JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 3
·824-833
·
IF 2.479
Yue Yang,Mingchang Liu,Kai Wang,Yange Yang,Ning Su,Wensheng Huang,Yajun Wu
The crude flavonoid extract of pollen (CFP) of four species of honeybee pollens were extracted with ethanol, and the total flavonoid contents ranged from 3.4 to 14.5 mg rutin/g dry weight. The antioxidant activities of the CFPs were evaluated from both chemical and cytological aspects. Comprehensive antioxidant scores were determined based on these two evaluation systems. The results showed that canola CFP had the highest antioxidant capacity among the four CFPs. A cytotoxicity assay was conducted to assess the safety threshold of the CFPs, and canola CFP was proved to be the least toxic to vascular endothelial cell. Of the four tested CFPs, this research suggests that canola CFP is the most promising natural antioxidant. In addition, high-performance liquid chromatography (HPLC) analysis detected seven flavonoid glycosides in the hydrolysates of the four CFPs. Among them, quercetin and kaempferol were present in all four honeybee pollen extracts, but there were significant differences between their contents. A correlation analysis revealed a strong correlation between the content of quercetin in the pollen extract and the extract's antioxidant activity. Practical Application Many varieties of honeybee pollen are commercially available. The results of this study help guide consumers to choose honeybee pollens that have a better antioxidant effect. This report can also provide guidance and data in support of the development of honeybee pollen health products.
研究领域:
农业
·
食品科学
·
药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW PHYTOLOGIST
·
2025
·Vol. 245
·期 6
·2512-2526
·
IF 8.1
Lei Li,Qianqian Hu,Yi Zhao,Ting Jiang,Huaihao Yang,Binglian Zheng
Summary In plants, sperm cell formation involves two rounds of pollen mitoses, in which the microspore initiates the first pollen mitosis (PMI) to produce a vegetative cell and a generative cell, then the generative cell continues the second mitosis (PMII) to produce two sperm cells. DUO1, a R2R3 Myb transcription factor, is activated in the generative cell to promote S-G2/M transition during PMII. Loss-of-function of DUO1 caused a complete arrest of PMII. Despite the importance of DUO1, how DUO1 is regulated is largely unexplored. We previously demonstrated that ARID1, an ARID transcription factor, stimulates DUO1 transcription. Here, we show that cell proliferation suppressor RBR1 interacts with ARID1 to stabilize DUO1. While the C-terminus of RBR1 is dispensable for vegetative growth, it plays a crucial role in reproductive development and facilitates interaction with ARID1. Moreover, DUO1 is a short-lived protein, ARID1 promotes the RBR1–DUO1 interaction, and RBR1 stabilizes DUO1 in a proteasome-dependent manner. Thus, RBR1 promotes DUO1-dependent PMII progression via antagonizing its repressive role in the cell cycle factors CDKA;1 and CYCB1;1. Collectively, we uncover that ARID1 and RBR1 act in concert to regulate DUO1 at both the transcriptional and posttranscriptional levels, balancing cell specification and cell division.
研究领域:
植物学
·
遗传学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plant Reproduction
·
2021
·Vol. 34
·期 2
·91-101
·
IF 3.767
Jia Xin-Lei,Xue Jing-Shi,Zhang Fang,Yao Chi,Shen Shi-Yi,Sui Chang-Xu,Peng Yu-Jia,Xu Qin-Lin,Feng Yi-Feng,Hu Wen-Jing,Xu Ping,Yang Zhong-Nan
The pollen coat, which forms on the pollen surface, consists of a lipid–protein matrix. It protects pollen from desiccation and is involved in adhesion, pollen–stigma recognition, and pollen hydration during interactions with the stigma. The classical methods used for pollen coat observation are scanning and transmission electron microscopy. In this work, we screened a collection of fluorescence dyes and identified two fluorescent brighteners FB-52 and FB-184. When they were used together with the exine-specific dye, Basic fuchsin, the pollen coat and the exine structures could be clearly visualized in the pollen of Brassica napus . This co-staining method was applied successfully in staining pollen from Fraxinus chinensis , Calystegia hederacea , and Petunia hybrida . Using this method, small pollen coat-containing cavities were detected in the outer pollen wall layer of Oryza sativa and Zea mays . We further showed these dyes are compatible with fluorescent protein markers. In the Arabidopsis thaliana transgenic line of GFP-tagged pollen coat protein GRP19, GRP19-GFP was observed to form particles at the periphery of pollen coat . This simple staining method is expected to be widely used for the studies of the palynology as well as the pollen–stigma interaction.
