BIORESOURCE TECHNOLOGY
·
2025
·
IF 9
Yuan Li,Danchen Zhu,Linmeng Hong,Jixiang Dai,Lijun Wu
This study systematically investigated the effect of interfering with autoinducer-2 (AI-2) signaling system on controlling initial fouling attachment and irreversible components. Exposure of bacteria to the inhibitor significantly reduced the expression of the luxS gene, which resulted in a 37.3% reduction of AI-2 signaling molecule synthesis. Expression of flagellin and kinesin, which determine bacteria adhesion properties, and virulence genes was significantly down-regulated. AI-2 quorum quenching (QQ) significantly suppressed the initial biofilm growth and inhibition efficiency was positively correlated with bacteria concentration. Extracellular polymeric substances accumulation decreased by 40.8% in QQ experiment relative to the control, thus mitigating the flux decline. Polysaccharides, which constitute irreversible components, were significantly reduced by 69.3% with presence of inhibitor. AI-2 QQ altered protein and peptide structure of fouling layer, which converted irreversible fouling into reversible fouling. These findings contributed to the understanding of QQ susceptibility to initial fouling, providing a theoretical basis for engineering applications.
研究领域:
微生物学
·
环境工程
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Optical Materials Express
·
2017
·Vol. 7
·期 7
·2150-2160
·
IF 2.591
Wenhui Zhang,Yue Jiang,Yang Ding,Mingzhi Zheng,Sujuan Wu,Xubing Lu,Xinseng Gao,Qianming Wang,Guofu Zhou,Junming Liu,Michael J. Naughton,Krzysztof Kempa,Jinwei Gao
We demonstrate an environment-friendly solvent treatment that leads to a dramatic performance improvement of perovskite solar cells, with power conversion efficiency as high as 18%, with good stability. The solvent not only improves perovskite film morphology and crystallinity, but also, at proper growth conditions, leads to the formation of a self-induced textured structure on a dense layer; this increases the interface area between the perovskite active layer and the hole transport layer and thus improves photo generated current density. These results may open a new path to low-cost and high efficiency perovskite solar cells.
研究领域:
材料科学
·
能源技术
·
光伏器件
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DOI
高影响因子
CHEMISTRY OF MATERIALS
·
2020
·
IF 9.567
Xuelian Yu,Yuxuan Wu,Jiayi Cui,Yue Ran,Wei Lin,Li-Min Liu,Jinhua Ye,Yihe Zhang,Andreu Cabot
Strain engineering has become an emerging strategy to tailor the performance of nanocatalysts. However, current strained catalysts overwhelmingly rely on stresses originated at catalyst–support interfaces. Self-standing strained nanostructures are extremely challenging to produce, and thus their impact on catalytic and photocatalytic activities remains largely unknown. Herein, we propose a ligand-based colloidal strategy for the one-step growth of bended metal chalcogenide nanoplates with a built-up strain. This strategy is based on the seeded growth of strained nanostructures from sacrificial seeds which provide a proper lattice mismatch. We demonstrate this strategy for the synthesis of strained orthorhombic SnSe and SnS nanoplates from rock salt SnS seeds. We probe not only the presence of atomic distortions in the periodic lattice but also its coupling with the generation of a large density of selenium vacancies. Geometrical phase analysis evidences the formation of a strain field in self-bended SnSe nanoplates, with a maximum tensile strain up to 12%. Theoretical and experimental results further reveal that the strain-related selenium vacancies strongly affect the electronic structure of SnSe and facilitate the mobility of photogenerated charge carriers, which result in a significantly improved photoelectrochemical activity. The strategy presented here opens a new avenue for precisely tuning semiconductor functionalities via strain engineering.
