Frontiers in Pharmacology
·
2022
·
IF 5.988
Dong Zhen,Tang Shu-sheng,Ma Xiao-lan,Tan Bin,Tang Zhao-shan,Li Chang-hong,Yang Zi-hui,Zeng Jian-guo
The protopine alkaloids are widely distributed within the opium poppy family and have a wide range of pharmacological effects. MPTA is a product of the protopine total alkaloids extracted from the Macleaya cordata (Willd.) R. Br. Previously, we reported good anti-inflammatory activity of MPTA as well as oral acute and sub-chronic toxicity studies in rats. In order to perform a systematic toxicological safety assessment of MPTA, oral acute toxicity, genotoxicity (bone marrow cell chromosome aberration test, sperm abnormality test, bone marrow cell micronucleus test, and rat teratogenicity test), and chronic toxicity in mice were performed in this study. In the oral acute toxicity test, the LD50 in ICR mice was 481.99 mg/kg, with 95% confidence limits ranging from 404.27 to 574.70 mg/kg. All three mutagenicity tests tested negative in the range of 60.25–241.00 mg/kg. The results of the teratogenicity test in rats showed no reproductive or embryonic developmental toxicity at only 7.53 mg/kg, which can be considered as a no observed effect level (NOEL) for the teratogenicity test. Therefore, MPTA is safe for use at the doses tested, but attention should be paid to the potential risk to pregnant animals and the safety evaluation and toxicity mechanisms in target animals should be further investigated.
研究领域:
毒理学
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药理学
·
遗传学
✓ 已验证
DOI
PubMed
高影响因子
Biomicrofluidics
·
2015
·Vol. 9
·期 2
·
IF 3.357
Li Yinbao,Yang Xiujuan,Chen Zuanguang,Zhang Beibei,Pan Jianbin,Li Xinchun,Yang Fan,Sun Duanping
Investigations were conducted to determine acute effects of Pb 2+ and Cu 2+ presented individually and collectively on zebrafish embryos. Aquatic safety testing requires a cheap, fast, and highly efficient platform for real-time evaluation of single and mixture of metal toxicity. In this study, we have developed a microfluidic system for phenotype-based evaluation of toxic effects of Pb 2+ and Cu 2+ using zebrafish ( Danio rerio ) embryos. The microfluidic chip is composed of a disc-shaped concentration gradient generator and 24 culture chambers, which can generate one blank solution, seven mixture concentrations, and eight single concentrations for each metal solution, thus enabling the assessment of zebrafish embryos. To test the accuracy of this new chip platform, we have examined the toxicity and teratogenicity of Pb 2+ and Cu 2+ on embryos. The individual and combined impact of Pb 2+ and Cu 2+ on zebrafish embryonic development was quantitatively assessed by recording a series of physiological indicators, such as spontaneous motion at 22 hours post fertilization (hpf), mortality at 24 hpf, heartbeat and body length at 96 hpf, etc. It was found that Pb 2+ or Cu 2+ could induce deformity and cardiovascular toxicity in zebrafish embryos and the mixture could induce more severe toxicity. This chip is a multiplexed testing apparatus that allows for the examination of toxicity and teratogenicity for substances and it also can be used as a potentially cost-effective and rapid aquatic safety assessment tool.
