JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2019
·Vol. 99
·期 7
·3548-3554
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IF 2.422
Wen Nie,Kezhou Cai,Yuzhu Li,Zehui Tu,Bing Hu,Cunliu Zhou,Conggui Chen,Shaotong Jiang
BACKGROUND The characteristics of carcinogenic polycyclic aromatic hydrocarbons (PAHs) produced from various fatty acids, as important components of fats and oils, at high temperature are still little known. The reason is because the existing data are from experiments conducted in complex food systems. In this study, 12 PAHs produced from nine fatty acids, representing saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs), were investigated in a model system heated at 98, 165 and 240 °C. RESULTS SFAs can with difficulty be pyrolyzed to generate PAHs at 98 °C, but small amounts of PAHs were determined in MUFAs (44.97 µg kg −1 ) and PUFAs (177.73 µg kg −1 ). When the temperature reached 165 °C, there were totals of 27.59, 142.8 and 449.68 µg kg −1 PAHs assayed in SFAs, MUFAs and PUFAs, respectively. The amounts of PAHs generated from SFAs, MUFAs and PUFAs at 240 °C were higher when compared with those of the 165 °C group ( P < 0.05). With an increase of heating temperature, the proportion of PAHs with four to five rings increased. Under the same heating conditions, the concentration of PAHs in fatty acids increased with an increase in the number of double bonds. CONCLUSIONS More PAHs, especially carcinogenic ones with four to five rings, will be produced in fatty acids heated at higher temperature. The content of PAHs generated from fatty acids increased with an increase in the number of double bonds. This study will increase the understanding of the production characteristics of PAHs from various fatty acids under heating condition. © 2019 Society of Chemical Industry
研究领域:
食品科学
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毒理学
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化学
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DOI
PubMed
高影响因子
MOLECULAR CARCINOGENESIS
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2024
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IF 3
Junyi Jin,Yihui Wang,Yaoyuan Hu
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide. STAM binding protein-like 1 (STAMBPL1), a key member of the COP9 signalosome subunit 5/serine protease 27/proteasome 26S subunit non-ATPase 7 (JAMM) family, is closely associated with tumor development. In this work, data from GSE101728 and GSE84402 chips were analyzed, and STAMBPL1 was selected as the target factor. This study aimed to reveal the potential function of STAMBPL1 in HCC. Clinical results showed that STAMBPL1 was significantly increased in tumor tissues of HCC patients, and its expression was strongly associated with tumor size and TNM stage. Furthermore, STAMBPL1-overexpressed Hep3B2.1-7 cell line or STAMBPL1-silenced SNU-182 cell line were established using lentivirus carrying cDNA encoding STAMBPL1 mRNA or shRNA targeting STAMBPL1, respectively. STAMBPL1-overexpressed cells exhibited a pronounced enhancement of proliferation in vitro and in vivo. Exogenous expression of STAMBPL1 increased the percentage of cells in the S phase and upregulated the expressions of CyclinD1 and Survivin. As expected, STAMBPL1 knockdown exhibited completely opposite effects, resulting in impaired tumorigenicity in vitro and in vivo. Mechanistically, STAMBPL1 activated Wnt/β-catenin pathway and increased the expression of downstream cancer-promoting genes. Interestingly, we found that STAMBPL1 was transcriptionally regulated by sterol regulatory element-binding protein 1 (SREBP1), a modulator of lipid metabolism, as evidenced by luciferase reporter and chromatin-immunoprecipitation (Ch-IP) assays. Notably, STAMBPL1 overexpression increased lipid accumulation in HCC cells and xenograft tumors. Totally our findings suggest that STAMBPL1 plays a vital role in the tumorigenicity of HCC cells. Modulation of Wnt/β-catenin and lipid metabolism may contribute to its pro-cancer effects. STAMBPL1 may serve as a therapeutic target of HCC.
研究领域:
肿瘤学
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分子生物学
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细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2018
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IF 3.412
Yukun Feng,Saide Wang,Hui Wang,Ying Peng,Jiang Zheng
Methyleugenol (ME), a natural ingredient of several herbs and spices used in the human diet, is hepatocarcinogenic in rodents. Following metabolic activation to the reactive carbocation intermediate, ME can bind covalently to DNA, which is directly associated with its carcinogenicity. In this work, a non-invasive approach to determine ME exposure was established by monitoring the urinary N6-(methylisoeugenol-3′-yl)-2′-deoxyadenosine (ME-dA) adduct. The developed method entails liquid–liquid extraction enrichment of urinary ME-dA, incorporation of deuterated ME-dA as an internal standard, and analysis by liquid chromatography coupled tandem mass spectrometry. Male rats (10–12 weeks, 180–200 g) were treated (p.o.) with ME, and ME-dA was excreted in urine in a dose- and time-dependent manner. The non-invasive approach enabled us to successfully determine exposure to ME-containing herbs and spices. These results suggest that ME-dA can potentially serve as an effective biomarker of ME exposure in rats. It is expected that the developed approach of detecting urinary ME-dA will facilitate the investigation of ME carcinogenesis.
