Polymers
·
2022
·Vol. 14
·期 19
·3941
·
IF 4.967
Xu Gao,Jun-Feng Su,Sai Wang,Peng Yang
Artificial skin composites have attracted great interest in functional composite materials. The aim of this study was to prepare and characterize a smart artificial skin composite comprising a bionic microvascular with both self-nourishing and self-healing functions. A poly(vinyl alcohol)–glycerol–gelatin double network organic hydrogel was used as the artificial skin matrix. The hydrogel had high mechanical strength because of the strong hydrogen bond formed between the PVA and glycerol (GL). The gelatin (GEL) increased the toughness and elasticity of the hydrogel to ensure the strength of the artificial skin and fit of the interface with the body. The bionic polyvinylidene fluoride (PVDF) microvascular had excellent thermal stability and mechanical property in artificial skin. Results indicated that self-nourishing was successfully realized by liquid release through the pore structures of the bionic microvascular. The bionic microvascular healed microcracks in the artificial skin when damage occurred, based on a self-healing test.Keywords:artificial skin;self-nourishing;self-healing;microvascular
研究领域:
材料科学
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生物医学工程
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DOI
PubMed
高影响因子
Water
·
2024
·Vol. 16
·期 11
·1475
·
IF 3
Mingxi Li,Yuhang Yang,Weiquan Li,Zhiyi Deng,Jinhua Wu
Zero-valent iron (Fe0) has been extensively used in hexavalent chromium (Cr(VI)) removal from groundwater, but its treatment suffers from interference of humic acid (HA) and ferrochrome precipitate. In this study, a biotic Fe0system was established to address these problems in Cr(VI) removal from HA-rich groundwater by introducing a combination of heterotrophic and hydrogen-autotrophic microorganisms. Due to the formation of HA-Fe complexes and ferrochrome precipitates on the Fe0surface, the HA-abiotic Fe0system obtained a slight Cr(VI) removal of 10.5%. While in the HA-biotic Fe0system, heterotrophic microbes could effectively eliminate HA through biodegradation and decrease HA-Fe complex generation; autotrophic microbes used H2from iron corrosion as electron donors for their metabolism and promoted iron corrosion and active secondary mineral generation (e.g., magnetite and green rust) for Cr(VI) adsorption and reduction. Therefore, a much higher Cr(VI) removal of 84.9% was achieved. Additionally, increasing HA content and extra electron acceptors (e.g., sulfate and nitrate) both boosted Cr(VI) removal, further proving the role of heterotrophic microbes in biodegrading HA for enhanced Cr(VI) elimination. This work presented a feasible strategy to achieve efficient Cr(VI) removal with Fe0by diminishing HA interference and ferrochrome precipitate passivation through the synergistic effect of heterotrophic and hydrogen-autotrophic microorganisms.
研究领域:
环境科学
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微生物学
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DOI
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2024
·
IF 8
Yuhang Yang,Bing Li,Mingxi Li,Zhiyi Deng,Zuoyi Chen,Jinhua Wu
Zero-valent iron (Fe 0 ) usually suffers from organic acid complexation and ferrochrome layer passivation in Cr(VI) removal from bioleached wastewater of Cr slag. In this work, a synergetic system combined Fe 0 and mixed hetero/autotrophic bacteria was established to reduce and stabilize Cr(VI) from bioleached wastewater. Due to bacterial consumption of organic acid and hydrogen, severe iron corrosion and structured-Fe(II) mineral generation (e.g., magnetite and green rust) occurred on biotic Fe 0 surface in terms of solid-phase characterization, which was crucial for Cr(VI) adsorption and reduction. Therefore, compared with the abiotic Fe 0 system, this integrated system exhibited a 6.1-fold increase in Cr(VI) removal, with heterotrophic reduction contributing 3.4-fold and abiotic part promoted by hydrogen-autotrophic bacteria enhancing 2.7-fold. After reaction, the Cr valence distribution and X-ray photoelectron spectroscopy indicated that most Cr(VI) was converted into immobilized products such as Fe x Cr 1-x (OH) 3 , Cr 2 O 3 , and FeCr 2 O 4 by biotic Fe 0 . Reoxidation experiment revealed that these products exhibited superior stability to the immobilized products generated by Fe 0 or bacteria. Additionally, organic acid concentration and Fe 0 dosage showed significantly positive correlation with Cr(VI) removal within the range of biological adaptation, which emphasized that heterotrophic and autotrophic bacteria acted essential roles in Cr(VI) removal. This work highlighted the enhanced effect of heterotrophic and autotrophic activities on Cr(VI) reduction and stabilization by Fe 0 and offered a promising approach for bioleached wastewater treatment .