研究领域:
毒理学
·
环境科学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
BIOLOGICAL TRACE ELEMENT RESEARCH
·
2021
·Vol. 200
·期 9
·4027-4034
·
IF 3.738
Gao Yuqiu,Wen Pingjing,Chen Huafeng,Wei Yujia,Cui Haichen,Ma Jinfeng,Li Jinyue,Qin Guangqiu
Aluminum oxide nanoparticles (Al 2 O 3 NPs) are one class of widely used nanomaterials. However, the teratogenicity toxicity of Al 2 O 3 NPs in mammal remains poorly understood. This study was aimed to evaluate the teratogenicity of Al 2 O 3 NPs in Sprague Dawley (SD) rats by gavage and to compare the effects of Al 2 O 3 NPs to those of equivalent dose of microscale aluminum oxide (bulk Al 2 O 3 ). Sixty pregnant rats were randomly divided into 5 groups and treated with 100 and 200 mg/kg body weight (bw) Al 2 O 3 NPs (30 nm), 200 mg/kg bulk Al 2 O 3 , deionized water (as the negative control), and 300 mg/kg aspirin (as the positive control). Rats were exposed daily by oral gavage from the 7 th day of gestation for 10 consecutive days and sacrificed on the 20 th day of gestation. Results of the study showed that there were no significant effects of Al 2 O 3 NPs on pregnant rats (clinical signs, body weight, food consumption, ovary and uterus weight, number of corpora lutea) and fetuses (body weight, sex, body length, tail length, skeletal and visceral development). Under the experimental conditions of the present study, 10 consecutive days of repeated oral administration of Al 2 O 3 NPs at doses of up to 200 mg/kg/day did not induce any treatment-related teratogenicity in SD rats. Accordingly, the NOAEL was determined to be 200 mg/kg Al 2 O 3 NPs (106 mg Al/kg bw/day) in rats. The teratogenic effects of Al 2 O 3 NPs in rats were comparable to those of the bulk Al 2 O 3 of same doses (200 mg/kg).
研究领域:
毒理学
·
纳米生物学
·
生殖医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2024
·
IF 4.4
Wu Lirong,Wang Jieyi,Lei Jing,Ge Kun,Qu Chun,Liu Jiajian,Huang Fengjie,Sun Dongnan,Chao Xiaowen,Chen Tianlu,Zhao Aihua,Jia Wei,Zheng Xiaojiao,Xie Guoxiang
Background: Porcine bile powder (PBP) is a traditional Chinese medicine that has been used for centuries in various therapeutic applications. However, PBP has not previously undergone comprehensive component analysis and not been evaluated for safety through standard in vivo toxicological studies.Methods: In our study, we characterized the component of PBP by liquid chromatography-mass spectrometry. The acute and subchronic oral toxicity, genotoxicity, and teratogenicity studies of PBP were designed and conducted in Kunming mice and Sprague-Dawley (SD) rats.Results: The chemical analysis of PBP showed that the main components of PBP were bile acids (BAs), especially glycochenodeoxycholic acid. There were no signs of toxicity observed in the acute oral test and the subchronic test. In the genotoxicity tests, no positive results were observed in the bacterial reverse mutation test. Additionally, in the mammalian micronucleus test and mouse spermatocyte chromosomal aberration test, no abnormal chromosomes were observed. In the teratogenicity test, no abnormal fetal development was observed.Conclusion: Our findings demonstrate that PBP, composed mainly of BAs, is non-toxic and safe based on the conditions tested in this study.
研究领域:
药理学
·
毒理学
·
传统中药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2022
·
IF 11.357
Xiaolin Fan,Jilong Guo,Xiaojing Jia,Xingtai Mao,Ying Zhou,Yue Wang,Xuan Guo,Jiashu Shen,Narma Huai,Kailun Zhang,Abida Abdusalam,Hongxia Hu,Hua Zhu,Chengxia Jia,Lan Cheng,Xiqing Li,Zhaobin Zhang
Fluorene-9-bisphenol (BHPF), a bisphenol A (BPA) substitute, has been increasingly used as a material in syntheses of polymers that are widely used in road markings, artificial tracks, coating floors, building paints, etc., increasing the likelihood of BHPF contamination in the aquatic environment due to its release from the products. However, to date, it is unknown whether it may have actual impacts on fish in real environments. In this study, a 105-day exposure experiment of BHPF at various concentrations (0.01, 0.1, 1, and 10 μg/L) on Chinese medaka (Oryzias sinensis) was performed under laboratory conditions and found decreased fecundity, such as lower egg qualities and quantities, retarded oogenesis, and atretic follicles in the fish and deformed eyes and bodies in its F1 generation. Toxico-transcriptome analyses showed that estrogen-responsive genes were significantly suppressed by BHPF, indicating that antagonist properties of BHPF on estrogen receptors might be causes for the decreased fecundity. Field investigations (Beijing) demonstrated that BHPF was detectable in 60% surface waters, with a mean concentration of 10.49 ± 6.33 ng/L, by gas chromatography–mass spectrometry, and similar effects in wild Chinese medaka were also observed, some of which the parameters were found to be obviously correlated with the BHPF levels in corresponding waters.
