ACS Synthetic Biology
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2021
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IF 5.11
Ming Zhao,Mingrui Wang,Shuiling Wang,Liangbin Xiong,Bei Gao,Min Liu,Xinyi Tao,Feng-Qing Wang,Dongzhi Wei
Streptomyces species possess strong secondary metabolism, the switches of which from the primary metabolism are complex and thus a challenge to holistically optimize their productivities. To avoid the complex switches and to reduce the limitations of different metabolic stages on the synthesis of metabolites, we designed a Streptomyces self-sustained system (StSS) that contains two functional modules, the primary metabolism module (PM) and the secondary metabolism module (SM). The PM includes endogenous housekeeping sigma factor σhrdB and σhrdB-dependent promoters, which are used to express target genes in the primary metabolism phase. SM consists of the expression cassette of σhrdB under the control of a secondary metabolism promoter, which maintains continuous activity of the σhrdB-dependent promoters in the secondary metabolism phase. As a proof-of-principle, the StSS was used to boost the production of some non-toxic metabolites, including indigoidine, undecylprodigiosin (UDP), ergothioneine, and avermectin, in Streptomyces. All these metabolites can undergo a continuous production process spanning the primary and secondary metabolism stages instead of being limited to a specific stage. Scale-up of UDP fermentation in a 4 L fermentor indicated that the StSS is a stable and robust system, the titer of which was enhanced to 1.1 g/L, the highest at present. This study demonstrated that the StSS is a simple but powerful strategy to rationally engineer Streptomyces cell factories for the efficient production of non-toxic metabolites via reconstructing the relationships between primary and secondary metabolism.
研究领域:
微生物学
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合成生物学
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代谢工程
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DOI
PubMed
高影响因子
Nature Communications
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2022
·Vol. 13
·期 1
·1-8
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IF 17.694
Tan Jin,Fang Sunmiao,Zhang Zhuhua,Yin Jun,Li Luxian,Wang Xiang,Guo Wanlin
Generating sustainable electricity from ambient humidity and natural evaporation has attracted tremendous interest recently as it requires no extra mechanical energy input and is deployable across all weather and geography conditions. Here, we present a device prototype for enhanced power generation from ambient humidity. This prototype uses both heterogenous materials assembled from a LiCl-loaded cellulon paper to facilitate moisture adsorption and a carbon-black-loaded cellulon paper to promote water evaporation. Exposing such a centimeter-sized device to ambient humidity can produce voltages of around 0.78 V and a current of around 7.5 μA, both of which can be sustained for more than 10 days. The enhanced electric output and durability are due to the continuous water flow that is directed by evaporation through numerous, negatively charged channels within the cellulon papers. The voltage and current exhibit an excellent scaling behavior upon device integration to sufficiently power commercial devices including even cell phones. The results open a promising prospect of sustainable electricity generation based on a synergy between spontaneous moisture adsorption and water evaporation.
研究领域:
能源技术
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材料科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2024
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IF 8.3
Xiuli Xie,Xiaotong Wang,Yang Zhang,Long Fang,Jiangshan Feng,Shaohong Liu,Dingshan Yu,Fangming Zhu,Xudong Chen
Herein, we develop a new intelligent moisture-sensitive hybrid aerogel by evenly embedding a proton-conductive covalent organic framework (COF-2SO3H) into a carboxylated cellulose nanofiber network (CNF-C) for water harvesting and spontaneous sustained electricity production from ambient humidity and human respiration. Our strategy first exploits the “suspending agent” role of CNF-C to stably disperse COF materials in water for forming uniform hierarchical hybrid structures. By utilizing the synergy of COF-2SO3H and CNF-C together with their inherent structure merits and surface group effects, the hybrid aerogel displays increased water uptake and ion conductivity. Upon asymmetric moisturization, it can create a self-maintained moisture gradient to engender a concentration difference for mobile Na+ and H+, resulting in efficient charge separation and diffusion. Thus, the hybrid aerogel-based coin-type generator achieves a continuous output voltage of ∼0.55 V for at least 5 h in ambient environments in contrast to that using pure CNF-C and carbon-based generators with transient voltage response. Intriguingly, the wearable generator with an aerogel in a mask is more sensitive to human respiration and achieves repeatable and reliable self-charge for persistent electricity along with an increased output voltage of up to 1.0 V and much faster self-charge (only 3 min), both of which surpass most reported moisture-enabled generators.