研究领域:
毒理学
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分析化学
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癌症研究
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2021
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IF 6.291
Luyao Li,Mei Zhou,Biyun Chen,Qin Wang,Shuya Pan,Yaxuan Hou,Jiamin Xia,Xue Zhou
Cadmium is a carcinogenic heavy metal that poses a severe threat to human beings. The underlying mechanism, however, remains elusive. N6-methyladenosine (m6A) is the most abundant post-transcriptional modification in mRNA that regulates RNA metabolism. Emerging evidence shows that m6A is involved in the pathogenesis of various cancers. In this study, human bronchial epithelial BEAS-2B cells were transformed by exposing to 2 μM of cadmium for 20 weeks to investigate the role of m6A in cadmium carcinogenesis. We found the level of m6A in mRNA was significantly decreased in cadmium-transformed BEAS-2B cells, and this change was regulated by m6A demethylase ALKBH5. ALKBH5 was significantly upregulated in the middle and late stages of cell transformation at week 8, 12, 16 and 20. Knockdown of ALKBH5 in cadmium-transformed cells alleviated cell proliferation, migration, invasion, and anchorage-independent growth, but co-transfection with ALKBH5 siRNA and PTEN siRNA restored the inhibitory effects of ALKBH5 knockdown on those transformation properties. ALKBH5 decreased the m6A level of PTEN mRNA, resulting in its instability and reduction of PTEN protein expression. These results indicate that ALKBH5-mediated demethylation m6A at PTEN mRNA is involved in cadmium-induced cell transformation. Our study provides a new perspective for the involvement of m6A modification in cadmium carcinogenesis.
研究领域:
毒理学
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分子生物学
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癌症研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2020
·
IF 9.038
Hui-Jun Fu,Zi-Jian Chen,Hong Wang,Lin Luo,Yu Wang,Ri-Ming Huang,Zhen-Lin Xu,Bruce Hammock
Ethyl carbamate is a group of 2A carcinogen ubiquitously existed in fermented foods. The monitoring of its residues was important for evaluating the potential risk to human beings. Immunoassays with good accuracy and simplicity are great analytical tools for small molecule contaminants. However, it is typically confined in a competitive mode for small molecules with drawback of the sensitivity curbing. In this work, three different phages displayed peptides with capability of identifying the xanthyl ethyl carbamate immunocomplex were isolated from phage library. The binding mechanism of peptides and immunocomplex was studied by computer-assisted simulation. Results indicated that the xanthydrol group of xanthyl ethyl carbamate and the Asn-32 and Asn-92 residues of the antibody light chain were mainly responsible for binding. Simultaneously, a sensitive non-competitive immunoassay for detecting ethyl carbamate in wine samples was developed. The established method exhibited a limit of detection of 5.4 ng/mL and a linear range from 8.7 ng/mL to 32 ng/mL for wine samples. In comparison with the conventional competitive immunoassay, the sensitivity of the proposed non-competitive immunoassay was improved by 17-fold. The results of the immunoassay were validated by a standard ultra-performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry , which illustrated good reliability of the proposed assay.