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Xiaohui Wang,Jia Xin,Mengjiao Yuan,Fang Zhao,Litao Wang
The coupling of microscale zero-valent iron with autotrophic hydrogen bacteria (mZVI-AHB) are often believed to show greater potential than the single abiotic or biotic systems in remediating chlorinated aliphatic hydrocarbon-contaminated groundwater. However, our understanding of the remediation performance of this system under real field conditions, especially by incorporating the concept of sustainable remediation, remains limited. In this study, the performances of the mZVI, H 2 -AHB, and mZVI-AHB systems in dechlorinating groundwater containing complex electron acceptors were compared by evaluating their removal efficiency (RE), reaction products, and electron efficiency (EE), using trichloroethylene (TCE) as the target contaminant and NO 3 − and SO 4 2− as the coexisting natural electron acceptors. Ultimately, which of these systems had TCE removal superiority was dependent on the coexisting electron acceptor. mZVI-AHB and mZVI resulted in more complete dechlorination , whereas H 2 -AHB exhibited higher N 2 selectivity in reducing NO 3 − . Regardless of the coexisting electron acceptor, the mZVI-alone system showed the highest EE. Finally, the sustainability concerns and applicability of the three systems were evaluated on the basis of their TCE RE, complete dechlorination ratio, N 2 selectivity, EE, and cost, which were integrated into a comparison of overall benefits. Our findings provide comprehensive and insightful information on the factors that determine remediation scheme selection in real practice.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2016
·Vol. 18
·期 12
·3537-3548
·
IF 8.506
Jun Ni,Fei Tao,Yu Wang,Feng Yao,Ping Xu
Many plant natural products have remarkable pharmacological activities. They are mainly produced directly by extraction from higher plants, which can hardly keep up with the surging global demand. Furthermore, the over-felling of many medicinal plants has undesirable effects on the ecological balance. In this study, we constructed a photoautotrophic platform with the unicellular cyanobacterium Synechococcus elongatus PCC7942 to directly convert the greenhouse gas CO2 into an array of valuable healthcare products, including resveratrol, naringenin, bisdemethoxycurcumin, p-coumaric acid, caffeic acid, and ferulic acid. These six compounds can be further branched to many other precious and useful natural products. Various strategies including introducing a feedback-inhibition-resistant enzyme, creating functional fusion proteins, and increasing malonyl-CoA supply have been systematically investigated to increase the production. The highest titers of these natural products reached 4.1–128.2 mg L−1 from the photoautotrophic system, which are highly comparable with those obtained by many other heterotrophic microorganisms using carbohydrates. Several advantages such as independence from carbohydrate feedstocks, functionally assembling P450s, and availability of plentiful NADPH and ATP support that this photosynthetic platform is uniquely suited for producing plant natural products. This platform also provides a green route for direct conversion of CO2 to many aromatic building blocks, a promising alternative to petrochemical-based production of bulk aromatic compounds.
研究领域:
生物化学
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合成生物学
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环境科学
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DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Kai Xin,Jun Cheng,Ruhan Guo,Lei Qian,Yulun Wu,Weijuan Yang
To effectively improve biomass growth and flue-gas CO 2 fixation of microalgae, acid-tolerant Euglena gracilis was modified with cobalt-60 γ-ray irradiation and polyethylene glycol (PEG) adaptive screening to obtain the mutant strain M800. The biomass dry weight and maximum CO 2 fixation rate of M800 were both 1.47 times higher than that of wild strain, which was attributed to a substantial increase in key carbon fixation enzyme RuBisCO activity and photosynthetic pigment content. The high charge separation quantum efficiency in PSII reaction center, efficient light utilization and energy regulation that favors light conversion, were the underlying drivers of efficient photosynthetic carbon fixation in M800. M800 had stronger antioxidant capacity in sufficient high-carbon environment, alleviating lipid peroxidation damage. After adding 1 mM PEG, biomass dry weight of M800 reached 2.31 g/L, which was 79.1 % higher than that of wild strain. Cell proliferation of M800 was promoted, the apoptosis and necrosis rates decreased.
