MICROCHIMICA ACTA
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2011
·Vol. 175
·期 3
·355-359
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IF 2.578
Lu Wenbo,Qin Xiaoyun,Luo Yonglan,Chang Guohui,Sun Xuping
We demonstrate that CdS quantum dots (QDs) can be applied to fluorescence-enhanced detection of nucleic acids in a two-step protocol. In step one, a fluorescently labeled single-stranded DNA probe is adsorbed on the QDs to quench its luminescence. In step two, the hybridization of the probe with its target ssDNA produces a double-stranded DNA which detaches from the QD. This, in turn, leads to the recovery of the fluorescence of the label. The lower detection limit of the assay is as low as 1 nM. The scheme (that was applied to detect a target DNA related to the HIV) is simple and can differentiate between perfectly complementary targets and mismatches.
CdS quantum dots (CdSQDs) can serve as an effective sensing platform for fluorescence-enhanced DNA detection. This sensing system has a detection limit of 1 nM and is capable of differentiating between complementary and mismatched sequences.
研究领域:
生物化学
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分子生物学
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纳米技术
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DOI
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 26
·5234-5241
·
IF 1.915
Liu Yang,Yanfu Li
A new electrochemical biosensor based on the hybridization chain reaction and layered molybdenum selenide (MoSe2) stacked nanorod for dual-signal amplification is developed for the highly sensitive detection of the DNA sequences of Human Immunodeficiency Virus type 1 (HIV-1). The MoSe2 nanorod was prepared using a facile hydrothermal method. The glassy carbon electrode was first modified with MoSe2 nanorods and then Au nanoparticles (AuNPs) were electrodeposited on the electrode. The DNA probe was then immobilized on the electrode by an Au–S bond. As the initiator strands, auxiliary DNA can propagate a chain reaction of hybridization events between the two hairpin probes (Aux 1, Aux 2), and long nicked DNA polymers can be formed on the modified electrode. The biotin-labeled dsDNA polymers can then introduce numerous avidin-labeled horseradish peroxidases on the electrode, resulting in a significantly amplified electrochemical signal through the electrocatalysis of horseradish peroxidase. The target DNA is detected on the basis of the enzymatic oxidization of Fe2+ by H2O2. The electrochemical signals have a good linear relationship with the logarithm of target DNA concentration in the range from 10 fM to 0.1 pM, reaching a detection limit of the target DNA as low as 4.0 fM. This method shows good specificity for target DNA detection with the capability to discriminate single-base-pair mismatched DNA mutations. Moreover, the developed strategy was applied successfully in a real sample, which demonstrated the potential applications in clinical diagnostics.
研究领域:
生物传感技术
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分子诊断学
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产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
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2014
·
IF 2.736
Ling Li,Jin Zhang,Chao Li,Liqiang Chen,Renzhong Qiao
To efficiently deliver stavudine (d4T) for AIDS therapy, chitosan–stavudine conjugate (Cs–d4T) was synthesized. However, its poor water-solubility limited its clinical application. In this study, a sulphonated chitosan–stavudine conjugate (SCs–d4T) was synthesized with a mild SO 3 ·Py complex sulphonation strategy. Chemical characteristics and morphology of Cs–d4T and SCs–d4T were performed by NMR, XRD, FTIR, ICP-AES and SEM. SCs–d4T demonstrated satisfactory solubility (106-bold of Cs–d4T solubility), good anti-HIV activity (6-fold of d4T anti-HIV activity), and well sustained release ability. The major release product O -isopropyl-5′- H -phosphonate of d4T (d4T-P-H) showed higher anti-HIV activity than d4T. For further evaluating the influence of linker and sulphonation strategy on anti-HIV activity, chitosan grafted with d4T by succinyl linker (Cs–sd4T) and SCs–d4T sulphonated by oleum were also prepared. The result showed that the O -isopropyl monophosphate linker of Cs–d4T and SO 3 ·Py complex sulphonation strategy revealed higher anti-HIV activity than succinyl linker of Cs–sd4T and oleum sulphonation strategy, respectively.
研究领域:
药学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
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2020
·
IF 3.263
Yanzheng Xie,Lei Xie,Ailan Chen,Shengnan Wu,Yuxiao Mo,Xiaoqiang Guo,Changchun Zeng,Xinan Huang,Jinyang He
AIDS , a serious fatal disease caused by the human immunodeficiency virus (HIV), is an epidemic disease for which no effective vaccine has been established. The current therapeutic interventions for AIDS have limited efficacy because they are unable to clear HIV infections and the continuous occurrence of resistant HIV strains. Therefore, the exploitation of new drugs to prevent the spread of AIDS remains a high priority. In this study, the effects of icariin and its metabolite anhydroicaritin on SIV/HIV replication were investigated. In CEM × 174 cells and PBMC cells, both icariin and anhydroicaritin can significantly inhibit HIV-1 or SIVmac251 replication. Furthermore, molecular docking studies revealed that icariin and anhydroicaritin can act on both HIV reverse transcriptase and protease but could not bind to integrase . Reverse transcriptase and protease inhibition biological assays showed that both icariin and anhydroicaritin could significantly inhibit only HIV reverse transcriptase. In summary, the two compounds can significantly inhibit HIV/SIV in vitro and their targets may be mainly involved with HIV reverse transcriptase.
