TALANTA
·
2018
·
IF 4.244
Zi-Han Chen,Xin-Yue Han,Ling-Xue Deng,Zi-Yang Lin,Fang-Ya Mu,Shengqiang Zhang,Guoyue Shi,Min Zhang
Tb 3+ -doped carbon dots (Tb 3+ @CDs) were prepared in a facile hydrothermal method by using ammonium citrate as carbon source and Tb 3+ as dopant. A 15-bp GT-rich single-strand DNA (ssDNA) was introduced to sensitize Tb 3+ via the antenna effect for generating two fluorescence signals (CDs and Tb 3+ ), forming a conjugate of Tb 3+ @CDs/ssDNA. The ratiometric fluorescence of Tb 3+ @CDs/ssDNA could be reversibly regulated by Ag + and Cys, in which the fluorescence peak at 546 nm of Tb 3+ could be switched to “On” or “Off” as the signal indicator while the fluorescence peak at 444 nm of CDs remained constant as the build-in reference. The proposed Ag + /Cys-mediated reversible fluorescence changes in Tb 3+ @CDs/ssDNA was also proven for the design of a self-calibrating ratiometric fluorescence logic system. By integrated with the specific reaction between H 2 O 2 and Cys, Tb 3+ @CDs/ssDNA was applied for ratiometric fluorescence detection of H 2 O 2 . More importantly, the sensing strategy could be further successfully extended to the monitoring of H 2 O 2 -produced oxidase-related reactions, such as GOx-biocatalyzed oxidation of glucose (the limit of detection: 0.06 μM) and was well applied in rat serum compared to commercial kits. This work unveiled a novel ratiometric fluorescent design, which is cost-effective, simple to prepare and easy-to-use without chemical modification or fluorescence labeling .
研究领域:
生物化学
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分析化学
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生物传感
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Xiaoyi Lv,Fei Tan,Tian Miao,Jihui Zhang,Zhiwen Zhang,Bo Cui,Yishan Fang
Electrochemiluminophore system with potential-resolved has exhibited giant potentiality for the application of bioassays in recent years, however, rarely studies toward the novel luminophore with potential-resolved electrochemiluminescence (PRECL) have been reported. In this work, a novel organoboron complex microrod (IBPHF) with PRECL property was synthesized, which emitted two strongest electrochemiluminescence (ECL) signals in the anode region at around 1.5 V and cathode region at around −1.5 V by using tri- n -propylamine (TPA)/K 2 S 2 O 8 as coreactant. The relevant ECL properties and luminescence mechanisms have been discussed in detail. Moreover, electroactive substrate of three-dimensional porous starch carbon nanomaterials (3D-PSC NPs) were designed to capture antibodies. As a result, the competitive potential-resolved differential electrochemiluminescence immunosensor was fabricated, and greatly enhanced sensitivity was achieved by using IBPHF by using IBPHF as a carrier to label competing antigens. In the presence of aflatoxin B 1 (AFB 1 ), due to the competitive mechanism and the specific recognition of antigen–antibody, the well-prepared immunosensor performed a quantitative decreasement in the range from 50 fg/mL to 100 ng/mL with a minimum detectable limit of 16.44 fg/mL by using the intensity difference (△ECL) (ECL anode -ECL cathode ). The self-correction of △ECL extremely enhanced the accuracy and reliability of the sensor. Furthermore, the designed sensor achieved excellent detection towards AFB 1 in walnut samples, and performed a bright application for trace target detection in future.
研究领域:
分析化学
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生物医学工程
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Tianmiao Wang,Jing Hu,Jingyuan Xu,Yibing Ji,Ruijun Li
The development of a self-calibrating ratio fluorescence probe without the need for additional substrates is a major advancement in biosensing. In this study, at room temperature, a self-calibrating infinite coordination polymer (SSA-Tb-ATP ICPs) has been proposed by self-assembling adenosine triphosphate (ATP) with 5-sulfosalicylic acid (SSA) and Tb3+. Due to the antenna effect, SSA-Tb-ATP ICPs exhibited strong green fluorescence emission of Tb3+ (at 547 nm) and blue fluorescence emission of SSA (at 407 nm). This material offers several advantages over existing detection methods, including simplicity of synthesis and exceptional sensitivity. Our self-calibrating SSA-Tb-ATP ICPs demonstrated excellent performance in detecting alkaline phosphatase (ALP) and phosphate (Pi) in both serum and environmental samples with detection limits of 0.076 U/L and 0.025 μM, respectively. Moreover, we successfully employed the SSA-Tb-ATP ICPs to perform cellular imaging of ALP in both hepatocellular carcinoma cells (HepG2) and normal liver cells (LO2), representing a significant advancement in ALP detection and imaging. The simplicity of the synthesis and high sensitivity make this probe a promising tool for early diagnosis of hepatocellular carcinoma in clinical settings and environment analysis.
