Pollen grain was explored as a new carrier for enzyme immobilization. After being modified with boric acid-functionalized titania, the pollen grain was able to covalently immobilize glycosylated enzymes by boronate affinity interaction under very mild experimental conditions (e.g., pH 7.0, ambient temperature and free of organic solvent). The glucose oxidase and horse radish peroxidase-immobilized pollen grain became a bienzyme system. The pollen grain also worked as an indicator of the cascade reaction by changing its color. A rapid, simple and cost-effective approach for the visual detection of glucose was then developed. When the glucose concentration exceeded 0.5 mM, the color change was observable by the naked eye. The assay of glucose in body fluid samples exhibited its great potential for practical application.Keywords:enzyme immobilization;boronate affinity;pollen grain;glucose detection
Albahi Amgad,Korin Ali,Elkhedir Abdeen,Elsir Elbasheer,Wu Di,Liang Hongshan,Li Bin
This study investigated the potential of using sunflower pollen grains (SPGs) as a carrier for soybean peptides (SoyP) to improve their palatability by masking their bitterness. SoyP is a bioactive macromolecule with various nutritional and medicinal benefits, but the high bitterness makes it unappealing to many consumers. To address this issue, the researchers encapsulated SoyP within SPGs using sodium alginate (SA) as a coating material. The resulting SoyP (Pellet) were analyzed using various techniques, including SEM, CLSM, FTIR, DSC, and XRD. The effectiveness of this taste-masking approach was evaluated using an E-tongue and dissolution under simulated oral conditions. The study found that SoyP (Pellet) exhibited significantly reduced bitterness and increased palatability compared to unencapsulated SoyP due to the moderate interaction between SoyP, SPGs, and SA. The use of sporopollenin microencapsulation presents a promising strategy for masking bitterness in nutraceutical applications and could lead to the development of more palatable functional foods and supplements.
Small extracellular vesicles (EVs) have various functions through the transfer of specific biomolecules. However, it is still challenging to capture small EVs with high sensitivity and specificity. Herein, inspired by the unique burry structure and the strong adhesion ability of pollen grains, we presented a novel Fe 3 O 4 @MgSiO 3 hierarchical magnetic nanoparticles (HNPs) as nanocarriers for the capture of small EVs. The NPs were generated through the solvothermal method and further modified with branching dendrimers to exhibit a hierarchical morphology. The enlarged surface area facilitated high-efficient capture of small EVs through specific recognition of aptamer probes and the small EVs surface markers. Besides, the magnetic core of the NPs allowed them to be isolated under the action of an external magnetic field, and thus the captured small EVs could be easily separated from plasma. These results indicated that the HNPs could serve as excellent nanocarriers for small EVs capture and related biomedical applications.
危险品化学品经营许可证（带存储）