Biomaterials Science
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2018
·Vol. 6
·期 11
·3030-3041
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IF 5.831
Wanjun Hu,Zhigang Zhang,Shenglin Lu,Tianzhu Zhang,Naizhen Zhou,Pengfei Ren,Faming Wang,Yang Yang,Zhenling Ji
Abdominal adhesion to polypropylene (PP) mesh remains one of the major complications in hernia repair. Thus, a challenge exists to endow PP mesh with powerful anti-adhesion properties in hernia repair. To investigate potential options, the assembled PP mesh was developed with effective anti-adhesion properties through an in situ coating of the mesh surface with self-fixable and biodegradable mussel-inspired hydrogels. Through mixing oxidized-carboxymethylcellulose functionalized with dopamine (OCMC-DA) with carboxymethylchitosan (CMCS), a layer of hydrogel (OCMC-DA/CMCS) can be formed in situ on the PP mesh without the addition of crosslinking agents; the dopamine then acts as an immobilization group to fix these hydrogels to the PP mesh and the tissue surface. In this way, the assembled PP mesh (OCMC-DA/CMCS/PP) was obtained. The properties of the OCMC-DA/CMCS hydrogels were optimized, and the OCMC-DA4/CMCS hydrogel was selected to construct the assembled PP mesh. The lap-shear test revealed that OCMC-DA4/CMCS has tissue-adhesive properties. In vitro cell tests proved the excellent biocompatibility of the hydrogel. An optimized bioabsorption time and significant anti-adhesion properties were demonstrated through an in vivo test with a rat model. The adhesion area and tenacity of the OCMC-DA4/CMCS/PP group were more than 80% lower than those of the native PP mesh group and created a slightly inflammatory reaction.
研究领域:
生物医学工程
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材料科学
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外科手术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Hang Yuan,Guanzhang Liu,Yaxin Chen,Zhiwei Yi,Wenhui Jin,Guangya Zhang
Enzymatic degradation of polyethylene terephthalate (PET) suffered from challenges such as complex and costly enzyme preparation , difficult access to PET substrates, poor reusability of free enzymes and sometimes MHET inhibitions. Herein, we propose an “all-in-one” strategy to address these issues with a well-designed elastin-like polypeptides (ELPs) tag. The preparation of the ELPs-tagged cutinase (ET-C) was efficient and easy to scale up by centrifugation , with an activity recovery of 57.55 % and a yield of 160 mg/L. Besides, the activity of the ET-C was 1.3 and 1.66-fold higher in degrading PET micro- and macro-plastics compared to wild-type cutinase. The self-immobilized cutinase (ET-C@SiO 2 ) obtained by the ELPs-mediated biosilicification exhibited high loading capacity, activity, and thermostability and maintained 77.65 % of the original activity after 10 reuses. Interestingly, the product of the ET-C was TPA, whereas the wild-type was TPA and MHET. This is a simple way to release the intermediates inhibition compared with the existing methods. Our results demonstrated the feasibility of the versatile ELPs tag, which will pave an alternative economic way for scalable PET biodegradation.
研究领域:
生物化学
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2015
·
IF 4.321
Qilei Zhang,Tao Lu,Dong-Mei Bai,Dong-Qiang Lin,Shan-Jing Yao
Biosorbents for wastewater treatments is one of the most widely studied techniques in environmental and bioresource research. A marine-derived Penicillium janthinellum strain fungus was used in this study to prepare self-immobilized mycelial pellets. Fe 3 O 4 nanoparticles were added in the culturing media, which were automatically incorporated in the mycelial pellets during culturing process to endow the pellets with magnetic properties. These magnetic mycelial pellets were used as biosorbents for water treatments , and their morphology was characterized by microscopic observation, Energy dispersive X-ray analysis, Fourier transform infrared spectroscopy and thermal gravimetric analysis . The effects of nanoparticles in culture media on pellet formation were discussed and the content of nanoparticle in culturing media was optimized. Study on Congo red adsorption from aqueous solutions showed that the pellets with 71.6 wt% Fe 3 O 4 nanoparticles had a maximum adsorption capacity of 102.4 mg/g pellets (360.6 mg/g mycelia), and the solution heated by magnetic induction showed faster adsorption rates than that of bulk liquid heating processes under the same adsorption temperature. These pellets were easily separated by magnetic forces and the magnetic induction heating is beneficial in energy saving when comparing to traditional heating processes.
研究领域:
环境科学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2025
·Vol. 17
·期 8
·4783-4795
·
IF 5.1
Hao Yang,Yi Zhuang,Yulin Jiang,Huilun Xu,Zheng Liu,Yubao Li,Shuyu Zhang,Tao Guo,Lin Qi,Li Zhang
Continuous microenvironment modulation is an ongoing challenge in wound dressing, which includes excessive exudate absorption, oxygen delivery, bacterial inhibition and angiogenesis. Herein, we developed an in situ construction strategy to fabricate a self-retaining double-layered wound dressing, where the top layer precursor was composed of Ca2+-containing polyvinyl butyral (PVB) solution dispersed with hydroxypropyl methylcellulose (HPMC) particles, and the bottom one consisted of sodium alginate (Alg) solution blended with Ag-doped mesoporous bioactive glass powders (Ag-MBG). When in use, both precursors were simultaneously squeezed out from the twin nozzles connected to the individual chambers of a twin-chambered syringe, whereby Ca2+ in the top layer rapidly migrated downwards to crosslink Alg in the bottom layer, leading to the formation of an Alg/Ag-MBG (AA) functional hydrogel for filling an irregular wound. Meanwhile, with the rapid evaporation of low-boiling solvents, the top layer changed into a PVB/HPMC (PH) membrane covering the AA hydrogel and adhering to the surrounding healthy skin to fix the dressing. Practically, HPMC particles in the top layer acting as “micropumps” could drain the wound exudate out, while Ag-MBG in the bottom layer endowed the dressing with anti-bacterial, hemostatic, and pro-healing functions. The integrally constructed PH-AA dressing achieved over 99% bacterial elimination against both E. coli and S. aureus. Biological assessments indicated that the double-layered dressing possessed excellent biocompatibility and enhanced wound healing, demonstrating a wound closure rate of >97% at day 15. This study provides a facile method to directly construct multi-layer dressings on wounds to meet various wound care requirements.
研究领域:
生物材料学
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组织工程
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医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）