JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Hui-Jun Fu,Zi-Jian Chen,Hong Wang,Lin Luo,Yu Wang,Ri-Ming Huang,Zhen-Lin Xu,Bruce Hammock
Ethyl carbamate is a group of 2A carcinogen ubiquitously existed in fermented foods. The monitoring of its residues was important for evaluating the potential risk to human beings. Immunoassays with good accuracy and simplicity are great analytical tools for small molecule contaminants. However, it is typically confined in a competitive mode for small molecules with drawback of the sensitivity curbing. In this work, three different phages displayed peptides with capability of identifying the xanthyl ethyl carbamate immunocomplex were isolated from phage library. The binding mechanism of peptides and immunocomplex was studied by computer-assisted simulation. Results indicated that the xanthydrol group of xanthyl ethyl carbamate and the Asn-32 and Asn-92 residues of the antibody light chain were mainly responsible for binding. Simultaneously, a sensitive non-competitive immunoassay for detecting ethyl carbamate in wine samples was developed. The established method exhibited a limit of detection of 5.4 ng/mL and a linear range from 8.7 ng/mL to 32 ng/mL for wine samples. In comparison with the conventional competitive immunoassay, the sensitivity of the proposed non-competitive immunoassay was improved by 17-fold. The results of the immunoassay were validated by a standard ultra-performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry , which illustrated good reliability of the proposed assay.
研究领域:
食品安全
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分析化学
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生物信息学
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Qian-Da Xu,Zhi-Qiang Zhou,Zan Jing,Qiang He,Qun Sun,Wei-Cai Zeng
Effect of pine needle extract from Cedrus deodara (PNE) on the quality and safety of Chinese traditional smoked bacon was observed, and its action mechanism was further investigated. With the treatment of PNE, the quality properties of smoked bacon, including moisture content, pH and titratable acidity values, color, texture and volatile compositions, were all ameliorated. Meanwhile, the peroxide value (POV), thiobarbituric acid value (TBARS) and nitrite content of smoked bacon with PNE decreased. In addition, the content of N -nitrosodimethylamine in smoked bacon gradually decreased with the increased concentration of PNE. Moreover, the heavy polycyclic aromatic hydrocarbons (PAHs) were not detected in smoked bacon with PNE, while the types and contents of light PAHs in smoked bacon were both reduced by PNE. With the measurement of fluorescence spectroscopy and circular dichroism spectroscopy , the secondary and tertiary structures of myofibrillar protein in smoked bacon were both changed though the treatment of PNE. Furthermore, according to the analysis of molecular docking, hydrogen bond was the main molecular force between PNE and meat proteins in smoked bacon. All present results suggested that PNE had the potential value to improve the quality and safety of smoked bacon in food industry.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Kun Ge,Yuling Hu,Gongke Li
Carcinogens in food samples show great potential threat to human health due to their wide distribution and high carcinogenicity . In this work, branched AuCu nanoalloy doped mesoporous graphitic carbon nitride hybrid membrane (mpg-C 3 N 4 /AuCu) was fabricated for SERS analysis of carcinogens including benzidine and zearalenone in food. The AuCu was in-situ grown on mpg-C 3 N 4 to form mpg-C 3 N 4 /AuCu composites. The as-fabricated mpg-C 3 N 4 /AuCu membrane can effectively combined synergistic effect of localized surface plasmon resonance properties of branched AuCu nanoalloy and semiconductor characteristics of mpg-C 3 N 4 . The limit of detection for crystal violet is 1.0 ng/L with enhancement factor of 3.7 × 10 8 . The mechanism of high SERS activity of mpg-C 3 N 4 /AuCu membrane was investigated by density functional theory simulations. The mpg-C 3 N 4 /AuCu membrane was used for direct determination of benzidine, and indirect determination of zearalenone with 3,3′,5,5′-tetramethylbenzidine as markers in food. The limits of detection of SERS method were 0.14 and 0.03 μg/L for benzidine and zearalenone, respectively. It provides a new strategy for design and fabrication of high-quality SERS substrates for carcinogens analysis.
