JOURNAL OF POLYMER RESEARCH
·
2017
·Vol. 24
·期 6
·1-11
·
IF 1.615
Xia Shan,Gao Yang,Yu Zhe,Duan Lijie,Gao Guang Hui
A novel pH-sensitive charge reversal and self-fluorescent polymeric micelle is designed and synthesized successfully. The smart micelle is prepared based on MPEG-polyurethane multi-block copolymer, which is synthesized by polycondensation, with 1, 4-bis (hydroxyethyl) piperazine (HEP) and hydroxyl-sulfamethazine (Hydroxyl-SM) as pH-sensitive molecules and fluorescein isothiocyanate (FITC) as fluorescent segment. The resulting MPEG-polyurethane multi-block copolymer is examined by 1 H–NMR, UV-vis spectra, fluorescence spectra and an acid-base titration. Moreover, the diameter, morphology and cytotoxicity of obtained polymer micelle are measured by dynamic light scattering (DLS), transmission electron microscopy (TEM) and MTT assay. The results indicate that the micelle has a small diameter of less than 200 nm and can remain unchanged within two weeks. Zeta-potential measurement shows that the negative charge of micelle can switch into positive charge as the pH value decreasing from 9.0 to 3.0. Subsequently, the fluorescence intensity decreases significantly with the reducing of pH values. The MTT assay shows the low cytotoxicity and good biocompatibility of the MPEG-polyurethane polymeric micelles. Finally, doxorubicin (DOX) is loaded into micelles to detect in vitro release behavior. The drug loaded micelles show a faster release behavior at pH 5.0 than that at pH 7.4. Therefore, the pH-sensitive charge reversal and self-fluorescent micelle can be a potential smart carrier for delivery and controlled release of protein drug.
研究领域:
生物材料
·
药物递送
·
高分子化学
·
肿瘤治疗
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2022
·
IF 5.532
Yaqi Zhu,Jian Yang,Mingwei Wang,Yangqi Meng,Rongyun Jiang,Hancheng Zhu,Duanting Yan,Chunguang Liu,Changshan Xu,Yuxue Liu
In this study, spinel-phase Zn 2 Ga 3- x Fe 3+ x Ge 0.75 O 8 ( x = 0, 0.002, 0.004, 0.02, 0.04, 0.08, 0.2) and Zn 2 Ga 2.98- x Fe 3+ x Cr 0.02 Ge 0.75 O 8 ( x = 0, 0.0004, 0.001, 0.002 0.003, 0.004) nanoparticles were prepared by one-step hydrothermal method in combination with vacuum annealing. For ZGGO: Fe 3+ nanoparticles , the near infrared (NIR) emissions (∼725 nm) originate from the 4 T 1 ( 4 G)→ 6 A 1 ( 6 S) transition of Fe 3+ occupying octahedral and tetrahedral sites of Ga 3+ . Furthermore, their afterglow time is more than 15 min and Fe 3+ doping will influence the generation of antisite defects pair ( Ga Zn 0 − Zn Ga ′ ). For Cr 3+ and Fe 3+ co-doped ZGGO nanoparticles with average size of ∼46 nm, the NIR persistent luminescence around ∼700 nm, originating from the 2 E→ 4 A 2 and 4 T 2 → 4 A 2 transitions of Cr 3+ , can be observed and the afterglow time exceeds 850 min. With the increase of Fe 3+ concentration, the afterglow intensity decreases due to the decreased number of ( Ga Zn 0 − Zn Ga ′ ) defects and deeper traps. In particular, autofluorescence-free imaging and MR imaging were successfully realized by using ZGGO: Cr 3+ , Fe 3+ 0.002 nanoparticles dispersed in the simulated biological fluid environments (H 2 O, SLS and HSA). Their clear afterglow images can be acquired within 3 min after the stoppage of the UV irradiation and their transversal relaxivity (r 2 ) is in the range of 227–140 mM −1 s −1 , which is close to the conventional Feridex T 2 agent (108 mM −1 s −1 ). Our results suggest that ZGGO: Cr 3+ , Fe 3+ nanoprobes have potential applications in the diagnosis and therapy of disease.
