Frontiers in Energy Research
·
2022
·
IF 3.858
Kiani Hossein,Aznar Ramón,Poojary Mahesha M.,Tiwari Brijesh K.,Halim Ronald
Microalgae are potential sources for the sustainable production of valuable chemicals including polyphenols, pigments, and ω-3 PUFAs. However, successful exploitation of these high value compounds in the food, healthcare and pharmaceutical sectors depends greatly on their effective separation, identification, and analysis after recovery from the biomass. The findings of this review paper illustrated that chromatographic methods coupled to different types of detectors have been used as a crucial part of research on microalgal polyphenols, Omega-3 Polyunsaturated Fatty Acids (ω-3 PUFAs), and pigments production through identification, measurement, sample preparation, and purification practices. Therefore, it is important to provide a comprehensive review regarding the current research in the field. The basic operating principles, parametric optimisation and detection units of common (liquid chromatography and gas chromatography) and novel chromatographic techniques (counter current chromatography, expanded bed adsorption chromatography and supercritical fluid chromatography) used to separate, identify, and quantify polyphenols, PUFAs and pigments from microalgae matrices are comprehensively reviewed.
研究领域:
生物化学
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食品科学
·
药学
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DOI
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Fang Zhang,Jie Qu,Kiran Thakur,Jian-Guo Zhang,Andrei Mocan,Zhao-Jun Wei
The present study demonstrated the hydrolysis of peony seed protein isolate (PSPI) by using alcalase and resulted in the generation of an anti-oxidative peptide. In brief, a model was used to illustrate the enzymolysis of PSPI with the determination of kinetic factors as per investigation information. The model proved suitable to explain the PSPI hydrolysis by alcalase. A novel anti-oxidative peptide was obtained successfully by ultrafiltration and a series of chromatography techniques. Subsequently, a purified fragment was identified with the amino acid sequence of SMRKPPG followed by its synthesis and evaluation of its anti-oxidative activities. After hydrolysis, the peony seed protein hydrolysate (PSPH) with the degree of hydrolysis of 18% displayed the most significant antioxidant action which was further used to isolate the anti-oxidative peptide.
研究领域:
植物化学
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食品科学
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生物化学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Guoning Chen,Shuxian Zhang,Xiaofei Wang,Xiaoxuan Fan,Gidion Wilson,Yuping Sa,Xueqin Ma
The discovery of enzyme inhibitors from natural products is a crucial aspect in the development of therapeutic drugs. However, the complexity of natural products presents a challenge in developing simple and efficient methods for inhibitor screening. Herein, we have developed an integrated analytical model for screening xanthine oxidase (XOD) inhibitors that combines simplicity, accuracy, and efficiency. This model utilizes a colorimetric sensor and affinity chromatography technology with immobilized XOD . The colorimetric sensor procedure can quickly identify whether there are active components in complex samples. Subsequently, the active components in the samples identified by the colorimetric sensor procedure were further captured, separated, and identified through affinity chromatography . The integrated analytical model can significantly enhance the efficiency and accuracy of inhibitor screening. The proposed method was applied to screen for an activity inhibitor of XOD in five natural medicines. As a result, a potential active ingredient for XOD , polydatin , was successfully identified from Polygoni Cuspidati Rhizoma et Radix. This work is anticipated to offer new insights for the screening of enzyme inhibitors from natural medicines.
研究领域:
药物化学
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生物分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2022
·
IF 3.318
Yu-Ming Fang,Qi-Lei Zhang,Dong-Qiang Lin,Shan-Jing Yao
Short peptide biomimetic chromatography technology as a developing protein separation technology has huge potential for antibody purification. In this study, four tetrapeptide ligands (Ac-FYKH, Ac-YEHF, Ac-YFLH and Ac-FYHI) with high potential binding ability to antibody were selected for the optimal ligand to antibody purification. The results showed that Ac-YEHF-4FF resin had higher binding capacity and selectivity for hIgG among the four resins. And at pH 7.0 and 0.3 ml/min, the highest Q 10%-hIgG of Ac-YEHF-4FF resin was 26.2 mg/ml resin while its Q 10%-BSA was just 2.2 mg/ml resin. Further, Ac-YEHF-4FF resin was used to purify protein mixtures. By binding at pH 7.0 and being eluted at pH 5.0 and pH 4.0, Ac-YEHF-4FF resin was well used to separate hIgG from BSA containing feedstock, HSA containing feedstock and human serum with the purity and yield both more than 95 %. And the screened resin could also separate mAb from CHO cell culture supernatant with purity 94.3 % and yield 97.5 %. The adsorption and separation results of Ac-YEHF-4FF resin indicated that the goal of getting the efficacy of critical residues from protein A to biomimetic its structure and function could be achieved, which had great significance to the establishment and improvement of tetrapeptide biomimetic chromatography, and also provided a new method for the field of antibody separation and purification.