研究领域:
材料科学
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分析化学
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食品安全
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Ruosi Fang,Shuhui Wu,Ting Xia,Jingjin Hu,Jiamin He,Gongnian Xiao
Ethyl carbamate (EC), a potential carcinogen, is spontaneously formed from urea and ethanol during Huangjiu fermentation, and urea is derived from arginine (Arg) degradation by Saccharomyces cerevisiae . Previous studies have shown that BTN2 influences Arg uptake and metabolism. In this study, we conducted a comprehensive analysis of the variation of EC, major metabolites, enzyme activities, volatile flavor compounds, amino acids, and general quality within the Arg metabolism pathway during Huangjiu fermentation by wild type, BTN2 Δ, and BTN2 ↑ strains. The results indicated that BTN2 knock out significantly reduced EC, urea, and citrulline by 12.28, 15.6, and 20.18%, respectively. Furthermore, flavors and amino acids among the three groups exhibited minimal variation, resulting in insignificant differences in the sensory evaluation. The primary flavors identified were ethyl lactate and isoamyl alcohol. Cysteine, Arg, and glutamine contributed significantly to the overall flavor profile. These findings suggest that Btn2p is a potential target for the reduction of EC.
研究领域:
食品科学
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微生物学
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生物化学
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生物技术
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分子生物学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Qiuyi Lin,Haolin Zhang,Xiaomei Lv,Ruiwei Xie,Bing-Huei Chen,Yu-Wen Lai,Lei Chen,Hui Teng,Hui Cao
Polycyclic aromatic hydrocarbons (PAHs), prominent carcinogens formed during food processing, pose health risks through long-term consumption. This study focuses on 16 priority PAHs in the European Union, investigating their formation during pyrolysis. Glucose, amino acids and fatty acids are important food nutrients. To further explore whether these nutrients in food form PAHs during heating, a single chemical model method was used to heat these nutrients respectively, and GC–MS/MS was used to identify and quantify the obtained components. Glucose is the most basic nutrient in food, so the influence of water, pH, temperature and other factors on the formation of PAHs was studied in the glucose model. At the same time, the models of amino acids and fatty acids were used to assist in improving the entire nutrient research system. According to our results, some previously reported mechanisms of PAHs formation by fatty acids heating were confirmed. In addition, glucose and amino acids could also produce many PAHs after heating, and some conclusions were improved by comparing the intermediates of PAHs from three types of nutrients.
研究领域:
食品科学
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化学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 9
·1164-1171
·
IF 2.896
Minglei Lu,Qiyi He,Yingying Zhong,Junkang Pan,Zhiting Lao,Mingxia Lin,Tiantian Wang,Xiping Cui,Jinlong Ding,Suqing Zhao
Progesterone (P4) belongs to a factor that affects stress response and is a potential carcinogen, and saliva levels are expected to be a standard measurement for clinical diagnosis. In this study, a new type of nanoflower with both recognition functionality and catalytic substrate ability was prepared by copper phosphate, Pt/IrO2 nanocomposites (Pt/IrO2 NPs), streptavidin (SA) and horseradish peroxidase (HRP) via a one-pot co-precipitation strategy. Due to the enhanced catalytic activity and stability of Pt/IrO2@SA@HRP nanoflowers, we developed a powerful and sensitive multiple-catalysis ELISA to monitor progesterone in saliva. Multiple-catalysis ELISA based on a specific antibody and Pt/IrO2@SA@HRP nanoflowers exhibited a linear interval range from 0.217 ng mL−1 to 7.934 ng mL−1. The median inhibitory concentration (IC50) for progesterone is 1.311 ng mL−1 and the limit of detection (LOD = IC10) is 0.076 ng mL−1 in the proposed method. Satisfactory recoveries were in a range of 79.6–107% with an acceptable coefficient of variation (below 10.6%). Results of the multiple-catalysis ELISA and LC-MS/MS had a good coincidence. Our result unraveled that multiple-catalysis ELISA is a potentially serviceable tool for the detection of progesterone in saliva.
