apoptosis. This work provides a potential approach by mimicking the structure of cell membrane to develop new bactericidal reagent/nanoparticle materials which is hard to cause resistance.
研究领域:
生物材料学
·
微生物学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2022
·
IF 18.027
Zhiwei Huang,Munan Fang,Bin Tu,Jinlei Yang,Zhuang Yan,Haftu Gebrekiros Alemayehu,Zhiyong Tang,Lianshan Li
Low membrane conductivity originated from a high membrane thickness has long been the “Achilles heel” of the conventional polymeric membrane, greatly hampering the improvement of the output power density in osmotic power generation. Herein, we demonstrate a molecularly-thin two-dimensional (2D) covalent organic framework (COF) monolayer membrane, featured with ultimate thickness, high pore density, and tight pore size distribution, which performs as a highly efficient osmotic power generator. Despite the large pore size up to 3.8 nm and relatively low surface charge density of 2.2 mC m–2, the monolayer COF membrane exhibits a high osmotic current density of 16.7 kA m–2 and an output power density of 102 W m–2 under 50 times the NaCl salinity gradient (0.5 M/0.01 M). This superior power density could be further improved to 170 W m–2 in the real seawater/river water gradient system. When the large pore size and low surface charge density are considered, this superior performance is not expected. Computational studies further reveal that the ultimate membrane permeability originated from the high membrane porosity, rather than ion selectivity, plays a dominant role in the production of high current density, especially under high salinity. This work provides an alternative strategy to realize improved output power density in ultrapermeable membranes.
研究领域:
材料科学
·
能源工程
·
环境科学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Chao Jiang,Caijun Luo,Xiaolin Liu,Lei Shao,Youqing Dong,Yingwei Zhang,Feng Shi
The layer-by-layer (LbL) assembled multilayer has been widely used as good barrier film or capsule due to the advantages of its flexible tailoring of film permeability and compactness. Although many specific systems have been proposed for film design, developing a versatile strategy to control film compactness remains a challenge. We introduced the simple mechanical energy of a high gravity field to the LbL assembly process to tailor the multilayer permeability through adjusting film compactness. By taking poly(diallyldimethylammonium chloride) (PDDA) and poly{1–4[4-(3-carboxy-4-hydroxyphenylazo)benzenesulfonamido]-1,2-ethanediyl sodium salt} (PAzo) as a model system, we investigated the LbL assembly process under a high gravity field. The results showed that the high gravity field introduced effectively accelerated the multilayer deposition process by 20-fold compared with conventional dipping assembly; the adsorption rate was positively dependent on the rotating speed of the high gravity equipment and the concentration of the building block solutions. More interestingly, the film compactness of the PDDA/PAzo multilayer prepared under the high gravity field increased remarkably with the growing rotational speed of the high gravity equipment, as demonstrated through comparisons of surface morphology, cyclic voltammetry curves, and photoisomerization kinetics of PDDA/PAzo multilayers fabricated through the conventional dipping method and through LbL assembly under a high gravity field, respectively. In this way, we have introduced a simple and versatile external form of mechanical energy into the LbL assembling process to improve film compactness, which should be useful for further applications in controlled ion permeability, anticorrosion, and drug loading.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
·
2017
·
IF 4.159
Jinyu Li,Guoxin Tan,Bingchao Cheng,Dandan Liu,Weisan Pan