研究领域:
生物化学
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蛋白质工程
·
分离技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Xiao-Dong Cheng,Zheng Zhang,Yun-Ping Li
Green chromatography techniques using low-toxic mobile phase are getting increasingly attention in recent years. The core is developing stationary phases that possess adequate retention and separation under the mobile phase of high content water. Using thiol-ene click chemistry, an undecylenic acid-bonded silica stationary phase (UAS) was prepared in a facile manner. Elemental analysis (EA), solid-state 13 C NMR spectroscopy and Fourier transform infrared spectrometry (FT-IR) confirmed the successful preparation of UAS . The synthesized UAS was employed for per aqueous liquid chromatography (PALC), which uses little organic solvent during separation. Due to the hydrophilic carboxy, thioether group and hydrophobic alkyl chains of the UAS, various categories of compounds (including nucleobases , nucleosides , organic acids and basic compounds) with different properties can achieve enhanced separation under the mobile phase of high content water compared with commercial C18 and silica stationary phases. Overall, our present UAS stationary phase shows excellent separation ability toward highly polar compounds and meets the requirements of green chromatography.
研究领域:
分析化学
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色谱技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Yangzhou Xie,Sitao Wang,Yu Tian,Kexi Ye,Zhigang Xu,Han Zheng,Wei Jiang,Fengmei Zhang,Xiaoxi Si
Excessive D-lactic acid in fermented foods can result in unpleasant flavors and is difficult for the human body to metabolize. This study is the first to combine tandem column technology (C18 and Poroshell chiral-T) with a machine learning strategy. The method achieved a resolution of 2.86, with a concentration range of 50.00 μg·L −1 -50.00 mg·L −1 and a limit of detection of 16.67 μg·L −1 . We aimed to classify different lactic acid products and tobacco, and the research showed that L-lactic acid was predominant in all food analyses , while D-lactic acid was only found in lactic acid products In addition, the machine learning algorithms were optimized, and the random forest model effectively distinguished samples with similar lactic acid content. Based on this collaborative model of tandem column and machine learning, lactic acid chiral biomarkers gained the ability to classify food samples. The accuracy of model reached 80 %. This strategy contributes to the chiral analysis of lactic acid in complex food matrices. It also demonstrates the practical value of classification in food research.
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2025
·
IF 2.8
Qingqing Yao,Jiatai Yin,Xiuli Ji,Xue Li,Yifan Gao,Dan Lu,Ying Chen,Qian Li,Dalong Zhi
Despite considerable efforts invested in clinical trials aimed at treating obesity and enhancing the metabolic profiles of Nelumbinis Folium , the precise phytochemicals involved and their mechanisms of action remain unclear due to the absence of an efficient screening technique. Herein, Nelumbinis Folium serves as the focal point to elucidate the bioactive compounds that specifically bind to peroxisome proliferator-activated receptor γ using immobilized receptor chromatography. Following identification through liquid chromatography-mass spectrometry, the compounds were further evaluated using chromatographic techniques and molecular dynamics simulations. The results unveiled catechin and hypericin as the receptor-binding compounds present in Nelumbinis Folium , with hypericin exhibiting a stronger affinity and a faster dissociation rate constant compared to catechin. Molecular dynamics studies highlighted the crucial role of cysteine located at position of 285 in the receptor ligand binding domain during the initial ligand capture phase. Subsequently, Van Der Waals forces and electrostatic interactions facilitated the binding process. The calculated standard binding free energies were − 61.75 ± 2.61 kcal/mol for hypericin and − 43.19 ± 0.63 kcal/mol for catechin. Collectively, these findings provide valuable insights into receptor-drug interactions and confirm the effectiveness of immobilized receptor chromatography in screening potential lead compounds from complex systems.