研究领域:
生物化学
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医学诊断
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环境科学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2025
·Vol. 13
·期 38
·32731-32740
·
IF 9.5
Ying Li,Dandan Song,Bing Liu,Xin Li,Jie Huang,Haiyan Zhu,Gang Shi
A surface-enhanced Raman spectroscopy sensor combined with the molecular imprinting technique (MIT-SERS) has the ability of high sensitivity and specific recognition, but its accurate quantitative analysis and high-stability reuse are poor. To solve the above problems, a novel MIT-SERS sensor with internal standard self-correction and spatially separated photocatalysis is designed. This MIT-SERS sensor is composed of a SERS substrate and a MIT recognizer. Photocatalyst TiO2, co-reductant catalyst Ag and co-oxidant catalyst Fe2O3 are the main materials of the SERS substrate. The occupying molecule C3H6S and the template molecule 4-ATP are adsorbed on the surface of Ag and Fe2O3 in turn. The molecularly imprinted layer is SiO2. It is worth noting that the space-confined assembly of C3H6S and 4-ATP ensures that C3H6S is protected and 4-ATP is completely degraded during the photocatalysis process, realizing the reusability of the MIT-SERS sensor. In addition, SiO2 is used as an internal standard to improve the accuracy of quantitative analysis of the sensor. For this MIT-SERS sensor, the detection limit (LOD) is as low as 2.24 × 10−11 M and the linear fit (R2) of quantitative analysis is as high as 0.9975. During repeated use, the relative standard deviation (RSD) of the corrected signals from the MIT-SERS sensor is as low as 1.27%.
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高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 5
·1-8
·
IF 5.833
Sun Ying,Lin Tianran,Zeng Cuihong,Jiang Gaoyan,Zhang Xuanhan,Ye Fanggui,Zhao Shulin
A self-correcting fluorescent assay of tyrosinase (TYR) was developed by utilization of Fe-MIL-88B-NH 2 as a peroxidase-like nanozyme and a capture probe. Fe-MIL-88B-NH 2 nanozyme was selected as an electron donor, and the oxidization product (dopamine- o -quinone) acts as an energy acceptor. First, TYR catalyzes the oxidation of tyramine hydrochloride to dopamine and then to dopamine- o -quinone. Second, Fe-MIL-88B-NH 2 with intrinsic peroxidase-like activity decomposes H 2 O 2 to produce ·OH radicals, which further accelerate the oxidation of dopamine to dopamine- o -quinone. Excessive H 2 O 2 and ·OH radicals reduce the interferences from ascorbic acid at the same time providing a self-correcting ability. Dopamine- o -quinone reacts with -NH 2 groups on the ligand of Fe-MIL-88B-NH 2 through Michael reaction which results in fluorescence quenching. Under 365-nm excitation, the fluorescence emission intensity at 452 nm gradually decreased with increasing TYR concentration varying from 0 to 10 U mL −1 . The linear range is from 1 to 5 U mL −1 and the detection limit is 0.05679 U mL −1 . This self-correcting fluorescent assay of tyrosinase exhibits good sensitivity and selectivity which is also successfully applied for tyrosinase inhibitor detection. Graphical abstract
Schematic representation of fluorescent assay for tyrosinase determination based on Fe-MIL-88B-NH 2 nanozyme. A self-correcting fluorescent assay for tyrosinase was developed based on the Fe-MIL-88B-NH 2 nanozyme.
研究领域:
分析化学
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生物化学
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纳米技术
·
酶学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）