SENSORS AND ACTUATORS B-CHEMICAL
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2023
·
IF 8.4
Jiaojiao Yang,Lijun Feng,Jing Liu,Sheng Li,Na Li,Xinfeng Zhang
Mercury (Hg) is an important environmental pollutant because of its high toxicity. With the rapid industrialization, on-site monitoring Hg 2+ contamination becomes increasingly important. In this work, a facile colorimetric sensing system based on DNA-mediated charge neutralization of AuNPs as well as smartphone data acquisition was developed for on-site monitoring of Hg 2+ . The designed both-end block dsDNA system (probe DNA and block DNA) can interact with the replacement DNA and formed T-Hg 2+ -T mismatched dsDNA in the presence of Hg 2+ , thus releasing the long block DNA as well as preventing the ThT-induced neutralization of AuNPs. The color change for the AuNPs neutralization can be pictured and analyzed by a portable smartphone. This on-site Hg 2+ monitoring system was quite simple and sensitive with a low limit of detection (2.8 nM), lower than the maximum permitted amount of Hg 2+ in drinking water regulated by EPA. The accuracy of the proposed method was verified through analysis of two certified water samples, four real water samples and seven cosmetic samples, and the results agreed well with the certified values or those by atomic fluorescence spectrometry . Overall, this facile and simple sensing system is an appealing tool for on-site monitoring of Hg 2+ in water and cosmetic samples.
研究领域:
环境科学
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分析化学
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生物传感
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产品关联
DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 29
·16095-16102
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IF 2.936
Guojuan Ren,Liying Yu,Baoya Zhu,Mingyu Tang,Fang Chai,Chungang Wang,Zhongmin Su
In this study, infrequent orange carbon nanodots (CNDs) were applied as a dual-readout probe for the effective colorimetric and fluorescent detection of 2,4,6-trinitrophenol (TNP). The orange fluorescence could be rapidly and selectively quenched by TNP, and the colorimetric response from the original pink color to blue could also be captured immediately by the naked eye. A limit of detection of 0.127 μM for TNP was estimated by the fluorescent method and 5 × 10−5 M by visualized detection. Interestingly, the fluorescence of the CNDs with TNP gradually transitioned from orange to green upon irradiation by a UV lamp, and the colorimetric response transitioned from pink to blue to colorless, which ensured effective multi-response detection of TNP. In addition, the CNDs exhibited bright fluorescence, excellent biocompatibility and low toxicity, making them high-quality fluorescent probes for cellular imaging.
研究领域:
分析化学
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材料科学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Genetics
·
2025
·
IF 2.8
Xin Wang,Longxin Xu,Di Zhou,Yanli Lv,Junda Wu,Yuanfeng Zhao,Mengmeng Ni,Wenzhang Zhou,Kaikai Zhang,Hua Wang,Jipan Zhang
Objective: Coat color is a complex trait and plays an important role in breed identification. However, information regarding genes associated with coat color in cattle is limited, especially at the skin transcriptome level.Methods: We investigated the differential expressed genes (DEGs) and genomic selection signal underlying the coat color variation between black and brown cattle breeds. A total of 19 cattle (Brangus, Angus, Simmental, and Guanling) were performed skin transcriptome analysis and 262 cattle (Angus and Simmental) were performed whole genome analysis.Results: Angus cattle (black coat) had a significantly higher melanin content in both their hair and skin compared to that of Simmental and Guanling cattle (brown coat). Transcriptomic analysis identified 14,118 expressed genes, with principal component analysis and hierarchical clustering revealing clear differences between black and brown cattle. DEGs analysis across four pairwise breed comparisons highlighted 343 downregulated and 54 upregulated genes common to all comparisons, with the ASIP gene (agouti signaling protein) emerging as a key gene linked to melanogenesis. The ASIP expression was several dozen-fold higher in brown cattle than in black cattle, suggesting a crucial role in coat color determination. Path-way enrichment and gene set enrichment analysis (GSEA) identified the “Melanogenesis” pathway as significantly enriched and central to coat color variation. Genes such as FZD10, WNT6, and ASIP showed differential expression patterns that correlated with coat color. Genomic analysis revealed strong selection signals in the ASIP gene region, with several SNPs exhibiting high linkage disequilibrium. Notably, the mutation type was predominant in Simmental cattle, while the reference allele was more common in Angus cattle.Conclusion: Based on the skin transcriptomic and genomic analyses, we found that ASIP was significantly differential expressed between black and brown cattle breeds and under strong positive selection. These findings provide valuable insights into the genetic basis of coat color variation in cattle and highlight the ASIP gene as a critical determinant of this trait.