研究领域:
植物科学
·
孢粉学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Crystals
·
2021
·Vol. 11
·期 9
·1049
·
IF 2.589
Jia Xiang,Bo Wang,Le Fu,Chuanpin Chen,Wenjie Liu,Songwen Tan
Lactose is a commonly used excipient with two isomers. Different isomers have different properties, especially in terms of solubility. This work is mainly to explore the influence of different a/β ratio lactose on drug dissolution. This work has developed novel mesoporous pollen-like lactose anhydrous with tailored α/β ratios as ingredient carriers for controlled dissolution rate. The produced lactose carriers are pollen-like with a particle size of ~15 μm and a mean pore width of ~30 nm. β-lactose anhydrous has a unique FTIR-peak at 948 cm−1, whereas α-lactose anhydrous shows a unique FTIR-peak at 855 cm−1. DSC analysis suggests that the pollen-like α/β-lactose crystals are polymorphs with unique peaks of melting points. XRD analysis suggests that (5:5)α/β-lactose polymorph has high crystalline purity. The loading efficiency (30.6–33.4%w/w) of acetamidophenol within the nanoporous lactose particles is dependent on the surface structure and pore volumes—the pore volumes were found to be 0.0209–0.0380 cm3/g. The release rates of acetamidophenol are lower for lactose with high α/β ratios. The lactose solubility and the first-order release constant can be tailored by changing the proportion of β-lactose in the pollen-like lactose carriers.Keywords:pollen-like anhydrous lactose;α/β ratio;solubility;controlled release
研究领域:
药剂学
·
材料科学
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Shilong Ma,Yang Yang,Zhongmin Fu,Qingyun Diao,Mengyue Wang,Qihua Luo,Xing Wang,Pingli Dai
Tropilaelaps mercedesae is not only a major threat to honey bees in Asia but also a potential risk to global apiculture due to trade. Imidacloprid is a systemic insecticide that negatively affects individual bees. Moreover, the health of honey bees may be threatened by imidacloprid exposure and T. mercedesae infestation. We studied the effects of T. mercedesae and imidacloprid on the survival, food consumption and midgut bacterial diversity of Apis mellifera in the laboratory . Illumina 16S rRNA gene sequencing was used to determine the bacterial composition in the honey bee midgut. T. mercedesae decreased survival in parasitized honey bees compared with nonparasitized honey bees, but there was no significant difference in food consumption. The imidacloprid 50 μg/L diet significantly decreased syrup consumption of A. mellifera compared with the control diet. The combination of T. mercedesae infestation and imidacloprid 50 μg/L exposure reduced survival and increased pollen consumption of A. mellifera . T. mercedesae infestation or a combination of T. mercedesae infestation and exposure to 25 μg/L imidacloprid affected the midgut bacterial composition of honey bees. T. mercedesae infestation and imidacloprid exposure may reduce the survival and affect honey bee health.
研究领域:
昆虫学
·
微生物学
·
生态毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2020
·Vol. 21
·期 24
·9529
·
IF 4.556
Shanxia Jin,Liping Liu,Mengying Fan,Yaru Jia,Ping Zhou
Pollen grain was explored as a new carrier for enzyme immobilization. After being modified with boric acid-functionalized titania, the pollen grain was able to covalently immobilize glycosylated enzymes by boronate affinity interaction under very mild experimental conditions (e.g., pH 7.0, ambient temperature and free of organic solvent). The glucose oxidase and horse radish peroxidase-immobilized pollen grain became a bienzyme system. The pollen grain also worked as an indicator of the cascade reaction by changing its color. A rapid, simple and cost-effective approach for the visual detection of glucose was then developed. When the glucose concentration exceeded 0.5 mM, the color change was observable by the naked eye. The assay of glucose in body fluid samples exhibited its great potential for practical application.Keywords:enzyme immobilization;boronate affinity;pollen grain;glucose detection
研究领域:
生物化学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
·
2021
·
IF 5.062
Pan Tian,Zhongjie Cai,Guowu Zhan,Jiale Huang,Qingbiao Li
