Journal of Chromatography Open
·
2022
·
IF 0
Anting Wang,Yuanhang Wang,Qin Huang,Xirenguli Hailiwuli,Lei Zhao,Zhaoyan Wang,Qiaosheng Pu
Capillary electrophoresis (CE) is a powerful separation tool for multicomponent analysis, but the necessity of switching the inlet end of the capillary between the vials of sample and running buffer for the sample injection complicates the operation and contributes to the irreproducibility, leading to poor compatibility with on-line analysis. Herein, coupling of capillaries with the aid of a plastic microchip was achieved for consecutive analysis. An assembly of 3 pieces of capillary with a cyclic olefin copolymer (COC) microchip was fabricated by thermal bonding of the COC plates with capillaries embedded in the micro-grooves with a cross-type configuration. Two capillaries acted as a sampling channel and the third capillary was used for separation. A buffer reservoir was installed on the microchip, which connected to the joint of the capillaries. The relative liquid levels of this reservoir and the vials at the other ends of the capillaries determine the length of the sample plug. With this assembly, the RSD of peak height and peak area for consecutive analysis were 0.54 % and 2.27 % (n = 9), the sample carryover was less than 0.32 %. The applicability of the device was confirmed by monitoring the leaching process of K + in a green tea and vitamin C effervescent tablets.
研究领域:
分析化学
·
生物医学工程
·
微流控技术
✓ 已验证
DOI
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Ningning Li,Ying Zhou,Hao Sun,Nan Wang,Nan Yang,Peiyao Ren,Lanjun Fu,Yiming Zhang,Wei Liu,Yanjie Li,Juan Jin
Rapid and accurate glucose monitoring is extremely crucial for the health management of diabetic patients. Whereas the non-invasive detection of salivary glucose is promising alternative. In this study, a newly designed MOF/MIPs paper-based microfluidic biomimetic chip (MM-SGM-chips) was successfully constructed based on boron-affinity orientation surface imprinting strategy. The obtained chips demonstrated high adsorption capability (Q e = 213.40 mg·g −1 ), excellent selectivity (IF = 3.7), speedy detection (less than 30 min), and satisfied anti-interference ability for glucose. Moreover, the MM-SGM-chips could be capable of rapid and sensitive quantification of glucose level ranged from 0.1-3.2 mM through visual detection, with a limit of detection as low as 0.02 mM. Furthermore, the real clinical samples in daily period were collected for validating its correlation with serum glucose level. Present works demonstrated the promising potentiality of MM-SGM-chips for home self-test and even point-of-care testing clinical applications.
研究领域:
生物医学工程
·
分析化学
·
糖尿病研究
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Lei Huang,Junsheng Hou,Li Ma,Zihan Ding,Yajie Yu,Junjie Wu,Xiaoling Yu,Wenjing Zhou,Zhenzhen Chen,Nanjing Hao
One-step continuous flow synthesis of nanofluids with superior heat transfer performance using microfluidic chips is an effective way to enhance flow-boiling heat transfer and address the thermal challenges of advanced electronic chips. However, the intricacy of conventional microfluidic chip fabrication techniques frequently results in assembly defects, flow channel seepage, and inhomogeneous mixing during the nanofluid synthesis process. In addition, the specific mechanisms of nanofluids as heat transfer media in enhancing the thermal management of electronic chips, particularly their impact on the dynamic behavior of bubbles during flow-boiling heat transfer, still require more in-depth studies. This study developed a three-dimensional helical microfluidic chip that exhibited excellent mixing performance under various operating conditions and achieved high-throughput continuous synthesis of silver nanofluids through combined simulation and experimental validation. The synthesized silver nanoparticles exhibited excellent colloidal stability (>60 days) and uniform nanoparticle size (<30 nm), and the size of silver nanoparticles could be controlled by adjusting the liquid flow rates of the inlets. The effects of nanofluid concentration and flow rate on flow-boiling heat transfer were investigated systematically. The results demonstrated that silver nanofluids showed significant enhancements, with the 0.01 wt % achieving a 65.66% increase in critical heat flux (CHF) and a 49.31% increase in heat transfer coefficient (HTC) at a flow rate of 160 mL/min. Additionally, visualization of the bubble’s growth cycles showed that silver nanofluids could effectively shorten the bubble’s lifetime, reduce the bubble’s size, and increase the number of nucleation sites. These findings provided a method for integrating microfluidic chips with electronic chip research through high-throughput synthesis of nanofluids.