研究领域:
遗传学
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分子生物学
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DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 73
·59056-59061
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IF 3.84
Lun Song,Haiyu Tian,Xiaoliang Pei,Ziyou Zhang,Weibing Zhang,Junhong Qian
Two water-soluble colorimetric and turn-on fluorescent probes STP1–2 were rationally designed and synthesized for selective recognition of GSH. Both probes are colorless 4-thioether-1,8-naphthalimide derivatives, which are almost non-fluorescent due to the photoinduced electron transfer from the fluorophore to the 4-nitrobenzene. Upon addition of GSH to STP1/STP2–CTAB solution, obvious spectral responses were observed: the absorption peak shifted from 345 nm to 390 nm companied with ∼90-fold fluorescence enhancement at 487 nm. A good linear relationship between the fluorescence intensity and GSH concentration was obtained, and the detection limit of GSH was estimated to be 8.40 × 10−8 mol L−1. The experimental results imply that both probes could be applied in fluorescent imaging of GSH within living cells and in the detection of mercapto-containing proteins as well.
研究领域:
生物化学
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分析化学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Xiangyu Jiang,Fenghua Geng,Yongxiang Wang,Jinhua Liu,Peng Qu,Maotian Xu
Quantitative fluorescence turn-on and colorimetric detection of glutathione (GSH) with rapid speed, low cost have attained much attention. Herein, we developed a sensitive fluorescence turn-on and colorimetric sensor for GSH based on the inner-filter effect (IFE), which is the first time to select oxTMB and TMPyP as the IFE absorber and fluorophore pair, respectively. The absorption band of oxTMB matches well with the emission band of TMPyP in the IFE-based fluorescent assay. In the absence of GSH, the absorption peak of oxTMB at 652 nm significantly overlaps with the emission of TMPyP, resulting in the efficient IFE and inhibition of the fluorescence of TMPyP. In the presence of GSH, the absorption intensity at 652 nm decreases, generating the recovery of the fluorescence of TMPyP. Therefore, this approach is demonstrated to be a novel candidate for detection of GSH, with high sensitivity and selectivity. The linear dynamic range for the concentrations of GSH is between 0.1 μM to 20 μM along with a limit of detection (LOD) of about 30 nM (calculated LOD as 3 σ /slope). Finally, this novel sensor was successfully applied for GSH detection in fetal calf serum , and satisfactory recovery was achieved.
研究领域:
分析化学
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生物传感
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医学检测
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2020
·
IF 4.006
Meizhen Xie,Xiyu Dong,Yan Yu,Leqi Cui
Lipid oxidation is a major food safety issue for edible oils . The objective of this study was to develop a simple method for detecting lipid oxidation through the color change of a composite film. Schiff's reagent which can form color compound with lipid oxidation products—aldehydes was incorporated into polyvinyl alcohol (PVA) to form the composite film. The effects of PVA molecular weight (Mw), Schiff's reagent/PVA ratio and glycerol content on the response of the composite film to aldehyde were investigated. Sunflower seed oil with various extent of oxidation was used to evaluate the effectiveness of the composite film for lipid oxidation detection. The results showed that the composite film formed with lower PVA Mw turned from colorless to purple upon aldehyde exposure. Higher Schiff's reagent/PVA ratio and glycerol content led to shorter response time and larger color change after aldehyde exposure. The color change of the composite film was linearly (R 2 = 0.96) related to aldehyde concentration. The oxidation status of four sunflower seed oil samples out of five were correctly identified by the composite film. Although the accuracy of this method was 80% in this study, it still shows great potential for lipid oxidation detection application.
研究领域:
食品科学
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化学
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产品关联
DOI
高影响因子
Smart Materials and Structures
·
2020
·Vol. 29
·期 4
·04LT02
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IF 3.613
Liangliang Cao,Liang Fang,Xue Li,Shunping Chen,Chunhua Lu,Zhongzi Xu
Chameleons are attractive animals, which can change their colors because of unique layered skin structures. Inspired by this natural design, we explored a layer-by-layer assembly technique to create thermochromic films that can attain reversible and multiple-colors change. Briefly, inorganic pigments and two kinds of thermochromic microcapsules having different responsive temperatures were assembled sequentially onto a piece of filter paper using extraction filtration before epoxy resin was used to seal the whole structure. Different functions including colorful patterns and luminescent letters can be integrated into the system using this versatile approach. More importantly, arrays of thermochromic films were used with a detector and a heating-control circuit to trigger complicated color change by varying detected colors. We believe that this cost-efficient and convenient approach can be used in multiple applications such as camouflage, anti-counterfeiting, smart constructions and buildings, etc.