The conversion of CO 2 into value-added chemicals is an effective way to mitigate the environmental problems caused by huge anthropogenic CO 2 emissions. Although In 2 O 3 /Pd catalyst possesses excellent catalytic performance in CO 2 hydrogenation to methanol, the formation of Pd-In alloy on the catalyst surface greatly reduces the yield of methanol. Herein, we adopt a natural biomaterial ( viz. , rape pollen) as a template to synthesize hierarchically structured bio-In 2 O 3 and bio-In 2 O 3 /Pd catalysts. In particular, we found that the pretreatment of pollen using hydrochloric acid (HCl) played a critical role in determining catalytic performance. A series of bio-In 2 O 3 were prepared by using the pollen templates with different etching degrees, and then the bio-In 2 O 3 -x/Pd catalysts (x denoted as the amount of HCl, mL) were prepared using an impregnation-reduction method. It was surprising that the CO 2 conversion of bio-In 2 O 3 –15/Pd catalyst was 8.2% with methanol selectivity of 74.7%, which was much better than the bio-In 2 O 3 –0/Pd (2.6% and 54.6%, respectively). The dramatic enhancement was due to that the acid-etched pollen template owns a hollow cage-like structure and abundant surface functional groups (-NH 2 and -COOH), which is beneficial to the growth of In 2 O 3 with a high amount of surface oxygen vacancies. Also, the bio-In 2 O 3 /Pd catalyst (prepared with untreated pollen template) was more prone to form Pd-In alloys as a result of the strong interaction between Pd with In 2 O 3 . In contrast, the In 2 O 3 spatial distribution of bio-In 2 O 3 –15/Pd was more uniform with an appropriate interaction of Pd with In 2 O 3 that inhibited the formation of Pd-In alloys. Even Pd loaded on commercial In 2 O 3 exhibited inferior performance (3.4% CO 2 conversion and 81% methanol selectivity) to bio-In 2 O 3 –15/Pd catalyst. Accordingly, the present work offers a green and efficient method to prepare supported In 2 O 3 /Pd catalysts by using the acid-etched pollen as a template.
研究领域:
催化
·
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Cong Wang,Jieying Xiu,Kunxi Lü,Yafeng Li,Mingdeng Wei
Na super-ion conductor type material Na 3 V 2 (PO 4 ) 3 has been widely researched as the cathode of sodium-ion batteries (SIBs) in recent years, but the unsatisfying cost of Na 3 V 2 (PO 4 ) 3 impedes its wide application in SIBs. In this study, iron element is used to replace part of vanadium in Na 3 V 2 (PO 4 ) 3 to reduce its expense, and pine pollen is applied for the first time as a very effective carbon source to improve the performance of Na 4 FeV(PO 4 ) 3 . The fabricated composite material achieves a capacity of 105 mA h g −1 under 0.2 C and fascinating cycling stability over 94 % under 2 C for 500 cycles and 98 % under 10 C for 1000 cycles. The excellent cycle performance is caused by the involvement of pine pollen that acts as a carbon matrix to enhance the electron conductivity and block the agglomeration of active material effectively, thus the well-dispersed nano sized Na 4 FeV(PO 4 ) 3 shortens the diffusion path of sodium ion and gains a remarkable rate capability. Moreover, the distinguished reversibility during the charge and discharge procedures is ascribed also to the robust structure of Na 4 FeV(PO 4 ) 3 . This work provides an efficient route to realize the economic cathode material of SIBs with good performance.
研究领域:
材料科学
·
能源存储
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 15
·21826-21838
·
IF 4.223
Pang Xiuyu,Li Chenyu,Zang Chuanjiang,Guan Lei,Zhang Peng,Di Chunxiang,Zou Nan,Li Beixing,Mu Wei,Lin Jin
An analytical method of simultaneous detection of ten insecticide residues in honey and pollen was established. The samples were purified with QuEChERS approach using new adsorbents and analyzed with UPLC-MS/MS. The results showed that both of graphene and carbon nanotubes were highly efficient adsorbents for the dSPE clean up to eliminate coextractives in the samples, and graphene was superior to carbon nanotubes for the detection of pesticide residues in honey and pollen samples. The proposed method was used to detect pesticide residues in 25 honey samples and 30 pollen samples which were randomly collected from more than ten provinces in China. All honey samples contain 1–27 μg/kg of chlorpyrifos residues. Only 4% of the honey samples were detected containing acetamiprid and imidacloprid, while the other seven pesticides were not detected. Chlorpyrifos residues were found in all pollen samples (5–66 μg/kg), among which twenty percent exceeded the maximum residue limits (MRLs, 50 μg/kg, European Commission Regulation). Most of the pollen samples containing pesticide concentrations higher than MRLs were collected from rape, followed by lotus, camellia, and rose. Besides, 36.7% and 33.3% of the pollen samples had imidacloprid and flupyradifurone higher than 5 μg/kg. A total of 26.7% pollen samples were detected containing bifenthrin, while none of the other six pesticides were detected in pollen samples.
