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Food Chemistry-X · 2024 · IF 6.5

Effect of osmanthus hydrolat on the aroma quality and volatile components of osmanthus black tea

Xin Meng,Fang Wang,Chao-Hong Fu,Lin Zeng,Zhen-Hua Chen,Qizhen Du,Zhi-Hui Feng,Jun-Feng Yin,Yong-Quan Xu

Osmanthus fragrans is an evergreen shrub with a pleasant fragrance and a wide range of applications in many fields. The condensed hydrolat obtained during the drying process of its fresh flowers was collected in a low-temperature vacuum environment and its sensory evaluation and volatile components were studied. The main aroma compounds in Osmanthus fragrans were dihydro- β -ionone, nonanal, β -cyclocitral, β -ionone, benzaldehyde, α -ionone, and 6-methyl-5-hepten-2-one, whose contents were used as the main evaluation criteria, and the hydrolats obtained under different scenting and drying times were compared. This process can effectively collect the aroma components in Osmanthus fragrans and the optimal drying conditions were 50 °C for 5 h. The hydrolat was used to provide the scent of osmanthus black tea, which had a fresher and mellower taste, while the fragrance of osmanthus was abundant. These results show that osmanthus hydrolat can be used to provide the scent of floral black tea. Chemical compounds studied in this article (−)-Catechin (PubChem CID: 1203); (−)-epigallocatechin gallate (PubChem CID: 65064); (−)-epicatechin gallate (PubChem CID: 367141); (−)-epigallocatechin (PubChem CID: 72277); (−)-epicatechin (PubChem CID: 72276); (−)-gallocatechin gallate (PubChem CID: 199472); (−)-catechin gallate (PubChem CID: 6419835); (−)-gallocatechin (PubChem CID: 9882981).

CAS / MDL: 75-05-8 64-19-7
研究领域: 食品科学 · 植物学
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PLANTA · 2017 ·Vol. 246 ·期 2 ·277-280 · IF 3.361

Genetically engineered orange petunias on the market

Bashandy Hany,Teeri Teemu H.

Main conclusion Unauthorized genetically engineered orange petunias were found on the market. Genetic engineering of petunia was shown to lead to novel flower color some 20 years ago. Here we show that petunia lines with orange flowers, generated for scientific purposes, apparently found their way to petunia breeding programmes, intentionally or unintentionally. Today they are widely available, but have not been registered for commerce.

研究领域: 植物学 · 遗传学
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BMC PLANT BIOLOGY · 2025 ·Vol. 25 ·期 1 ·1-20 · IF 4.8

The chromosome-level genome of Hemerocallis middendorffii provides new insights into the floral scents and color biosynthesis in Chinese native daylily

Sun Meiyu,Han Baocai,Xiao Jianhua,Yu Haiyan,Jia Yinli,Yuan Shuhai,Yao Juan,Xing Quan,Wu Dongqi,Sun Guofeng,Zhang Jinzheng

Background  Hemerocallis middendorffii is a kind of high quality ornamental plant with strong cold resistance, pleasant aroma, and gorgeous floral color. Hemerocallis is one of the three internationally recognized perennial flowers that has huge demand in the flower market. However, little is known about the floral scent and color biosynthesis in daylily due to the large genome size. Results We reported here a high-quality chromosome-scale genome of H. middendorffii which was a native daylily in China. Using PacBio HiFi and Hi-C sequencing technologies, we assembled a chromosome-level genome of 4.10 Gb with a contig N50 of 12.81 Mb. We identified 65,536 protein-coding genes across the 11 chromosomes. We employed a combination of genomic, transcriptomic, and metabolomic approaches to investigate mechanisms behind the floral scent and color biosynthesis in daylily. The main terpenoids contributing to floral fragrance were linalool, 3-carene, nerolidol, and α-farnesene, while carotenoids and anthocyanidins were the main components of floral color. These traits could be stably inherited by their hybrid progenies, and some of them exhibited heterosis. Through Weighted Gene Co-expression Network Analysis (WGCNA) analysis, we identified two modules MEblueviolet and MEgreenyellow related to terpenoids (floral scents) and carotenoids (floral colors) biosynthesis. The 10 hub genes including DXS , MVD , MVK , DXR , LCYBs , Z-ISO , CYPs , and JAS were screened by network construction. The results of qRT-PCR analysis showed that these hub genes were significant differentially expressed in daylily samples, and these results were basically consistent with the relative transcript levels of RNA-seq. Conclusions This study not only provides new insights into the molecular mechanisms of floral scent and color, but also lays a foundation for subsequent research on key gene functions and molecular breeding in Hemerocallis .