研究领域:
材料科学
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仿生学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2015
·
IF 4.097
Xiao-Hong Cao,Hao-Yan Zhang,Ru-Chao Ma,Qian Yang,Zhi-Bin Zhang,Yun-Hai Liu
A simple and selective colorimetric sensor based on o-phosphorylethanolamine-functionalized gold nanoparticles (OPA–Au NPs) as an optical probe has been developed for the detection of UO 2 2+ . OPA–Au NPs rapidly aggregate in the presence of UO 2 2+ , thereby causing a visual color change from red to blue, which is easily detectable by eye. The proposed sensing system possesses a selective response for UO 2 2+ over other heavy metal ions. The current method shows application potential for UO 2 2+ detection in real water samples.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
ANALYTICAL SCIENCES
·
2018
·Vol. 34
·期 11
·1297-1302
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IF 1.355
Wang Hao,Wu Xiaoming,Tao Feiyan,Yang Shaoxiang,Tian Hongyu,Liu Yongguo,Sun Baoguo
A new highly selective and visible colorimetric fluorescent probe (probe 1) was developed to detect hydrazine (N2H4) concentration in real water samples. As different concentrations of N 2 H 4 were added, the color of the probe solution was graded gradually from colorless to pink, which could be observed by the naked eye under UV light at 365 nm. Our research indicates that probe 1 offers a certain practical significance for use as a visible detection agent to detect N2H4 efficiently by distinct color response. Furthermore, our work showed that probe 1 can be successfully applied to detect N2H4 concentrations in real water samples.
研究领域:
分析化学
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环境科学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry C
·
2016
·Vol. 4
·期 41
·9651-9655
·
IF 5.066
Wei Xu,Feiming Li,Zhixiong Cai,Yiru Wang,Feng Luo,Xi Chen
An organic–inorganic hybrid fluorescent perovskite, CH3NH3PbBr3, was synthesized by a grinding method, and applied for humidity sensing. Its strong fluorescence at 530 nm could be linearly quenched by a humidity increase from 7 to 98%. Coupled with a red-emitting reference dye, 5,10,15,20-tetrakis(pentafluorophenyl)porphyrin, a colorimetric humidity sensor was produced using the perovskite. The sensor appeared to be sensitive to increased humidity by displaying a color change from green to red, or vice versa, with good reversibility. The proposed approach should provide a new strategy in the design of sensitive and applicable humidity sensors.
研究领域:
材料科学
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光学
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产品关联
DOI
高影响因子
ACS Omega
·
2023
·
IF 4.1
Lina Wen,Xiaoyu Du,Tianci Liu,Wen Meng,Tao Li,Mengjie Li,Man Zhang
Clusterin has the potential to become the biomarker of multiple diseases, but its clinical quantitative detection methods are limited, which restricts its research progress as a biomarker. A rapid and visible colorimetric sensor for clusterin detection based on sodium chloride-induced aggregation characteristic of gold nanoparticles (AuNPs) was successfully constructed. Unlike the existing methods based on antigen–antibody recognition reactions, the aptamer of clusterin was used as the sensing recognition element. The aptamer could protect AuNPs from aggregation caused by sodium chloride, but clusterin bound with aptamer detached it from AuNPs, thereby inducing aggregation again. Simultaneously, the color change from red in the dispersed state to purple gray in the aggregated state made it possible to preliminarily judge the concentration of clusterin by observation. This biosensor showed a linear range of 0.02–2 ng/mL and good sensitivity with a detection limit of 5.37 pg/mL. The test results of clusterin in spiked human urine confirmed that the recovery rate was satisfactory. The proposed strategy is helpful for the development of label-free point-of-care testing equipment for clinical testing of clusterin, which is cost-effective and feasible.
研究领域:
生物传感
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临床诊断
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
·
IF 5.8
Yu-Xi Bi,Dan Zhao,Shao-Jie Dai,Rui-Juan Zhang,Qing-Xia Yao
Ion doping is one of the most effective strategies to induce interesting luminescence properties of materials. In this work, the Bi 3+ and Eu 3+ are introduced into a ‘114’ host material YBaZn 3 GaO 7 (YBZG) to generate photoluminescence (PL), long-persistent luminescence (LPL) and colour change properties. The co-doped YBZG:0.003Bi 3+ / y Eu 3+ can generate multi-color emissions from green, through yellow to orange with the increasing of Eu 3+ concentration. There are F-like color centers around conduction band (CB) of YBZG host with shallow or deep energy levels. The electrons trapped by deep levels response to the PC properties, whereas these for shallow levels response to the LPL properties. The PC process from white to apricot is reversible. By alternating thermal treatment and irradiation, YBZG:0.003Bi 3+ was proved to have a good fatigue resistance of 3.1 %. For YBZG:0.003Bi 3+ /0.05Eu 3+ , it shows bright orange LPL emission when the irradiation is removed after 24 min. The achievement of this paper provides a design idea that if the host structure of a phosphor is of flexible and easy to occur defect, not only PL property, but also PC and LPL properties are probably able to realize.
研究领域:
材料科学
·
光学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）