研究领域:
分析化学
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生物化学
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纳米技术
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临床诊断
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产品关联
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2023
·
IF 4.3
Xin Wang,Guangyun Ran,Yufen Liao,Bowen Gong,Chutian Wu,Rong Tan,Ying Liu,Shiyu Zhang,Ying Peng,Weiwei Li,Jiang Zheng
Safrole (SFL) is an IARC class 2B carcinogen. To better understand the mechanism involved in SFL toxicity, we explored the potential interactions between SFL metabolites and RNA. Three guanosine adducts (G1-G3), two adenosine adducts (A1-A2), and two cytosine adducts (C1–C2) were detected by LC-MS/MS in mouse liver S9 incubations, cultured mouse primary hepatocytes, and liver tissues of mice after exposure to SFL. These adducts were chemically synthesized, and one of the guanosine adducts was structurally characterized by 1 H-NMR. Studies in vitro and in vivo showed that SFL was oxidized by cytochrome P450 enzymes to the corresponding 1′-hydroxyl metabolite which was further metabolized by sulfotransferases to form allylic sulfate esters. The formed reactive intermediate(s) subsequently reacted with bases of RNA, leading to RNA adduction, which could play a partial role in the toxicities of SFL through the alteration of RNA biochemical properties and interruption of RNA functions.
研究领域:
毒理学
·
药理学
·
分子生物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Chunjian Wu,Li Wang,He Li,Shujuan Yu
The presence of potential carcinogen 4(5)-Methylimidazole (4-MeI) in foods causes much concern, stressing the need of a sensitive determination means. Here, we proposed a high-sensitivity method to determine 4-MeI in biscuits using dansyl chloride derivatization and disperse liquid-liquid micro-extraction (DLLME) followed by a liquid chromatography-ion trap mass spectrometry (LC-IT-MS) analysis. This developed method was subsequently compared to the solid phase extraction combining with liquid chromatography-triple quadrupole mass spectrometry (SPE-LC-QqQ-MS) method. The optimized derivatization conditions were 30 °C and 10 min. Results suggested that the column retention time (RT) was significantly extended, and the MS signal response of 4-MeI-dansyl derivative was also amplified. The developed DLLME-LC-IT-MS method in 4-MeI determination provided satisfactory linearity in a range of 0.5–300 ng/mL (R 2 > 0.9991), inter-day accuracy (87-102%) and precision (%RSD ≤ 13.6%) with the quantification limit of 0.2 ng/mL, which obtained similar results comparing to the conventional SPE-LC-QqQ-MS method.
研究领域:
分析化学
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食品科学
·
质谱分析
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yang Yang,Yifan Li,Qinhui Xie,Bo Jiang,Junbo Li,Yunfei Xie,Wei Ji
Biomimetic cytochrome P450 for chemical activation of environmental carcinogens is an efficient in vitro model for evaluating their mutagenicity and ultimately acquiring the metabolites that cannot be easily accessed by conventional routes of organic synthesis. Different kinds of mutagen derived from polyaromatic hydrocarbons (PAHs) by metalloporphyrin/oxidant model systems have been reported, but the underlying molecular mechanisms are poorly understood. Herein, we have for the first time demonstrated an effective surface-enhanced Raman scattering (SERS) protocol to study the dynamics and biomimetic metabolic behaviors of pyrene (Pyr) in the presence of various oxygen donors. Quantitative information on the relative concentration of Pyr and its metabolites in the biomimetic system can be extracted from the SERS spectra. On the basis of our results, we conclude that the oxidative metabolism of Pyr is highly influenced by the types and concentrations of oxygen donors, leading to the formation of 1-hydroxypyrene and dioxygenated products. Besides, the addition of an appropriate amount of an organic solvent can promote the formation of secondary oxidation products. These results offer valuable insights into the dynamics of PAHs metabolism and the regulation of their metabolic pathways in biomimetic activation. In comparison to traditional liquid chromatography–mass spectrometry, the present SERS approach is more suitable for high-throughput evaluation of the metabolic process and kinetics of PAHs. We anticipate that this approach will enable a more general and comprehensive tracking of metabolic dynamics and molecular mechanisms involved in the biomimetic activation of other xenobiotics, such as procarcinogens, promutagens, and drugs.
研究领域:
环境科学
·
分析化学
·
生物化学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）