CAS / MDL: 589-98-0
研究领域: 植物学 · 遗传学 · 生物化学
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FOOD CHEMISTRY · 2022 · IF 9.231

Quantitative conversion of free, acid-hydrolyzable, and bound ellagic acid in walnut kernels during baking

Xin Guo,Fengying Gu,Tingting Yang,Zhixiao Shao,Qiaozhen Zhang,Jinjin Zhu,Feng Wang

Ellagitannins, the main components of walnut kernel polyphenols, are easily degraded by heating to produce ellagic acid, precursor in the production of urolithins that show multiple physiological functions. To analyze the conversion of ellagitannins to free ellagic acid in walnut kernels during baking, a quantitative method was developed to investigate the ellagic acid content (EAC) in free phenolic acid (FPA), acid-hydrolyzable phenolic acid (AHPA), and bound phenolic acid (BPA) fractions. The results showed that the EAC in FPA reached its maximum (5.17 ± 0.30 mg/g DW) after baking at 165 °C for 30 min, which increased by 99.52% compared with the control. Meanwhile, the content of ellagitannins (ETC) in AHPA and BPA dropped by 89.14% and 26.08%, respectively. It suggested that baking promoted the conversion of ellagitannins in AHPA and BPA to ellagic acid in FPA. Eight ellagitannins were regarded as the main precursors of ellagic acid in walnut kernels.

研究领域: 食品科学 · 植物化学
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SCIENTIA HORTICULTURAE · 2022 · IF 4.342

Analysis of physicochemical and antioxidant properties of Malus spp. petals reveals factors involved in flower color change and market value

MeiLing Han,YuHeng Zhao,JiaXin Meng,Jiao Yin,HouHua Li

Flower color is the most important ornamental character trait of Malus species. The study aimed to clarify the roles of phenolic compounds and compositions in the petal color change of Malus spp. cultivars at various flowering stages. Meanwhile, the antioxidant activity in the petals of Malus spp. was detected to estimate the potential of market application. Eight Malus spp. cultivars were divided into four systems according to the flower color, namely dark-red (‘Royalty’ and ‘Perfect Purple’), rose-red (‘Kelsey’ and ‘Sparkler’), pink-red (‘Strawberry Parfait’ and ‘Pink Spires’), and white (‘Jewelberry’ and ‘Snow Drift’). Total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), and antioxidant activity of the petals were measured. The results revealed that high levels of TPC, TFC, TAC, and antioxidant activity in petals significantly decreased during flower development. These indicators were significantly and positively related to each other. Abundant phenolic metabolites in petals were determined by HPLC-DAD, and these compounds included anthocyanins, flavonols, flavanols, flavonones, phenolic acids, and dihydrochalcones. Anthocyanin concentrations and ratios in petals were notably different among the four color systems, with the highest levels noted in dark-red colored flowers, followed by rose-red, pink-red, and white flowers. Cyanidin-3- O -galactoside was the major anthocyanin noted. Its concentration reduced significantly with flower development, which is described as the primary cause of petal color fading. In addition, the petals contained abundance quercetin-3- O -glucuronide, hyperoside, taxifolin, chlorogenic acid, ferulic acid, phloridzin and other substances known for their antioxidant activities. Given these findings, we propose that the flowers of Malus spp. hold good utilization value as new sources of bioaccessible polyphenols and strong potential for development. Especially, the flowers at early stages will be supplements to the natural colorant market.