研究领域:
材料科学
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热力学
·
微制造技术
✓ 已验证
DOI
PubMed
高影响因子
Micromachines
·
2020
·Vol. 11
·期 2
·120
·
IF 2.524
Yujun Chen,Tao Gong,Cilong Yu,Xiang Qian,Xiaohao Wang
Simplifying tedious sample preparation procedures to improve analysis efficiency is a major challenge in contemporary analytical chemistry. Solid phase microextraction (SPME), a technology developed for rapid sample pretreatment, has flexibility in design, geometry, and calibration strategies, which makes it a useful tool in a variety of fields, especially environmental and life sciences. Therefore, it is important to study the coupling between the microfluidic electrospray ionization (ESI) chip integrated with the solid phase microextraction (SPME) module and the electrospray mass spectrometer (MS). In our previous work, we designed a solid phase microextraction (SPME) module on a microfluidic chip through geometric design. However, automation and calibration methods for the extraction process remain unresolved in the SPME on-chip domain, which will lead to faster and more accurate results. This paper discusses the necessity to design a micromixer structure that can produce different elution conditions on the microfluidic chip. By calculating the channel resistances, the microfluidic chip’s integrated module with the micromixer, SPME, and ESI emitters optimize the geometry structure. We propose the annular channel for SPME to perform the resistances balance of the entire chip. Finally, for SPME on a single chip, this work provides a quantitation calibration method to describe the distribution of the analytes between the sample and the extraction phase before reaching the adsorption equilibrium.Keywords:flow-through solid phase microextraction (SPME) on chip;quantitative analysis of flow-through solid phase microextraction (SPME);online separation;eluted;microfluidic chip;mass spectrometer (MS)
研究领域:
分析化学
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环境科学
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生命科学
✓ 已验证
DOI
PubMed
高影响因子
STAR Protocols
·
2023
·
IF 0
Zitong Yu,Rui Hao,Xi Chen,Lu Ma,Yi Zhang,Hui Yang
Summary Organs-on-chips are microfluidic devices for cell culturing to simulate tissue- or organ-level physiology, providing new solutions other than traditional animal tests. Here, we describe a microfluidic platform consisting of human corneal cells and compartmentalizing channels to achieve fully integrated human cornea’s barrier effects on the chip. We detail steps to verify the barrier effects and physiological phenotypes of microengineered human cornea. Then, we use the platform to evaluate the corneal epithelial wound repair process. For complete details on the use and execution of this protocol, please refer to Yu et al. (2022). 1
研究领域:
生物医学工程
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组织工程
·
细胞生物学
✓ 已验证
DOI
PubMed
BIOMEDICAL MICRODEVICES
·
2018
·Vol. 20
·期 2
·1-7
·
IF 2.077
Jin Xin,Chen Rui,Zhao Shikun,Li Peiyong,Xue Bai,Chen Xiang,Zhu Xinyuan
In this article, an attempt for efficient screening of circulating tumor cells (CTCs) with excellent operability on microfluidic chips was reported. A Parsortix™-like cell separation chip was manufactured in our lab. This chip allowed lateral flow of fluid which increased the flow rate of blood. And, an air valve controlled injection pump was manufactured which allowed eight chips working simultaneously. This greatly facilitated the blood treatment process and saved time. As for the mechanism of screening circulating tumor cells, selective size amplification was utilized. By size amplification of cancer cells, both the hardness and the size of CTCs increased which differentiated them from blood cells. And the modification procedure of beads used for size amplification of cancer cells was optimized. Finally, by integrating the commercialized Parsortix™-like cell separation chip and selective size amplification, a practical method for screening circulating tumor cells was established.