研究领域:
分析化学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2020
·Vol. 25
·期 6
·1264
·
IF 3.267
Huifang Zhang,Rui Liu,Qun Lu
Phenolamines and flavonoids are two important components in bee pollen. There are many reports on the bioactivity of flavonoids in bee pollen, but few on phenolamines. This study aims to separate and characterize the flavonoids and phenolamines from rape bee pollen, and compare their antioxidant activities and protective effects against oxidative stress. The rape bee pollen was separated to obtain 35% and 50% fractions, which were characterized by HPLC-ESI-QTOF-MS/MS. The results showed that the compounds in 35% fraction were quercetin and kaempferol glycosides, while the compounds in 50% fraction were phenolamines, including di-p-coumaroyl spermidine, p-coumaroyl caffeoyl hydroxyferuloyl spermine, di-p-coumaroyl hydroxyferuloyl spermine, and tri-p-coumaroyl spermidine. The antioxidant activities of phenolamines and flavonoids were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays. It was found that the antioxidant activity of phenolamines was significantly higher than that of flavonoids. Moreover, phenolamines showed better protective effects than flavonoids on HepG2 cells injured by AAPH. Furthermore, phenolamines could significantly reduce the reactive oxygen species (ROS), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, and increase the superoxide dismutase (SOD) and glutathione (GSH) levels. This study lays a foundation for the further understanding of phenolamines in rape bee pollen.Keywords:pollen;antioxidant;oxidative stress;hydroxycinnamic acid amides
研究领域:
植物化学
·
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
·
2014
·Vol. 25
·期 11
·1956-1964
·
IF 1.253
Dong Jianwei,Cai Le,Zhu Xiufang,Huang Xing,Yin Tianpeng,Fang Haixian,Ding Zhongtao
Cornhusk, corncob, and stigma maydis were extracted with water, aqueous ethanol, aqueous methanol, and ethyl acetate, respectively. The total phenolic contents (TPC), total flavonoid contents (TFC), total ketosteroid contents (TKC), and antioxidant activities [2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and 2,4,6-tri(2-pyridyl)-1,3,5-triazine (TPTZ) radical-scavenging activity, reducing power, and ferric reducing-antioxidant power (FRAP)] of the above extracts were investigated. Their main antioxidant components were further determined and quantified by high performance liquid chromatography (HPLC). The results revealed that the aqueous ethanol and aqueous methanol were more efficient in extracting antioxidant constituents from cornhusk, corncob, and stigma maydis. Eight main antioxidant components were detected from above three by-products, and the contents of four main antioxidant components were determined. The present work revealed that the cornhusk and corncob showed similar high TPC, TFC, TKC, and antioxidant activities with stigma maydis, and could be used as potential candidates for the prevention of diseases related to various oxidant by-products of human metabolism.
研究领域:
植物化学
·
食品科学
·
药理学
✓ 已验证
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Fangtian Liu,Yang Wu,Min Zheng,Yifei Liu,Shiyi Cao,Yijin Qiu,Ze Zhao,Hongbing Deng
The biological and environmental hazards of nanoplastics are increasingly recognized, driving the need for effective and sustainable removal methods. Hydrophobic adsorbent materials with high surface areas are promising for capturing hydrophobic nanoplastics, yet achieving these properties in an eco-friendly, energy-efficient manner remains challenging. In this study, we turned to nature and proposed a synergistic hydrophilic-hydrophobic inlay strategy that employs hydrophilic bio-macromolecule β-chitin to assemble bio-microparticle pollen featuring inherent hydrophobicity and micro/nano-patterns. The resulting pollen-chitin sponge possesses a porous, hydrophobic architecture enriched with unique components such as aromatic rings and acetylamino groups. This assembly promotes strong interpenetration and interconnection between hydrophobic particles and hydrophilic lamellae, enhancing the interaction among hydrophobic pollen particles, water, and nanoplastics. The optimized arrangement of adsorption sites enables high-efficiency nanoplastics removal, achieving an adsorption capacity of up to 236.30 mg g −1 . The removal process is primarily driven by hydrogen bond interaction, hydrophobic interaction, physical interception, and π – π interaction. As a result, it successfully purified wastewater contaminated with multi-plastic particles and showed advantageous potential for removing various hydrophobic pollutants. Furthermore, sustainable recycling performance is demonstrated. The natural co-assembly system represents a practical, sustainable combination of multi-scale and multi-component biomass materials for mitigating hydrophobic pollution.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）