CAS / MDL: 53188-07-1
研究领域: 植物学 · 植物化学 · 农学
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Open Chemistry · 2019 ·Vol. 17 ·期 1 ·841-848 · IF 1.512

Comparative study on the antioxidant activities of ten common flower teas from China

Xiao-Fang Hu,Zong-Bao Ding,Yue Chen,Jiang-Fu Luo,Jian-Min He,Tian-Peng Yin

Flower teas are widely consumed across the world because of their beneficial health effects. The antioxidant activities of methanol extracts from ten common flower teas in China were evaluated using four antioxidant assays. The total phenolic (TPCs) and total flavonoid contents (TFCs) were also investigated. Most of these flower teas exhibited potent antioxidant effects, of which rosae rugosae flos exerted the strongest antioxidant effects in four assays. Rosae rugosae flos also exerted the highest TPC, while fragrans showed the highest TFC. Correction analysis indicated that phenolics play a key role in the antioxidant effect of flower teas, while flavonoids were poorly correlated with antioxidant activity. The results supported the consumption of flower teas as functional foods and their application as sources of natural antioxidants in the food industry.

研究领域: 食品科学 · 药理学 · 植物学
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PLANT MOLECULAR BIOLOGY · 2017 ·Vol. 93 ·期 6 ·593-606 · IF 3.356

Transcriptomic and hormone analyses reveal mechanisms underlying petal elongation in Chrysanthemum morifolium ‘Jinba’

Wang Jingjing,Wang Haibin,Ding Lian,Song Aiping,Shen Feng,Jiang Jiafu,Chen Sumei,Chen Fadi

Key message Auxin regulates chrysanthemum petal elongation by promoting cell elongation. Transcriptomic analysis shows that auxin signal transduction may connect with other transcription factors by TCPs to regulate chrysanthemum petal elongation. Abstract As an ornamental species, Chrysanthemum morifolium has high ornamental and economic value. Petal size is the primary factor that influences the ornamental value of chrysanthemum, but the mechanism underlying the development of C. morifolium petals remains unclear. In our study, we tracked the growth of petals and found that the basal region of ‘Jinba’ petals showed a higher elongation rate, exhibiting rapid cell elongation during petal growth. During petal elongation growth, auxin was demonstrated to promote cell elongation and an increase in cell numbers in the petal basal region. To further study the molecular mechanisms underlying petal growth, the RNA-seq (high-throughput cDNA sequencing) technique was employed. Four cDNA libraries were assembled from petals in the budding, bud breaking, early blooming and full blooming stages of ‘Jinba’ flower development. Analysis of differentially expressed genes (DEGs) showed that auxin was the most important regulator in controlling petal growth. The TEOSINTEBRANCHED 1, CYCLOIDEA and PCF transcription factor genes ( TCPs ), basic helix-loop-helix- encoding gene ( bHLH ), glutaredoxin-C ( GRXC ) and other zinc finger protein genes exhibited obvious up-regulation and might have significant effects on the growth of ‘Jinba’ petals. Given the interaction between these genes in Arabidopsis thaliana , we speculated that auxin signal transduction might exhibit a close relationship with transcription factors through TCPs. In summary, we present the first comprehensive transcriptomic and hormone analyses of C. morifolium petals. The results offer direction in identifying the mechanism underlying the development of chrysanthemum petals in the elongated phase and have great significance in improving the ornamental characteristics of C. morifolium via molecular breeding.