研究领域:
食品安全
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分析化学
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生物信息学
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DOI
PubMed
高影响因子
FOOD CONTROL
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2021
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IF 5.548
Qian-Da Xu,Zhi-Qiang Zhou,Zan Jing,Qiang He,Qun Sun,Wei-Cai Zeng
Effect of pine needle extract from Cedrus deodara (PNE) on the quality and safety of Chinese traditional smoked bacon was observed, and its action mechanism was further investigated. With the treatment of PNE, the quality properties of smoked bacon, including moisture content, pH and titratable acidity values, color, texture and volatile compositions, were all ameliorated. Meanwhile, the peroxide value (POV), thiobarbituric acid value (TBARS) and nitrite content of smoked bacon with PNE decreased. In addition, the content of N -nitrosodimethylamine in smoked bacon gradually decreased with the increased concentration of PNE. Moreover, the heavy polycyclic aromatic hydrocarbons (PAHs) were not detected in smoked bacon with PNE, while the types and contents of light PAHs in smoked bacon were both reduced by PNE. With the measurement of fluorescence spectroscopy and circular dichroism spectroscopy , the secondary and tertiary structures of myofibrillar protein in smoked bacon were both changed though the treatment of PNE. Furthermore, according to the analysis of molecular docking, hydrogen bond was the main molecular force between PNE and meat proteins in smoked bacon. All present results suggested that PNE had the potential value to improve the quality and safety of smoked bacon in food industry.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
Environmental Science-Nano
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2024
·Vol. 11
·期 5
·1817-1846
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IF 5.8
Yiqun Mo,Yue Zhang,Qunwei Zhang
With the exponential growth of the nanotechnology field, the global nanotechnology market is on an upward track with fast-growing jobs. Nickel (Ni)-containing nanoparticles (NPs), an important class of transition metal nanoparticles, have been extensively used in industrial and biomedical fields due to their unique nanostructural, physical, and chemical properties. Millions of people have been/are going to be exposed to Ni-containing NPs in occupational and non-occupational settings. Therefore, there are increasing concerns over the hazardous effects of Ni-containing NPs on health and the environment. The respiratory tract is a major portal of entry for Ni-containing NPs; thus, the adverse effects of Ni-containing NPs on the respiratory system, especially the lungs, have been a focus of scientific study. This review summarized previous studies, published before December 1, 2023, on the cytotoxic, genotoxic, and carcinogenic effects of Ni-containing NPs on humans, lung cells in vitro, and rodent lungs in vivo, and the potential underlying mechanisms were also included. In addition, whether these adverse effects were induced by NPs themselves or Ni ions released from the NPs was also discussed. The extra-pulmonary effects of Ni-containing NPs were briefly mentioned. This review will provide us with a comprehensive view of the pulmonary effects of Ni-containing NPs and their underlying mechanisms, which will shed light on our future studies, including the urgency and necessity to produce engineered Ni-containing NPs with controlled and reduced toxicity, and also provide a scientific basis for developing nanoparticle exposure limits and policies.
研究领域:
毒理学
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环境健康
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纳米医学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2021
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IF 6.558
Kai Nan,Man He,Beibei Chen,Bin Hu
For a deep understanding of arsenic's mutagenicity , carcinogenicity and teratogenicity , the elucidation of arsenic binding proteins in organisms is a necessary prerequisite. Herein, a biotinylated phenylarsenite (Bio-PAO(III)) probe was synthesized for in situ binding to arsenic binding proteins in HepG2 cells. The Bio-PAO(III)-arsenic binding proteins complexes were captured by the prepared streptavidin-magnetic beads (SA-MBs) by specific interaction of biotin-SA. After magnetic separation, the arsenic binding proteins in the eluent was separated by sodium dodecyl sulfate-polyacrylamide - gel electrophoresis , and the in-gel tryptic digested protein bands were subjected to capillary high performance liquid chromatography coupled with electrospray ionization mass spectrometry analysis. 32 kinds of arsenic binding proteins were identified in HepG2 cells, which could be divided into three groups, structure proteins, enzymes related with tricarboxylic acid cycle and fatty synthesis and transcriptional regulator. Poly [ADP-ribose] polymerase 1 and general transcription factor IIH subunit 1 were identified to bind with arsenicals, which may affect the process of nucleotide excision repair in HepG2 cells.
研究领域:
毒理学
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蛋白质组学
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分子生物学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2024
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IF 8.2
Zhourui Xu,Jianjian Wei,Aroob Abid,Zirui Liu,Yasen Wu,Jia Gu,Dehua Ma,Min Zheng
Halobenzoquinones (HBQs) are a class of disinfection byproducts with high cytotoxicity and potential carcinogenicity , which have been widely detected in chlorination of drinking water and swimming pool water. However, to date, the formation of HBQs upon ozonation and the HBQ precursors have been overlooked. This study investigated the formation of chlorinated and dechlorinated HBQs from six dichlorophenol (DCP) isomers. The monomeric and dimeric HBQs were identified in all the ozonation effluents, exhibiting 1–100 times higher toxicity levels than their precursors. The sum of detected HBQs intensity had a satisfactory linear relation with the maximum toxic unit ( R 2 = 0.9657), indicating the primary toxicity contribution to the increased overall toxicity of effluents. Based on density functional theory calculations, when ozone attacks the para carbon to the hydroxyl group of 2,3-DCP, the probability of producing chlorinated HBQs is 80.41 %, indicating that the para carbon attack mainly resulted in the formation of monomeric HBQs. 2,3-dichlorophenoxy radicals were successfully detected in ozonated 2,3-DCP effluent through electron paramagnetic resonance and further validated using theoretical calculation, revealing the formation pathway of dimeric HBQs. The results indicate that chlorinated phenols, regardless of the positions of chlorine substitution, can potentially serve as precursors for both chlorinated and dechlorinated HBQs formation during ozonation.