研究领域:
材料科学
·
医学影像学
·
生物医学工程
✓ 已验证
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2023
·Vol. 33
·期 19
·2213545
·
IF 19
Yilei Wang,Hao Liu,Hui Xie,Shaobing Zhou
Wearable flexible electronics that are derived from hydrogels inevitably undergo dehydration, and the sensing performance is highly affected because the stretchability and conductivity weaken. Monitoring the water retention rate of hydrogels as they operate without dismantling the assembled sensing device is vital for evaluating sensing performances and intervening in a timely manner to address the problem. However, relevant research is still lacking. Herein, an autofluorescent hydrogel is engineered based on clusterization-triggered emission (CTE) for wearable strain-sensing electronics that can undergo self-visualizing dehydration. The fluorescence intensity of the CTE-type autofluorescent hydrogel depends on the aggregation level of molecule clusters that are dangled on the gel networks, which is dominated by the water retention rate of the hydrogel. Thus, the dehydration process can be reflected by the fluorescent images taken in a scenario of long-term strain-sensing. This strategy helps the operator evaluate the water retention rate of the hydrogel without removing it from the assembled electronics and then quickly address the problem. In addition, this strategy is also applicable for dehydration-tolerant systems, demonstrating the versatility of the autofluorescent hydrogel. Overall, the CTE-type autofluorescent hydrogel will promote the development of high-performance and smart wearable electronics.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Fang Hao,Zhu-Ying Yan,Zhenyu Wang,Xiu-Ping Yan
The uptake of plastic particles by plants and their transport through the food chain make great risks to biota and human health. Therefore, it is important to trace plastic particles in the plant. Traditional fluorescence imaging in plants usually suffers significant autofluorescence background. Here, we report a persistent luminescence nanoplatform for autofluorescence-free imaging and quantitation of submicrometer plastic particles in plant. The nanoplatform was fabricated by doping persistent luminescence nanoparticles (PLNPs) onto polystyrene (PS) nanoparticles. Cr3+-doped zinc gallate PLNP was employed as the dopant for autofluorescence-free imaging due to its persistent luminescence nature. In addition, the Ga element in PLNP was used as a proxy to quantify the PS in the plant by inductively coupled plasma mass spectrometry (ICP-MS). Thus, the developed nanoplatform allows not only dual-mode autofluorescence-free imaging (persistent luminescence and laser-ablation ICP-MS) but also ICP-MS quantitation for tracking PS in plant. Application of this nanoplatform in a typical plant model Arabidopsis thaliana revealed that PS mainly distributed in the root (>99.45%) and translocated very limited (<0.55%) to the shoot. The developed nanoplatform has great potential for quantitative tracing of submicrometer plastic particles to investigate the environmental process and impact of plastic particles.
研究领域:
环境科学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2017
·
IF 4.233
Huiying Li,Honghao Zheng,Weijun Tong,Changyou Gao
Ionic strength-responsive microcapsules with auto-fluorescence were fabricated by incubation of poly(allylamine hydrochloride) (PAH)-doped CaCO 3 particles in triethylamine (Et 3 N), followed by core removal using HCl. Based on the combination of hydrophobic interaction and hydrogen bonding , PAH and Et 3 N formed a complex with a molar ratio of 3:1 (repeating unit of PAH: Et 3 N). The as-prepared capsules showed extraordinary stability against 1 M HCl, 1 M NaOH and 6 M urea solutions, and could swell or shrink reversibly in response to the ionic strength. Furthermore, the capsules possessed auto-fluorescence, allowing easily tracking of capsules during applications. Such interaction may be expanded to formation of stimuli-responsive multilayer films and other colloidal particles.
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2018
·Vol. 29
·期 7
·2002-2009
·
IF 2.137
Man Xi,Yang Jiang
A series of methoxypolyethylene glycol-terminated self-fluorescent polyurethane multi-block copolymers with excellent pH-responsivity, self-fluorescence, and biocompatibility are designed and synthesized. In our design, 1, 4-bis (hydroxyethyl) piperazine is chosen as a pH-responsive segment which can donate or accept protons in response to the change of environmental pH, and fluorescein isothiocyanate is used as a fluorescent dye conjugated into the micelles to offer self-fluorescence. The chemical structure of the polyurethane multi-block copolymers is characterized by nuclear magnetic resonance and Fourier transform infrared spectroscopy. The results of the acid-based titration, the fluorescence spectrometry, and the ultraviolet visible spectroscopy indicate that the polyurethane multi-block copolymers own an excellent pH-buffering capacity responded to the change of pH values and the favorable self-fluorescence property in an aqueous solution. And the ultraviolet absorption peaks of samples are strengthened with increasing of pH values, indicating that methoxypolyethylene glycol-terminated self-fluorescent polyurethane multi-block copolymer can be a pH-dependent fluorescent probe in a broad pH range. In addition, the in vitro cytotoxicity test showed that the polyurethane multi-block copolymer has low cytotoxicity and good biocompatibility, which make it a promising nanoplatform for molecular imaging, diagnosis, and treatment of disease.