研究领域: 植物学 · 分子生物学
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CARBOHYDRATE POLYMERS · 2020 · IF 7.182

Inhibition effect of three common proanthocyanidins from grape seeds, peanut skins and pine barks on maize starch retrogradation

Mengting Wang,Jianchu Chen,Shiguo Chen,Xingqian Ye,Donghong Liu

The inhibition effect of three common proanthocyanidins (PA) on the retrogradation properties of maize starch was investigated (including grape seed proanthocyanidins (GSPA), peanut skin proanthocyanidins (PSPA), and pine bark proanthocyanidins (PBPA)). Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) analysis showed that PA could significantly decrease the values of melting enthalpy of retrogradation ( ΔH r ) and the degree of relative crystallinity, suggesting that the starch re-crystallization was retarded by PA. Scanning electron microscope (SEM) characterizations illustrated that retrograded PA-starch samples formed a looser matrix with less appearance of continuous flakes during storage. Overall, 0.5 %–2.0 % of three PAs exhibited suppression of starch retrogradation after 21-day cooling storage, mainly resulting from the PA-starch interaction. Among them, PSPA showed the most substantial inhibition effect on starch retrogradation, which might be attributed to its structural features. This study suggested that PA could be a new type of inhibitor to suppress starch retrogradation.

CAS / MDL: 9005-25-8
研究领域: 食品科学 · 化学 · 农业科学
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Toxins · 2025 ·Vol. 17 ·期 4 ·181 · IF 4

Toxin Production by Alternaria alternata in Black Spot Disease of Chrysanthemum morifolium ‘Fubai’: Accumulation of Altenuene and Tenuazonic Acid in Flowers

Qingling Zhan,Lina Liu,Wenjie Li,Jingshan Lu,Jiafu Jiang,Fadi Chen,Ye Liu,Zhiyong Guan

Alternariaspecies produce diverse secondary metabolites that act as critical virulence factors during plant pathogenesis. In cultivation areas ofChrysanthemum morifolium‘Fubai’—a key cultivar for herbal tea—black spot disease caused byA. alternatamanifests as necrotic leaf lesions progressing to wilting. Despite this disease’s economic impact, information on its associated toxins is limited, and the types of toxins produced by the black spot pathogen ofChrysanthemum morifolium‘Fubai’ in particular remain unclear. Furthermore, whether toxins are present in the flowers when the leaves show symptoms is uncertain, but their inflorescence is not visibly infected. Using two previously characterizedA. alternatastrains (F16/F20) isolated from ‘Fubai’ in earlier studies, we demonstrated the concomitant production of altenuene (ALT) and tenuazonic acid (TeA) in both strains, with strain-specific yield variations (F20 TeA: 342.16 µg/mL vs. F16: 21.84 µg/mL; ALT: 0.28 µg/mL vs. 0.90 µg/mL). Time-course monitoring revealed coordinated accumulation of both toxins in inoculated petals, reaching 18.07 μg/g ALT and 2.59 µg/g TeA by day 9. Notably, these two toxins were detected only in flower samples from black spot-infected plants, and their concentrations correlated closely with disease severity in the leaves. Moreover, although the inflorescences did not display symptoms, both fresh and dried flowers retained detectable toxin levels. We established a technical system for the extraction and quantitative detection of the toxins ALT and TeA produced by the black spot pathogen in tea chrysanthemum. This work provides the first confirmation of ALT/TeA co-contamination inChrysanthemum morifolium‘Fubai’, revealing substantial dietary exposure risks through tea consumption. Our findings suggest that, from a food safety risk reduction perspective, integrated management strategies should be developed to minimize toxin contamination in tea chrysanthemum, including improved disease prevention measures and potential regulatory considerations.

CAS / MDL: 64-18-6
研究领域: 植物病理学 · 食品安全 · 次生代谢产物研究
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FOOD CHEMISTRY · 2022 · IF 9.231

Comprehensive characterization of ingredients in Crocus sativus L. from different origins based on the combination of targeted and nontargeted strategies

Wei Si,Lele Xiong,Han Zhou,Huimin Wu,Zhe Liu,Guoqiang Liu,Yanfang Liu,Aijin Shen,Xinmiao Liang