研究领域:
病毒学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
BME Frontiers
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2025
·
IF 7.7
Yirui Zhang,Jieyu Yan,Wangheng Hou,Qian Gao,Tao Zhang,Yan Gao,Jingwen Li,Kun Han
Objective: Accurate and rapid detection of disease biomarkers is critical for early diagnosis, timely intervention, and effective disease management. Impact Statement: This strategy is exemplified through the development of 2 biosensors for detecting HIV-1 DNA and HIV-1 p24, biomarkers associated with acquired immunodeficiency syndrome (AIDS). Introduction: We propose a general biosensing strategy that leverages a treble signal amplification cascade, demonstrating its versatility and applicability across diverse biomolecular targets. Methods: The method integrates multiple amplification mechanisms to achieve unparalleled sensitivity. Initially, the hybridization of 2 aided probes with the target triggers isothermal amplification facilitated by polymerase and nicking enzyme, providing a robust preliminary signal enhancement. Repeated cycles of primer extension, nicking, and signal primer dissociation then generate multiple signal primers. These primers are further amplified via rolling circle amplification (RCA), resulting in an important secondary signal boost. Finally, the amplified products activate CRISPR-Cas12a-mediated trans-cleavage, achieving a tertiary level of signal enhancement. Results: This cascade amplification approach achieves remarkable sensitivity, with detection limits of 62 aM for nucleic acids and 8.48 pg/ml for proteins, positioning it as a broadly applicable framework. Clinical samples were assayed, which indicates its capability in clinical diagnosis. Conclusion: Beyond HIV detection, the modular design of this strategy allows adaptation for various biomarkers, showcasing its potential as a universal platform for molecular diagnostics in healthcare and research.
研究领域:
生物传感器
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分子诊断
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病毒学
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临床诊断
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生物医学工程
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核酸扩增
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蛋白质检测
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产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2024
·Vol. 12
·期 19
·5091-5104
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IF 5.8
Yamin Chai,Wenyan Han,Yanjia Zhang,Yunzheng Du,Biao Wang,Mengya Chen,Nan Li,Wei Luo,Xiaoyu Zha,Lichun Wang,Lailiang Ou
Due to their rapid spread, high variability, and drug-resistant strains, new viral infections are continuously emerging. A lack of effective antiviral drugs and vaccines, resulting in disease and death, has significant socioeconomic consequences. Hemoperfusion can effectively adsorb and remove toxins from the blood, thus purifying the blood and serving as an acute treatment. Therefore, the aim of this study was to construct adsorbents to selectively remove viruses from the blood to quickly treat pathogen infection. We reported on new metal–organic framework (MOF)-polymer beads based on MIL-53(Al) and cellulose acetate (CNC), which were prepared by a one-step phase inversion method and applied as a viral hemo-adsorbent for the first time. The characterization results demonstrated that MIL-53(Al) was well dispersed in the CNC matrix. The adsorption results demonstrated that the capture efficiency of the human immunodeficiency virus (HIV) could exceed 99.93%, and the corresponding infectious titer decreased by approximately 103 times in clinical application. Moreover, CNC/MIL-53 exhibited low hemolysis ratios and good anticoagulant properties. Furthermore, molecular dynamics simulations revealed that the interplay of hydrogen bonding was the governing physisorption mechanism. Overall, CNC/MIL-53 could serve as a new type of hemoperfusion adsorbent for virus removal from blood and provide a new treatment pathway to mitigate epidemics.
研究领域:
生物医学工程
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材料科学
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病毒学
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医疗器械
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产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
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2018
·
IF 8.806
Yuling Mao,Shuang Feng,Shuai Li,Qinfu Zhao,Donghua Di,Yanfeng Liu,Siling Wang
Lymphatic transport of oral drugs allows extraordinary gains in bioavailability and efficacy through avoidance of first-pass hepatic metabolism and preservation of drugs at lymphatic tissues against lymph-mediated diseases. Chylomicrons can transport dietary lipids absorbed from the intestine to the tissues through lymphatic circulation . Herein, we engineered for the first time a chylomicron-pretended mesoporous silica nanocarrier that utilizes the digestion, re-esterification, and lymphatic transport process of dietary triglyceride to promote lymphatic transport of oral drugs. Taking lopinavir (LNV) as a model antiretroviral drug with disadvantages such as poor solubility, high first-pass effect and off-target deposition, this vehicle exhibited several properties belonging to ideal nanocarriers, including high drug load, amorphous dispersion and controlled release in the gastrointestinal tract . Additionally, a nano-bio interaction was demonstrated between nanoparticles and a key protein involved in chylomicron assembly; this biochemical reaction in cellular was utilized for the first time to promote lymphatic transport of nanocarriers for oral delivery. As a result, the chylomicron-pretended nanocarrier afforded 10.6-fold higher oral bioavailability compared with free LNV and effectively delivered LNV to gut-associated lymphoid tissues, where HIV persists and actively evolves. This approach not only promises a potential application to HIV-infected individuals but also opens a new avenue to other lymph-mediated pathologies such as autoimmune diseases and lymphatic tumor metastasis.
研究领域:
药剂学
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纳米医学
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药物递送系统
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免疫学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）