研究领域:
材料科学
·
生物医学工程
·
光学成像
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2016
·Vol. 26
·期 5
·1763-1772
·
IF 1.601
Yan Dan,Deng Chun,He Yu,Ge Yili,Song Gongwu
In this paper, an autofluorencent polyethyleneimine (PEI) microspheres probe has been designed as the two channel sensor for both quantitative fluorescence and visual detection of Cu 2+ . We synthesized autofluorescent microspheres crosslinked with glutaraldehyde by controlling volume ratio between oil phase and water phase. A novel fluorescent platform for sensing Cu 2+ based on the autofluorescence quenching of PEI microspheres by Cu 2+ with a detection limit as low as 2 nM. In addition, the absorbance of PEI microspheres at 205 nm, 325 nm, 590 nm and 800 nm were sensitive to the presence of Cu 2+ . The strong chelation interaction between Cu 2+ and -NH 2 on the surface of microspheres produced the fluorescence quenching and color change of PEI microspheres which facilitated our sensing approach. We regard the developing of autofluorcent PEI microspheres as the dual-channel sensor for Cu 2+ would provide autofluorcent microspheres a future application in quantitative analysis.
研究领域:
分析化学
·
生物医学工程
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2014
·
IF 4.287
An Gong,Xuehua Ma,Lingchao Xiang,Wenzhi Ren,Zheyu Shen,Aiguo Wu
The aim of this study is to explore an improved double emulsion technology with in situ reaction of lysine (Lys) and glutaraldehyde (GA) for fabricating autofluorescent Lys-poly(lactic-co-glycolic acid)-GA (Lys-PLGA-GA) microcapsules as novel ultrasonic/fluorescent dual-modality contrast agents. Scanning electron microscope (SEM) and static light scattering (SLS) results show that 80% of the Lys-PLGA-GA microcapsules are larger than 1.0 μm and 90% of them are smaller than 8.9 μm. SEM and laser confocal scanning microscope (LCSM) data demonstrate that the structure of our Lys-PLGA-GA microcapsules is hollow. Compared with the FT-IR spectrum of PLGA microcapsules, a new peak at 1644 cm −1 in that of Lys-PLGA-GA microcapsules confirms the formed Schiff base in Lys-PLGA-GA microcapsules. LCSM images and fluorescence spectra show that our Lys-PLGA-GA microcapsules exhibit bright and stable autofluorescence without conjugation to any fluorescent agent, which can be ascribed to the n–π* transitions of the C N bonds in the formed Schiff base. Our autofluorescent Lys-PLGA-GA microcapsules might have more wide applications than traditional fluorescent dyes because their excitation and emission spectra are both broad. The fluorescence intensity can also be tuned by the feeding amount of Lys and GA. The MTT assays reveal that the autofluorescent microcapsules are biocompatible. The results of fluorescent imaging in cells and in vitro ultrasonic imaging demonstrate the feasibility of our autofluorescent Lys-PLGA-GA microcapsules as ultrasonic/fluorescent dual-modality contrast agents. This novel ultrasonic/fluorescent dual-modality contrast agent might have potential for a variety of biological and medical applications.