研究领域:
环境科学
·
毒理学
·
水生生态学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY
·
2021
·
IF 1.437
Shanshan Wang,Hufei Wang,Yanqiu Chen,Jilai Liu,Xiaozhen He,Da Huang,Yuanzi Wu,Yonghai Chen,Zuquan Weng
Human lymphocytes as well as zebrafish embryos were used to study the toxicity of acrylamide, and the intervention effects of the dietary antioxidants (-)-epigallocatechin gallate and curcumin have been examined. The alkaline comet assay revealed that acrylamide (1, 5, 10 mmol/L) had dose-response effects on basic DNA damage and oxidative DNA damage in human lymphocytes. Exposure of zebrafish embryos to acrylamide resulted in decrease of hatching rate, and in increases of mortality and teratogenicity. (-)-Epigallocatechin gallate and curcumin reduced DNA damage and protected zebrafish embryos in the growth and development.
研究领域:
毒理学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Tungsten
·
2023
·
IF 6.6
Huang Yu-Lan,Zhang Bing,Li Yu-Lian,Wang Li,Dong Le,Li Jian
Due to its properties of mutagenic, teratogenic, and carcinogenic, the detection of furazolidone (FZD) in aquaculture is of great importance for food safety and human health. In this study, molecularly imprinted films modified with carboxylated multi-walled carbon nanotube-phosphomolybdic acid composite were used to fabricate an electrochemical sensor for the determination of FZD. The nanocomposites were characterized using infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction. The electrochemical characteristics of the modified electrodes were examined using electrochemical impedance spectroscopy, cyclic voltammetry, and differential pulse voltammetry. The sensor exhibited exceptional catalytic performance. The calibration curves were acquired in the concentration range of 6 nmol·L −1 to 0.6 μmol·L −1 , with a limit of detection of 3.38 nmol·L −1 . Additionally, the sensor proved successful in recognizing FZD in shrimp samples with satisfactory recoveries and precision. The method provides a strategy to construct a molecularly imprinted electrochemical sensing platform using nanomaterials, which has great promise in the field of food safety.
研究领域:
分析化学
·
纳米技术
·
食品安全
·
电化学
·
传感器
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Jun Chen,Boding Zhang,Bingxing Wang,Chengxing Cui,Songlin Wang,Jichao Wang,Wenlong Zhang
With high toxicity, carcinogenicity and teratogenicity, quinoline has been threatening human health and ecological environment. Herein, Fe-Co-Ni-P/g-C 3 N 4 particles are developed as electrochemical reactors to form a three-dimensional catalytic particle electrode system (3D-CPE) for efficient removal of quinoline from wastewater by electro-Fenton (EF) oxidation process. with reaction time of 30 min, the particles dosage of 50 g/L, pH of 3, conductivity of 11.5 ms/cm, and current density of 37.04 mA/cm 2 , the chemical oxygen demand (COD) removal rate can up to 90.95%. In addition, electron paramagnetic response (EPR) and radical scavenging tests are performed to determine the hydroxyl radicals of electrochemical processes produced. The degradation products are analyzed by high performance liquid chromatography-mass spectrometry (HPLC-MS). Finally, through theoretical calculation and analysis of degradation products, a reasonable degradation mechanism is proposed.
研究领域:
环境科学
·
化学工程
·
水处理
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）