研究领域:
环境科学
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毒理学
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水处理工程
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产品关联
DOI
PubMed
高影响因子
Environmental Technology & Innovation
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2024
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IF 6.7
Kunfeng Zhang,Sheng Chang,Qi Zhang,Yunsong Bai,Enrui Wang,Yueting Fan,Xiang Tu,Qing Fu,Liangliang Wei,Yanling Yu
This study investigates the composition and distribution of 16 priority polycyclic aromatic hydrocarbons (PAHs) in 70 water samples collected from centralized drinking water sources (CDWSs) across 30 provinces (cities) in mainland China. Additionally, water quality and socio-economic data were collected to determine the influencing factors. The study discovered that the total concentrations of 16 priority PAHs (Σ 16 PAHs) varied from 0.17 to 176.71 ng/L (mean: 56.49 ± 38.58 ng/L). Lakes and reservoirs had significantly higher concentrations of PAHs than groundwater. The primary pollutants were low-molecular-weight (LMW) PAHs, such as phenanthrene, naphthalene, and fluorene. There was a strong correlation between LMW PAHs and Σ 16 PAHs ( p < 0.05). The mean Σ 16 PAHs in the seven geo-environmental zones were ranked as follows, under the comprehensive influence of emission density, natural, and social environmental factors: Northwest China (78.42 ± 66.96 ng/L) > Northeast China (73.70 ± 33.23 ng/L) > East China (64.35 ± 45.66 ng/L) > North China (50.95 ± 31.87 ng/L) > Southwest China (44.05 ± 32.56 ng/L) > Central China (41.68 ± 19.44 ng/L) > Southern China (40.99 ± 16.54 ng/L). The source modeling analysis revealed that the majority of PAHs came from chemical production and metal smelting (42.36%), as well as from the combustion of coal, wood, and biomass (34.17%), and other mixed sources. In addition, the study found a strong correlation between water quality and socio-economic factors with concentrations of PAHs, indicating that these factors may contribute to the re-enrichment, transport, and transformation of PAHs in overlying water to varying degrees. The mean incremental lifetime cancer risk (ILCR) was lower than the risk threshold. These findings provide scientific references for managing hazardous substances in the aquatic environment.
研究领域:
环境科学
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公共卫生
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毒理学
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产品关联
DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2021
·Vol. 105
·期 13
·5607-5616
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IF 4.813
He Ying,Ding Haotian,Xia Xingya,Qi Wenyi,Wang Huaisong,Liu Wenyuan,Zheng Feng
Abstract Nitrosamine compounds, represented by N -nitrosodimethylamine, are regarded as potentially genotoxic impurities (PGIs) due to their hazard warning structure, which has attracted great attention of pharmaceutical companies and regulatory authorities. At present, great research gaps exist in genotoxicity assessment and carcinogenicity comparison of nitrosamine compounds. In this work, a collection of GFP-fused yeast cells representing DNA damage repair pathways were used to evaluate the genotoxicity of eight nitrosamine compounds (10 −6 –10 5 μg/mL). The high-resolution expression profiles of GFP-fused protein revealed the details of the DNA damage repair of nitrosamines. Studies have shown that nitrosamine compounds can cause extensive DNA damage and activate multiple repair pathways. The evaluation criteria based on the total expression level of protein show a good correlation with the mammalian carcinogenicity data TD 50 , and the yeast cell collection can be used as a potential reliable criterion for evaluating the carcinogenicity of compounds. The assay based on DNA damage pathway integration has high sensitivity and can be used as a supplementary method for the evaluation of trace PGIs in actual production. Key points • The genotoxicity mechanism of nitrosamines was systematically studied . • The influence of compound structure on the efficacy of genotoxicity was explored . • GFP-fused yeast cells have the potential to evaluate impurities in production . Graphical abstract
研究领域:
毒理学
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分子生物学
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药学
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DOI
PubMed
高影响因子
MARINE POLLUTION BULLETIN
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2023
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IF 5.8
Xiaoqin Lin,Luanxun Lin,Zelong Liao,Pengfei Wu,Chunxue Yang
This study investigated the levels and composition of 16 priority polycyclic aromatic hydrocarbons (PAHs) in marine organisms from South China Sea and assessed their potential health risks. The results revealed that the pollution levels of total PAHs ranged from 3.56 to 392.21 ng/g dw. Notably, 4-ring PAHs constituted the predominant fraction (58.02 %) of the total PAHs, with pyrene being the most abundant congener across all species. Intriguingly, our findings suggested that consuming these organisms might pose a low non-cancer hazard. Nonetheless, benzo[ a ]pyrene was detected in most species, with levels ranging from non-detectable to 11.24 ng/g dw. The individual lifetime cancer risk levels associated with seafood consumption in studied regions ranged from 1.10 × 10 −5 to 1.52 × 10 −5 , highlighting a potential cancer risk that warrants special attention. These findings emphasize the need to prioritize carcinogenic compounds over total PAHs and underscore the importance of continuous monitoring of PAH pollution in seafood.
研究领域:
环境科学
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毒理学
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公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）