研究领域:
医学影像学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Yan Gao,Junpeng Shi,Xia Sun,Chen Wu,Longlong Cao,Xiaofang Luo,Jinyuan Wang,Xiao Zhang,Yun Zhang
Fluorescent immunochromatographic test strips are critical for highly sensitive, rapid detection. However, their sensitivity for detecting trace biomarkers is often affected by autofluorescence and photon absorption of the samples (nitrocellulose membrane substrates and blood chromophores). Here, we report for the first time an immunochromatographic test strip based on near-infrared (NIR) persistent luminescence nanoparticles (PLNPs) for the highly sensitive detection of the biomarker N-terminal pro-brain natriuretic peptide (NT-proBNP). NIR PLNPs exhibit long NIR persistent luminescence (PersL) properties after excitation, allowing separation of excitation and detection, thereby eliminating sample autofluorescence interference. In addition, the PersL in the NIR region greatly reduces the absorption of photons by the sample, further improving the detection sensitivity. After optimization, the test strips can detect NT-proBNP at levels as low as 15.28 pg/mL, which covers the required clinical detection range. The assay demonstrated a recovery rate of 94–101% in human serum samples, and the inter and intrabatch coefficients of variation were less than 15%. The concentrations of NT-proBNP measured in clinical samples correlated well with those of the Roche electrochemiluminescence immunoassay, which is widely used in clinical practice. This NIR PersL immunochromatographic test strip has potential applications in the highly sensitive and immediate detection of trace biomarkers.
研究领域:
生物医学工程
·
临床检验
✓ 已验证
产品关联
DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2018
·
IF 3.649
Xiubin Xu,Shaoyu Lü,Can Wu,Zhiyong Wang,Chen Feng,Na Wen,Mingzhu Liu,Xinyu Zhang,Zhen Liu,Yongqi Liu,Chunzhen Ren
We reported the facile preparation of self-fluorescent mesoporous silica nanoparticles based on a triple-role curcumin polymer shell as stimuli-responsive multifunctional nanocarriers for drug delivery. This curcumin polymer shell integrates multiple function of acting as fluorescent agents, increasing the drug loading ratio of curcumin, and controlling the open and close of the pores in a “smart” way. This novel drug nanocarrier will inspire the development of smart and self-fluorescent drug nanocarrier to achieve imaging and therapeutic goals for cancer therapy.
研究领域:
纳米医学
·
药剂学
·
肿瘤学
✓ 已验证
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
Yu Juan,Xu Chaoqun,Lu Chuanwei,Liu Qian,Wang Jifu,Chu Fuxiang
Preparation and application of sustainable polymers derived from renewable resources are of great significance. The aim of this study is to synthesize a kind of sustainable polymeric micelles from rosin and vegetable oils via atom transfer radical polymerization (ATRP) and to investigate the doxorubicin delivery properties of these micelles. Dehydroabietic acid–based poly lauryl methacrylate (DA-PLMA) with narrow PDI of 1.13 was prepared in a well-controlled process using rosin as an ATRP initiator. Thereafter, carboxylic groups were introduced to form poly methacrylic acid (PMAA) moieties in DA-PLMA polymer via acid hydrolysis. The resulted DA-PLMA-PMAA could self-assemble in water to form pH-dependent polymeric micelles with a diameter of ∼65 nm and PDI as low as 0.105. Owing to the existence of rosin, DA-PLMA-PMAA micelles also showed self-fluorescence properties. In addition, Dox-loaded micelles were prepared in aqueous solution with the drug-loading capacity as high as 16.0% and showed sustained-release characteristics. These results demonstrate great promise for designing polymeric micellar from rosin and vegetable oils.
研究领域:
材料科学
·
生物医学工程
·
药剂学
✓ 已验证
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2016
·
IF 3.42
Na Cao,Yanbao Zhao,Bin Sang,Zhihua Wang,Liuqin Cao,Lei Sun,Xueyan Zou
Porous silica nanoparticles were prepared by emulsio n -condensation route. The silica nanoparticles with diameter of 50 nm have both accessible center-radial large pore channels (19.9 nm) and small pore size of 3.5 nm. The hierarchical porous structure endows them large pore volume for loading drugs and sustained release property. The silica nanoparticles were further modified with glucose-oxidized glutathione. The formulated Schiff base and disulfide bonds render the silica nanoparticles auto-fluorescent and redox-responsive properties. The cleavage of disulfide bonds caused by reactive thiols facilitates aminomethylbenzoic acid (AMA) release. The release of drug leads to the loss of fluorescence, which would be used to monitor the drug delivery and carrier distribution.
研究领域:
材料科学
·
生物医学工程
·
药学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）