Bioactive Materials
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2021
·
IF 14.593
Danfeng Liu,Xianbin Ma,Yaoting Ji,Rourong Chen,Shuhui Zhou,Hantao Yao,Zichen Zhang,Mengjie Ye,Zhigang Xu,Minquan Du
Early childhood caries (ECC) is a public healthcare concern that greatly reduces the quality of life of young children. As a leading factor of ECC, cariogenic biofilms are composed of acidogenic/aciduric pathogens and extracellular polysaccharides (EPSs), creating an acidic and protected microenvironment. Antimicrobial photodynamic therapy (aPDT) is a noninvasive, painless, and efficient therapeutic approach that is suitable for treating ECC. However, due to the hyperfine structure of cariogenic biofilms, most photosensitizers (PSs) could not access and penetrate deeply in biofilms, which dramatically hamper their efficiency in the clinic. Herein, bioresponsive nanoparticle loaded with chlorin e6 (MPP-Ce6) is developed, which largely increases the penetration depth (by over 75%) and retention (by over 100%) of PS in the biofilm compared with free Ce6. Furthermore, MPP-Ce6-mediated aPDT not only kills the bacteria in preformed biofilms but also inhibits multispecies biofilm formation. A rampant caries model is established to mimic ECC in vivo , where the population of cariogenic bacteria is decreased to 10% after MPP-Ce6-mediated aPDT. Importantly, the number and severity of carious lesions are efficiently reduced via Keyes’ scoring and micro-CT analysis. This simple but effective strategy can serve as a promising approach for daily oral hygiene in preventing ECC.
研究领域:
口腔医学
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纳米医学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Bioactive Materials
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2025
·
IF 20.3
Jiayi Shi,Xuekai Qi,Ying Ran,Qiang Zhou,Yiqin Ding,Lujian Li,Youyun Zeng,Dongchao Qiu,Zhibin Cai,Xiaojun Cai,Yihuai Pan
Dental caries is primarily caused by cariogenic bacteria metabolizing carbohydrates to produce acidic substances that erode the dental hard tissues. Traditional remineralization treatments often have limited efficacy due to their lack of antibacterial activity. According to the Interrupting Dental Caries (IDC) theory, ideal caries-preventive materials should possess both antibacterial and remineralizing properties. Furthermore, effective adhesion to dental surfaces is crucial. Inspired by the wet adhesion properties of the salivary acquired pellicle, we developed a multifunctional gargle named Ce6@PDN-SAP (CP-SAP). This formulation employed peptide dendrimer nanogels (PDN) as a carrier for the photosensitizer Ce6, further functionalized with saliva-acquired peptide (SAP) to confer wet adhesion properties. CP-SAP rapidly adhered to the dental surface and remained effective for extended periods. Upon laser irradiation, Ce6 generated reactive oxygen species (ROS), disrupting bacterial outer membrane integrity, causing protein leakage, and reducing ATP levels, thereby achieving potent antibacterial effects. Following this, PDN and SAP acted as nucleation templates to promote in situ remineralization of demineralized dental hard tissues. In vivo studies using rat models demonstrated that CP-SAP provided significantly superior caries-preventive effects compared to chlorhexidine gargle. In conclusion, CP-SAP, as an innovative approach grounded in the IDC theory, holds great promise for the prevention and treatment of dental caries.
研究领域:
牙科学
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材料科学
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生物医学工程
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 8
·1-10
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IF 5.3
Qian Lelin,Yu Zhangli,Zhang Yuan,Huang Jingyi,Ye Daixin,Niu Lili,Zhang Juan
Dental caries, one of the most common oral diseases, is mainly induced by multiple cariogenic bacteria in the oral microenvironment, so it is important to construct a method that can identify oral multiply cariogenic bacteria. Herein, a machine learning assisted colorimetric sensing array has been constructed by using polyethylene glycolized graphyne nanozymes, which have been firstly prepared through click chemistry to enhance the peroxidase-like catalytic activity and stability. Taking advantage of different interactions between cariogenic bacteria and polyethylene glycolized graphyne nanozymes, the sensing array can well identify ten oral cariogenic bacteria and their binary and ternary complexes by linear discriminant analysis algorithm. In view of its high accuracy to analyze the spiked artificial saliva samples, the developed colorimetric sensing array owns a great potential for the diagnosis of dental caries. Graphical
研究领域:
纳米技术
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生物传感器
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牙科医学
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微生物学
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材料科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）