研究领域:
材料科学
·
化学工程
·
纳米技术
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2019
·Vol. 42
·期 18
·2993-3001
·
IF 2.516
Jiaying Xue,Xianbin Zhu,Xiangwei Wu,Taozhong Shi,Dong Zhang,Rimao Hua
A novel dispersive liquid-liquid microextraction that combines self-induced acid-base effervescent reaction and manual shaking, coupled with ultra high performance liquid chromatography with tandem mass spectrometry was developed for simultaneous determination of ten neonicotinoid insecticides and metabolites in orange juice. An innovative aspect of this method was the utilization of the acidity of the juice for a self-reaction between acidic components contained in the juice sample and added sodium carbonate which generated carbon dioxide bubbles in situ, accelerating the analytes transfer to the extractant of 1-undecanol. The total acid content of juice sample was measured to produce the maximum amount of bubbles with minimum usage of carbonate. Manual shaking was subsequently adopted and was proven to enhance the extraction efficiency. The factors affecting the performance, including the type and the amount of the carbon dioxide source and extractant, and ionic strength were optimized. Compared with conventional methods, this approach exhibited low limits of detection (0.001–0.1 µg/L), good recoveries (86.2–103.6%), high enrichment factors (25–50), and negligible matrix effects (−12.3–13.7%). The proposed method was demonstrated to provide a rapid, practical, and environmentally friendly procedure due to no acid reagent, toxic solvent, or external energy requirement, giving rise to potential application on other high acid-content matrices.
研究领域:
分析化学
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食品科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Xiaojie Wu,Xin Wang,Jing Kang,Alideertu Dong,Ying-Wei Yang
Staphylococcus aureus (S. aureus) is a prevalent and highly virulent pathogen responsible for microbial keratitis, which can lead to stromal damage, corneal perforation, and even blindness. In the postantibiotic era, the escalating prevalence of antibiotic-resistant S. aureus has rendered conventional antibiotic treatments increasingly ineffective, particularly due to autoinducing peptide-mediated signaling. Meanwhile, antibiotics disrupt the balance of the microbiota, which can further trigger ocular complications. To address these challenges, we designed and prepared a camouflaged bacterial system, supramolecular-coated S. aureus (SMCS), which can identify and aggregate with the matching pathogenic S. aureus through autoinducing peptide-mediated signaling. Upon the addition of amantadine, competitive host–guest interactions trigger the release of the antibacterial agent, enabling efficient and selective sterilization against pathogenic S. aureus with a fourfold higher efficacy than that against beneficial bacteria. In a rat model of bacterial keratitis, the proportion of Staphylococcus is 13.71% in the cornea after SMCS treatment, which is comparable to that in the healthy cornea (7.40%). SMCS+amantadine treatment can effectively eradicate bacteria, increase the diversity and abundance of corneal microorganisms, and preserve the balance of the corneal microbiota, providing promising clinical prospects in bacterial infection treatments and bacterial-mediated bioapplications.