研究领域:
材料科学
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能源
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环境科学
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纳米技术
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2025
·
IF 26.8
Wenshuai Han,Jiayi Ding,Bo Qiao,Yingjie Yu,Hao Sun,Daniel Crespy,Katharina Landfester,Xiangzhao Mao,Shuai Jiang
Metastasis, the leading cause of mortality in cancer patients, presents challenges for conventional photodynamic therapy (PDT) due to its reliance on localized light and oxygen application to tumors. To overcome these limitations, a self-sustained organelle-mimicking nanoreactor is developed here with programmable DNA switches that enables bio-chem-photocatalytic cascade-driven starvation-photodynamic synergistic therapy against tumor metastasis. Emulating the compartmentalization and positional assembly strategies found in living cells, this nano-organelle reactor allows quantitative co-compartmentalization of multiple functional modules for the designed self-illuminating chemiexcited PDT system. Within the space-confined nanoreactor, biofuel glucose is converted to hydrogen peroxide (H 2 O 2 ) which enhances luminol-based chemiluminescence (CL), consequently driving the generation of photochemical singlet oxygen ( 1 O 2 ) via chemiluminescence resonance energy transfer. Meanwhile, hemoglobin functions as a synchronized oxygen supplier for both glucose oxidation and PDT, while also exhibiting peroxidase-like activity to produce hydroxyl radicals (·OH). Crucially, the nanoreactor keeps switching off in normal tissues, with on-demand activation in tumors through toehold-mediated strand displacement. These findings demonstrate that this nanoreactor, which is self-sufficient in light and oxygen and precise in striking tumors, presents a promising paradigm for managing highly metastatic cancers.
研究领域:
纳米技术
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生物医学工程
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癌症生物学
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治疗学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
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2024
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IF 18.5
Xiaoyi Xu,Tingting Zhou,Yu Bing,Xukun Wang,Hongtao Jiang,Zhao Song,Tong Zhang
Real-time monitoring of carbon dioxide (CO 2 ) is imperative for medical diagnosis and effective environmental preservation. Despite the formidable challenge posed by the inherent chemical inertness of CO₂ molecules, a pioneering CO 2 sensor based on MOF-804 cofunctionalized with ionic liquid (IL) and m-cresol purple (mCP) is successfully developed. By ingeniously integrating hydrogen bonding, electrostatic interactions, and hydrophobic properties within the sensitive layer, the sensor achieves a state-of-the-art sensitivity (Δf = 384 Hz), an exceptionally vast detection range spanning 400–80 000 ppm, and remarkable stability with minimal sensitivity drift even at relative humidity (RH) levels exceeding 80%. Furthermore, the inherent gasochromic property, stemming from the zwitterionic mechanism, paves the way for innovative self-sustaining CO 2 test strips and groundbreaking applications, including CO 2 tracking and CO 2 -encrypted security labeling technology. Collectively, the realization of this ultra-sensitive and robust CO 2 monitoring approach, coupled with its CO 2 -triggered visual and multifunctional capabilities, opens up novel avenues for dynamic CO 2 detection, advanced military encryption strategies, and enhanced health management systems.
研究领域:
环境科学
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材料科学
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化学工程
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传感器技术
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医疗诊断
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安全技术
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
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2024
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IF 8.3
Li Yu,Yimou Huang,Yanwei Zhao,Zhenghua Rao,Weihong Li,Zhuo Chen,Meijie Chen
Passive cooling technologies are one of the promising solutions to the global energy crisis due to no consumption of fossil fuels during operation. However, the existing radiative and evaporative coolers still have problems achieving daytime subambient cooling while maintaining evaporation over the long term. Here, we propose a self-sustained and insulated radiative/evaporative cooler (SIREC), which consists of a porous polyethylene film (P-PE) at the top, an air layer in the middle, and poly(vinyl alcohol) hydrogel with lithium bromide (PLH) at the bottom. In particular, the P-PE shows high solar reflectance (R̅solar = 0.91) and long-wave infrared transmittance (τ̅LWIR = 0.92), which reflects sunlight while enhancing the direct radiative heat transfer between outer space and PLH (ε̅LWIR = 0.96) for sky radiative cooling. In addition, the desirable vapor permeability (579 s m–1) of the P-PE also results in good compatibility with PLH for evaporative cooling (EC). Moreover, the PLH’s ability to harvest atmospheric water at night provides self-sustainment for daytime EC. The air layer between P-PE and PLH further enhances the subambient cooling performance of the SIREC. These findings indicate promising prospects for the integration of passive cooling technologies.
研究领域:
能源工程
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材料科学
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环境科学
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DOI
高影响因子
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