研究领域:
医学工程
·
肿瘤学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
CANCER BIOLOGY & THERAPY
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2016
·
IF 2.921
Yu Zhang,Xiaofeng Zhang,Jinling Zhang,Bin Sun,Lulu Zheng,Jun Li,Sixiu Liu,Guodong Sui,Zhengfeng Yin
Circulating tumor cells (CTCs) have been proposed to be an active source of metastasis or recurrence of hepatocellular carcinoma (HCC). The enumeration and characterization of CTCs has important clinical significance in recurrence prediction and treatment monitoring in HCC patients. We previously developed a unique method to separate HCC CTCs based on the interaction of the asialoglycoprotein receptor (ASGPR) expressed on their membranes with its ligand. The current study applied the ligand-receptor binding assay to a CTC-chip in a microfluidic device. Efficient capture of HCC CTCs originates from the small dimensions of microfluidic channels and enhanced local topographic interactions between the microfluidic channel and extracellular extensions. With the optimized conditions, a capture yield reached > 85% for artificial CTC blood samples. Clinical utility of the system was further validated. CTCs were detected in all the examined 36 patients with HCC, with an average of 14 ± 10/2 mL. On the contrary, no CTCs were detected in healthy, benign liver disease or non-HCC cancer subjects. The current study also successfully demonstrated that the captured CTCs on our CTC-chip were readily released with ethylene diamine tetraacetic acid (EDTA); released CTCs remained alive and could be expanded to form a spheroid-like structure in a 3-dimensional cell culture assay; furthermore, sensitivity of released CTCs to chemotherapeutic agents (sorafenib or oxaliplatin) could be effectively tested utilizing this culture assay. In conclusion, the methodologies presented here offer great promise for accurate enumeration and easy release of captured CTCs, and released CTCs could be cultured for further functional studies.
研究领域:
肿瘤学
·
生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2021
·
IF 8.947
Kun Du,Shibo Li,Chengpan Li,Ping Li,Chunguang Miao,Tianzhi Luo,Bensheng Qiu,Weiping Ding
Nonalcoholic fatty liver disease (NAFLD) has emerged as a public health concern. To date, the mechanism of NAFLD progression remains unclear, and pharmacological treatment options are scarce. Traditional animal NAFLD models are limited in helping address these problems due to interspecies differences. Liver chips are promising for modeling NAFLD. However, pre-existing liver chips cannot reproduce complex physicochemical microenvironments of the liver effectively; thus, NAFLD modeling based on these chips is incomplete. Herein, we develop a biomimetic liver lobule chip (LC) and then establish a more accurate on-chip NAFLD model. The self-developed LC achieves dual blood supply through the designed hepatic portal vein and hepatic artery and the microtissue cultured on the LC forms multiple structures similar to in vivo liver. Based on the LC, NAFLD is modeled. Steatosis is successfully induced and more importantly, changing lipid zonation in a liver lobule with the progression of NAFLD is demonstrated for the first time on a microfluidic chip. In addition, the application of the induced NAFLD model has been preliminarily demonstrated in the prevention and reversibility of promising drugs. This study provides a promising platform to understand NAFLD progression and identify drugs for treating NAFLD. Statement of significance Liver chips are promising for modeling nonalcoholic fatty liver disease. However, on-chip replicating liver physicochemical microenvironments is still a challenge. Herein, we developed a liver lobule chip with dual blood supply, achieving self-organized liver microtissue that is similar to in vivo tissue. Based on the chip, we successfully modeled NAFLD under physiologically differentiated nutrient supplies. For the first time, the changing lipid zonation in a single liver lobule with the early-stage progression of NAFLD was demonstrated on a liver chip. This study provides a promising platform for modeling liver-related diseases.
研究领域:
生物医学工程
·
组织工程
·
药理学
·
肝脏疾病模型
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MICROELECTROMECHANICAL SYSTEMS
·
2025
·
IF 3.1
Zhengrui Hu,Han Wang,Shan Li,Youjiang Liu,Chunjing Xu,Shaomin Liu,Jianhui Wei,Chilai Chen
By introducing the strengths of the vertical interconnection of TGV (Through Glass Via) technology into FAIMS (high-Field Asymmetric waveform Ion Mobility Spectrometry) chip manufacturing, we propose a wafer-level TGV-MS (multi-stack) FAIMS chip manufacturing method and achieve the wafer-level fabrication of 14 chips on a 6-inch glass wafer for the first time. The test results demonstrate that the TGV wafer manufactured based on this method shows excellent airtightness, electrical properties, and consistency with a leakage rate of $3.94\times 10 ^{-11}$ Pa $\cdot $ m3/s, a resistivity of $1.92\times 10 ^{-4}~\Omega \cdot $ cm. A single FAIMS chip volume is $4.8\times 1.6 \times 0.16$ cm3, weight is 2.28 g, the relative thickness error is less than 0.3%, and the parallelism error is less than 0.01°. Meanwhile, typical volatile organic compounds benzene and toluene were selected as samples to test the separation