研究领域:
微生物学
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眼科学
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感染性疾病
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纳米医学
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分子生物学
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转化医学
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抗菌治疗
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产品关联
DOI
PubMed
高影响因子
Advanced Fiber Materials
·
2022
·Vol. 4
·期 5
·1219-1232
·
IF 12.958
Jiao Yongjie,Li Xiaojing,Chen Jinjie,Li Chaojing,Liu Laijun,Liu Xingxing,Wang Fujun,Chen Guang,Wang Lu
Fibrosis is a common problem in soft tissue regeneration, often caused by the differentiation of fibroblasts into myofibroblasts. Because of the nanoscale topology that regulates the mechanical transduction of cells, nanofibers or nanoparticles are commonly used to modulate fibroblast differentiation. However, the strength of nanofibers is insufficient, and the physiological toxicity of nanoparticles still remains to be verified. In this study, self-induced crystallization was used to construct nano-protrusions on the random and aligned polycaprolactone microfibers to regulate the behavior of fibroblasts. The results revealed that the mechanical properties of microfibers with a nanoscale topology were improved. Immunofluorescence staining manifested that nano-protrusions impeded the activation of integrins and vinculins, thereby inhibiting the nuclear transfer of Yes-associated protein, resulting in a decrease in the expression of α- smooth muscle actin. Nanoscale topology of microfibers hampered the activation of the Rho/ROCK signalling pathway. In general, we used a simple process to construct a fibrous scaffold with a micro-nano multilevel structure. This structure can hinder the transformation of fibroblasts into myofibroblasts on both random and aligned fibers, which is expected to prevent fibrosis. Graphical abstract
研究领域:
生物材料
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组织工程
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细胞生物学
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纳米技术
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产品关联
DOI
高影响因子
Computational and Structural Biotechnology Journal
·
2021
·
IF 7.271
Jinxing Liao,Danyu Shen,Long Lin,Hongjun Chen,Yajie Jin,Shan-Ho Chou,Xiao-Quan Yu,Tao Li,Guoliang Qian
Acyl-homoserine lactone (AHL) is the most studied autoinducer in gram-negative bacteria controlling infections of various pathogens. Quenching of AHL signaling by inhibiting AHL synthesis or AHL-receptor binding via small molecular chemicals or enzymatically degrading AHL is commonly used to block bacterial infections. Here, we describe a new quorum-quenching strategy that directly “acquires” bacterial genes/proteins through a defined platform. We artificially expressed a typical AHL synthase gene pcoI from the biocontrol Pseudomonas fluorescens 2P24 in the antifungal bacterium Lysobacter enzymogenes OH11 lacking AHL production. This step led to the discovery of multiple PcoI interacting protein candidates from L. enzymogenes . The individual expression of these candidate genes in 2P24 led to the identification of Le0959, which encodes leucyl aminopeptidase, an effective protein that inhibits AHL synthesis in 2P24. Therefore, we define Le0959 as LqqP ( L ysobacter q uorum- q uenching p rotein). The expression of pcoI in E. coli could produce AHL, and the introduction of lqqP into E. coli expressing pcoI could prevent the production of AHL. LqqP directly binds to PcoI, and this protein–protein binding reduced the abundance of free PcoI (capable of AHL synthesis) in vivo , thereby blocking PcoI-dependent AHL production. Overall, this study highlights the discovery of LqqP in quenching AHL quorum sensing by binding to AHL synthase via developing a previously-uncharacterized screening technique for bacterial quorum quenching.
研究领域:
微生物学
·
分子生物学
·
传染病学
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产品关联
DOI
PubMed
高影响因子
Food Science and Human Wellness
·
2023
·
IF 7
Zhuoning Cao,Zhen Liu,Guilin Zhang,Xiangzhao Mao
Quorum sensing (QS) system can dynamically control the expression of proteins along with the cell growth. The promoting period of QS system has been little focused on until now. In this study, a self-induced dynamic regulated expression (SIDRE) system was constructed in Escherichia coli . To enable the system suitable for the expression of enzymes, promoter engineering was used to obtain P luxI mutants. To test the SIDRE system, alginate lyase AL493 and esterase Est7 were used as target protein for expression. The enzyme activity of alginate lyase and esterase reached 96.38 % and 106.71 % of the control strains containing the T7 promoter. In high-density fermentation, the activity of alginate lyase expressed by the SIDRE system with P luxI (T-38C) as promoter was 4.34-fold of that expressed by the T7 promoter. Therefore, the P luxI mutants with different promoting periods and/or different strengths show great potential in both laboratory and industrial scale for protein expression.
