Forensic Science Medicine and Pathology
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2015
·Vol. 11
·期 4
·570-576
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IF 1.983
Zhang Lin,Ma Jinghong,Li Shangxun,Xue Rui,Jin Ming,Zhou Yiwu
Diphenidol hydrochloride (DPN), a nonphenothiazinic antiemetic agent used primarily in patients with Meniere disease and labyrinthopathies to treat vomiting and vertigo, is considered to be a relatively safe drug. Since it was first approved in the United States in 1967, this drug has been widely used in Latin America and Asia and has contributed to sporadic suicidal and accidental poisonings in mainland China and Taiwan. However, its toxic or lethal concentration ranges have not yet been determined. We report a case of a 23-year-old female who suffered from DPN poisoning that resulted in death. At autopsy, there were no typical pathological findings, except for cerebral edema with high acetylcholinesterase expression. Postmortem analysis of DPN revealed 45 µg/ml in heart blood, 39 µg/ml in femoral vein blood, 141 µg/g in the liver, and 53 mg in the gastric contents. These concentrations indicated that the cause of death was DPN poisoning. The circumstances indicated that the manner of death was suicide. We also present a retrospective study, in which we review and summarize the literature from 1998 to 2014 and describe 16 cases of poisoning, including information from autopsy reports and postmortem drug concentrations. In forensic practice, drug residues at the scene, patients with convulsions and disturbance of consciousness, and rapidly occurring deaths, should draw attention to the possibility of this drug. Toxicological analysis and the exclusion of other diseases may ultimately be used to confirm DPN poisoning.
研究领域:
法医学
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毒理学
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临床医学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 8
·5577-5587
·
IF 3.9
Kai Li,Sinan Tian,Ke Sun,Qingguo Su,Yanhui Mei,Wenjie Niu
Prostate cancer is the most common malignant tumor in the male reproductive system, and its incidence increases with age. Chemotherapy is one of the main strategies for treating prostate cancer, but it often comes with unavoidable side effects. Nanocarriers can improve drug utilization and targeting, and cationic carriers can also carry nucleic acids for gene therapy. In this study, we prepared a cationic micelle constructed from a polyprodrug that can deliver both chemotherapeutic drugs and nucleic acids simultaneously. The typical chemotherapeutic drug hydroxycamptothecin (HCPT) was linked by reactive oxygen species (ROS)-responsive coupling agents and forms amphiphilic block polymers with low molecular weight polyethyleneimine (PEI). The resulting cationic micelles can be triggered by high levels of ROS in tumor cells and collapse to release HCPT and suicide genes to kill tumor cells. At the same time, it reduces the killing of normal cells. In prostate cancer cells, it has been confirmed that the co-delivery carriers combined with chemotherapy and a suicide gene prodrug system have shown an ideal therapeutic effect on prostate cancer.
研究领域:
肿瘤学
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药剂学
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分子生物学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Yanhui Lu,Yanze Meng,Hui Li,Yunyang Bai,Ying He,Boon Chin Heng,Yating Song,Xiaoyu Han,Yifei Zhang,Youde Liang,Lei Zhang,Yaojin Wang,Yaru Guo,Mingming Xu,Xuliang Deng,Xuehui Zhang
The design of antibacterial functions in dental resin composites is a key approach to preventing secondary caries. Although conventional composite resins incorporated with antimicrobial agents can indeed exhibit bactericidal effects, these lack long-lasting antimicrobial activity and may exert cytotoxic effects, thus, causing biosafety concerns. Here, we developed a universal, nondestructive, and self-bactericidal strategy for fabricating dental resin nanocomposites without additional devices or power sources. This was achieved by incorporating a ceramic nanofiller with pyrocatalytic activity, which is activated by ubiquitous oral activity-induced temperature fluctuations. By optimizing the content of BaSrTiO3 (BST) pyroelectric fillers, the BST-resin nanocomposites exhibited a positive pyroelectric effect, as well as reactive oxygen generation capacity under physiological temperature fluctuations associated with food/drink intake and speech. The initial adhesion and growth of S. mutans were significantly inhibited by pyroelectric BST-resin nanocomposites. Subsequent biofilm formation was suppressed by pyroelectric effects activated by temperature fluctuations. Moreover, the pyrocatalysis-based resin nanocomposites displayed excellent therapeutic biocompatibility and excellent mechanical properties, which are comparable to those of commercial resins. Hence, our findings provide an innovative strategy for addressing the antibacterial technical requirements of dental resin nanocomposites.