As a valuable medicinal and edible plant, C rocus sativus L. has had wide applications since ancient times. Herein, a comprehensive approach for characterization of constituents in saffron was established based on the combination of targeted and non-targeted strategies. A targeted UPLC-ESI/MS n strategy was applied for in-depth identification of crocins, and a non-targeted UPLC-ESI/MS 2 approach characterized other components. This integration strategy was used to analyze ingredients in 21 batches of saffrons from 6 origins. Forty-seven crocins belonging to 8 types were identified including 32 new crocins. Among them, 6 new compounds with specific structures were reported for the first time, i.e. trans -6(G, 2G), trans -4(GT, g), trans -3(GT), cis -3(GT), methyl ester- trans -2(G) and methyl ester- cis -2(G). Besides, 91 non-crocin components were identified including 43 new compounds. Based on systematic investigation of crocins and non-crocins, we found that crocins were the critical components to distinguish saffrons from different origins, especially between domestic and foreign samples.

研究领域: 药用植物学 · 分析化学 · 植物科学
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Coatings · 2023 ·Vol. 13 ·期 4 ·714 · IF 3.4

Development of Antimicrobial Microcapsules of Saffron Petal Essential Oil by Condensation Method and Its Excellent Binding on Cotton Fibers

Su Liu,Liuxin Shi,Mengyun Liu,Wei Chen,Qiang Cheng,Xiuli Song

In recent years, textiles with antimicrobial properties have attracted more and more attention. As natural antimicrobial agents, essential oils’ potential application value lies in their ability to provide textiles with antimicrobial functions. In this paper, organic solvent extraction (n-hexane, petroleum ether, ethanol) and steam distillation were used to extract saffron petal essential oil (SPEO). It was found that organic solvent extraction (ethanol) had the highest extraction rate and the most apparent bacteriostatic effect. SPEO-Ms were prepared using the composite condensation method with gelatin and chitosan. The microstructure, encapsulation efficiency, slow-release performance, infrared spectrum, and thermal stability of the SPEO-Ms were evaluated. The results showed that the microencapsulated essential oil had good bacteriostatic properties. Antimicrobial cotton fabric was prepared by impregnating microcapsules onto cotton fibers. The effects of the microcapsules’ concentration on the whiteness, air permeability, moisture permeability, and bacteriological inhibition of the fabric were investigated. The results revealed that SPEO-Ms have the potential to be used as a new antimicrobial agent in textiles.Keywords:saffron flower petal essential oil;microcapsule;antimicrobial cotton fabric;cotton fabric

CAS / MDL: 111-30-8
研究领域: 纺织科学 · 微生物学 · 植物化学
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KEY ENGINEERING MATERIALS · 2020 · IF 0

Application of Peanut Shell-Based Porous Materials for the Effective Removal of HCN in Cigarette Mainstream Smoke

Yi Peng Wang,Song Yang,Yun Zhen Jia,Pei Jian Sun,Xue Hui Sun,Hong Bo Wang,Jun Wei Guo,Xiao Bing Zhang,Hui Min Liu,Cong Nie

In this study, carboxymethyl peanut shell (CPS) was prepared, and then peanut shell-based porous material (PPS) was obtained by the complexation reaction of CPS with Cu2+. The carboxymethyl content of CPS increased gradually with the increase of the amounts of monochloroacetic acid and sodium hydroxide, and the Cu2+ loading amount of PPS increased gradually with the increase of carboxymethyl content. The scanning electron microscope results showed that PPS-1, PPS-2, PPS-3 (PPS-1~3) had many nanoscale pores. The nitrogen adsorption-desorption results suggested that PPS-1~3 were all mesoporous materials with an average pore diameter of about 10 nm, while their Brunauer-Emmet-Teller surface area and pore volume of PPS-1 to PPS-3 increased gradually. Compared with the control cigarette, the release of HCN in cigarette mainstream smoke could be effectively reduced with the use of PPS-1~3, the reduction rate of HCN per total particulate matter were 54.9%, 63.1% and 67.0%, respectively.

研究领域: 材料科学 · 环境科学 · 烟草研究
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