研究领域:
植物学
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环境科学
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生物技术
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Qiong Wang,Yuqi Li,Na Chen,Xiaojing Zhang,Yongpeng Ma,Yali Song
Ibuprofen (IBU), a commonly used non-steroidal anti-inflammatory drug, is frequently detected in wastewater treatment systems, where it can interfere with nitrogen removal. This study investigated the effects of IBU on nitrogen removal performance and its biotransformation in a coupled sulfur autotrophic denitrification and anammox (SAD/A) system. Moreover, key parameters, such as nitrogen removal efficiency, microbial activity, community structure, and IBU degradation products, were carefully monitored. While IBU concentrations of up to 1 mg/L had negligible impacts on nitrogen removal efficiency due to the counteracting effects of slight inhibition on anammox and enhancement of sulfur autotrophic denitrification, a significant inhibition of ammonia removal occurred when the concentration increased to 10 mg/L. Quantum chemical analyses revealed that IBU underwent biotransformation through decarboxylation and hydroxylation pathways, leading to the formation of two biotransformation products with high ecological toxicity. This study is the first to elucidate the mechanisms by which IBU influences microbial communities and metabolic activities in SAD/A systems. In addition, it highlights the resilience of these systems in maintaining nitrogen removal efficiency under varying IBU concentrations, as well as the environmental risks posed by the biotransformation products of IBU.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2021
·
IF 10.588
Jianxin Pan,Liangliang Liu,Hanping Pan,Lihui Yang,Meirong Su,Chaohai Wei
Metal sulfide-based biological process is considered as a promising biotechnology for next-generation wastewater treatment . However, it is not clear if simultaneous bio-reduction of nitrate and chromate was achievable in this process. This study aimed to evaluate the feasibility of metal sulfides (FeS and MnS) on simultaneous denitrification and chromate reduction in autotrophic denitrifying column bioreactors . Results showed that simultaneous reduction of nitrate and chromate was achieved using metal sulfides (FeS and MnS) as electron donors, in which sulfate was the sole soluble end-product. Apart from the sulfur element in the metal sulfides, Fe(II) and Mn(II) were also involved in nitrate and chromate reduction as indicative by the formation of their oxidative states compounds. In microbial communities , SHD-231 and Thiobacillus were the most predominant bacteria, which might have played important roles in simultaneous denitrification and chromate reduction. Compared to FeS, MnS showed a higher performance on nitrate and chromate removal, which could also reduce the toxic inhibition of chromate on nitrate reduction . According to results of XRD and XPS, as well as a lower sulfate production in the FeS system, FeS might have been covered easily to hydroxides due to its bio-oxidation, which limited mass transfer efficiency and bio-availability of FeS. The findings in this study offered insights in the development of promising approaches for the treatment of toxic and hazardous compounds using metal sulfide.
研究领域:
环境科学
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微生物学
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DOI
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 11
·2412
·
IF 4.6
Minyi Zhu,Minhui Ma,Shuo Chen,Rongfang Yuan,Shaona Wang
Pollution caused by N and P is a significant contributor to water eutrophication. While traditional biological treatment processes can remove some N and P elements from water, the effluent quality often fails to meet the stringent requirements of sensitive areas. The autotrophic denitrification’s simultaneous nitrogen and phosphorus removal pro-cess, known for its low operating cost and minimal sludge production, has garnered considerable attention from researchers. In this study, natural pyrite was used for the removal of nitrogen and phosphorus in a denitrification system, and the underlying mechanisms were elucidated. The results indicate that the N and P removal efficiency was influenced by empty bed contact time (EBCT) and the pH value. The highest NO 3 − -N removal rate of 90.24% was achieved at an EBCT of 8 h, while the PO 4 3− -P removal rate reached 81.58% at an EBCT of 12 h. The addition of a carbon source enhanced the synergistic autotrophic/heterotrophic denitrification, significantly improving phosphorus removal with an increasing C/N ratio. Microbial characteristics analysis revealed that, at the phylum level, Chlorobiota , Bacteroidota , and Chloroflexota played a crucial role in heterotrophic autotrophic denitrification. At the genus level, Thauera , Aridibacter , and Gemmatimonas were key players in heterotrophic denitrification, while Thiobacillus , Rhodoplanes , and Geobacter were associated with autotrophic denitrification.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
Journal of Water Process Engineering
·
2025
·
IF 6.7
Ze-Tong Feng,Yan Yu,Ying-Jun Sun,Jia-Min Zhou,Xin Ma,Fei Ding,Yi-Liu Zheng,Ren-Cun Jin,Chun-Qiang Shen,Qian-Qian Zhang
This study systematically evaluated the effects of intermittent versus continuous addition of zero-valent iron (ZVI) as exogenous enhancers on nitrogen removal performance in denitrification systems. Results shown that under carbon-to‑nitrogen ratios (C/N) was 3, 2, and 1, continuous addition of ZVI significantly improved total nitrogen removal efficiency (TNRE) by 8.57 %, 19.34 %, and 11.45 %, respectively, compared to the control system. Concurrently, extracellular polymeric substance (EPS) production increased by 32.43 %, 62.91 %, and 35.93 %, to that of intermittent ZVI addition system at the same phases. The continuous ZVI-added system enriched more mixotrophic denitrification bacteria and increased soluble iron ion concentrations through EPS-mediated electron shuttling and endogenous protection mechanisms. While, under C/N was 0, the TNRE in continuously ZVI-added system reduced to 8.3 ± 3.24 % due to iron mineral crust formation. Specifically, the TNRE of system with intermittent addition of ZVI stabilized at 17.03 ± 3.24 %, enriched more autotrophic denitrification bacteria and avoided excessive accumulation of iron oxides. Therefore, this experiment provided new insights into the stable operation of the ZVI-enhanced denitrification process for treating low C/N wastewater.