研究领域:
牙科学
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材料科学
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生物医学工程
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DOI
PubMed
高影响因子
Bio-protocol
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2024
·
IF 1
Tingfeng Cheng,Tongling Ge,Xinyue Zhao,Zhu Liu,Lei Zhao
Erwinia persicina is a gram-negative bacterium that causes diseases in plants. Recently, E. persicina BST187 was shown to exhibit broad-spectrum antibacterial activity due to its inhibitory effects on bacterial acetyl-CoA carboxylase, demonstrating promising potential as a biological control agent. However, the lack of suitable genetic manipulation techniques limits its exploitation and industrial application. Here, we developed an efficient transformation system for E. persicina. Using pET28a as the starting vector, the expression cassette of the red fluorescent protein-encoding gene with the strong promoter J23119 was constructed and transformed into BST187 competent cells to verify the overexpression system. Moreover, suicide plasmid-mediated genome editing systems was developed, and lacZ was knocked out of BST187 genome by parental conjugation transfer using the recombinant suicide vector pKNOCK-sacB-km-lacZ. Therefore, both the transformation and suicide plasmid-mediated genome editing system will greatly facilitate genetic manipulations in E. persicina and promote its development and application. Key features • Our studies establish a genetic manipulation system for Erwinia persicina , providing a versatile tool for studying the gene function of non-model microorganisms. • Requires approximately 6-10 days to complete modification of a chromosome locus.
研究领域:
微生物学
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分子生物学
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基因工程
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Kaigeng Hu,Yu Liu,Qingqing Wang,Yan Xiong,Zirun Guo,Zhenzhen Weng,Yu Liu,Yini Zhang,Hongguang Wu,Fanrong Ai,Xiaolei Wang
Short-term memory refers to processing information during short-term storage and manipulation which is affected by a variety of factors. If there exists a biocompatible material that can effectively enter the brain, remove some short-term memories (such as car accident, murder, etc.), and be excreted from the body automatically, it will be expected to significantly reduce level of depression, anxiety and even the odds of suicide. Herein, we synthesize the bovine serum albumin (BSA)-templated copper nanoclusters (BSA-CuNCs) and BSA-templated silver nanoclusters (BSA-AgNCs) respectively then discuss their potential as short-term memory “erasers”. In vivo experiments show that both CuNCs and AgNCs can effectively penetrate the blood–brain barrier (BBB) and decrease the recognition memory of mice by inhibiting neuronal cell conduction within 24 h. Interestingly, after 3 weeks, the discrimination index (DI) of the BSA-CuNCs-treated mice increases again and the memory can be recovered. In contrast, mice of the BSA-AgNCs group do not regain their memory. In addition, BSA-CuNCs exhibit good biocompatibility and can be metabolically cleared by the organism, which makes BSA-CuNCs intriguing potential for short-term memory erasure.
研究领域:
神经科学
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纳米技术
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药理学
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生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF CONTROLLED RELEASE
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2015
·
IF 7.705
Tian-Yuan Zhang,Bing Huang,Hai-Bin Wu,Jia-He Wu,Li-Ming Li,Yan-Xin Li,Yu-Lan Hu,Min Han,You-Qing Shen,Yasuhiko Tabata,Jian-Qing Gao
The success of conventional suicide gene therapy for cancer treatment is still limited because of lack of efficient delivery methods, as well as poor penetration into tumor tissues. Mesenchymal stem cells (MSCs) have recently emerged as potential vehicles in improving delivery issues. However, these stem cells are usually genetically modified using viral gene vectors for suicide gene overexpression to induce sufficient therapeutic efficacy. This approach may result in safety risks for clinical translation. Therefore, we designed a novel strategy that uses non-viral gene vector in modifying MSCs with suicide genes to reduce risks. In addition, these cells were co-administrated with prodrug-encapsulated liposomes for synergistic anti-tumor effects. Results demonstrate that this strategy is effective for gene and prodrug delivery, which co-target tumor tissues, to achieve a significant decrease in tumor colonization and a subsequent increase in survival in a murine melanoma lung metastasis model. Moreover, for the first time, we demonstrated the permeability of MSCs within tumor nests by using an in vitro 3D tumor spheroid model. Thus, the present study provides a new strategy to improve the delivery problem in conventional suicide gene therapy and enhance the therapeutic efficacy. Furthermore, this study also presents new findings to improve our understanding of MSCs in tumor-targeted gene delivery.
研究领域:
癌症生物学
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基因治疗
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干细胞研究
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药物科学
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肿瘤生物学
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DOI
PubMed
高影响因子
BIOMATERIALS
·
2024
·
IF 12.8
Jian He,Xiuhui Lin,Dongxiao Zhang,Huiqun Hu,Xiaoyuan Chen,Feng Xu,Min Zhou
The hypometabolic and nutrient-limiting condition of dormant bacteria inside biofilms reduces their susceptibility to antibacterial agents, making the treatment of biofilm-dominating chronic infections difficult. Herein, we demonstrate an intratracheal aerosolized maltohexaose-modified catalase-gallium integrated nanosystem that can ‘wake up’ dormant Pseudomonas aeruginosa biofilm to increase the metabolism and nutritional iron demand by reconciling the oxygen gradient. The activated bacteria then enhance suicidal gallium uptake since gallium acts as a 'Trojan horse' to mimic iron. The internalized gallium ions disrupt biofilms by interfering with the physiological processes of iron ion acquisition and utilization, biofilm formation, and quorum sensing. Furthermore, aerosol microsprayer administration and bacteria-specific maltohexaose modification enable accumulation at biofilm-infected lung and targeted release of gallium into bacteria to improve the therapeutic effect. This work provides a potential strategy for treating infection by reversing the dormant biofilm's resistance condition.
研究领域:
微生物学
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药学
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纳米技术
·
传染病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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