研究领域:
药理学
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植物化学
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计算生物学
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产品关联
DOI
PubMed
高影响因子
Energy Storage Materials
·
2024
·
IF 18.9
Yaoyao Liu,Feng Zhang,Zhaofen Wang,Lequan Deng,Lutan Dong,Ke-Peng Song,Shuhua Wang,Hong Liu,Hao Chen
Electrically isolated metallic Zn (Zn 0 ) and electrochemically side reaction (ECSR), causes irreversible anode capacity loss and limited cycle life in zinc metal battery. However, the quantitative distinguishment between inactive metallic Zn 0 and ECSR, their formation mechanism and correlation with anode cycling reversibility have never been disclosed, limiting further anode modification design. Here, we develop an acid-assisted continuous titration-collection-gas chromatography technique (AAC-TCGC) that accurately quantify the amount of inactive Zn 0 and ECSR in anode, and discover that inactive Zn 0 accounts for the majority of Zn loss, rather than the commonly-assumed electrochemically side reaction-derived ECSR-dominant Zn anode capacity loss. Detailed component of SEI (Solid Electrolyte Interface Layer) compound is also characterized, illustrating a solvent-derived zinc oxide/hydroxide-dominant SEI that facilitates vertical/inclined thin-plate Zn deposition, but salt-derived ZnF 2 -dominant SEI that favors horizontal platelet-shaped Zn(002) deposition. Simulation results reveal a new electric resistance-derived, bottom-preferred Zn stripping mechanism that leads to abundant inactive Zn 0 formation in vertical thin-plate Zn deposition structure, but few inactive Zn 0 in dumpy platelet-shaped Zn structure. Our study provides new theories and strategies on the distinguishment, formation mechanism and structure-performance relationship of inactive Zn, important for building more efficient Zn metal battery.
研究领域:
电化学
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材料科学
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电池技术
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shuangyu Shao,Xiali Si,Yingtao Zhang,Jun Li,Pengfei Tu,Qingying Zhang
Quality analysis of edible mushrooms based on polysaccharides is generally difficult due to their complicated structures and hard separation. Here, multiple fingerprint analysis of polysaccharides based on chromatographic and spectrometric techniques were developed, and then applied in comparative analysis of Auricularia heimuer (AH), Auricularia cornea (AC), Auricularia cornea ʻYu Muerʼ (ACY) and Tremella fuciformis (TF). Firstly, polysaccharides were obtained with the molecular weights between 1.783 × 10 6 and 6.774 × 10 6 Da. Then, complete hydrolysis by TFA and enzyme digestion by cellulase were employed and subsequently analyzed by HPLC-UV, GC–MS, HILIC-HPLC-ELSD and HILIC-HPLC-ESI − -HCD-MS/MS, and ATR-FT-IR were used to characterize the functional groups of intact polysaccharides. By chemometric analysis, differential markers of d -xyl, l -fuc, l -arb, d -glc, disaccharide and hexasaccharide were selected, and AC and ACY were proved to be same species from the viewpoint of polysaccharides firstly. Furthermore, the structures of oligomers with DPs of 2–8 and →4)- β - d -Glc p -(1→ unit with different contents were inferred by combinatory analysis of ESI − -MS/MS, glycosidic linkage, monosaccharide compositions and functional groups. In conclusion, the combinatory method of multiple fingerprint and pattern recognition is powerful not only for structural elucidation of polysaccharides, but also for quality analysis and species differentiation of edible mushrooms from the perspective of biological polysaccharides.