performance of the FAIMS chip, and the detection spectra under different gas concentrations and voltages were obtained. The experimental results show that the wafer-level FAIMS chip has a good resolution for benzene and toluene, and the linear detection range for the two samples is 0.1 ppm to 2 ppm (linear fitting coefficients 98.9%, 99.5%). The detection limit for benzene and toluene is 77 ppb. This paper solves the problem that microfluidic structures with electrodes cannot be manufactured at the wafer level due to side planar electrode leads, greatly simplifies the structure of the chip, realizes the mass production of FAIMS chips, and provides a technical reference for the wafer-level manufacturing of analysis sensors such as microfluidic chips with electrode structures and MEMS mass analyzers. [2024-0229]
研究领域:
微制造技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Jing Zhang,Zishuo Fang,Heng Dong,Yu Liu,Zhenyu Chu,Yongbin Mou,Wanqin Jin
In clinical settings, saliva has been established as a straightforward, noninvasive medium for diagnosing periodontitis. However, the precise diagnosis is often hampered by the absence of a specialized analyzer capable of detecting low concentrations of biomarkers typically found in saliva. In this study, we present a noninvasive, on-site screen-printed biomicrochip specifically engineered for the precise and sensitive quantification of lactate concentrations in saliva, a critical biomarker in the diagnosis of periodontitis. The microchip is constructed using a nanostructured ink formulation that includes MnFe@N-doped carbon nanotubes (MnFe@N-CNTs). These MnFe@N-CNTs exhibit a high degree of graphitization and low electrical resistance, significantly augmenting the electrocatalytic efficiency of the enzymatic reaction of lactate. This results in doubled sensitivity and a detection limit that surpasses those of the current advanced salivary assay methods. Remarkably, within just 30 s, the biomicrochip can quantitatively and precisely measure lactate concentrations in the saliva of 10 patients, which provides valuable insights into the severity of their periodontitis. This biosensor holds excellent potential for large-scale production and could broaden the scope of biomarker recognition, paving the way for the analysis of a wider range of oral diseases.
研究领域:
生物医学工程
·
临床诊断
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2016
·
IF 0.566
Da-Peng GUO,Yong-Huan WANG,Tian-Bai XU,Yuan ZHANG,Ling-Feng Li,Jin-Kai CHEN,Xiao-Zhi WANG,Peng LI,Ji-Kui LUO
The microchip field asymmetric ion mobility spectrometry (FAIMS) technology was used for rapid analysis of phenylalanine (PHE) using the metal diffusion tube-microchip μ-FAIMS system. The test pressure was set at 0.25 MPa, and the temperature of the gas generator for the diffusion tube was at 190 °C. The ion characteristic spectra were acquired under the optimized conditions with a gas flow rate of 2000 mL min −1 , a dispersion voltage of 152.8 V and a compensation voltage of −0.62 V for phenylalanine in positive mode. Phenylalanine gas samples with different concentrations were analyzed in detail by FAIMS. The results showed that the ion intensity was linearly correlated with phenylalanine concentration in the range from 6 mg L −1 to 20 mg L −1 , with the detection limit of 5.93 mg L −1 for phenylalanine. This investigation shows the feasibility of FAIMS technique for rapid detection of phenylalanine. The FAIMS instrument is simple, and has high sensitivity and fast response, and shows great potential for rapid detection of phenylalanine.
研究领域:
分析化学
·
医学检验
✓ 已验证
产品关联
DOI
高影响因子
Biotechnology Journal
·
2024
·Vol. 19
·期 2
·2300390
·
IF 3.2
Yaqiong Guo,Yingying Xie,Jianhua Qin
Organ-on-a-chip technology has shown great potential in disease modeling and drug evaluation. However, traditional organ-on-a-chip devices are mostly pump-dependent with low throughput, which makes it difficult to leverage their advantages. In this study, we have developed a generic, pump-free organ-on-a-chip platform consisting of a 32-unit chip and an adjustable rocker, facilitating high-throughput dynamic cell culture with straightforward operation. By utilizing the rocker to induce periodic fluid forces, we can achieve fluidic conditions similar to those obtained with traditional pump-based systems. Through constructing a gut-on-a-chip model, we observed remarkable enhancements in the expression of barrier-associated proteins and the spatial distribution of differentiated intestinal cells compared to static culture. Furthermore, RNA sequencing analysis unveiled enriched pathways associated with cell proliferation, lipid transport, and drug metabolism, indicating the ability of the platform to mimic critical physiological processes. Additionally, we tested seven drugs that represent a range of high, medium, and low in vivo permeability using this model and found a strong correlation between their P app values and human Fa, demonstrating the capability of this model for drug absorption evaluation. Our findings highlight the potential of this pump-free organ-on-a-chip platform as a valuable tool for advancing drug development and enabling personalized medicine.
研究领域:
生物医学工程
·
药理学
·
组织工程
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）