研究领域:
环境科学
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微生物学
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Zhen Bi,Wenjing Zhang,Ge Song,Yong Huang
Anammox bacteria are chemolithotrophic organisms growing on the conversion of ammonium and nitrite with bicarbonate as the sole carbon source. Meanwhile, anammox bacteria display a metabolic versatility to sustain their metabolism. However, there is less attention on the Fe 0 /Fe 2+ -dependent autotrophic denitrification by anammox consortia. In this study, the autotrophic nitrate reduction using different valence of iron (Fe 0 , Fe 2+ and Fe 0 + Fe 2+ , respectively) as electron donors by anammox consortia were firstly explored in continuous feeding mode. Results revealed anammox consortia showed high adaptability to the niche wherein containing nitrate and iron. They could generate nitrite and ammonium from iron-dependent nitrate reduction, and hence support their central metabolism. During 60-days operation, the maximum nitrate and total nitrogen removal efficiency reached 88.43% and 80.77%, respectively, with coexistence of Fe 0 and Fe 2+ . The expression of key functional genes involved in nitrate reduction (including narG, napA and nrfA ) in 16S rRNA level revealed the coupling of dissimilatory nitrate reduction to nitrite, dissimilatory nitrite reduction to ammonia (DNRA), and anammox processes possibly play pivotal role in nitrogen loss under Fe 0 /Fe 2+ condition. Meanwhile, abiotic reduction by Fe 0 /Fe 2+ also contributed nitrate reduction to provide nitrite and ammonium for anammox consortia. Activities of two vital enzymes hydrazine dehydrogenase (HDH) and nitrate oxidoreduetase (NAR) also inferred higher microbial activities with co-existence of Fe 0 and Fe 2+ . The present study confirms and further extends the versatile metabolisms of Anammox consortia, also it can help to circumvent the accumulation of nitrate produced by anammox process itself and increase the quality of discharge.
研究领域:
微生物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Jinbin Han,Xiang Qi,Peng Liang
Excess nitrate presented in natural water body and drinking water has been a challenge for maintaining safe ecosystem and human health. Sulfur autotrophic denitrification is proved a feasible technology to remove nitrate from water environment. However, comparatively low rate of sulfur autotrophic denitrification needs to be addressed before wide application of this technology, which is a result of the low solubility of elemental sulfur. Therefore, this study employed bovine serum albumin (BSA) as a supplementary material to modify the elemental sulfur for improved sulfur autotrophic denitrification rate. Artificial biofilm of Thiobacillus denitrificans was prepared and employed in experiments. By testing different amount of BSA applied in both elemental sulfur and the biofilm, including 1 %, 2 % and 4 % mass ratios, it was found that larger employment of BSA had significant effect in increasing the denitrification rate. Particularly when 4 % BSA was added into elemental sulfur, the highest denitrification rate reached 26.8 mg-N/(L·d), 3.7 times of the control group. Meanwhile, the largest reaction rate constant was achieved, 4.13 mg 0.5 /(L 0.5 ·d), 2.78 times of the control group. This effect was attributed to promoted conversion of elemental sulfur to polysulfide that was easily utilized by sulfur-oxidizing bacteria. A long-term operation (14 days) of packed bed reactor filled with sulfur particles and 1 % BSA delivered a much faster start-up than the control and outperformed it with better denitrification performance all-through the experiment. This result evidenced again that BSA could make a highly effective supplement in sulfur autotrophic denitrification.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）