研究领域:
分子生物学
·
生物技术
·
微生物学
·
基因工程
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产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Xueqi Lu,Mengying Sun,Yuqing Chen,Dashuai He,Yanfeng Tuo,Guangqing Mu,Yinglong Song
Fermented milk is a typically product of co-fermentation by a variety of lactic acid bacteria (LAB). Quorum sensing (QS), as an intercellular communication mechanism of bacteria, may serve as a key factor influencing the quality of fermented milk. In this study, the fermentation performance of Lactobacillus delbrueckii subsp . bulgaricus DPUL-F36 were evaluated after supplementation with auto-inducer-2 (AI-2). The protein-protein interaction network (PPIN) was used to retrieve the interactions between QS genes and four major metabolic pathway genes. The expression of luxS gene in DPUL-F36 may affect amino acid metabolism, carbohydrate metabolism and cell wall formation. The key differential metabolites were identified by metabolomics analysis. A variety of amino acids including L-Glutamic, L-Tryptophan, L-Threonine, L-Histidine and L-Glutamine were found to be up-regulated in response to the addition of AI-2. Malic acid and succinate gradually accumulate in the tricarboxylic acid cycle. The consumption of citric acid increased with the fermentation process. In terms of rheological and texture characteristics of fermented milk, addition of AI-2 could effectively improve its shear stress resistance, firmness and index of viscosity. Our research provided a theoretical basis for improving the fermentation performance of DPUL-F36 strains, and support for the screening of excellent composite starter culture.
研究领域:
微生物学
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食品科学
·
分子生物学
·
生物技术
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Mengchen Li,Bo Li,Binbin Long,Leibin Li,Yang Chen,Mingye Peng,Mengzhou Zhou
Lactobacillus plantarum R is the predominant bacterium widely used in food fermentation. However, it has a low tolerance to ethanol during fermentation, which restricts its broader application in the food industry. Although biofilm formation enhances bacterial resistance to various stresses, regulating biofilm formation in L. plantarum R remains challenging. In this study, we established a pathway for producing autoinducer 2 (AI-2), a quorum-sensing (QS) signaling molecule, by heterologously expressing pfs and LuxS in L. plantarum R. This approach aimed to modulate cell membrane structure and improve bacterial ethanol resistance. The findings showed that exogenous AI-2 significantly enhanced the growth of L. plantarum R under high-ethanol concentration and improved its biofilm formation at different ethanol concentrations. Furthermore, a multi-omics analysis revealed potential mechanisms by which AI-2 improved the tolerance of L. plantarum R to ethanol. Notably, the upregulation of the crr gene, a component of the phosphotransferase system (PTS), enhanced biofilm formation in response to ethanol stress. In addition, differential metabolites enriched in cysteine and methionine metabolic pathways further supported the role of QS in enhancing the tolerance of L. plantarum R to ethanol. These findings lay the groundwork for enhancing the fermentation performance of strains applied in the food fermentation industry, presenting a novel method for expanding the utilization of L. plantarum R in high-ethanol fermentation.
研究领域:
微生物学
·
食品生物技术
·
分子生物学
·
系统生物学
·
合成生物学
·
发酵技术
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产品关联
DOI
高影响因子
WATER RESEARCH
·
2020
·
IF 9.13
Kun Li,Jin Qian,Peifang Wang,Chao Wang,Bianhe Lu,Wen Jin,Xixian He,Sijing Tang,Chao Zhang,Pan Gao
Most of the current studies on the toxicology of pristine nanoparticles (NPs) are environmentally irrelevant, because their ‘‘aging’’ process accompanied by the physicochemical transformation is inevitable in the environment. Considering aging phenomenon will gain a better understanding of the toxicity and fate of NPs in the environment. Here, we focused on the physicochemical transformation of anatase-NPs (TiO 2 -A) and rutile-NPs (TiO 2 -R) after 90 days of aging and investigated the responses of freshwater biofilm formation to the stress changes of naturally aged TiO 2 -NPs (aTiO 2 -NPs). We found that after aging, the TiO 2 -NPs underwent sophisticated physicochemical transformations in the original morphology