To facilitate the hydrophobic drugs modeled by coumarin-6 (Cou-6) acrossing the cornea to the anterior chamber of the rabbit eye, chitosan (CS) derivatives including chitosan-N-acetyl- l -cysteine (CS-NAC), chitosan oligosaccharides (COS) and carboxymethyl chitosan (CMCS) modified nanostructured lipid carriers (NLCs) were designed and characterized. We found that, with similar size distribution and positive charges, different CS derivatives based on NLCs led to distinctive delivery performance. In vivo precorneal retention study on rabbits revealed that these CS derivatives coating exhibited a stronger resistant effect than Cou-6 eye drops and Cou-6-NLC (P < 0.05), moreover, the AUC (0−∞) , C max and MRT (0−∞) of them followed the sequence of CMCS-Cou-6-NLC < COS-Cou-6-NLC < CS-NAC-Cou-6-NLC. Confocal laser fluorescence microscopy (CLSM) for in vitro corneal penetration study showed that COS-, and CS-NAC-coated NLCs penetrated through the whole corneal epithelium barrier (about 40 μm), while CMCS failed to significantly enhance the intraocular drug penetration as expected, displayed a negligible fluorescence at 30 μm deep. In addition, penetration through the intact cornea was achieved and the penetration levels through the ocular tissues were increased thoroughly for the COS and CS-NAC coating ones compared with CMCS-NLC (P < 0.05), and successfully reduced the conjunctival-to-corneal permeability ratio (ratio (C - S/C) ) thus resulted in a higher bioavailability, which was confirmed by ex vivo fluorescence imaging on ocular tissues. In summary, CS-NAC-NLC and COS-NLC are promising ocular drug delivery systems to achieve prolonged precorneal retention, higher corneal permeability and enhanced ocular bioavailability. And comparatively speaking, CS-NAC-NLC possesses the highest potential for ocular drug delivery.
研究领域:
药剂学
·
眼科研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2025
·
IF 12.1
Fang Zhu,Lanfang Ren,Wenhua Cheng,Haohan Zhou,Yuhan Li,Nan Liu,Guangyu Rong,Yunfeng Liu,Panting Yu,Jia Lv,Yiyun Cheng,Chao Chen
Deferoxamine (DFO) is an FDA-approved naturally occurring iron chelator commonly used to treat transfusion-induced iron overload. The abundant and flexible hydroxamic acid groups in DFO enable exceptional iron binding capacity and high protein binding via hydrogen bonding interactions. However, the applications of DFO to sequester intracellular iron and to deliver proteins inside cells are limited due to poor membrane-permeability. Herein, the fabrication of a dynamic DFO polymer is proposed to achieve robust intracellular protein delivery and efficient mitochondrial iron depletion. Specifically, DFO is grafted onto a polycatechol scaffold via dynamic catechol-boronate chemistry. The obtained DFO polymer shows robust protein binding capacity, and the formed protein complexes show high resistance toward serum proteins. It effectively delivers various cargo proteins into cytosol of treated cells with maintained bioactivity. In addition, the polymer delivers DFO inside cells, and the released DFO efficiently depletes mitochondrial iron, which significantly inhibits mitochondrial oxidative phosphorylation and glycolysis. Remarkable synergistic cytotoxic effects are achieved when the DFO polymer is loaded with toxic proteins. This study provides a general strategy for facile preparation of bioactive polymer toward robust protein delivery, and the designed polymer can be a promising carrier for the delivery of protein therapeutics to treat cancer.
研究领域:
生物化学
·
药物递送
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2019
·
IF 5.108
Lijie Zhou,Biao Ye,Siqing Xia
In this study, we assessed fouling in a membrane bioreactor (MBR) with the addition of suspended aluminum nitride (AlN) nanoparticles (NPs). Three parallel laboratory-scale submerged MBRs were operated with 0, 10, and 50 mg AlN NPs/L for over 70 days. The results showed that the addition of suspended AlN NPs did not significantly affect pollutant biodegradation; there was only a slight decrease in NH 4 + -N removal. Furthermore, the membrane's permeability was increased with effective fouling mitigation by the addition of a high amount of suspended AlN NPs. This was because the suspended AlN NPs decreased the content of polysaccharides in both the extracellular polymeric substances and soluble microbial products, and decreased the sludge floc size. However, the AlN NPs also promoted pore-blocking, particularly standard blocking, which enhanced irreversible fouling. Additionally, owing to the larger ionic radius and higher electronegativity, the AlN NPs inhibited the accumulation of framework components (SiO 2 ). Therefore, suspended AlN NPs resulted in a thinner cake layer.
研究领域:
环境工程
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）