研究领域:
食品科学
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分析化学
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真菌学
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生物化学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Wenxin Wang,Huanxin Xu,Fanghong Ning,Junfen Wan
Ascomycin plays an important role in the treatment of various autoimmune diseases and skin diseases; its application prospects in the pharmaceutical market are promising. As an intermediate of pimecrolimus, separating and purifying high-purity ascomycin is a key step in producing high-quality medicines. Moreover, ascomycin and its key impurities have similar structures, which makes the separation and purification process very challenging. Therefore, this study developed a new separation and purification system that combines the synergistic effects of reversed-phase polymer resin and macroporous adsorption resin to provide a novel separation and preparation process. In the static adsorption stage, HZ-816 was chosen as the optimal resin, and used Lagergren's second-order kinetic and the Temkin isotherm model as the best adsorption models; the adsorption process was spontaneous, exothermic, and multi-layer adsorption. To achieve optimal purification, we used 0.5 mg/mL ascomycin, a 4 BV/h flow rate, and a sample volume of 110 BV. The eluent was 70 % ethanol, with a flow rate of 2 BV/h. The obtained purity was 82.07 %, and the recovery rate was 62.68 %. During the reversed-phase polymer chromatography stage, by optimizing parameters such as the elution flow rate and loading quantity, the purity was increased to 98.55 %, with a recovery rate of 85.87 % and an elution rate of 95.54 %. The yield was 53.82 %. Through the optimization of experimental procedures and material selection, this study has laid a solid performance foundation for subsequent cost-effective, highly efficient, and sustainable applications, providing a reference for the technological advancement of the ascomycin separation and purification industry.
研究领域:
药物化学
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分离科学
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色谱技术
·
生物过程工程
·
药物研发
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产品关联
DOI
高影响因子
CHROMATOGRAPHIA
·
2021
·Vol. 84
·期 4
·325-333
·
IF 2.044
Shuai Xiaomin,Cai Zhiqiang,Zhao Xinyu,Chen Yujie,Zhang Qian,Ma Ziwei,Hu Junjie,Sun Tao,Hu Shaoqiang
In this work, a novel calix[4]resorcinarene-based ionic liquid (C4RA-2IL) was synthesized, structurally characterized, and statically coated on capillary column as stationary phase for capillary gas chromatography (GC). The column efficiency of the C4RA-2IL column is 3345 plates m −1 , which are determined by n -dodecane at 120 °C. Based on its McReynolds constants, the C4RA-2IL column showed moderate polarity. Particularly, the C4RA-2IL column show high separation performance for a wide range of analytes and some difficult separation of meta/para-isomers. Moreover, it exhibited excellent selectivity for critical aromatic isomers of chloroaniline, bromaniline, iodoaniline, toluidine and xylidine isomers and shows advantageous separation capability over the commercial polysiloxane stationary phase. This work presents a promising future of calixarene-based ionic liquid as a new type of stationary phase in GC separations.
研究领域:
分析化学
·
色谱技术
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DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Mengsa Chai,Yihui Chen,Rongrong Xuan,Junfeng Ma,Tingting Wang,Dan Qiu,Lihua Zhang,Yukui Zhang
In current study, an attapulgite nanoparticles-based hybrid monolith was prepared by crosslinking 3-trimethoxysilylpropyl methacrylate modified attapulgite nanoparticles with acrylamide and N, N′-methylenebisacrylamide. The crosslinking of attapulgite into the hybrid monolithic matrix has notable increased the column efficiency of adenosine comparing with the neat one without attapulgite. The resulting hybrid monoliths showed good permeability and good mechanical stability. It was further applied for separation of polar compounds by hydrophilic interaction chromatography (HILIC). The key factors affecting the separation efficiency of the developed hybrid monoliths (i.e. acetonitrile content, salt concentration and pH in the mobile phase) have been completely investigated. The column efficiency was up to 147,613 plates/m for the HILIC separation of aspirin. Good repeatability of retention time was achieved, with relative standard deviations for run-to-run (n = 3), column-to-column (n = 3) and batch-to-batch (n = 3) in the range of 1.08–1.45%, 2.44–3.41% and 2.15–4.96%, respectively. We propose that the attapulgite nanoparticles-based hybrid monolith would provide a promising stationary phase for hydrophilic interaction chromatography.
研究领域:
分析化学
·
色谱技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）