and microstructure owing to organic and crystal salts inclusions, such as energy band changes and the formation of Ti 3+ on the NPs surfaces. These comprehensive transformations increased the stability of NPs in the exposed suspension. However, the physicochemical transformations were crystal-forms-dependent, and aging did not change the crystal structure and crystallinity . Interestingly, compared to pristine NPs, aTiO 2 -NPs showed much lower cytotoxicity and had the weaker ability to promote or inhibit the biofilm formation (p < 0.05) owing to the passivation of photoactivity caused by the comprehensive effect of the inclusions, especially for aTiO 2 -A. Regardless of aging or not of crystal forms, responses of biofilm formation were exposure-concentration-dependent, namely low concentration promotion (0.1 mg/L) and high concentration inhibition (10 mg/L), e.g., role transition of the pioneers (algae or bacteria) in initial colonization, extracellular polymeric substances (EPS) secretion and compositions of development stages with polysaccharide (PS)-rich and protein (PRO)-rich stages, and biomass and cell activity at different depths of mature biofilms. The reactive oxygen species (ROS) induced by TiO 2 -NPs showed typical hormesis. The changing trends of the autoinducers (c-di-GMP and quorum sensing signals including AHL and AI-2) were highly consistent with the growth stages of biofilms and were stimulated or suppressed by TiO 2 -NPs. The NPs crystal-dependently changed the microorganism community structures, while the UPGMA clustering of bacteria was based on the growth stages of the biofilms. The toxic mechanisms revealed that photoactivity and nanoscale retention of particles are the main reasons for the differences in the ecological stress capacity of four kinds of TiO 2 -NPs. Aging reduced characteristic differences of two pristine NPs and even reversed their relative stresses levels (p > 0.05). However, the toxicity of high-concentration aTiO 2 -NPs (10 mg/L) remained serious in a water environment. This study provides a better understanding for the water environmental risks evaluation and policy control of nanoparticles, that is, the effect of time aging has to be considered.
研究领域:
环境科学
·
纳米技术
·
生态毒理学
·
微生物学
·
水生生态学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Energy Chemistry
·
2025
·
IF 14.9
Sinan Zheng,Yang Wang,Bin Luo,Leilei Sun,Guosheng Duan,Zhean Bao,Li Gong,Jingyun Huang,Zhizhen Ye
Benefiting from features such as high-voltage platforms, superior energy density, and cost-effectiveness, Zinc-bromine flow batteries (ZBFBs) demonstrate significant potential for large-scale energy storage applications. However, their practical implementation faces critical challenges: sluggish Br 2 /Br − redox reactions and severe shuttle effects, which lead to diminished energy efficiency and self-discharge. Here, we develop an innovative electrochemical self-induced catalyst gel encapsulation strategy. Under electrochemical driving, the poly(3,4-ethylenedioxythiophene): poly(styrenesulfonate) (PEDOT:PSS, abbreviated as PES) catalyst spontaneously undergoes gelation and coating on carbon fibers, thereby forming a PES@C composite cathode with enhanced bromine conversion reversibility. This innovative strategy effectively addresses three critical challenges in existing ZBFBs: shuttle effect, low bromine utilization, and poor energy efficiency. PES@C with sulfur sites and C–O-C group enable efficient bromine/poly-bromide anchoring through strong polar interactions, effectively suppressing shuttling effects. Furthermore, the abundant active sites exhibit exceptional catalytic activity towards Br 2 /Br − redox reactions. This synergistic adsorption-catalysis mechanism significantly enhances the electrochemical performance of ZBFBs. The ZBFBs with PES@C achieve stable operation under ultrahigh current density (200 mA cm −2 ) and high areal capacity (40 mA h cm −2 ), delivering outstanding voltage efficiency (84.91%) and energy efficiency (81.78%). Notably, the proposed strategy demonstrates universal applicability for performance enhancement in various bromine-based battery systems, providing an efficient pathway to improve energy efficiency and cycling durability in bromine-based flow batteries.
研究领域:
储能技术
·
电化学
·
材料科学
·
催化
·
可再生能源
·
电池技术
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）